• Clinical and Experimental Pediatrics
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• v.55 no.11
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• pp.424-429
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• 2012

Purpose: Methods for quick and reliable detection of Streptococcus pneumoniae are needed for the diagnosis of pneumococcal disease and vaccine studies. This study aimed to show that sequential multiplex polymerase chain reaction (PCR) is more efficient than conventional culture in achieving S. pneumoniae -positive results. Methods: Nasopharyngeal (NP) secretions were obtained from 842 pediatric patients admitted with lower respiratory infections at Severance Children's Hospital in Korea between March 2009 and June 2010. For identification and serotype determination of pneumococci from the NP secretions, the secretions were evaluated via multiplex PCR technique with 35 serotype-specific primers arranged in 8 multiplex PCR sets and conventional bacteriological culture technique. Results: Among the results for 793 samples that underwent both bacterial culture and PCR analysis for pneumococcal detection, 153 (19.3%) results obtained by PCR and 81 (10.2%) results obtained by conventional culture technique were positive for S. pneumoniae. The predominant serotypes observed, in order of decreasing frequency, were 19A (23%), 6A/B (16%), 19F (11%), 15B/C (5%), 15A (5%), and 11A (4%); further, 26% of the isolates were non-typeable. Conclusion: As opposed to conventional bacteriological tests, PCR analysis can accurately and rapidly identify pneumococcal serotypes.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.4
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• pp.455-461
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• 2017

Objective: Polymorphisms occurring in the precursor region of microRNAs (miRNAs) affect the target gene and alter the biogenesis of miRNAs, resulting in phenotypic variation. The purpose of the study was to investigate the genetic effects of rs16681031 (C>G) mutation in the precursor region of gga-miR-1658 on the economic traits of the Gushi-Anka chicken F2 resource population. Methods: To explore the effect of miR-1658 polymorphisms on chicken economic traits, the SNP was genotyped by MassArray matrix-assisted laser desorption/ionization-time of flight mass spectrometry. The association between the SNP and chicken body size, growth and carcass traits was determined by linear mixed models. Results: The SNP was not only significantly associated with body weight at the age of 6, 8, 10, 12 weeks, respectively, but also with the breadth of the chicken chest, body slanting length and pelvic breadth at 4 weeks, chest depth at 8 weeks of age, and body slanting length at 12 weeks (p<0.05), respectively. Conclusion: Our data serve as a useful resource for further analysis of miRNA function, and represent a molecular genetic basis for poultry breeding.

• Journal of Environmental Science International
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• v.20 no.8
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• pp.1011-1019
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• 2011

The objective of this research is to evaluate optimal conditions of permeability and selectivity on the polysulfone membrane for efficiency of separation of $CH_4$ by checking four factors which are temperature, pressure, gas compositions and gas flow rates. When higher pressure was applied at the input, lower efficiency of recovery of $CH_4$ and higher efficiency of separation of $CH_4$ were shown. It has the tendency to show lower efficiency of recovery of $CH_4$ and higher efficiency of separation of $CH_4$ at the output as higher temperature at input. The lower flow rates make higher efficiency of recovery of $CH_4$ and lower efficiency of separation of $CH_4$. Finally, over 90% efficiency for $CH_4$ separation and recovery conditions are temperature ($-5^{\circ}C$), pressure (8 bar), gas composition rate (6:4) ($CH_4:CO_2$) and gas flow rate ($5\ell$/min). These conditions make higher separation and recovery efficiency such as 90.1% and 92.1%, respectively.

• Journal of Ginseng Research
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• v.20 no.1
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• pp.54-59
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• 1996

Red Ginseng extracts sol'n was sterilized at 85f for 20 mins and/or stored at 4$0^{\circ}C$ for 6 months and centrifuged for 20 mins at 8,500xg in order to investigate the changes in chemical components of supernatants and the properties of precipitates. Contents of crude saponin and ginsenoside-$Rb_1$, -$Rg_1$, -Re were partially decreased during heating and storage. Starch contents were decreased from 26.81% in red ginseng extracts to 17.50-8.81% in supernatants, whereas free sugar contents were increased from 15.50% to 20.29~21.35% by heating and storage. The contents of protein and minerals in supernatants were decreased, but acidic polysaccharides and polyphenol compounds were not changed. pH values of supernatants and precipitates were decreased. The absorbances of brown color precursor and brown pigment in precipitates, detected at 285 nm and 440 nm were remarkably increased. The Overa11 data suggest that precipitates in red ginseng extracts sol'n formed during steilization and storage are provably the brown pigments resulting from Maillard reaction of amino compounds with reducing sugar which could be released from starch and protein matrix and $Cu^+$, $Ca^{2+}$ and $Fe^{3+}$ ions are implicated with the reaction incorporated.

• Journal of the Korean Society of Tobacco Science
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• v.31 no.1
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• pp.45-50
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• 2009

In this study, a simple gradient RRLC method for rapid determination of carbonyl compounds of cigarette smoke was developed. Within 10 min, 8 carbonyl compounds have been separated and identified on ZORBAX Eclipse XDB-C18 column ($4.6{\times}50\;mm$, $1.8\;{\mu}m$) with gradient elution using water and acetonitrile as a mobile phase. RRLC was used for the quantification of carbonyl compounds in mainstream smoke of 3R4F reference cigarette, and evaluated those efficiencies in the recovery, repeatability and reproducibility. The correlation coefficients ($r^2$) for calibration curves of carbonyl compounds were over 0.9998. The developed RRLC method was successfully applied to the analysis of smoke samples and the recoveries of carbonyl compounds were in the range of 97.5~102.1% with RSD<3.1%.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.47 no.6
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• pp.901-907
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• 2014

We investigated the population ecology of blackthroat seaperch, Doederleinia berycoides, from samples collected in the southern seas of Korea from January to December, 2006. Population ecological parameters included survival rate, the instantaneous coefficient of natural and fishing mortality, and age at first capture. The survival rate (S) of blackthroat seaperch was estimated as 0.4966 using the catch curve method. The instantaneous coefficient of total mortality (Z) was 0.8598/year. The instantaneous coefficient of natural mortality (M) was estimated as 0.4694/year. From the estimates of Z and M, the instantaneous coefficient of fishing mortality (F) was calculated as 0.3904/year. The age at first capture ($t_c$), based on the Pauly method, was 2.87 years.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.6 no.2
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• pp.140-154
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• 1994

Experimental studies were performed to determine the characteristics of flow structure and pressure drop in 15 : 1 scale models of multi-louvered fin heat exchanger in a wide range of variables($L_P/F_P=0.5{\sim}1.23$, ${\theta}=27^{\circ}{\sim}37^{\circ}$, $Re_{LP}=50{\sim}2000$). Flow structure inside the louvered fin was analyzed by smoketube method and new correlations on flow efficiency and drag coefficient were suggested. The new definition for flow efficiency, which modifies the existing flow efficiency, can predict the flow efficiency in the range above mentioned and is represented as a function of Reynolds number, louver pitch to fin pitch ratio, louver angle at low Reynolds number. Drag coefficient which is defined here is a function of Reynolds number, louver pitch to fin pitch ratio, louver angle below critical Reynolds number, and can be represented by a function of louver pitch to fin pitch ratio only above the critical Reynolds number.

• The Bulletin of The Korean Astronomical Society
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• v.38 no.1
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• pp.42.1-42.1
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• 2013

In the LCDM paradigm, hierarchical merging is thought to play a key role in the formation and evolution of massive galaxies. Theoretical and observational studies suggest that massive galaxies started forming at high redshifts and were assembled via numerous mergers. Galaxy clusters are the sites where the most massive galaxies are found and the most dramatic merger histories are embedded. The previous work of Sheen et al. (2012) identified via visual inspection many massive galaxies with merger features in clusters, which surprised the community. In this study we aim to quantify peculiarity of galaxies to pin down the merger frequency in cluster environments more objectively. We have performed optical deep imaging of 4 Abell clusters by using IMACS f/2 on a Magellan Badde 6.5-m telescope. For the galaxies in our data, we applied GALFIT algorithm, which fits analytic models to galaxy data, and we analyzed their residuals. We present the preliminary results of our sample galaxies.

• Food Science and Biotechnology
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• v.18 no.3
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• pp.604-609
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• 2009

The antioxidative activities of the water solution of crude extract from edible mushroom enokitake Flammulina velutipes were compared with those of ascorbic acid and ascorbic acid 6-palmitate in oil-in-water (O/W) emulsions of cod liver oil. Oxidation of the emulsions was carried out at 40 and $50^{\circ}C$ in the dark. The antioxidant activities were measured by in vitro assay against oxygen uptake, 2-thiobarbituric acid value, hydroperoxide formation of the oils. Also, residual docosahexaenoic acid content was measured as indices of lipid oxidation. The cod liver oil in O/W emulsions with added enokitake crude extract (ECE) was significantly more stable against lipid oxidation than the control emulsions without the extract in terms of any oxidation indices used. Moreover, ECE provided remarkable antioxidative properties to eicosapentaenoic acid ethyl ester in emulsion system. These observations demonstrate that F. velutipes can be used as a natural antioxidant, which effectively prevents oxidation of polyunsaturated oils in emulsion system.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.21 no.7
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• pp.386-393
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• 2009

The present study has been studied on a thermal and flow characteristic of the microchannel waterblock with flow distributions in each channels. Results of a numerical analysis using the CFX-11 are compared with results of an experiment. Numerical analysis and experiment are conducted under an input power of 150 W, inlet temperature of $20^{\circ}C$ and mass flow rates of $0.7{\sim}2.0$ kg/min. Base temperature and pressure drop are investigated with standard deviations of mass flow rates in each channels of samples. The flow distribution and j/f factor of the sample 4 is increased by about 65.7% and 42.6%, compared to that of the reference model sample 3.