• Journal of Microbiology and Biotechnology
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• 제33권7호
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• pp.915-925
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• 2023

Sarcopenia is defined as loss of muscle mass and strength due to aging. Recent studies show that sarcopenia may improve via the gut-muscle axis, suggesting that gut health may affect muscle phenotypes. In this study, we aimed to investigate the ability of Lactobacillus rhamnosus JY02 as a probiotic strain isolated from kimchi to alleviate sarcopenia. L. rhamnosus JY02-conditioned medium (CM) reduced dexamethasone (DEX)-induced myotube diameter atrophy and expression of muscle degradation markers (MuRF1 and atrogin-1) in C2C12 cells. The amelioration of sarcopenia was investigated by measuring body composition (lean mass), hand grip strength, myofibril size (using histological analysis), and mRNA and protein expression of muscle-related factors in a DEX-induced mouse model. The results of these analyses showed that L. rhamnosus JY02 supplementation promoted the production of muscle-enhancement markers (MHC Iβ, MHC IIα, and Myo-D) and reduced both the production of muscle degradation markers and the symptoms of muscle atrophy (loss of lean mass and muscle strength). We also found decreased levels of pro-inflammatory cytokines (IL-6, IFN- γ) and increased levels of anti-inflammatory cytokines (IL-10) in the serum of DEX+JY02-administered mice compared to those in DEX-treated mice. Overall, these results suggest that L. rhamnosus JY02 is a potent probiotic supplement that prevents sarcopenia by suppressing muscle atrophy.

• 한국식품영양과학회지
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• 제46권1호
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• pp.39-45
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• 2017

본 연구에서는 생산지역별 감초의 물질 표준화를 위해 주요지표성분 중 glycyrrhizic acid를 지표물질로 선정하고 정량분석을 위해 분석법 검증을 수행하였다. 감초의 glycyrrhizic acid의 분석법 검증은 특이성, 직선성, 검출 및 정량한계, 정확성, 정밀성을 구하여 확립하였다. 확립된 분석 방법으로 시험한 국내외 생산지역별 감초의 glycyrrhizic acid 정량 분석 결과 국내산 중에서는 제천산이, 수입산 중에서는 우즈베키스탄산이 각각 16.98, 54.22 mg/g으로 높은 함량을 나타내었다. 또한, 현대인의 심각한 대사질환 중 하나인 당뇨병 예방 및 개선에 효과적인 기능성 천연물 소재를 발굴하고 항당뇨 효과 검증을 위해 생산지역별 감초 에탄올 추출물의 ${\alpha}-glucosidase$ 저해 활성 및 glucose uptake 활성을 평가하였다. ${\alpha}-Glucosidase$ 저해 활성은 국내외 5종 중 제천, 곡성지역의 감초에서 무처리군 대비 높은 저해 활성을 보였고, 우즈베키스탄산은 유의적인 활성이 나타나지 않았다. Glucose uptake 활성의 경우 인슐린 처리를 한 대조군 대비 제천, 영주, 우즈베키스탄 지역의 감초에서 각각 24.19, 27.18, 26.92% 유의적으로 증가함을 확인하였다. 따라서 본 연구의 지표성분 분석, 분석법 검증 결과 및 생리활성 평가는 생산지역별 감초의 물질 표준화 및 품질 평가의 기초자료를 제공하는 데 큰 의미가 있으며, 감초가 대사질환인 당뇨 예방 및 개선에 효과적인 생약식품 소재로의 개발 가능성을 확보하였다. ${\alpha}-Glucosidase$ 저해 활성 및 glucose uptake 활성 평가뿐만 아니라 감초에 함유된 많은 물질연구를 통해 알려지지 않은 활성에 관한 계속된 추후 연구가 필요할 것으로 보인다.

• Journal of Microbiology and Biotechnology
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• 제33권12호
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• pp.1635-1647
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• 2023

Muscle atrophy, which is defined as a decrease in muscle mass and strength, is caused by an imbalance between the anabolism and catabolism of muscle proteins. Thus, modulating the homeostasis between muscle protein synthesis and degradation represents an efficient treatment approach for this condition. In the present study, the protective effects against muscle atrophy of ethanol extracts of Morus alba L. (MA) and Angelica keiskei Koidz. (AK) leaves and their mixtures (MIX) were evaluated in vitro and in vivo. Our results showed that MIX increased 5-aminoimidazole-4-carboxamide ribonucleotide-induced C2C12 myotube thinning, and enhanced soleus and gastrocnemius muscle thickness compared to each extract alone in dexamethasone-induced muscle atrophy Sprague Dawley rats. In addition, although MA and AK substantially improved grip strength and histological changes for dexamethasone-induced muscle atrophy in vivo, the efficacy was superior in the MIX-treated group. Moreover, MIX further increased the expression levels of myogenic factors (MyoD and myogenin) and decreased the expression levels of E3 ubiquitin ligases (atrogin-1 and muscle-specific RING finger protein-1) in vitro and in vivo compared to the MA- and AK-alone treatment groups. Furthermore, MIX increased the levels of phosphorylated phosphoinositide 3-kinase (PI3K), protein kinase B (Akt), and mammalian target of rapamycin (mTOR) that were reduced by dexamethasone, and downregulated the expression of forkhead box O3 (FoxO3a) induced by dexamethasone. These results suggest that MIX has a protective effect against muscle atrophy by enhancing muscle protein anabolism through the activation of the PI3K/Akt/mTOR signaling pathway and attenuating catabolism through the inhibition of FoxO3a.