• 제목/요약/키워드: C. perfringens type A

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새끼 하마에서 Clostridium perfringens Type A 감염 증례 (Clostridium perfringens Type A Infection in a Hippopotamus amphibius Cub)

  • 김영섭;임숙경;신남식
    • 한국임상수의학회지
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    • 제25권4호
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    • pp.310-313
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    • 2008
  • C. perfringens is the most important enteric clostridial pathogen of animals. C. perfringens type A has been associated with hemorrhagic enteritis in a wide lange of domestic and wild mammals. But all types of C. perfringens can be normal inhabitants of the intestine of most mammals. We have a special case that showed C. perfringens type A infection in a hippopotamus (Hippopotamus amphibius) cub at Seoul Grand Park Zoo. Male, hippopotamus cub died in 3 days after birth. Clinical features of the hippopotamus cub have showed lethargy and anorexia before death. Gross post-mortem findings of the hippopotamus were hemorrhagic enteritis of intestine. Histopathologically, ruminant stomach and intestine showed hemorrhagic lesions and the lumen of the small intestine was filled with mucoid and hemorrhagic fluid. Also, intestine and stomach of hippopotamus were distended with gas and hemorrhagic fluid. C. perfringens was isolated in culture of small intestine and the presence of C. perfringens type A was confirmed by PCR. This case indicated that C. perfringens type A could be considered as a virulence factor responsible for causing death of a newborn hippopotamus.

Detection of Clostridium perfringens and its toxinotypes by enzyme linked immunosorbent assay from enterotoxaemic goats in Bangladesh

  • Islam, K.B.M.S.;Rahman, M.S.;Ershaduzzaman, Md.;Taimur, M.J.F.A.;Jang, Hyung-Kwan;Song, Hee-Jong
    • 한국동물위생학회지
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    • 제33권1호
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    • pp.37-44
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    • 2010
  • An enzyme-linked immnnosorbent assay (ELISA) has been performed for the detection of the prevailing toxinotypes of Clostridium perfringens obtained from conventional culturing of intestinal contents of goats which have died of suspected enterotoxaemia. The test was found effective to detect the toxins as well as types of the organism with less time and labor. The most prevailing type of C. perfringens causing enterotoxaemia in goat was C. perfringens type D (68.75%) and followed by C. perfringens type B (25%) and C (6.25%). No C. perfringens type A was detected. This study showed an intelligible picture of prevailing toxinotypes of C. perfringens in goats in Bangladesh. The use of the ELISA for the detection of clostridial types and toxins allows the differential diagnosis of C. perfringens types A, B, C and D enterotoxaemias from samples of intestinal contents and the typing of cultures of C. perfringens.

닭에서 분리한 Clostridium perfringens의 독소형 (Toxigenic type of Clostridium perfringens isolated from chicken in Korea)

  • 박경윤;이상운;유한상;예재길
    • 대한수의학회지
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    • 제36권4호
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    • pp.829-837
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    • 1996
  • Clostridium perfringens has been identified as a causative organism in necrotic enteritis in chicken. The bacterium has been classified into five toxigenic types (A through E) based on the pattern of the production of major lethal toxins. Seroneutralization with mice or guinea pigs usually has been used to type the organism. Of the types, types A and C of the bacterium had been recognized as the major pathogenic types in chicken. In this experiment, we isolated nine field strains of C perfringens from chicken showing necrotic enteritis in clinical symptoms and pathologic findings and identified by biochemical tests. In order to type the organism, a multiplex polymerase chain reaction (PCR) was used with primers on major lethal toxin genes instead of seroneutralization. Amplification of only a toxin gene with the PCR suggested that the disease in chicken was due to type A of C perfringens in Korea. Furthermore, the PCR method can be replaced with seroneutralization to type C perfringens in future.

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Antibiotic susceptibility of Clostridium perfringens type D isolated from feces of goats

  • Kim, Jun-Ho;Kim, Jeong-Hwa;Kim, Young-Hoan;Cho, Kwang-Hyun;Nam, Sang Yoon;Lee, Hu-Jang;Lee, Beom Jun
    • Journal of Preventive Veterinary Medicine
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    • 제42권4호
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    • pp.148-156
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    • 2018
  • Clostridium perfringens (C. perfringens) may cause diarrhea and enterotoxemia in adult and young livestock, leading to problems in the production and management of farms. Four hundred fecal samples were collected from 25 goat farms located in Gyeongsangbuk-do Province in the Republic of Korea. Sixteen C. perfringens strains were isolates from fecal samples, and the isolates were identified as type A (n=11) and type D (n=5). Additionally, ${\alpha}$- and ${\varepsilon}$-toxin genes were detected in 16 and 5 strains by PCR, respectively, and the enterotoxin gene was presented in 2 strains. The antibiotic susceptibility test was performed using the disk diffusion method and E-test method. In the disk diffusion method, ampicillin (n=16) and chloramphenicol (n=15) were highly susceptible to 16 C. perfringens isolates. In the E-test method, ampicillin, amoxicillin, amoxicillin/clavulanic acid and meropenem were susceptible to more than 14 of 16 C. perfringens isolates. This study indicates that administration of antibiotics such as ampicillin, amoxicillin/clavulanic acid and meropenem can prevent and treat C. perfringens infections in goats.

Clostridium perfringens type A associated enteritis in a Shitzu dog

  • Byun, Jae-Won;Yoon, Soon-Seek;Lim, Suk-Kyung;Lee, O-Soo
    • 한국동물위생학회지
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    • 제29권4호
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    • pp.497-501
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    • 2006
  • Acute hemorrhagic enteritis was diagnosed in a seven-month-old male Shitzu dog dying of blood stained diarrhea and vomiting. Clinical findings were anorexia, dullness and sudden death after massive bloody diarrhea. At necropsy, main lesion was the hemorrhage in small intestine, mainly duodenum and jejunum. Microscopically, Gram positive long bacilli were massively detected on the mucose epithelial cells and necrotic debris of small intestine. Coagulative necrosis of epithelial cells and thrombosis of small intestine were also identified. However, there was no lesion of crypt epithelium. Mineral infiltration in both gastric mucosa and renal tubules was detected and proliferation of fibrous tissue was also shown in corticomedullary regions. In bacterial examination, C perfringens was isolated in anaerobic culture and it was confirmed to type A by multiplex PCR. Therefore, the dog was diagnosed as C perfringens type A associated enteritis with uremia.

PCR-Based Detection and Molecular Genotyping of Enterotoxigenic Clostridium perfringens Isolates from Swine Diarrhea in Korea

  • Kim Sang-Bum;Lim Hyeong-Jun;Lee Wan-Kyu;Hwang In-Gyun;Woo Gun-Jo;Ryu Sang-Ryeol
    • Journal of Microbiology and Biotechnology
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    • 제16권2호
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    • pp.291-294
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    • 2006
  • Clostridium perfringens strains were isolated from swine diarrhea in Korea. Three out of nineteen (15.8%) isolates of C. perfringens were found to be enterotoxigenic by PCR analysis. PCR-based genotyping of the three enterotoxigenic isolates of C. perfringens revealed that they were types A, C and D, respectively. These results suggest that various types of enterotoxigenic C. perfringens can cause swine diarrhea, and that the presence of enterotoxigenic type A strain, known to be strongly associated with food poisoning, may cause public health problem in Korea.

시판 젓갈에서 분리한 Clostridium perfringens의 독소 유전자 및 항균제 내성 분석 (Toxin Genes and Antimicrobial Resistance of Clostridium perfringens Strains Isolated from Commercial Jeotgals)

  • 이신혜;박권삼
    • 한국수산과학회지
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    • 제56권6호
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    • pp.826-832
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    • 2023
  • Clostridium perfringens causes diarrhea and other diseases in humans and animals. We investigated the prevalence, toxin gene profiles, and antimicrobial resistance of C. perfringens isolated from commercial jeotgal sample. C. perfringens was isolated from 11 of 22 commercial jeotgals. All C. perfringens strains were positive for the alpha toxin gene, but not for the beta, epsilon, iota, CPE or NetB toxin genes; therefore, all strains were identified as type A C. perfringens. However, the beta2 toxin gene was identified in 54.5% of isolates. Disk diffusion susceptibility tests showed that most isolates were resistant to kanamycin (90.9%), nalidixic acid (72.7%), oxacillin (54.5%), erythromycin (27.3%), ciprofloxacin (9.1%) and clindamycin (9.1%). However, all strains were susceptible to 14 other antimicrobial including amoxicillin, ampicillin, and chloramphenicol. The average minimum inhibitory concentrations against C. perfringens of clindamycin, kanamycin, and nalidixic acid were 128.0, 128.0, and 54.0 ㎍/mL, respectively. These results provide new insight into the necessity for sanitation of commercial jeotgal, and provide evidence to help reduce the risk of contamination with antimicrobial-resistant bacteria.

일부 동물에서 분리한 Clostridium perfringens 독소형의 검색 (Detection for Toxin Types of Clostridium perfringens Isolates from an Animals)

  • 정희곤
    • 한국환경보건학회지
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    • 제24권3호
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    • pp.22-25
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    • 1998
  • Outbreak patterns were observed in 250 chickens of necrotic enteriris(0.3%) among 81,000, 90 enterotoxemic pigs(9.5%) among 950, and 9 enterotoxemic cattle(13.8%) among 65 in a farms of Gyeonggi, Chungnam, Kwangwon, and Chonnam area from 1993 to 1996. Clostridium perfringens was isolated from 36 strains(14.4%) among 250 chickens of necrotic enteritis, 21 strains(23.3%) among 90 enterotoxemic pigs, and 3 strains(33.3%) among 9 enterotoxemic cattle. Toxin types by the neutralization test were categorized as A-type in 36 strains(100.0%) among 36 chickens of necrotic enteritis, and as A-type in 17 strains(81.0%), as B-type in 2 strains(9.5%) and as C-type in 2 strains(9. 5%) among 21 enterotoxemic pigs, and also 3 strains(100.0%) among 3 enterotoxemic cattle.

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Clostridium perfringens 감염에 의한 한우 집단 폐사 증례 (Mass mortality of Clostridium perfringens infection in Hanwoo)

  • 이정원;김지영;곽길한;육현수;손구례;고원석
    • 한국동물위생학회지
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    • 제34권1호
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    • pp.31-35
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    • 2011
  • This is a case report that 24 heads of cattle suddenly died without clinical signs in a Hanwoo farm. The cause of death was Clostridium perfringens enterotoxemia resulted from them with feeding leftover food. The clinical signs were observed just before the death; increase of heart rate, shallow and rapid respiration, amyostasia, spasm and so forth. In autopsy, blood coagulation disorder, a little abdominal inflation, hepatomegaly and different size of red spots, congestion hemorrhage and undercurrent of bloody exudation were observed in the entire parts of small and large intestines. C. perfringens were isolated from the substantive organs, and a unique fragment of 405bp C. perfringens was amplified by PCR. Therefore, this case was diagnosed as enterotoxemia caused by ${\alpha}$-toxin of C. perfringens A type.

Comparison of Sensitivity of Detetion for Clostridium perfringens Type A Enterotoxin by the Reversed Passive Latex Agglutination and the Polymerase Chain Reaction

  • 정희곤
    • 한국환경보건학회지
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    • 제23권4호
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    • pp.45-49
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    • 1997
  • Clostridium perfringens A형이 생산하는 장독소를 검색해본 결과, RPLA법에 있어서는 2배로 희석한 용액으로부터 64배로 희석한 용액 (NCTC 8239 Hobbs serotype 3 CPE$^+$)에서까지 양성반응을 보였으며 PCR 기법에 있어서는 10 pg 희석 용액까지 364 bp의 장독소 DNA fragment(NCTC 8238 Hobbs serotype 2 CPE$^+$)를 확인 할수 있었다. 그러므로 장독소를 검색하기 위해서는 PCR기법이 RPLA법에 비하여 훨씬 감도가 높음을 확인할 수 있었다.

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