• Korean Journal of Medicinal Crop Science
• /
• v.18 no.3
• /
• pp.186-190
• /
• 2010

The chromosome number was identified and fluorescence in situ hybridization(FISH) mapping of 5S and 45S rDNAs were conducted for C. minima and C. lanceolata in the genus Codonopsis from Jeju island. In this study, we have confirmed that the somatic metaphase chromosome number determined as 2n=2x=16 was the same as the findings from the previous studies. While the conventional staining method makes it rather difficult to distinguish satellite chromosomes due to high degree of variability, FISH analysis produced the exact number and location of 5S and 45S rDNAs. Both species in the genus Codonopsis have a pair of 5S rDNA and their gene loci were observed on chromosome 3. Although two pairs of 45S rDNAs (one on chromosome 1 and the other on chromosome 8) were identified in both species, the 45S rDNA signals on chromosome 8 in C. minima were significantly weaker than those on chromosome 1. In addition, the 45S rDNA signals on chromosome 1 in C. lanceolata showed that the chromosome is non-homologus. In this study, we have determined cytogenetic characteristics of C. minima and C. lanceolata according to their gene replication patterns.

• Journal of the Korean Institute of Telematics and Electronics C
• /
• v.34C no.12
• /
• pp.54-60
• /
• 1997

A new method to neglect the third term of the computational energy expression in the single-layer neural network with feedback is introduced. The system often converges to local minima instead of to global minima, because the computational energy is not matched exactly with the cost function being optimized. One of the factors causing these tow functions different is the third term of computational enegy expression. Regarding this third term energy very small, it is always ignored in designing the system. However, a sthe system growing, this third term energy is also growing and this grown term makes the computational energy function much different from the cost function. In results of differency between two functions, system converges to local minima more than before. In this paper, a new method to neglect te third term energy is introduced, so that the system with tis new method has been imroved.

• ALGAE
• /
• v.34 no.1
• /
• pp.7-21
• /
• 2019

The genus Heterocapsa is one of the major dinoflagellate groups, with some of its species having worldwide distributions. However, prior to the present study, the phototrophic species Heterocapsa minima has been reported only from the northeast Atlantic Ocean. Recently, H. minima was found in the Korean waters, and a clonal culture was established. This culture was used to examine the morphology of the Korean strain H. minima HMMJ1604 through light and scanning electron microscopy, as well as for its genetic characterization. Furthermore, to determine the nationwide distribution of H. minima in Korea, its abundance was quantified in the waters of 28 stations in all four seasons in 2016-2018 using the quantitative real-time polymerase chain reaction method. The overall morphology of H. minima HMMJ1604 was very similar to that of the Irish strain H. minima JK2. However, the Korean strain had five pores around the pore plate, whereas the Irish strain had six pores. When properly aligned, the sequences of the large subunit and internal transcribed spacer regions of the ribosomal DNA of the Korean strain were identical to those of the Irish strain. This species was detected in the waters of 26 out of 28 stations, but its abundance was greater than $1.0cells\;mL^{-1}$ at 8 stations. The highest abundance of H. minima was $44.4cells\;mL^{-1}$. Although this species was found in all seasons, its abundance was greater than $1.0cells\;mL^{-1}$ when the water temperature and salinity were $10.9-25.0^{\circ}C$ and 17.5-34.1, respectively. To the best knowledge, the present study reported for the first time that H. minima lives in the Pacific Ocean and is widely distributed in the Korean waters.

• Journal of Microbiology
• /
• v.40 no.2
• /
• pp.129-133
• /
• 2002

The fungus, Emericellopsis minima W2, capable of degrading poly(3-hydroxybutyrate) (PHB) was isolated from a waste water sample. Production of the PHB depolymerase from E. minima W2 (PhaZ/ sub Emi/) was significantly repressed in the presence of glucose. PhaZ/ sub Emi/ was purified by column chromatography on Octyl-Sepharose CL-4B and Sephadex G-100. The molecular mass of the PhaZ/ sub Emi/), which consisted of a single polypeptide chain, was estimated to be 48.0 kDa by SDS-PAGE and its pI vague was 4.4. The maximum activity of the PhaZ/ sub Emi/ was observed at pH 9.0 and 55$\^{C}$. It was significantly inactivated by 1mM dithiothreitol, 2mM diisopropyl fluorphosphate, 0.1mM Tween 80, and 0.1 mM Triton X-l00, but insensitive to phenylmethylsulfonyl fluoride and N-ethylmaleimide. The PhaZ/ sub Emi/ efficiently hydrolyzed PHB and its copolyester with 30 mol% 3-hydroxyvalerate, but did not act on poly(3-hydroxyoctanoate). It also hydrolyzed p-nitrophenylacetate and p-nitrophenylbutyrate but hardly affected the longer-chain forms. The main hydrolysis product of PHB was identified as a dimer of 3-hydroxybutyrate.

• Journal of Astronomy and Space Sciences
• /
• v.1 no.1
• /
• pp.41-53
• /
• 1984

Photoelectric observations of the close eclipsing binary system CW Cep, which is well known of its apsidal motion, were made on 20 nights during August and November in 1983 using two 61cm reflectors at the Sobaeksan Observing Station of Korean National Astronomical Observatory and I1san Observing Station of Yonsei University Observatory. Standardized new light curves in UBV system are presented with a total of 1,422 individual observations. For the corrections of regional and instrumental differences, same standard stars were observed at the two observatories. Four new times of minimum light were determined with the method of Kwee and van Woerden(1956). With all of the collected times of minima, apsidal motion of this system was checked, but the O-C values calculated by the light elements of Nha(1975) and Soderhjelm (1976) did not coincide well with new times of minima. New light elements which satisfy most times of minima better, and are deduced the apsidal period derived by the new light elements turns out to be 43 years, somewhat longer than those of values previously known.

• Publications of The Korean Astronomical Society
• /
• v.30 no.2
• /
• pp.205-209
• /
• 2015

The Eclipsing Binaries Minima (BIMA) Monitoring Project is a CCD-based photometric observational program initiated by Bosscha Observatory - Lembang, Indonesia in June 2012. Since December 2012 the National Astronomical Research Institute of Thailand (NARIT) has joined the BIMA Project as the main partner. This project aims to build an open-database of eclipsing binary minima and to establish the orbital period of each system and its variations. The project is conducted on the basis of multisite monitoring observations of eclipsing binaries with magnitudes less than 19 mag. Differential photometry methods have been applied throughout the observations. Data reduction was performed using IRAF. The observations were carried out in BVRI bands using three different small telescopes situated in Indonesia, Thailand, and Chile. Computer programs have been developed for calculating the time of minima. To date, more than 140 eclipsing binaries have been observed. From them 71 minima have been determined. We present and discuss the O-C diagrams for some eclipsing binary systems.

• Proceedings of the Korean Biophysical Society Conference
• /
• 2003.06a
• /
• pp.75-75
• /
• 2003

We report here the results on N-acetyl-N'-dimethylamide of proline (Ac-Pro-NM $e_2$) calculated using the ab initio molecular orbital method with the self-consistent reaction field (SCRF) theory at the HF level with the 6-31+G(d) basis set to investigate the conformational preference of polyproline depending on the cis/trans peptide bonds and down/up puckerings along the backbone torsion angle $\square$ in the gas phase, chloroform, and water. In the gas phase, Ac-Pro-NM $e_2$ has seven local minima of tFd, tFu, cFd, cFu, cAu, tAu, and cAd conformations. In particular, polyproline conformations tFd, tFu, cFd, and cFu are found to be more stable than $\square$-helical conformations cAu, tAu, and cAd. In contrast, Ac-Pro-NHMe has seven local minima of tCd, tCu, cBd, cAu, tAu, cFd, and cFu conformations. Conformations tCd and tCu are found to be most stable, which is ascribed to the intramolecular hydrogen bond between C=O of acetyl group and $N^{~}$ H of N'-methyl amide group. The stability of the cFd conformation (i.e., the polyproline I structure) in chloroform is somewhat increased, relative to that in water, although tFd and tFu conformations (i.e., the polyproline II structure) are dominate both in chloroform and water. The population of backbone conformations feasible in chloroform and water is consistent with the experiments. This work is supported by a Korea Research Foundation Grant (KRF-2002-041-C00129).

• Journal of the Korean Society for Aviation and Aeronautics
• /
• v.20 no.4
• /
• pp.70-75
• /
• 2012

This paper is part of the research about the selection and justification of Jeju international airport as an aircraft category 'F' alternative airport, which is the results of an weather analysis based on meteorological statistics. As the results of the study, it has been only 1 day per year (58minutes) that weather conditions of Incheon and Jeju international airports, from January 2006 to June 2011, are below landing weather minimums of each airport at the same times. This means that these airports are not within the same meteorological region, which is against the current orthodoxy. In addition, it is very rare that weather conditions of Jeju international airports are below landing weather minimums when Incheon international airport is below landing weather minima. It shows that the meteorological characteristics of these regions differ widely and the designation of Jeju international airport as an alternative airport is scientifically reasonable.

• Proceedings of the KIEE Conference
• /
• 2003.11b
• /
• pp.3-6
• /
• 2003

뱀형 로봇은 일반적인 바퀴형 이동로봇과 운동 메카니즘이 상이하며 다관절로 이루어져 있기 때문에 장애물 회피에 있어 빠른 정보의 처리와 이를 위한 특별한 정보가 요구된다. 이를 실현하기 위하여 로봇은 자신의 위치를 지속적으로 파악하면서 장애물의 좌표 값과 일정한 거리의 간격을 두고 움직여야 한다. 주행 궤도 및 장애물 회피를 위한 알고리즘을 검증하기 위하여 가상 뱀형 시뮬레이터를 제작하였다. 시뮬레이터는 이동 주행 궤도를 생성하고, 지나온 궤도를 재현할 수 있는 재현기(Back Tracker), 앞으로 이루어질 뱀형 로봇의 위치와 자세를 알아보는 예견기(Predictor)로 구성된다. 시뮬레이터를 통하여 주위의 장애물을 안전하게 통과할 수 있는 일반적인 알고리즘인 포텐셜함수의 특성을 알아보고, 국소 최소점(Local Minima)에 빠지기 쉬운 단점을 극복하기 위한 방안을 제시한다. 본 논문에서는 뱀의 이동 주행 궤적을 알아보고, 주위의 장애물을 안전하게 통과할 수 있도록 하는 알고리즘에 대한 고찰과 제안한 알고리즘을 소프트웨어적인 3D 시뮬레이션을 통하여 걸과를 분석하고 검증한다.

• Journal of the Korean Institute of Telematics and Electronics C
• /
• v.35C no.3
• /
• pp.79-89
• /
• 1998

The Error Back-Propagation(EBP) algorithm is widely applied to train a multi-layer perceptron, which is a neural network model frequently used to solve complex problems such as pattern recognition, adaptive control, and global optimization. However, the EBP is basically a gradient descent method, which may get stuck in a local minimum, leading to failure in finding the globally optimal solution. Moreover, a multi-layer perceptron suffers from locking a systematic determination of the network structure appropriate for a given problem. It is usually the case to determine the number of hidden nodes by trial and error. In this paper, we propose a new algorithm to efficiently train a multi-layer perceptron. OUr algorithm uses stochastic perturbation in the weight space to effectively escape from local minima in multi-layer perceptron learning. Stochastic perturbation probabilistically re-initializes weights associated with hidden nodes to escape a local minimum if the probabilistically re-initializes weights associated with hidden nodes to escape a local minimum if the EGP learning gets stuck to it. Addition of new hidden nodes also can be viewed asa special case of stochastic perturbation. Using stochastic perturbation we can solve the local minima problem and the network structure design in a unified way. The results of our experiments with several benchmark test problems including theparity problem, the two-spirals problem, andthe credit-screening data show that our algorithm is very efficient.