• IMMUNE NETWORK
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• 제2권2호
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• pp.91-95
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• 2002

Background: In order to characterize human antibodies with specificity for mite allergens at the molecular level, a scFv phage display library was constructed using peripheral blood mononuclear lymphocytes from an asthma patient allergic to mite as Ig gene sources. Methods: Immunoglobulin $V_H$ and V gene fragments were obtained by polymerase chain reaction, and randomly combined in pCANTAB-5E vector. The resulting human scFv phage display library had $3{\times}10^4$ independent clones, and biopanning was performed with house dust mite extracts. Results: Four scFv clones specific for house dust mite extract were isolated. Immunoblot assay showed that our clones reacted to 25 kDa and 50~60 kDa proteins with unknown identity in mite extracts. Sequence analysis indicated that two clones (b7 and c15) are identical, and all clones belong to human $V_H3$ subgroup. On the other hand, light chain usage was different in that two clones (a2 and b7 / c15) belonging to V ${\kappa}4$ subgroup, but a4 used V ${\kappa}1$ light chain gene. Conclusion: Our approach should facilitate provision of useful information on the antibody responses against allergens at the molecular level in humans.

• Journal of Microbiology and Biotechnology
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• 제24권9호
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• pp.1196-1206
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• 2014

A metagenomic fosmid library was constructed using genomic DNA isolated from the gut microflora of Hermetia illucens, a black soldier fly. A cellulase-positive clone, with the CS10 gene, was identified by extensive Congo-red overlay screenings for cellulase activity from the fosmid library of 92,000 clones. The CS10 gene was composed of a 996 bp DNA sequence encoding the mature protein of 331 amino acids. The deduced amino acids of CS10 showed 72% sequence identity with the glycosyl hydrolase family 5 gene of Dysgonomonas mossii, displaying no significant sequence homology to already known cellulases. The purified CS10 protein presented a single band of cellulase activity with a molecular mass of approximately 40 kDa on the SDS-PAGE gel and zymogram. The purified CS10 protein exhibited optimal activity at $50^{\circ}C$ and pH 7.0, and the thermostability and pH stability of CS10 were preserved at the ranges of $20{\sim}50^{\circ}C$ and pH 4.0~10.0. CS10 exhibited little loss of cellulase activity against various chemical reagents such as 10% polar organic solvents, 1% non-ionic detergents, and 0.5 M denaturing agents. Moreover, the substrate specificity and the product patterns by thin-layer chromatography suggested that CS10 is an endo-${\beta}$-1,4-glucanase. From these biochemical properties of CS10, it is expected that the enzyme has the potential for application in industrial processes.

• 한국도서관정보학회지
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• 제4권
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• pp.1-38
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• 1977

This paper intend to analyze, review and point out the actual conditions of "Reserve Book System" of university libraries in Korea, then to present several proposals. I took six national universities and six private universities as researching objects and the materials were collected by questionnaires. 1) There are so many libraries which have no seperate reserve books room, while every colleges, and their departments are putting into forces the reserve book system. This makes many unresonable problems. 2) Many volumes were not adequate to the courses of each depertment and so many problems arose to set up reserve books owing to Lhe lack of library budget. 3) Insincerities of professors and librarians make no good conditions for the reserve book system. 4) For insufficiency of understanding about the reserve book system cf professors and students with university authorities, we must persuade and guide them about this system. 5) We imust evaluate about the problems of the reserve book system. I show the methods which is necessary to solve the problems of this system in the following. 1) As to methods of improving the field of menagement for running about the reserve book system, a) Establishment of the independent reserve books room. b) Gradual expansion of the reserve book system. c) Evaluation of the actual using of reserve books. 2) As to contents to improve the reserve book system, a) Improving the selection method of reserve books. b) Increasing the library budget enable to purchase more reserve books. c) Making more close connections between the curricula ard reserve books. 3) Settlment of coordinating system for carrying out reserve book system, a) We must have mutual understanding and co-operation among university authorities, faculties and libraries. b) We must have co-operation between professors and librariars.ibrariars.

• Bulletin of the Korean Chemical Society
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• 제35권8호
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• pp.2385-2390
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• 2014

Novel 2,6-difuctionalized 2H-benzopyrans were synthesized and evaluated for a sphingosylphosphorylcholine(SPC) inhibitor. The synthetic 2H-benzopyrans 1c and 3a showed high potency in SPC-induced cell proliferation assay ($IC_{50}$ < 20 nM). Neither hERG $K^+$ channel binding (> $10{\mu}M$) nor CYP inhibitions (> $10{\mu}M$) were observed. Also, the simple structure-activity relationship (SAR) results were obtained from analysis of 2H-benzopyran derivatives 1-3 and the anti-SPC effect of 2H-benzopyran 1c was confirmed by a HUVEC tube formation assay.

• 한국도서관정보학회지
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• 제50권1호
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• pp.105-124
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• 2019

본 연구는 어린이도서 분류를 위한 KDC 6판의 개선 및 적용 방안을 마련하고자 문헌연구 및 설문조사를 실시하였다. 첫째, 어린이도서의 KDC 간략화 및 세분전개 방안으로 서울시교육청 20개 도서관 및 대표적인 C 도서관이 소장한 어린이도서 주제별 통계 분포를 바탕으로 중분류, 소분류로 간략화 할 분야를 제시하고, 세분화 전개가 필요한 항목은 도서관별 분류지침을 적용하여 확장할 것을 제안하였다. 둘째, 지식그림책과 동화는 내용과 주제에 따라 각 해당항목에 분류하고, 각 나라의 동화는 세목 추가 및 장르 구분을 추가하여 특정 기호에 편중된 자료를 분산시킬 것을 제시하였다. 셋째, 이용자를 위해 연령별, 독서수준별에 따른 배가 방안과 이를 위한 가이드라인의 배포, 분류와 관련한 이용자 교육 실시를 제안하였다. 본 연구는 향후 KDC 6판 개정 시 어린이도서의 간략판 개발 마련에 기여할 수 있을 것이다.

• Nuclear Engineering and Technology
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• 제54권9호
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• pp.3440-3447
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• 2022

The accuracy of the photoatomic cross-section data is of great importance in the field of radiation protection, particularly in the characterization of radiation shielding materials. With the release of the latest and probably the most accurate photoatomic data library, EPDL2017, the need to re-evaluate all the existing and already established mass attenuation coefficients (MACs) of all radiation shielding materials arises. The MACs of several polymers, alloy-based, glasses, and building materials used in a nuclear medicine facility were investigated using the EPDL2017 library embedded in EpiXS software and were compared to MACs available in the literature. The relative differences between MAC_EpiXS and MACXCOM were negligible, ranging from 0.02% to 0.36% for most materials. However, for material like a glass comprising of elements Te and La evaluated near their corresponding K-edge energies, the relative differences in MACs increased up to 1.46%. On the other hand, a comparison with MACs calculated based on EPDL97 (a predecessor of EPDL2017) revealed as much as a 6.61% difference. Also, it would seem that the changes in MACs were more evident in the materials composed of high atomic number elements evaluated at x-ray energies compared to materials composed of low atomic number elements evaluated at gamma-ray energies.

• 정보관리학회지
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• 제40권3호
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• pp.55-76
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• 2023

본 연구는 대학도서관의 주제가이드 개발 및 개선을 위한 고려사항을 도출하기 위해 대학도서관의 주 이용자인 대학생을 중심으로 학술정보 탐색행태를 분석하였다. 분석 결과, 대학생들은 자신의 주관적 정보탐색능력 수준을 높게 평가하고 있었으나, 구체적인 검색어의 설정을 어려워하는 것으로 나타났다. 학술정보 이용 목적은 구체적이며, 하나의 데이터베이스에서 모든 정보탐색행위를 수행하고자 하는 경향을 보이고 있다. 또한 정보자원 선택 시 신뢰성, 적합성, 최신성을 주로 고려하고 있으며, 대학도서관 및 주제가이드에 대한 인식은 전반적으로 낮게 나타났으나 이에 대한 신뢰성은 높은 것으로 분석되었다. 이를 기반으로 향후 대학도서관에서 주제가이드를 개발하거나 개선할 때는 구체적인 정보탐색 목적에 따른 정보원 분류, 정보자원의 유형별 구성, 정보자원 선택 기준 관련 설명 요소 기술, 종합 데이터베이스에 대한 안내, 주제 키워드 추천, 도서관 마케팅 및 내부 기관과의 긴밀한 협업 관계를 고려하는 것이 필요하다.

• Journal of Microbiology and Biotechnology
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• 제15권5호
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• pp.1067-1072
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• 2005

A metagenomic library was constructed using a fosmid vector, and total genomic DNA was extracted directly from soil at Cisolok (hot spring area, Indonesia). This library was composed of 10,214 clones and screened for lipolytic enzyme on tributyrin agar plates. An esterase gene (estMa) was subcloned and sequenced from a positive lipolytic active clone. Esterase EstMa was encoded by a 954-bp open reading frame and showed low ($11-33\%$) amino acid similarity to known esterases. The amino acid sequence analysis demonstrated that the enzyme is a new member of lipolytic enzyme family VI. The estMa gene encodes a preprotein of 317 amino acids with a predicted molecular mass of 34,799 Da. The purified enzyme exhibited optimal activity at $50^{\circ}C$ and pH 6.5. The $K_m,\;and\;V_{max}$ values of EstMa for the hydrolysis of p-nitrophenyl valerate were $45.3\;{\mu}M$ and 4.45 U/mg, respectively.

• Journal of Information Science Theory and Practice
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• 제9권4호
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• pp.75-83
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• 2021

Mendeley reader count is good evidence of the early impact of scientific output since it appears before citations. This paper aims to scope and compare Mendeley readers of Library and Information Science (LIS) articles published from India and China. Mendeley readership data for the highly cited 1,000 articles in Web of Science are extracted using Webometric Analyst for both countries and are analysed using Excel and SPSS. The findings reveal that LIS articles that are published from China got more readers as compared to LIS articles published from India with an excess of 97 readers per paper on Mendeley. The occupational status of readers tells that PhD students are the top readers for both the countries' publications, followed by masters students. Discipline-wise readership shows that readers were spread across 29 different fields, with the highest readers from business, management and accounting, followed by computer science for both countries' publications. Location-wise readership depicts that the top engaged readers are from the United States for both the countries' publications. Finally, the study reports a positive association between citations and Mendeley bookmarks, justifying that Mendeley readership can be used to measure the early research impact of LIS scholarship in both countries.

• 대한치과의사협회지
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• 제39권12호통권391호
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• pp.1068-1080
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• 2001