• Journal of Acupuncture Research
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• v.19 no.2
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• pp.177-188
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• 2002

Objective : The purpose of this study is to investigate the immunohistological effect of Buthus martensi Karsch Herbal-acupuncture in treating the arthritis, performed several experimental items : those are paw edema, $IL-1{\beta}$, IL-6, IL-8, $TNF-{\alpha}$ and $PGE_2$. Methods : All the male Sprague Dawley rats used in this study were bred and maintaned in our pathogen-free rat colony and were 8 weeks of age at the start of to experiment. The experimental model of arthritis was induced by injection of $50{\mu}g/{\mu}{\ell}$ adjuvant(mineral oil mixed Mycobacterium butyricum). Buthus martensi Karsch Herbal-acupuncture was injected into ST36(足三里) of rats daily for 21 days. Immunohistological analysis was carried out to assess paw edema, $IL-1{\beta}$, IL-6, IL-8, $TNF-{\alpha}$ and $PGE_2$ expression in synovial membrane and sera Buthus martensi Karsch Herbal-acupuncture injected. Results : Buthus martensi Karsch Herbal-acupuncture group showed a decrease with statistical significance, in paw edema, $IL-1{\beta}$, IL-6, IL-8, $TNF-{\alpha}$ and $PGE_2$ in synovial membrane and sera compared with control group. Conclusion : Buthus martensi Karsch Herbal-acupuncture stimulation inhibited the development of immunity to adjuvant induced arthritis in rats. Thus, Buthus martensi Karsch Herbal-acupuncture may have preventive effects on autoimmune inflammatory joint diseases as arthritis. The effect of Buthus martensi Karsch Herbal-acupuncture on the immune function and the disease activity in patients with arthritis warrants further investigation.

• Journal of Acupuncture Research
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• v.23 no.2
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• pp.61-72
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• 2006

Objectives : Human chondrocytes co-treated with Buthus martensi Karsch herbal acupuncture solution(BMK-HAS) extract produced significantly less NO compared with chondrocytes stimulated with $IL-1{\beta}$ alone Methods : Activation and translocation of and NF-kB DNA binding activity were determined by Western blotting and specific enzyme-linked immunosorbent assay. Results : The inhibition of NO production correlated with the suppression of induction and expression of nuclear factor-kB (NF-kB) and activation protein-1 (AP-1)-dependent gene. BMK-HAS inhibited the activation and translocation of NF-kB to the nucleus, indicating that BMK-HAS inhibits the $IL-1{\beta}-induced$ production of NO in human chondrocytes by interfering with the activation of NF-kB through a novel mechanism. In addition, BMK-HAS reduced prostaglandin E2 (PGE2)production in mouse peritoneal macrophages stimulated with lipopolysaccharide, whereas no influence on the activity of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2) or cyclooxygenase-1 (COX-1) was observed. My data, therefore, suggest that BMK-HAS may be a therapeutically effective inhibitor of $IL-1{\beta}-induced$ inflammatory effects that are dependent on NF-kB activation in human OA chondrocytes. Conclusion : The results indicate that BMK-HAS exerts anti-inflammatory effects related to the inhibition of neutrophil functions and of NO and PGE2 production, which could be due to a decreased expression of iNOS and COX-2 through the transcription factors NF-kB and AP-1.

• Journal of Acupuncture Research
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• v.20 no.1
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• pp.104-119
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• 2003

목적 : 면역억제 작용을 지닌 것으로 알려진 전갈약침(BMK)의 IL-1으로 야기된 1차성 골관절염 인체 연골 세포에 대한 항염증 효과 골 기능 효과에 대해 연구하였다. 방법 : 골관절염 연골에서 채취된 인체 연골세포는 ID-1(2ng/ml)에 의해 처리되어졌으며, IL-1과 BMK($10{\mu}g/ml$)를 함께 처리한 연골세포와 비교하였다. 결과 : IL-1 단독처리된 연골세포에 비해 BMK가 함께 처리된 연골세포에서 연골세포의 손실과 퇴화의 중요한 요소인 NO의 생산량이 의미있게 저하되었다. IL-1단독으로 처리된 연골세포보다 IL-1과 BMK가 함께 처리된 연골세포에서 iNOS mRNA의 단백질 합성이 의미있게 감소하였다. 또한, 전사인자로서의 NF-B의 활성화가 IL-1 단독으로 처리된 연골세포에 비하여 BMK가 함께 처리된 군에서 상대적으로 의미있게 억제되었다. 결론: 이상의 결과를 종합하면 BMK가 인제 골관절염 연골에 있어서 NF-B 활성화에 의존한 IL-1 유도염증의 치료상에 효과적인 반응억제제임을 시사하며, 골 세포의 골 재흡수 활동에 효과적임을 시사한다.