• Korean Journal of Weed Science
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• v.5 no.2
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• pp.131-136
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• 1985

This experiment was conducted to identify the competitive ability of rice against Monochoria vaginalis Presl. and to determine effect of various herbicides on it. Photosynthetic efficiency of rice markedly increased as the density of M. vaginalis increased from 1 to 3 plants per hill. Competition index increased in propotional to an increase of M. vaginalis density while total dry matter of rice decreased in reverse. Significant yield reduction of rice, was observed at the density of M. vaginalis, 2 plants per hill and 37% at 3 plants per hill. Such a yield reduction can be mainly attributed to the decrease of panicle and spikelet number of rice which were greatly affected by competition with M. vaginalis. Regardless of herbicides tested, % inhibition increased remarkably as the concentration of herbicides increased from 1 to 20 ppm. No plant growth was observed in all the herbicides treated with 20 ppm except for butachlor, thiobencarb and bifenox, showing existence of the new promising herbicides to control M. vaginalis among the herbicides tested.

• Weed & Turfgrass Science
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• v.6 no.1
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• pp.32-39
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• 2017

This study was carried out to investigate the ACCase inhibiting herbicide resistant Echinochloa oryzicola collected in a Honam rice field and to investigate the control of E. oryzicola in directly seeded paddy rice field. The study result showed that about 44% of the 67 biotypes collected in the rice field in Honam region were resistant to ACCase-inhibiting herbicides. The efficacy of several chemical herbicides on these resistant E. oryzicola biotypes was evaluated according to their application timings. Among herbicides applied 4 days before seeding, pretilachlor, butachlor, oxadiazon, oxadiargyl, oxadiazon+pretilachlor, and fentrazamide+oxadiargyl, provided 95% or higher control of the resistant E. oryzicola biotypes. Among the herbicides applied 10 days after seeding, bromobutide+fentrazamide+imazosulfuron, benzobicyclon+fentrazamide+imazosulfuron, pyrazosulfuron-ethyl+thiobencarb, fentrazamide+imazosulfuron, bromobutide+imazosulfuron+mefenacet and bromobutide+imazosulfuron+pyraclonil provided ${\geq}95%$ control of the weeds. Bromobutide+imazosulfuron+mefenacet and bromobutide+pyrazosulfuron-ethyl+pyriminobac-methyl, applied 15 days after seeding, provided ${\geq}95$ and 90% control of the weeds. Foliar application of cyhalofop-butyl+propanil at 30 days after seeding provided ${\geq}90%$ control of the weeds. In addition, oxaziclomefone provided ${\geq}90%$ control for 40 days after soil application. These results show that E. oryzicola biotypes resistant to ACCase-inhibiting herbicides could be effectively controlled in directly seeded rice in submerged paddy fields using single or tank-mixed chemical herbicides currently available on the market.

• Korean Journal of Soil Science and Fertilizer
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• v.34 no.3
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• pp.185-191
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• 2001

To obtain the basic information for degradation of remaining pesticide accumulated in cultivated soil of Honam area, the resistant bacterial strains were investigated in Chlorothalonil(TPN). Mancozeb, Bentazone, and Butachlor levels of 100, $500{\mu}g\;ml^{-1}$, and degradation of TPN by TPN-resistant bacteria in sterilized soil was studied under TPN levels 0, 10, 50 and $100{\mu}g\;g^{-1}$. A number of resistance strains were decreased with higher at concentration level of pesticide, and were higher in greenhouse than upland or paddy soil. The resistance of bacteria was strong in other of Bentazone> Butachlor> TPN> Mancozeb. The percentage of bacterial strains of resistance for pesticides isolated from the cultivated soil were the highest in Acinetobacter spp. and Corynebacterium spp., and the lowest in Moraxella spp. A number of TPN-resistant strains were the highest at the TPN level of $10{\mu}g\;g^{-1}$, and 5 days after strains inoculation, and were higher in Pseudomonas spp. TD-25 than TC-23 or strains in non-sterilized soil. The degradation of TPN was fast in order of strain TD-25>strain in non-sterilized soil >TC-23.

• Weed & Turfgrass Science
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• v.2 no.4
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• pp.352-357
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• 2013

A survey was performed to investigate major paddy field weeds and frequently used herbicides in Gyeonggi province, Korea in order to establish a system to efficiently control herbicide-resistant weeds. The dominant paddy field weeds included Echinochloa spp. (22%), Eleocharis kuroguwai (14%), Sagittaria trifolia (13%), Scirpus juncoides (12%), Monochoria vaginalis (9%) and Sagittaria pygmaea (7%), and the most widely used herbicides were butachlor 33% CS (25%), benzobicyclon + fentrazamide + imazosulfuron 11.5% SC (9%), cyclosulfamuron + mefenacet 22.2% SC (9%), butachlor 5% GR (6%), and oxadiazon 12% EC (6%) in Gyeonggi province, Korea. Many paddy rice growers considered S. juncoides, M. vaginalis, S. pygmaea and C. difformis as herbicide-resistant weeds. An extensive research deserves to be conducted to monitor occurrence of herbicideresistant paddy weeds in Gyeonggi province, Korea.

• Korean Journal of Environmental Agriculture
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• v.13 no.1
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• pp.60-65
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• 1994

Absorption and translocation of bensulfuron-methyl {methyl 2[[[[[(4,6-dimethoxy-2-pyrimidinyl) amino]carbonyl]amino]sulfonyl]methyl]benzoate} in rice (Oryza sativa L.) as affected by mineral nutrients and mixed herbicides were determined using the $^{14}C-labeled$ herbicide in culture solution. Absorption of $^{14}C-bensulfuron-methyl$ by the root decreased with increasing concentration of bensulfuron-methyl. However, increase in the application concentration did not affect movement of the $^{14}C$ to the shoot. There was no difference in total amount of $^{14}C-bensulfuron-methyl$ taken up between absorption periods of 12 and 48 hours, whereas $^{14}C-bensulfuron-methyl$ translocated to the shoot increased with increasing the absorption period. When bensulfuron-methyl mixtures were applied, butachlor [N-(butoxymethyl)-2-chloro-N-(2',6'-diethylphenyl)acetamide] did not affect absorption and translocation of $^{14}C-bensulfuron-methyl$. However, quinclorac (3,7-dichloro-8-quinoline carboxylic acid) mixed at a high concentration resulted in decrease in absorption and translocation of $^{14}C-bensulfuron-methyl$. Nutritional disorder such as deficient or excess supply of mineral nutrients caused to inhibit absorption of $^{14}C-bensulfuron-methyl$. The greatest decrease and delay of $^{14}C-bensulfuron-methyl$ absorption and/or translocation occurred in N-deficient and S-excess supply conditions.

• The Korean Journal of Pesticide Science
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• v.16 no.2
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• pp.137-144
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• 2012

In order to monitor the pesticide residues in rice paddy soils and paddy waters in Korea and evaluate leachabilities to groundwater of the pesticides detected, paddy soils and paddy waters were collected twice from 40 sites of rice growing paddy fields in July and August in 2011, respectively. Pesticide residues in the samples were analyzed by multi-residue method with GC and HPLC. Five pesticides, such as butachlor, cypermethrin, iprobenfos, oxadiazon, and pendimethalin were detected from thirteen paddy soils and detection rate was about 16.3%, while no pesticide was detected in paddy water. As a results of the estimation of leachabilities for the pesticides detected using GUS (groundwater ubiquity score), their GUSs were less than 1.3, representing they have no potentials to leach into groundwater.

• Korean Journal of Weed Science
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• v.10 no.4
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• pp.285-293
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• 1990

The response of callus growth of rice (Oryza sativa L.) cultivars which showed different responses to herbicides as seedlings was investigated to select resistant or tolerant calli. Callus growth of IR28 which was susceptible to thiobencarb (S-[(4-chlorophenyl)methyl]diethylcarbamothioate) during callus induction was not inhibited by $10^{-5}$ M and $10^{-6}$ M thiobencarb, indicating that there was a difference in tolerance among callus induction and growth, and the intact plant level. A similar result was obtained with IR31917-45-3-2-2 to butachlor [N - (buthoxymethyl) -2-chloro-N- (2, 6-diethylphenyl) acetamide]. The fresh weight of IR28 callus transferred into $10^{-5}$ M thiobencarb after treatment at $10^{-6}$ M for 30 days was not affected by the herbicide, indicating that transferring callus into gradually higher herbicide concentrations can be a useful method for selection of herbicide-tolerant cell lines.

• Applied Biological Chemistry
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• v.24 no.3
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• pp.174-180
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• 1981

As the amount of pesticides consumption increases in agriculture, the side effects of pesticides on soil microorganisms have become an essential part in safety evaluation of pesticide for continued soil fertility. In order to establish the method of safety assessment of pesticides in Korea, a series of tests were carried out. Among the paddy pesticides Fujione (Isoprothiolane, fungicide), Ortran (Acephate, insecticide), and Machete (Butachlor, herbicide) were chosen and the effects of above three pesticides on Korean paddy soil microorganisms were studied. The measurements of pesticide effects on the cycling of carbon ($CO_2$ production), nitrogen fixation ($C_2H_2$ reduction), nitrification, and dehydrogenase activity were carried out. These measurements were complemented by evaluation of pesticide effects on viable microbial numbers. Although Fujione reduced the fungi numbers and inhibited nitrogen cycling activities somewhat, no significant adverse effects were show by any of the tested pesticides in our study.

• Korean Journal of Plant Tissue Culture
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• v.26 no.3
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• pp.183-187
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• 1999

To establish an efficient screening system for new herbicides using plant cultured cells, responses of tobacco photomixotrophic cultured (PH) cells to various herbicides with different modes of action were surveyed by measuring the cell growth and ion conductivity in medium. The cells were cultured in Murashige and Skoog (MS) medium containing 0.7mg/L 2,4-D, 0.3mg/L kinetin and 30 g/L sucrose at $25^{\circ}C$ in the light (100 rpm). Chemicals were treated to suspension cultures of tobacco PH cells at the time of subculture. The cell growth and ion conductivity in the medium were investigated on 12 days after chemical treatment. The ion conductivity assay gave well correlated results to the cell growth inhibition data. The responses of tobacco PM cells were dependent on the modes of action of chemicals tested. Atrazine, an inhibitor of photosynthetic electron transport (PET), strongly inhibited both the cell membrane and cell growth ($IC_{50}$/, about 1 $\mu$M). Butachlor (an inhibitor of cell division), glufosinate (an inhibitor of amino acid biosynthesis), and fluridone (an inhibitor of carotenoid biosynthesis) showed a dose-dependent inhibition. However, Quinclorac, a herbicide with an auxin activity, did not affect the cell growth and ion leakage. These results suggested that tobacco PM cells is suitable materials for the simple screening of new herbicides such as PET, amino acid biosynthesis, ceil division inhibitors by measuring the cell growth and ion conductivity.

• Korean Journal of Environmental Agriculture
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• v.2 no.2
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• pp.108-113
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• 1983

There has been two kind of research fields in evaluating the ecotoxicity of chemicals The one is a bioassay and the other is an ecosystem analysis. The toxicants are transported into different biota, which have quite different environmental behaviour patterns and toxic properties. The effects of pesticides (butachlor, carbofuran, and tricyclazole) on periphyton community was studied by analyzing content of chlorophyll-a and autotrophic index (AI) that is a means of determining the trophic nature of the periphyton community. Results indicated that the content of chlorophyll-a was not influenced by the pesticides. The growth of algae was inhibited by sunshine period. And AI value is 43-2027: the large value indicates heterotrophic periphyton community, and was not affected by pesticides.