• Korean Journal of Soil Science and Fertilizer
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• v.29 no.1
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• pp.20-25
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• 1996

This survey was conducted to investigate the chemical properties of soils cultivated medicinal plants. The soils were collected at 254 sites(Angelica gigas : 81, Astragalus membranceus : 38, Platycodn glandiflorum : 36, Paeonia albiflora : 34, Codonopsis lanoceolata : 32, Ligusticum chuanxiong : 17. Bupleurum falcatum : 16, respectively) throughout the country by 0-15cm depth. The chemical properties of soils were pH 5.6, O.M 3.0%. Av.$P_2O_5$ : 405 mg/kg, Ex.K : $0.67cmol^+/kg$, Ex.Ca : $6.3cmol^+/kg$, Ex.mg : $1.6cmol^+/kg$. The percentage distribution of pH on the basis of soil sample numbers were much more at the 5.1-6.0 range than any other ranges, especially those of below pH 6.0 were 83-91% for Angelica gigas, Platycodn glandiflorum and Codonopsis lanoceolata. The distribution of OM and Av.$P_2O_5$ in soils were much at the 2.1-4.0% and above 500mg/kg ranges, respectively. And the content of those in soils cultivated with Platycodn glandiflorum were lower than any other medicinal plants. The distribution of exchangeable cations in soils were much at the 0.2-0.8, 2.1-4.0 and $0.5-2.0cmol^+/kg$ of Ex.K. Ca and mg. respectively, and the contents were higher in soils cultivated Astragalus membranceus. and lower in soils cultivated Platycodn glandiflorum and Codonopsis lanoceolata than any other samples.

• Korean Journal of Plant Resources
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• v.24 no.5
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• pp.549-556
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• 2011

We investigated the plant phenology on the threatened species for climate change in the summit area of Mt. Deogyusan which is a representative sub-alpine zone in Korea. We had performed the monitoring survey of plant phenology on 38 species including 20 trees and 18 herbs from May 2009 to November 2010. The investigated phenological charateristics were five dates for leafing, flowering, floral abscission, autumn leaf colors and leaf abscission on each plant species in sub-alpine region. The climate data were measured from November 2009 to December 2010. The range of temperature was from 30.4 to -$20.3^{\circ}C$ at Hyangjeokbong to Jungbong region, and the relative humidity was 100% to 3.4%. The leafing dates in 2010 were similar to 2009 or were 6-20 days delayed in most of the investigated species except Veratrum oxysepalum and Sanguisorba hakusanensis which showed 8 days earlier leafing dates in 2010. The biggest difference among phenological characters was found in flowering dates. The flowering dates of early Spring blooming species such as Heloniopsis koreana, Rhododendron yedoense for. poukhanense and Viola orientalis showed 13-20 days earlier in 2010, and the several summer flowering species as Viburnum opulus var. calvescens, Smilacina japonica and Bupleurum longeradiatum showed 6-10 days delay in 2010. The dates for floral abscission and autumn leaf colors in 2010 were delayed about 10-18 days, and leaf abscission dates were similar to 2009. The effects of climate change on the phenology for the threatened species in sub-alpine zones of Korea are occuring especially on flowering, floral abscission and autumn leaf colors.

• Korean Journal of Plant Resources
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• v.20 no.2
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• pp.201-211
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• 2007

The vascular plants at the nature every second year in Wolchulsan National Park was listed 325 taxa: 86 families, 205 genera, 283 species, 1 subspecies, 36 varieties and 5 forms. Based on the list of the rare plants by the Forest Research Institute, 3 taxa were recorded in the studied areas; Lilium callosum (Preservation priority order; No. 191), Viola albida (No. 202), Wikstroemia trichotoma (No. 120) and based on the list of Korean endemic plants, 8 taxa were recorded; Carex okamotoi, Lilium amabile, Carpinus coreana, Clematis trichotoma, Stewartia koreana, Ajuga spectabilis, Weigela subsessilis, Adenophora triphylla var. hirsute. Specific plants by floral region were total 44 taxa; Prunus davidiana, Wistaria floribunda in class IV, 9 taxa (Neolitsea aciculata, Vaccinium bracteatum, Utricularia racemosa, etc.) in class III, 5 taxa (Bupleurum longiradiatum, Ostericum melanotilingia, Cirsium schantarense) in class II, 28 taxa (Polygonatum falcatum, Eurya japonica, Ajuga spectabilis, etc.) in class I. The naturalized plants in this site were 4 families, 6 genera, 9 taxa and naturalization rate was 2.8% of all 325 taxa vascular plants.

• Journal of Food Hygiene and Safety
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• v.13 no.4
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• pp.355-359
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• 1998

Platelet activation is originated by the intracellular or/and extracellular $Ca^{2+}$. Agonist-induced $Ca^{2+}$ entry through a plasma-membrane pathway has been reported repeatedly, but the mechanisms has proven harder to elucidate. Recently, a number of natural products have been isolated from medicinal plants and marine organisms and have proved to be useful chemical tools for resolving the mechanism of cellular functions. In an attempt to understand the mechanism of platelet activation in Bupleuri Radix, we have studied some aspects of the isolation of active components and their dependence of external $Ca^{2+}$> on platelet activation. Acetone extract of Bupleuri Radix has the most activity on platelet activation and it's active components were identified as saikosaponin a and d. Their optimal concentration was respectively $20\;\mu\textrm{g}/ml$ and $5\;\mu\textrm{g}/ml$ and their platelet activation was not dependent on external $Ca^{2+}$>, whereas optimal concentration of each agonist was arachidonic acid ($10\;\mu\textrm{M}$), collagen ($10\;\mu\textrm{M}/ml$), thrombin (0.1 unit/mi), PAF (5 nM), PMA ($5\;\mu\textrm{M}$), ionophore A23187 ($2\;\mu\textrm{g}$) and their dependence of external $Ca^{2+}$> on platelet activation appeared to thrombin > collagen $\geq$ PAF > PMA > arachdonic acid> ionophore A23187. These results suggest that saikosaponin is different from each agonists in the dependence of external $Ca^{2+}$ on platelet activation.

• Journal of Korean Society of Forest Science
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• v.104 no.3
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• pp.495-502
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• 2015

This study intended to provide basic data required in establishing policies for improving the forestry management and the compensation standard for the loss from non-timber forest products by examining the cost of cultivating key medicinal herbs and the earnings from them to analyze the income. According to the income analysis on the cultivation of medicinal herbs, the average annual income per unit area of Adenophora triphylla var. japonica Hara was the highest as it recorded 14,233,000 won/10a and was followed by Pleuropterus multiflorus TURCZ. which recorded 4,121,000 won/10a, Gastrodia elata Blume 3,766,000 won/10a, Epimedium koreanum Nakai 3,537,000 won/10a, Atractylodes ovata (Thunb.) DC. 2,655,000 won/10a, Aralia continentalis Kitagawat 1,048,000 won/10a, Paeonia lactiflora Pallas 1,025,000 won/10a, and Bupleurum falcatum L. 919,000 won/10a. Compared with the income from major nuts and fruits analyzed in 2014, the average annual income per unit area for medicinal herbs was relatively higher. For Adenophora triphylla var. japonica Hara and Aralia continentalis Kitagawat, soots are used for food and the roots for medicine, it appears that it can become a new income source for the farming and mountain villages. Meanwhile, the price for Paeonia lactiflora Pallas is dropping due to Chinese imports, and also damage to the income from other medicinal herbs due to Chinese imports is expected with the implementation of Korea-China FTA in the future.

• Journal of the Korean Society of Food Science and Nutrition
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• v.41 no.7
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• pp.914-920
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• 2012

We investigated the effects of heat treatment and extraction method on the antioxidant activities of five medicinal plants: Cyperus rotundus, Eucommia ulmoides, Bupleurum falcatum, Achyranthes japonica Nakai, and Akebia quinata. Extraction was performed with only ultrasound, ultrasound after heating at $130^{\circ}C$ for 2 hours, and reflux extraction with distilled water. The phenolic contents of reflux extraction and ultrasound extraction after heating were higher than only ultrasound extraction, and ultrasound extraction after heating samples was higher than reflux extraction except for Eucommia ulmoides and Cyperus rotundus. Total flavonoid content was higher in reflux and ultrasound extraction after heating samples than only ultrasound extraction, except for Cyperus rotundus. ABTS radical scavenging activity was higher in reflux extraction and ultrasound extraction after heating a sample, than only ultrasound extraction. DPPH radical scavenging activity was higher in reflux extraction except for Achyranthes japonica Nakai and Akebia quinata. The reducing power of ultrasound extraction after heating was higher with Achyranthes japonica Nakai. From the results of this study, we can expect to increase the antioxidant activity of medicinal plant extracts by applying suitable extraction and pretreatment conditions on the type of medicinal plant.

• Journal of dental hygiene science
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• v.7 no.3
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• pp.197-200
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• 2007

This study was carried out to assess anticariogenic activity from natural products. The hexane extracts of 11 medicinal plants were tested for the antimicrobial activities against a cariogenic bacterium, S. sobrinus B13. The hexane extracts of 11 medicinal plants such as Alisma orientale, Cyperus rotundus, Saussurea lappa, Atractylodes japonica, Kalopanax pictus, Angelica gigas, Salvia miltorrhiza, Lonicera japonica, Gardenia jasmoides, Aurantii nobilis percarpium and Bupleurum lorgiradiatum were used for this study, and S. sobrinus B13 was cultured with 0.5% extracts of the medicinal plants, in trypticase soy broth medium under 5% $CO_2$ for 0, 6, 12, 24 hours at $37^{\circ}C$. The obtained results were as follows. Immediately after culturing for inoculation, Kalopanax pictus and Cyperus rotundus showed better antimicrobial activities than those of others. After culturing for 6 hours, the order of effective antimicrobial activity was Salvia miltorrhiza, Angelica gigas. After incubation for 12 hours, Salvia miltorrhiza showed the best antimicrobial activity. After incubation for 24 hours Salvia miltorrhiza, Cyperus rotundus, Saussurea lappa, Atractylodes japonica had better antimicrobial activity than others. In conclusion, among Hexane extracts of 11 medicinal plants, Cyperus rotundus, Saussurea lappa, Atractylodes japonica showed effective antimicrobial activity.

• Journal of Ecology and Environment
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• v.42 no.1
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• pp.1-11
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• 2018

Background: In the study, the effects of elevated $CO_2$ and temperature on the nitrogen content, carbon content, and C:N ratio of seven rare and endangered species (Quercus gilva, Hibiscus hambo, Paliurus ramosissimus, Cicuta virosa, Bupleurum latissimum, Viola raddeana, and Iris dichotoma) were examined under control (ambient $CO_2$ + ambient temperature) and treatment (elevated $CO_2$ + elevated temperature) for 3 years (May 2008 and June 2011). Results: Elevated $CO_2$ concentration and temperature result in a decline in leaf nitrogen content for three woody species in May 2009 and June 2011, while four herb species showed different responses to each other. The nitrogen content of B. latissimum and I. dichotoma decreased under treatment in either 2009 and 2011. The leaf nitrogen content of C. virosa and V. raddeana was not significantly affected by elevated $CO_2$ and temperature in 2009, but that of C. virosa increased and that V. raddeana decreased under the treatment in 2011. In 2009, it was found that there was no difference in carbon content in the leaves of the six species except for that of P. ramosissimus. On the other hand, while there was no difference in carbon content in the leaves of Q. gilva in the control and treatment in 2011, carbon content in the leaves of the remaining six species increased due to the rise of $CO_2$ concentration and temperature. The C:N ratio in the leaf of C. virosa grown in the treatment was lower in both 2009 and 2011 than that in the control. The C:N ratio in the leaf of V. raddeana decreased by 16.4% from the previous year, but increased by 28.9% in 2011. For the other five species, C:N ratios increased both in 2009 and 2011. In 2009 and 2011, chlorophyll contents in the leaves of Q. gilva and H. hamabo were higher in the treatment than those in the control. In the case of P. ramosissimus, the ratio was higher in the treatment than that in the control in 2009, but in 2011, the result was the opposite. Among four herb species, the chlorophyll contents in the leaves of C. virosa, V. raddeana, and I. dichotoma did not show any difference between gradients in 2009, but decreased due to the rise of $CO_2$ concentration and temperature in 2011. Leaf nitrogen and carbon contents, C:N ratio, and chlorophyll contents in the leaves of seven rare and endangered species of plant were found to be influenced by the rise and duration of $CO_2$ concentration and temperature, species, and interaction among those factors. Conclusions: The findings above seem to show that long-term rise of $CO_2$ concentration, and temperature causes changes in physiological responses of rare and endangered species of plant and the responses may be species-specific. In particular, woody species seem to be more sensitive to the rise of $CO_2$ concentration and temperature than herb species.

• Food Science and Preservation
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• v.18 no.4
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• pp.620-625
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• 2011

This is a part of the study on the food materials that are effective for diabetes treatment and for use in the development of functional bread products. In this study, various commercially available Oriental medicines with the intestinal absorption enzyme called ${\alpha}$-glucosidase, which is known to be effective for diabetes treatment, were explored. According to the research results on the water and methanol in 200 kinds of Oriental medicines, which were separated by layer to investigate the inhibitory activity of ${\alpha}$-glucosidase, Astragalus membranaceus (70.9%) in the water layer and Pericaeta communissima (72.9%) in the MeOH layer showed a strong inhibitory effect of over 70%. Myristica fragrans (69%), Morus alba (66.9%), Schisandra chinensis (65%), Panax notoginsens (63.9%), Anthriscus sylvestris (62.9%), Asparagus cochinchinensis (62.1%), Erycibe obtusifolia (60.9%), Polygonum cuspidatum (60.7%), Atractylodes lancea (60.2%), and Perilla frutescens (60.2%) in the water layer, and Codonopsis pilosula (67.8%), Prunus persica batsch (67.6%), Sinomenium acutum (63.5%), and Malvae semen (61.6%) in the MeOH layer, showed a more than 60% inhibitory effect. Thirty one species, including Polygonatum sibiricum (59.8%), Medicata fementata (59.7%), Alisma canaliculatum (59.5%), Coix lacryma-jobi (59.2%), Asiasarum sieboldi (59.0%), and Bupleurum falcatum (53.0%), in the water layer, and 10 species [Quisqualis indica (58.8%), Lycium chinense (58.3%), Trichosanthes kirilowii (58.0%), Thuja orientalis (55.9%), Bombyx mori (55.6%), Gallus domesticus (55.4%), Aralia continentalis (55.3%), Cibotium barometz (52.7%), Euphorbia pekinensis (52.7%), and Dolichos lablab (52.5%)] in the MeOHlayer, showed a more than 50% inhibitory effect. Therefore, such materials are expected to be the basic materials that will be used for the development of functional materials for diabetes treatment.

• Korean Journal of Environment and Ecology
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• v.30 no.5
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• pp.803-809
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• 2016

In order to elucidate the ecological responses of B. latissimum under global warming situation and prepare the conservation measures, we cultivated the plant within control(ambient $CO_2+$ambient temperature) and treatment(elevated $CO_2+$elevated temperature) from 2010 to 2011. Rising $CO_2$ concentration was treated with 2 times and temperature increased with $2.0^{\circ}C$ above than control. As a result, Growth response has received more the effect of $CO_2+$Temperature rising than light, moisture, nutrients, and it was grow well in $CO_2+$temperature rise sphere when many nutrients, and it was grow well in $CO_2+$temperature rise sphere when many nutrients. No. of leaves were many number in the treated group compared to the control at a low light gradient and high nutrient gradient, leaf width was narrow in the treatment compared to the control in the middle gradient of light and nutrients. Shoot length, petiole length, lamina length was no difference between control and $CO_2+$temperature rise sphere. Based on the results of this study, in order to preserve the B. latissimum in future climate, we should be supply of nutrients(containing ${NH_4}^+$, $NO_3$, $P_2O_5$, $K_2O$, etc.) in the habitat of the B. latissimum, and must ensure that the habitat of B. latissimum is not destroyed. Also, We should be find similar area with habitat of the B. latissimum, restoring, expanding.