• Macromolecular Research
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• 제16권5호
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• pp.429-433
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• 2008

Alginate/carboxymethyl scleroglucan (CMSG) hydrogels were suggested as a novel carrier for the controlled release of protein drugs. The drug release characteristics of alginate hydrogels were improved by CMSG addition. Scleroglucan (Sclg) was carboxymethylated using monochloroacetic acid in aqueous alkaline medium. Alginate/CMSG hydrogels were prepared by dropping the mixture solution of alginate/CMSG into calcium chloride solution. The swelling behaviors and drug release characteristics of the hydrogels were investigated in the buffers of pH 1.2 or 7.4. As the CMSG content increased in the hydrogels, the swelling ratio of the alginate/CMSG hydrogel increased rapidly in the buffer of pH 7.4. At pH 1.2, however, the swelling ratio significantly decreased compared to that at pH 7.4. According to in vitro release tests, only 15% of ovalbumin, investigated as a model protein drug, was released from the alginate/CMSG hydrogels at pH 1.2 within 6 h. At pH 7.4, however, the drug release significantly increased due to the rapid swelling of the hydrogels. The release and swelling behaviors of the hydrogels could be controlled by changing the CMSG content in the hydrogels. These results supported the use of alginate/CMSG hydrogels as a suitable carrier for the controlled release of protein drugs in a pH responsive manner.

• The Korean Journal of Pain
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• 제27권3호
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• pp.239-245
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• 2014

Background: Neuropathic pain induced by spinal or peripheral nerve injury is very resistant to common pain killers, nerve block, and other pain management approaches. Recently, several studies using stem cells suggested a new way to control the neuropatic pain. In this study, we used the spinal nerve L5 ligation (SNL) model to investigate whether intrathecal rat mesenchymal stem cells (rMSCs) were able to decrease pain behavior, as well as the relationship between rMSCs and reactive oxygen species (ROS). Methods: Neuropathic pain of the left hind paw was induced by unilateral SNL in Sprague-Dawley rats (n = 10 in each group). Mechanical sensitivity was assessed using Von Frey filaments at 3, 7, 10, 12, 14, 17, and 24 days post-ligation. rMSCs ($10{\mu}l$, $1{\times}10^5$) or phosphate buffer saline (PBS, $10{\mu}l$) was injected intrathecally at 7 days post-ligation. Dihydroethidium (DHE), an oxidative fluorescent dye, was used to detect ROS at 24 days post-ligation. Results: Tight ligation of the L5 spinal nerve induced allodynia in the left hind paw after 3 days post-ligation. ROS expression was increased significantly (P < 0.05) in spinal dorsal horn of L5. Intrathecal rMSCs significantly (P < 0.01) alleviated the allodynia at 10 days after intrathecal injection (17 days post-ligation). Intrathecal rMSCs administration significantly (P < 0.05) reduced ROS expression in the spinal dorsal horn. Conclusions: These results suggest that rMSCs may modulate neuropathic pain generation through ROS expression after spinal nerve ligation.

• Restorative Dentistry and Endodontics
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• 제41권4호
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• pp.246-254
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• 2016

Objectives: The aim of this investigation was to give insights into the impact of carbohydrate-electrolyte drinks on the likely capacity of enamel surface dissolution and the influence of human saliva exposure as a biological protective factor. Materials and Methods: The pH, titratable acidity (TA) to pH 7.0, and buffer capacity (${\beta}$) of common beverages ingested by patients under physical activity were analyzed. Then, we randomly distributed 50 specimens of human enamel into 5 groups. Processed and natural coconut water served as controls for testing three carbohydrate-electrolyte drinks. In all specimens, we measured surface microhardness (Knoop hardness numbers) and enamel loss (profilometry, ${\mu}m$) for baseline and after simulated intake cycling exposure model. We also prepared areas of specimens to be exposed to human saliva overnight prior to the simulated intake cycling exposure. The cycles were performed by alternated immersions in beverages and artificial saliva. ANOVA two-way and Tukey HDS tests were used. Results: The range of pH, TA, and ${\beta}$ were 2.85 - 4.81, 8.33 - 46.66 mM/L and 3.48 - $10.25mM/L{\times}pH$, respectively. The highest capacity of enamel surface dissolution was found for commercially available sports drinks for all variables. Single time human saliva exposure failed to significantly promote protective effect for the acidic attack of beverages. Conclusions: In this study, carbohydrate-electrolyte drinks usually consumed during endurance training may have a greater capacity of dissolution of enamel surface depending on their physicochemical proprieties associated with pH and titratable acidity.

• 한국시뮬레이션학회논문지
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• 제22권3호
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• pp.35-41
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• 2013

본 연구는 생산 공정 간 버퍼 제약이 있는 flow shop 시스템에 활동기간 기반 애로공정 발견 기법을 적용하여 그 타당성을 분석하였다. 생산 시스템에는 보통 생산성을 저하시키는 1개 또는 1개 이상의 애로공정이 존재한다. 전통적인 애로공정을 발견하는 기준으로 공정의 대기 시간이나 대기 공정의 길이 또는 공정의 이용률이 자주 활용된다. 애로공정은 다른 공정작업을 대기 상태로 만들어 전체적으로 시스템의 생산성을 저하시키는 공정으로 공정 시간과 기계의 고장 및 수리 시간의 확률적인 특성으로 인하여 애로공정은 생산과정에서 수시로 다른 공정으로 변환된다. 어떤 공정이 언제 활동기간으로 변화하는 정보를 이용하여 애로공정을 발견하는 기법을 범용 시뮬레이션 언어 AweSim에서 구현하였다. 시뮬레이션 결과, 활동기간 기법과 단독 및 변환 애로공정 기간 비율 기법이 전통적인 기법과 비교하여 애로공정을 발견하는데 효과적인 것으로 나타났다. 간단한 flow shop 모형을 대상으로 얻은 결과이지만 복잡한 시스템에도 적용될 수 있을 것으로 기대되며 애로공정 개선을 통한 생산시스템 가용 자원의 효과적인 배치는 생산성을 향상시키는데 기여할 것으로 사료된다.

• 산업경영시스템학회지
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• 제41권3호
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• pp.120-128
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• 2018

OLED Display fabrication system is one of the most complicated discrete processing systems in the world. As the glass size grows from $550{\times}650mm$ to $1,500{\times}1,850mm$ in recent years, the efficiency of Automated Material Handling System (AMHS) has become very important and OLED glass manufacturers are trying to improve the overall efficiency of AMHS. Aiming to meet the demand for high efficiency of transportation, various kind of approaches have been applied for improving dispatching rules and facility layout, while simultaneously considering the system parameters such as glass cassettes due date, waiting time, and stocker buffer status. However, these works did not suggest the operational policy and conditions of distribution systems, especially for handling unnecessary material flows such as detour. Based on this motivation, in this paper, we proposed an efficient algorithm for improving detour transportation in OLED FAB. Specifically, we considered an OLED FAB simplifying OLED production environment in a Korean company, where four stockers are constructed for the delivery of Lot in a bay and linked to processing equipments. We developed a simulation model using Automod and performed a numerical experiment using real operational data to test the performance of three operation policies under considerations. We showed that a competitive policy for assigning alternative stocker in case of detour was superior to the current dedicated policy using a specified stocker and other considered policies.

• Archives of Pharmacal Research
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• 제27권6호
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• pp.662-669
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• 2004

The highly water-soluble monomethoxypoly(ethyleneglycol) (mPEG) prod rugs of cyciosporin A(CsA) were synthesized. These prod rugs were prepared by initially preparing intermediate in the form of carbonate at the 3'-positions of CsA with chloromethyl chloroformate, in the pres-ence of a base to provide a 3'-carbonated CsA intermediate. Reaction of the CsA intermediate with mPEG derivative in the presence of a base provides the desired water-soluble prod rugs. As a model, we chose molecular weight 5 kDa mPEG in the reaction with CsA to give water soluble prodrugs. To prove that the prod rug is decomposed in the body to produce CsA, the enzymatic hydrolysis test was conducted using human liver homogenate at $37^{\circ}C$. The prodrug was decomposed in human liver homogenate to produce the active material, CsA, and the hydrolysis half-life ($t_{1/2}$) of the prodrug, KI-306 was 2.2 minutes at $37^{\circ}C$. However, a demon-stration of non-enzymatic conversion in pH 7.4 phosphate buffer was provided by the fact that the half-life ($t_{1/2}$) is 21 hours at 37$^{\circ}C$. The hydrolysis test in rat whole blood was also conducted. The hydrolysis was seen with half-life ($t_{1/2}$) of about 9.9, 65.0, 14.2, 3.4, 2.1 9.5, and 1.6 minutes for KI-306, 309, 312, 313, 315, 316, and 317, respectively. This is the ideal for CsA prodrug. The pharmacokinetic study of the prodrug, KI-306, in comparison to the commer-cial product (Sandimmune Neoral Solution) was also carried out after single oral dose. Each rat received 7 mg／kg of CsA equivalent dose. Especially, the prodrug KI-306 exhibits higher AUC and $C_{max}$ than the conventional Neoral. The AUC and $C_{max}$ were increased nearly 1.5 fold. The kinetic value was also seen with $T_{max}$ of about 1.43 and 2.44 hours for KI-306 and Neoral, respectively.

• Journal of Electrochemical Science and Technology
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• 제8권1호
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• pp.43-52
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• 2017

In this paper, for the first time, the electroanalytical study of Triethylenediamine, TEDA was done on a typically graphene modified carbon paste electrode (Gr/CPE) in pH=10.5 of phosphate buffer solutions (PBS). The surface morphology of the bare and modified electrodes was characterized by scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The electro-oxidation of TEDA was investigated at the surface of modified electrode. The results revealed that the oxidation peak current of TEDA at the surface of Gr/CPE is 2.70 times than that shown at bare-CPE. A linear calibration plot was observed in the range of 1.0 to 202.0 ppm. In this way, the detection limit was found to be 0.18 ppm. The method was then successfully applied to determination of TEDA in aqueous samples obtained from two kinds of activated carbon from the masks of war. On the other hand, density functional theory (DFT) method at B3LYP/6-311++G** level of theory and a conductor-like Polarizable Continuum Model (CPCM) was used to calculate the $pK_a$ values of TEDA. The energies of lowest unoccupied molecular orbital ($E_{LUMO}$) and highest occupied molecular orbital ($E_{HOMO}$), gap energy (${\Delta}E$) and some thermodynamic parameters such as Gibbs free energy of TEDA and its conjugate acid ($HT^+$) were calculated. The results of calculated $pK_a$ were found to be in good agreement with the experimental values.

• 한국통신학회논문지
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• 제25권8B호
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• pp.1424-1435
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• 2000

객체기반 동영상 부호화에서 고정 비트율을 얻도록 하는 율제어기는 채널의 비트율원하는 화질 객체간 분배 텍스쳐 및 형상간 관계 프레임스킵 결정 등에 적응성을 부여하기 위한 중요한 요소이다 따라서 이 논문에서는 울제어에 이용되는내용기반 제어기를 제안한다 이것은 이차 율제어 모델을 이용하는 MPEG-4 기법을 확장한 것이다. 객체간 중요성을 분석하여 VOP로 나뉘어지고 객체기반 모델링에 의해 비트가 할당된다, 중요성을 팍악하기 위해 시험 영상ㅇ을 비전문가에게 관측토록 하여 관심영역 VOP를 분석하였다 이에 의해 초기 목표치가 구해지고 오버플로우나 언더플로우를 방지하기 위해 전체 목표치를 설정한다. 객체간 목표치 분배는 움직임벡터 크기 객체의 크기 이전 프레임의 왜곡 등을 반영하는 통계적 특성에 따라 이루어짐과 동시에 객체의 중요성에 따라 분배하는 기법을 제안한다 제안한 기법을 MPEG-4 VM8 부호기에 사용된것과 비교한다.

• KSBB Journal
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• 제11권6호
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• pp.676-680
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• 1996

키토산을 10%-아세트산에 팽윤시킨 용액과 SD 를 인산염 완충용액에 녹인 용액을 흔합하여 키토산 매트릭스를 제조하였다. 키토산 매트릭스로부터 pH 7 7.4와 pH 1.2에서 약물방출 거통을 규명하고 지속적이고 조절된 약물방출형 제제로서의 사용 가능성 을 고찰하였다. 키토산 매트릭스내의 약물 함유량이 증가함에 따 라 약물 방출 시간이 늦어졌으며, pH 1.2에서보다 p pH 7.4에서 약물 방출 시간이 더 지연되었다. 그 이 유는 약물전달체가 엽기성 용액에서보다 산성 용액 에서 팽윤를 더 잘하기 때문이라고 생각된다. 약물 방출 속도에 있어서는 pH 7.4에서보다 pH 1.2에서 더 빨랐으며, 겉보기 방출속도상수(K)값도 역시 증가하였다. 결과적으로 본 실험에셔 약물전달체로 사 용된 키토산은 방출조절형 제제로서 그 가능성을 타진할 수 있었다.

• 한국환경생태학회지
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• 제15권4호
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• pp.408-419
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• 2002

본 연구는 백두대간의 통합적 관리를 위한 관리공간구획모형의 필요성으로부터 계획되었으며. 개념적인 백두대간의 관리범위모형을 수립할 목적으로 수행되었다 이를 위해 국.내외 공간구획 관련 이론 및 연구의 문헌고찰을 통하여 토지이용 및 관리의 범위 설정의 개념을 파악하고 이를 토대로 개념적인 백두대간관리모형 수립에 접근하고자 하였다. 제시된 관리범위모형은 탄력적 관리를 위해 백두대간의 관리범위를 적극적 보전, 완충, 다목적 이용의 세 구역으로 구분하고 있으며 이에 적합한 관리목표를 설정하여 관리하도록 되어 있다. 각 구역의 구획은 생태적 환경, 물리적 환경, 그리고 사회.문화적 환경을 대표하는 지표인자들의 평가에 의해 구분될 수 있다. 제시된 모형의 효율적인 적용은 백두대간 공간구획을 위한 적절한 지표인자의 선정 및 그에 대한 적합한 평가에 의해 좌우된다고 할 수 있다 그러므로 후속 연구를 통해 그 효율성 또는 효과성이 객관적으로 평가되어야하며 사례연구를 통해 적절한 지표인자 선정 및 평가기준의 마련에 대한 접근이 이루어져야 할 것이다