• 한국축산식품학회지
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• 제40권5호
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• pp.689-698
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• 2020

The aim of study was to scrutinize the physicochemical and protein profile of milk obtained from local Pakistani breeds of milch animals such as Nilli-Ravi buffalo, Sahiwal cow, Kajli sheep, Beetal goat and Brela camel. Physicochemical analysis unveiled maximum number of total solids and protein found in sheep and minimum in camel. Buffalo milk contains the highest level of fat (7.45%) while camel milk contains minimum (1.94%). Ash was found maximum in buffalo (0.81%) and sheep (0.80%) while minimum in cow's milk (0.71%). Casein and whey proteins were separated by subjecting milk to isoelectric pH and then analyzed through sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The results showed heterogeneity among these species. Different fractions including α_S1, α_S2, κ-casein, β-casein and β-lactoglobulen (β-Lg) were identified and quantitatively compared in all milk samples. Additionally, this electrophoretic method after examining the number and strength of different protein bands (α_S1, α_S2, β-CN, α-LAC, BSA, and β-Lg, etc.), was helpful to understand the properties of milk for different processing purposes and could be successfully applied in dairy industry. Results revealed that camel milk was best suitable for producing allergen free milk protein products. Furthermore, based on the variability of milk proteins, it is suggested to clarify the phylogenetic relationships between different cattle breeds and to gather the necessary data to preserve the genetic fund and biodiversity of the local breeds. Thus, the study of milk protein from different breed and species has a wide range of scope in producing diverse protein based dairy products like cheese.

• Journal of Animal Science and Technology
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• 제58권1호
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• pp.2.1-2.10
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• 2016

Background: Heat stress adversely affects the physiological and metabolic status, and the productive performance of buffalo. Methods: The present study was conducted to explicate the effect of misting and wallowing cooling strategies during heat stress in lactating Murrah buffalo. The study was conducted for three months (May-July) of which first two months were hot dry and last month was hot humid. Eighteen lactating buffaloes, offered the same basal diet, were blocked by days in milk, milk yield and parity, and then randomly allocated to three treatments: negative control (no cooling), cooling by misting, and cooling by wallowing. Results: The results showed higher (P < 0.05) milk yield in buffaloes of misting and wallowing group compared to control during the experimental period however wallowing was found more (P < 0.05) effective during July (hot humid period). Both the treatments resulted into significant (P < 0.05) reduction in rectal temperature (RT) and respiratory rate (RR) compared to control animals during study period whereas wallowing was found to be effective on pulse rate (PR) only during July. Both treatments were resulted in mitigating the heat stress mediated decrease in packed cell volume (PCV), lymphocytopnoea and neutrophilia whereas decrease in total erythrocyte count (TEC) and monocytes was only mitigated by wallowing. Heat load induced alteration in serum creatinine and sodium concentration was significantly (P < 0.05) ameliorated by misting and wallowing whereas aspartate aminotransferase, alkaline phosphatase and superoxide dismutase activity, and reactive oxygen species concentration could be normalized neither by misting nor by wallowing. The significant (P < 0.05) increment in serum cortisol and prolactin levels observed in June and July period in control animals was significantly (P < 0.05) prevented by misting and wallowing. Conclusions: It can be concluded that misting and wallowing were equally effective in May and June (hot dry period) whereas wallowing was more effective during hot humid period in preventing a decline in milk production and maintaining physiological, metabolic, endocrine and redox homeostasis.

• Asian-Australasian Journal of Animal Sciences
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• 제10권5호
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• pp.519-522
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• 1997

Buffalo heifers weighing from 400 - 500 kg and having a history of reproductive problems like anestrus, irregular estrus or failure to conceive after repeated inseminations were administered subcutaneously with estradiol-$17{\beta}$ and progesterone in two dosage rate 0.1 mg and 0.25 mg, respectively, per kg body weight per day for 7 days in experiment-I and 0.1 mg and 0.125 mg, respectively, per kg body weight per day for 7 days in experiment II. In experiment-I, 9 out of 10 buffaloes responded positively to the hormonal treatment. Milk secretion started between 14-20 days after the start of the treatment. The total milk yield in the successfully induced animals varied from 471.98-625.40 kg. The average daily milk yield varied from 2.08-2.76 kg and peak yield from 3.6-5.3 kg. The time taken to reach peak yield varied from 12-14 weeks. In experiment - II, the established lactation response was absent, although milk secretion process was initiated, the yield could not reach more than 50 - 100 gm at each milking. In experiment - I, the first estrus occurred between days 87 - 231 following the hormonal treatment. Four animals in which lactation was established successfully got pregnant after one or two services. In experiment - II the first estrus occurred between 85 - 173 days following the treatment and only one animal got pregnant.

• Asian-Australasian Journal of Animal Sciences
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• 제5권2호
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• pp.375-382
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• 1992

The effect of certain factors such as sex of calf, status of buffalo, season of calving, parity and sire on lactation length and total lactation yield was studied in 391 Nili-Ravi buffaloes. The average lactation length was $301.73{\pm}1.87$ (mean $\pm$ SE) days with a range of 181 to 505 days whereas the average lactation yield was $2031.08{\pm}19.16kg$ and ranged from 1023 to 6535 kg for 984 lactations. The differences in the means of lactation length and lactation yield due to the sex of calf were significant (p<0.05). The status of buffaloes had a significant (p<0.05) effect on lactation length but its effect on lactation yield was non-significant. The season of calving had no effect on lactation length but it influenced the lactation yield significantly. The milk yield was highest ($2150.81{\pm}43.52kg$) in buffaloes which calved in spring and lowest ($1959.92{\pm}30.83kg$) in autumn. The effect of parity on both traits under study was significant (p<0.01). The maximum and minimum lactation lengths of $309.82{\pm}3.96$ and $284.16{\pm}7.17$ days were observed in the first and sixth lactations, respectively. The milk yield was maximum ($2150.38{\pm}58.79kg$) in the seventh lactation and minimum (1818.31 60.04 kg) in the sixth lactation. The influence of sire was significant on lactation length (p<0.05) and milk yield (p<0.01).

• Asian-Australasian Journal of Animal Sciences
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• 제20권1호
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• pp.124-131
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• 2007

There is worldwide interest in buffalo as an animal for meeting the growing demands of meat, milk and work in the developing countries. One of the major constraints to full exploitation of the productive potential of buffalo has been its inherently low reproductive efficiency as reflected by late maturity, poor expression of oestrus, silent oestrus, irregular oestrous cyclicity, seasonality in breeding, anoestrus, low conception rate, long postpartum interval, repeat breeding etc. Ovarian cyclicity is regulated by endocrine and neuroendocrine mechanisms namely hypothalamic hormones, gonadotropins and ovarian steroids. Detailed endocrine investigations are suggested with special reference to the hypothalamo-hypophysial-ovarian axis to gain a better understanding of reproduction in buffalo and to modify it to derive the maximum benefit from this animal.

• Asian-Australasian Journal of Animal Sciences
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• 제13권9호
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• pp.1329-1336
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• 2000

Two experiments were carried out to investigate the effect of Murrah buffalo cows pulling sledges under field conditions on milk production and milk composition. In Exp. 1, 24 buffaloes in the fourth month of lactation were used. They were allotted to four treatments according to a $2{\times}2$ factorial arrangement: work or no work, and with or without urea-molasses cake supplementation (700 g/animal/day). Feeds consisted of 20 kg fresh elephant grass (18% DM), 2 kg rice bran per day and rice straw ad lib. The animals worked in pairs three hours per day (work done: $3464{\pm}786kJ/d$) five days a week for three months. Three teams worked in the morning and the others worked in the afternoon in the same day. The following day the working times were switched. In Exp. 2, 16 lactating Murrah buffalo cows in the sixth month of lactation were allotted to two groups (work and no work). They were fed with fresh ruzi grass (Brachiaria ruziziensis) ad lib. supplemented with 2 kg rice bran and 700 g urea-molasses cake. The working regime was similar to that of the first experiment (work done: $3753{\pm}879kJ/d$) and they worked for two months. In the first experiment, there was a small but significant drop (p<0.05) in milk yield from 3.5 to 3.0 kg/day due to work, but there was no supplementation effect. The working buffaloes lost 5.2 kg whereas the non-working animals gained 9.7 kg during the three months (p<0.05). Supplementation increased live weight by 9.9 kg as compared to -5.4 kg for those not supplemented (p<0.05). Milk composition was not affected by the treatments. In the second experiment, daily milk production was similar for both treatments and approximately 3 kg. No significant differences were found in milk composition or in live weight changes for working and non-working groups, respectively. It was concluded that work may cause a reduction in milk yield and a loss of live weight on a poor rice straw diet but that an appropriate supplementation can alleviate this situation.

• Asian-Australasian Journal of Animal Sciences
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• 제14권4호
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• pp.474-478
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• 2001

Eighteen Murrah male buffalo calves were allotted into three groups of six each. The calves in group I (control) were fed with whole milk, skim milk, calf starter and green maize fodder. The calves in group II (high butyric acid) and group III (low butyric acid) were fed with the same diet as control along with 24 ml and 12 ml of butyric acid/calf/day for 120 days, respectively for 120 days. Dry matter intake was higher in group II and III as compared with group I. Digestibility of dry matter, organic matter, crude protein, crude fibre, ether extract, neutral detergent fibre, acid detergent fibre, cellulose and hemicellulose was the highest in group II followed by group III and the control group. Body weight gain and conversion efficiency of dry matter, digestible crude protein and total digestible nutrients were better in group II. Cost of feed for per unit of live weight gain was the lowest in group II. It was concluded that dietary addition of butyric acid (24 ml/day) was economical and had positive effect on the performance of Murrah buffalo calves.

• Asian-Australasian Journal of Animal Sciences
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• 제13권5호
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• pp.580-586
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• 2000

Data collected between 1981 and 1991 at the Philippine Carabao Center at Central Luzon State University (PCC-CLSU) were used for the comparison of growth, milk yield and draughtability of Murrah-Philippine crossbred and Philippine native buffaloes. Body weights and body measurements were available at 3-month intervals from birth to 36 months of age for a total of 34 $Murrah{\times}Philippine$ native buffalo F1 crossbreds (CBB; 21 cows, 13 bulls) and 32 Philippine native buffaloes (PNB; 16 cows, 16 bulls). Lactation records were available for 14 CBB and 19 PNB cows. Data for draughtability under wet and dry ploughing conditions were available for 4 CBB and 4 PNB steers. The results indicate that crossbreds grow faster (0-9 months of age: cows $442{\pm}19$ vs. $301{\pm}21g/day$, bulls $305{\pm}23$ vs. $296{\pm}21g/day$; 9-36 months of age: cows $227{\pm}10$ vs. $147{\pm}12g/day$, bulls $282{\pm}13$ vs. $138{\pm}12g/day$), mature earlier and produce more milk (1st lactation: $1139{\pm}153$ vs. $450{\pm}112kg$; 2nd lactation: $1115{\pm}132$ vs. $488{\pm}136kg$) than native buffaloes, but have a poorer draughtability (wet ploughing; force as % of body weight $8.8{\pm}0.2$ vs. $12.2{\pm}0.6$; dry ploughing: cut depth $10.98{\pm}0.25$ vs. $11.92{\pm}0.13cm$, velocity $0.50{\pm}0.03$ vs. $0.60{\pm}0.02m/sec$, force as % of body weight $9.0{\pm}0.6$ vs. $11.3{\pm}0.7$). The correlation coefficients between body weight and body measurements at birth and at 3-month intervals indicate that heart girth has a relatively high correlation with body weight, especially in crossbreds. It is concluded that in Philippine smallholder farming systems in which meat and milk production are secondary to draught power, the native buffalo is preferable from the point of view of input needed to maintain the number of animals kept for a required draught force.

• Asian-Australasian Journal of Animal Sciences
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• 제11권5호
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• pp.470-474
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• 1998

Data on the first lactation performance traits of $F_1$, $F_2$ and $F_3$ crosses covering the period from 1972 to 1995 of a total of 803 dairy cows of three genetic grades maintained at Livestock Production Research Farm, Indian Veterinary Research Institute, Izatnagar were analysed. Three genetic grades were 1/2 Friesian + 1/2 Hariana (FH), 1/2 Friesian + 1/4 Brown Swiss and 1/4 Hariana (FBH) and 1/2 Friesian+ 1/4 Jersey + 1/4 Hariana (FJH). Age at first calving increased by 7% and 8% in $F_2$ and $F_3$, respectively, over the $F_1$ in FH. The reduction in age at first calving at $F_2$ and $F_3$ levels by 2-7% over the $F_1$ was observed in FBH and FJH. The lactation milk yield of $F_1$, $F_2$ and $F_3$ crosses was $1,943{\pm}100.3$, $2202{\pm}120.5$ and $1,925{\pm}123.2kg$ in FH; $2,014{\pm}76.7$, $2,264{\pm}91.5$ and $2,096{\pm}123.9kg$ in FBH and $2,005{\pm}87.0$, $2,414{\pm}94.4$ and $2,093{\pm}121.1kg$ in FJH, respectively. The lactation milk yield improved by 12-20% in $F_2$ crosses in various genetic grades. The performance of $F_1$ was, however, maintained in FH $F_3$ crosses, it improved by 4% in FBH and FJH $F_3$ crosses. The lactation lengths and calving intervals were nearly the same for $F_1$, $F_2$ and $F_3$ crosses in FH while lactation lengths and calving intervals were reduced by 3-11% in $F_2$ and $F_3$ crosses in FBH and FJH genetic grades. The milk yield/day of lactation length and milk yield/day of calving interval increased by 16-35% in $F_2$ and 2-14% in $F_3$ over the $F_1$ in various genetic grades. It is recommended that a sufficiently large effective population size of these three genetic grades be maintained by inter se matings and rigorous selection of sires so for developing a genetic base population for new breed development.

• 한국축산식품학회지
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• 제31권1호
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• pp.1-8
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• 2011

A unique biophysical membrane which surrounds the milk fat globules is called the milk fat globule membrane (MFGM). Various researches were studied about origin, composition, structure and bioactive components of MFGM. Bioactive protein components of MFGM play an important beneficiary function such as defense mechanism in new born. Among the bioactive lipid components from MFGM phospholipids showed health enhancing functions. The phospholipids also help in the production of certain dairy product from deterioration. MFGM phospholipids also showed antioxidant activity in some dairy products such as butter and ghee produced from milk of buffalo. Based on the beneficial effects, researchers developed MFGM as functional ingredients in various food products. This current review focuses on health enhancing function of MFGM and its components in various dairy products.