• Title/Summary/Keyword: Budding

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Studies on Witches' Broom of Ligustrum ovalifolium Hasskarl Caused by Mycoplasma-like Organism (MLO) (Mycoplasma성(性) 왕쥐똥나무 빗자루병(病)에 관(關)한 연구(硏究))

  • Chai, Jyung-Ki;Kim, Young-Ho
    • Journal of Korean Society of Forest Science
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    • v.78 no.2
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    • pp.103-118
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    • 1989
  • The occurrence of witches' broom in Ligustrum ovalifolium was first noticed in Korea by author in 1984. The present study was carried out with particular emphasis on the symptomatology, etiology, transmission of the disease and antibiotic treatments. The infected tissue was observed by the fluorescence and electron microscopy and its biochemical characteristics were compared with healthy one by electrophoresis. The results are summarized as follows : 1. symptoms of the infected trees were characterized by the dwarfing of the organs, yellowing and brooming of the foliage. 2. The observation by the trans electron microscopy on the witches' broom of L. ovalifolium revealed the occurrence of numerous mycoplasma-like organisms(MLOs) in the phloem tissue cells of the midribs of infected leaves. 3. The MLOs were surrounded by a single unit membrane, and they appeared to be multiplied by binary fission. 4. The presence of crystals unidentified in the phloem parenchyma cells was noticed by electron rnicroscopy, 5. The disease was able to be transmitted by budding, crown, and greenwood graftings to L. ovalifolium, L. obtusifolium, L, japonicum and also transmitted, even when the stocks and scions were not completely grafted. 6. Insect transmission on L. ovalifolium and L, obtzrsifolium was carried by Hishimonus sellatus. 7. The infected roots dipped in the 1,000 ppm of teracyclin solution was only temporarily effective in controlling the disease. 8. Infected plant with MLOs showed specific fluorescent reactions in phloems with DAPI stain. 9. The protein and peroxidase separated by electrophoresis showed strikingly distinctive difference between the healthy and diseased leaves.

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Reduction of Stem Cavity and Improvement of Flower Quality in Chrysanthemum 'Baekma' by Hydroponic Culture (양액재배에 의한 국화 '백마'의 줄기공동 경감과 절화품질 향상)

  • Hwang, In Taek;Cho, Kyung Chul;Kim, Hee Gon;Ki, Gwang Yeon;Yoon, Bong Ki;Kim, Jung Guen;Lim, Jin Hee;Choi, Sung Ryul;Shin, Hak Ki
    • FLOWER RESEARCH JOURNAL
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    • v.17 no.4
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    • pp.251-255
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    • 2009
  • This study was conducted to investigate effect of ionic strength and feeding times of a nutrient solution on reduction of stem cavity size and improvement of flower quality in chrysanthemum 'Baekma'. A nutrient solution was applied with different strengths at three stages, namely, transplanting, budding, and flowering. The solution EC was adjusted as 1.61.82.0, 1.81.81.8, 1.82.01.8, and $2.02.02.0dS{\cdot}m^{-1}$ in four treatments. Feeding frequency per a day were 4 times for 12 min., 8 times for 6 min., 12 times for 4 min., and 18 times for 2.7 min. each. Cut flower length as affected by different strengths of a nutrient solution was the greatest in the plot of EC $2.02.02.0dS{\cdot}m^{-1}$. However number of leaves, stem diameter, and leaf size were greater in EC $1.82.01.8dS{\cdot}m^{-1}$ than in other treatments. Also, petal number of petals was the greatest and stem cavity size was the smallest in the plot of EC $1.81.81.8dS{\cdot}m^{-1}$. Plant height, number of leaves, stem diameter, leaf size were greater in the plot with 12 times feed ing per a day. number of petal was most in the plot with 8 times feeding per day, while stem cavity size was the smallest in the plot with 12 times feed ing per a day. Therefore, the better plant growth, the smaller stem cavity size.

Effect of Soil Mulching Materials and Methods on Weed Occurring for the Growth and Flowering in Gypsophila paniculata Cultivation (토양피복에 따른 잡초발생과 안개초의 생육 및 개화에 미치는 영향)

  • Cheong, Dong-Chun;Oh, Jeong-Moon;Lim, Hoi-Chun;Song, Young-Ju;Kim, Jeong-Man
    • FLOWER RESEARCH JOURNAL
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    • v.19 no.1
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    • pp.15-21
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    • 2011
  • This study investigated the effect of soil mulching materials and methods on weed occurring for the growth and flowering characteristics in Gypsophila plants cultivation. For mulching materials were used black polyethylene (PE) film, black polypropylene (PP), and green polypropylene, and these were mulched in both ridge and furrow (BRF) or only furrow, respectively. The weed was occurred total $146.2g/m^2/yr$ as dry weight in non-mulching, but $4.1{\sim}4.2g/m^2/yr$ in BRF mulching by black or green PP. Also weed control were yearly required 27.9 persons/10a in non-mulching, whereas 2.4 persons/10a in BRF mulching by black or green PP. Flower budding and blooming were roughly delayed in non-mulching and furrow mulching. Flowering characteristics such as flower stalk length, primary branches number, and stem firmness were remarkably improved in BRF mulching of black or green PP. Cut flower yield was increased on black BRF mulching or green PP in summer cultivation, while green PP BRF mulching showed better yield in in autumn cultivation. Mortality rate of Gypsophila plants after summer season was lowest as 12.4% in furrow mulching with black PP, followed by about 19.0% in green PP mulching, but highest as 39.0% in BRF mulching with black PE film. Rosette formation rate was lower in furrow than BRF mulching, and was lowest as 13.1% in BRF mulching with green PP, followed by 15.2~15.8% in BRF mulching with black PE film or black PP. So it was thought that BRF mulching with green PP was highly effective in weed control and improvement of yield and cut flower quality in Gypsophila plants cultivation.

Effect of Temperature, Glasshouse Forcing Date and GA3 on the Growth and Flowering of Hydrangea macrophylla Ser. (온도, 입실시기 및 GA3 처리가 수국의 생육 및 개화에 미치는 영향)

  • Lee, Hee Doo;Kim, Si Dong;Kim, Ju Hyoung;Lee, Jong Won;Kim, Tae Jung;Lee, Cheol Hee
    • FLOWER RESEARCH JOURNAL
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    • v.16 no.4
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    • pp.260-265
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    • 2008
  • Studies were carried out to elucidate the effect of temperature, glasshouse forcing date and $GA_3$ on the growth and flowering of pot Hydrangea macrophylla Ser. The plant height was elongated in the $5^{\circ}C$ treatments as 38 cm compared with $20^{\circ}C$ treatment as 6.7 cm, and stem length showed the similar results. The leaf length and width was broadened in the lower temperature, and stem diameter showed the same tendency. The first flowering date in the $15^{\circ}C$ and $20^{\circ}C$ treatment shortened markedly than $5^{\circ}C$ treatment. Days to flowering date of $5^{\circ}C$ was 161 day, while it shortened as 88 day in the $15^{\circ}C$ treatment. The flowering rate was 75.8~90.7% in the temperature. The plant height was elongated in the late glasshouse forcing date, and the leaf length and width showed the similar tendency. The plant height increased in the higher concentration of $GA_3$ compared to the control, and leaf length and width showed the similar results. The first flowering day was advanced by 7 days in the $GA_3$ $50mg{\cdot}L^{-1}$ treatment which had been transferred to greenhouse on Dec. 30 compared with the control of which first flowering day was March 17, and the days to first flowering was conspicuously shortened in the late glasshouse forcing treatment. The width of flower cluster was increased in the $GA_3$ at Dec. 30 glasshouse forcing treatment. The flowering rate was markedly decreased as 62.3% in the control of Nov. 15 treatment, but was increased as 97.9% of $GA_3$ $50mg{\cdot}L^{-1}$ of Jan. 15 treatment.

Studies on the Physiological Chemistry of Seed Development in Ginseng Seed (인삼식물의 종자발육 과정에 있어서의 생리화학적 연구)

  • Hee-Chun Yang
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.17
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    • pp.115-133
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    • 1974
  • This study was done on the metabolism of chemical components during the seed development of ginseng. The changes of the chemical components were inspected in the following periods: from the early stage of flower organ formation to flowering time, from the early stage of fruiting to maturity, during the moisture stratification before sowing. From flower bud forming stage to meiosis stage, the changes in the fresh weight, dry weight, contents of carbohydrates, and contents of nitrogen compounds were slight while the content of TCA soluble phosphorus and especially the content of organic phosphorus increased markedly. From meiosis stage to microspore stage the fresh and dry weights increase greatly. Also, the total nitrogen content increases in this period. Insolub]e nitrogen was 62-70% of the total nitrogen content; the increase of insoluble nitrogen seems to have resulted form the synthesis of protein. The content of soluble sugar (reducing and non-reducing sugar) increases greatly but there was no observable increase in starch content. In this same period, TCA soluble phosphorus reached the maximum level of 85.4% of the total phosphorus. TCA insoluble phosphorus remained at the minimum content level of 14.6%. After the pollen maturation stage and during the flowering period the dry weight increased markedly and insolub]e nitrogen also increased to the level of 67% of the total nitrogen content. Also in this stage, the organic phosphorus content decreased and was found in lesser amounts than inorganic phosphorus. A rapid increase in the starch content was also observed at this stage. In the first three weeks after fruiting the ginseng fruit grows rapidly. Ninety percent of the fresh weight of ripened ginseng seed is obtained in this period. Also, total nitrogen content increased by seven times. As the fruits ripened, insoluble nitrogen increased from 65% of the total nitrogen to 80% while soluble nitrogen decreased from 35% to 20%. By the beginning of the red-ripening period, the total phosphoric acid content increased by eight times and was at its peak. In this same period, TCA soluble phosphorus was 90% of total phosphorus content and organic phosphorus had increased by 29 times. Lipid-phosphorus, nucleic acid-phosphorus and protein-phosphorus also increased during this stage. The rate of increase in carbohydrates was similar to the rate of increase in fresh weight and it was observed at its highest point three weeks after fruiting. Soluble sugar content was also highest at this time; it begins to decrease after the first three weeks. At the red-ripening stage, soluble sugar content increased again slightly, but never reached its previous level. The level of crude starch increased gradually reaching its height, 2.36% of total dry weight, a week before red-ripening, but compared with the content level of other soluble sugars crude starch content was always low. When the seeds ripened completely, more than 80% of the soluble sugar was non-reducing sugar, indicating that sucrose is the main reserve material of carbohydrates in ginseng seeds. Since endosperm of the ripened ginseng seeds contain more than 60% lipids, lipids can be said to be the most abundant reserve material in ginseng seeds; they are more abundant than carbohydrates, protein, or any other component. During the moisture stratification, ginseng seeds absorb quantities of water. Lipids, protein and starch stored in the seeds become soluble by hydrolysis and the contents of sugar, inorganic phosphorus, phospho-lipid, nucleic acid-phosphorus, protein phosphorus, and soluble nitrogen increase. By sowing time, the middle of November, embryo of the seeds grows to 4.2-4.7mm and the water content of the seeds amounts to 50-60% of the total seed weight. Also, by this time, much budding material has been accumulated. On the other hand, dry stored ginseng seeds undergo some changes. The water content of the seeds decreases to 5% and there is an observable change in the carbohydraes but the content of lipid and nitrogen compounds did not change as much as carbohydrates.

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