• 농업과학연구
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• 제39권1호
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• pp.81-86
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• 2012

In this study, the antioxidative effect and protective potential against oxidative damage of extract and fractions from broccoli were investigated under in vitro and cellular system. The methanol (MeOH) extracts of broccoli flowers and sprouts were partitioned as dichloromethane, n-butanol (BuOH) and aqueous fractions. The comparison of antioxidative effect of broccoli flowers and sprouts showed that broccoli sprouts exerted the more effective protective activity from 2,2'-azobis (2-aminopropane) dihydrochloride (AAPH)-induced oxidative stress in LLC-$PK_1$ porcine renal epithelial cell. In addition, the extract and fractions from broccoli sprouts showed strong scavenging effect of 1,1-diphenyl-2-picrylhydrazyl radical and the BuOH fraction exerted the strongest activity. Therefore, the BuOH fraction was evaluated as the most active fraction with strong radical scavenging activity among the fractions of broccoli flowers and sprouts. The present study suggests the antioxidative potential against free radical-induced oxidative damage of flowers and sprouts of broccoli. In addition, the BuOH fraction of broccoli is considered as the active fraction with antioxidative effect.

• 한국식품영양과학회지
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• 제34권6호
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• pp.759-764
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• 2005

브로콜리의 잎, 꽃, 꽃-줄기,줄기 그리고 껍질을 에탄올, 아세톤 및 증류수로 추출하여 그 추출물의 라디칼소거 활성을 조사하였다 각각의 시료는DPPH 라디칼에 대한 라디칼 소거 활성 (FRSA)을 나타내었다. 다섯 가지의 시료 중, 줄기 추출물, 꽃 추출물 및 잎 추출물에서 dot-blot 분석을 통해 라디칼소거 활성을 확인하였다. 브로콜리의 에탄을 추출물 에서는 $pH\;2\~6$의 산성영역 및 $60\~80^{\circ}C$의 온도 범위에서 강력한 라디칼소거 활성을 보였다. 녹차 추출물과 쑥 추출물에서는 Staphylococcus aureus에 대한 항균성을 보였고, 다섯 가지의 추출물중에서 단지 꽃 추출물 및 꽃-줄기 추출물이 고온 하에서 Bacillus arnyloliquefaciens에 대해 강력한 항균성을 나타내었다.

• 동아시아식생활학회지
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• 제19권4호
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• pp.564-569
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• 2009

Antioxidative activities, total phenolic compounds and the sulforaphane contents of cooked broccoli extracts were studied. Total phenolic compounds were determined to be 0.96 mg/g(flower) and 0.76 mg/g(stem) in fresh broccoli extracts. The total phenolic compounds of cooked broccoli extracts showed 0.92 (1 min), 0.79 (3 min), 0.67 (10 min) mg/g when a boiling process was used and 1.27 (1 min) mg/g when a steaming process was used. In the DPPH assay, the steam process showed the highest free radical scavenging capacities. Sulforaphane has been of increasing interest in the nutraceutical and phamaceutical industries due to its anti-cancer effect. Sulforaphane was isolated from fresh and boiled, steamed broccoli using dichloromethane as an extract solvent. The sulforaphane contents of fresh broccoli were higher in the flower (14.78 mg/kg) than in the stem (6.16 mg/kg). The sulforaphane content dramatically decreased after the boiling ($100{\pm}2^{\circ}C$) or steaming ($100{\pm}2^{\circ}C$) processes were used.

• 동아시아식생활학회지
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• 제23권2호
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• pp.141-152
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• 2013

This study investigated the effects of image on the preference and intake frequency of 19 vegetables. A total of 359 usable surveys were collected using a convenient sampling method. The subjects included females (51.8%), university students (50.7%), home residents (66.9%) and subject's spending 20,000~40,000 won on meals/week (41.5%) and eat out 2~3 times/week (29.5%). The intake frequency of vegetables was 2~3 times per month. The healthy image of all vegetables was good overall and the average preference was 3.78 (out of 5 on the Likert). Tomatoes had the healthiest image, onions the highest preference, and Korean cabbage the highest intake frequency. For males, the vegetable with the healthiest image was sesame leaf, while the healthiest foods for females were broccoli and tomatoes. Elementary students had a healthier image of cucumber, bean sprouts, radish, sesame leaf, lettuce, radish leaf, and cabbage than university students and adults. Home residents had a healthier image of cabbage and burdock than other types of residents. Subject that st over 20,000 won per week on meals had a higher image of most vegetables. In terms of preference, males liked Korean cabbage, green pumpkin, balloon flower roots, radish leaf, and lotus root, but female liked tomatoes. In addition, elementary students, home residents, and subjects who eat out less often tended to prefer vegetables. In terms of intake, there was a high frequency of intake for all vegetables in adults. Home residents specifically had a higher intake of cucumber, carrot, bean sprouts, spinach, green pumpkin, balloon flower roots, lettuce, radish leaf, broccoli, burdock, lotus root, and tomato. Overall, the healthy image of vegetables had a positive influence on their preference and intake frequency. Therefore, to encourage the intake of vegetables, direct or indirect variables should be examined.

• 원예과학기술지
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• 제30권6호
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• pp.743-750
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• 2012

본 연구는 UPLC를 이용하여 국내 브로콜리 육성계통 95점의 화구 내 desulfo-glucosinolate를 표준물질과 비교하여 정량 분석하고 그 함량변이를 평가하여, 궁극적으로 고기능성 국내 브로콜리 품종 육성의 기초자료로 이용하고자 수행되었다. 브로콜리 화구에서 9종의 표준물질과 비교하여 progoitrin, glucoraphanin, sinigrin, gluconapin, glucobrassicanapin 및 glucobrassicin 등 6종의 glucosinolate가 동정되었다. 전체 브로콜리 육성계통 95점에 대해서 총 glucosinolate 함량은 $4.2-29.0{\mu}mol{\cdot}g^{-1}$ DW의 변이를 보였다. 총 glucosinolate 중 glucoraphanin의 함량은 $1.6-13.9{\mu}mol{\cdot}g^{-1}$ DW로 가장 높은 비율을 차지하였고 progoitrin은 제한적인 13개의 계통에서만 특이적으로 함유되어 있었으며 glucoraphanin의 함량과는 부의 상관관계를 보였다. 동정된 glucosinolate 중 glucobrassicanapin, glucoraphanin, glucobrassicin 및 gluconapin의 4종 물질이 전체 브로콜리 계통의 함량변이에 크게 영향하였고 이들 4종 물질 상호 간에는 높은 정의 상관관계가 있음이 확인되었다. 본 연구결과에서 선발된 브로콜리 유망계통에 대해서는, 향후 재배 작형 및 연차간 물질함량의 안정성 평가와 유전자원 간 추가적인 비교평가 연구를 통하여 고기능성 육종소재로의 개발이 가능할 것으로 판단된다.