• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.43 no.5
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• pp.299-304
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• 2017

Objectives: To test the feasibility of submandibular salivary gland (SMG) replantation techniques and the survival of the replanted glands. Such a study can provide a rationale for later allotransplantation procedures, along with implementation of conventional and advanced immunosuppression therapy. Materials and Methods: Six SMG replantations were performed in New Zealand white rabbits. One week postoperatively, $^{99m}Tc$ scintigraphy was performed and the uptake ratio and salivary excretion fraction were calculated. Two to four weeks later, submandibular glands were excised, fixed, and stained with H&E for histomorphometric evaluation. Results: Intraoperatively, all glands showed patent blood perfusion except gland 5. Positive tracer uptake and saliva excretion were documented by scintigraphy. On excision, all of the glands except glands 4 and 5 looked viable, with a red color and patent pedicles. Gland 4 was infected and filled with creamy pus, while gland 5 looked pale and necrotic. Histologically, glands 1, 2, 3, and 6 had preserved normal glandular tissue with slight variations from the contralateral normal glands, as their parenchyma was composed of mildly atrophic acini. Conclusion: Four out of six replanted SMGs successfully survived. The glands maintained good viability and function. Such success depends on safe harvesting, short anastomosis time, and strict control of infection.

• Korean Journal of Plant Resources
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• v.33 no.1
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• pp.40-49
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• 2020

The objectives of this study were to evaluate total phenolic content (TPC) and individual phenolic compounds in leaves of perilla genetic resources, assess whether they could be used as distinguishing factor among germplasms, and evaluate their relationship with some quantitative and qualitative morphological characters. TPC and individual phenolic compounds were determined using Folin-Ciocalteu method and UPLC-PDA system, respectively. Wide variations in TPC (7.99 to 133.70 mgGAE/g DE), rosmarinic acid (ND to 21.05 mg/g DE), caffeic acid (ND to 1.17 mg/g DE), apigenin-7-O-diglucuronide (ND to 2.21 mg luteolin equivalent (mgLUE)/g DE), scutellarein-7-O-glucuronide (ND to 5.25 mg LUE/g DE), and apigenin-7-O-glucuronide (ND to 2.81 mg LUE/g DE) were observed. Intensities of green pigment at abaxial and adaxial leaf surfaces were positively correlated with phenolic compounds whereas leaf length and width had negative correlation. Purple pigmented accessions were shorter in leaf length and width but exhibited higher amount of phenolic compounds compared to green pigmented accessions in most cases. Leaf shape was not related with content of phenolic compounds, color of leaves, and length/width of leaves. TPC and individual phenolic compounds along with morphological characters could be useful distinguishing factors for perilla genetic resources.

• Journal of Forest and Environmental Science
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• v.31 no.2
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• pp.78-90
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• 2015

Short rotation woody crops belonging to the genera Populus L., Salix L., Pinus L., and Eucalyptus L'Her. have provided broad economic and ecological benefits throughout the world, including afforestation and reforestation along urban to rural gradients. Within the genus Populus, cottonwoods, poplars, aspens, and their hybrids (hereafter referred to as poplars) have been shown to exhibit favorable genotype ${\times}$ environment interactions, especially in the face of changing climates. Similar growth responses have been reported for Pinus, especially with white pine (Pinus strobus L.) in the North Central United States. This has led to current research priorities focused on ecosystem services for both genera. The Millennium Ecosystem Assessment (2005) defines cultural, supporting, provisioning, and regulating ecosystem services. The overarching objective of this paper was to synthesize information about the potential of poplars to provide multiple ecosystem services when grown at sites with varying soil and climate conditions across landscape gradients from urban to rural areas. Specific objectives included: 1) providing background of the United States Forest Service and its Research and Development branch, 2) integrating knowledge of current poplar breeding and development with biomass provisioning and carbon regulating ecosystem services as they relate to changing climates in the North Central United States, and 3) providing a case study illustrating this integration through comparisons of poplar with white pine. Our results were evaluated in the context of climate change mitigation, with specific focus on selection of favorable genotypes for sequestering atmospheric carbon and reducing fossil fuel carbon emissions.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.57 no.2
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• pp.113-126
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• 2012

Northern corn leaf blight caused by Exserohilum turcicum Pass is considered the most important disease infecting corn (Zea mays L.) in the Peoples' Republic of Korea (North Korea). It contributes to the food shortage in North Korea. The objectives of the current research were to study resistance expression and responses of corn crosses made between ten hybrids from North Korea and inbreeding lines ($S_{3-4}$ stage) from the Republic of Korea (South Korea). The experiments were conducted in six trials with a total of 184 crosses including two commercial hybrids in each trial. The trials were conducted at two locations in North Korea (Mirim and Eunsan) and one location in South Korea (Gunwi) under natural infestation of E. turcicum. Host plant responses were rated on a scale of 1 (highly tolerant) to 9 (highly susceptible). A total of 111 crosses (62.4%) showed significant tolerant or susceptible response variations among three locations; 42 crosses (22.8%) at two locations and 69 crosses (39.0%) at one location, respectively. At least 8 crosses of high level of tolerance and 12 crosses of high level of susceptibility showed significantly different biotic responses (P = 0.05). The results of the current study and historical reviews of E. turcicum epidemics in both North and South Korea suggest that breeding of tolerance with quantitatively inherited genes should be carried out for a sustainable corn production in North Korea.

• Journal of Preventive Medicine and Public Health
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• v.38 no.4
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• pp.482-488
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• 2005

Objectives : We conducted an investigation on 14 cases of brucellosis in Gyeongsangbuk-do during 2003-2004 to understand the source of infection and the transmission routes of brucellosis. Methods : The authors visited the each of the health centers and we examined the patients, their written epidemiologic questionnaire and the occurrence of bovine brucellosis. We visited the patients' living and work areas, and we examined their occupations, the date they developed symptoms, the progress of their symptoms, whether or not they were treated, their current status, whether or not they consumed raw milk and raw meat, and if their work was related to cattle breeding and the related details. We reviewed the results of the blood tests and medical records and we examined the cattle's barn. Results : There were 3 patients in 2003 and 11 patients in 2004. All of their brucella antibody titer exceeded 1:160. The patients' symptoms were fever, myalgia, malaise, chills and an influenza-like illness, but the clinical signs were absent on the medical records. Brucella abortus were cultured from 3 of the patients' blood samples. Conclusions : When the authors discovered the transmission routes, they were divided into 4 different sorts. The first route was related to cattle birth such that patients touched the calves or placentas that were infected with the Brucella species. The second route was related to performing artificial insemination on the cattle and the semen that was used for artificial insemination. The third route was due to the ingestion of raw meat and milk. The last route was due to sexual intercourse between the patients.

• Journal of Life Science
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• v.18 no.12
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• pp.1665-1670
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• 2008

Soybean oil is an important source of vegetable oil for human food and nonfood applications and accounts for approximately 22% of the world's total edible oil production. Improvement of the quality and quantity of soybean seed oil constituents is one of the most important objectives in soybean breeding. The objective of this study was to identify quantitative trait loci (QTLs) that control oleic, linoleic, and linolenic acid contents in soybean. The 117 $F_{2:10}$ recombinant inbred lines (RIL) developed from a cross of 'Keunolkong' and 'Shinpaldalkong' were used. Narrow-sense heritability estimates based on a plot mean on seed weight, protein and oil content were 0.85, 0.82 and 0.81, respectively. Eight independent QTLs for oleic acid content were identified from linkage group (LG) A2, C1, D2, F, G, L, and O. Seven QTLs for linoleic acid content were located on LG D1b, E, H, I and L. Oil content was related with five QTLs located on LG C1, H, J, K, and L. Oleic, linoleic, and linolenic acid have two common QTLs on LG C1 and L. Thus, we identified major loci improving soybean oil quality.

• Food Science and Biotechnology
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• v.18 no.3
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• pp.793-798
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• 2009

The objectives of this present study were to compare the contents and determine optimum extraction conditions for the rosmarinic acid (RA) and caffeic acid (CA) from leaves of Korean Perilla frutescens varieties. RA and CA from leaves of cv. Bora, a breeding line of P. frutescens were isolated and elucidated using various spectroscopic data. On the basis of 2 phenolic acids, optimum extraction conditions were obtained by employing 50% EtOH for 60 min at $25^{\circ}C$. We reported for the first time on the contents of RA and CA from leaves of 32 Korean varieties. Among them, leaves of P. frutescens Brit. var. acuta Kudo I exhibited the highest RA content ($8.53{\pm}0.57$ mg/g) and CA content ($2.33{\pm}0.11$ mg/g) showed the highest in the P. frutescens Brit. var. viridis Makino. Interestingly, average RA content ($2.66{\pm}0.17$ mg/g) showed a markedly higher than that of CA ($1.98{\pm}0.16$ mg/g) in Korean varieties. These results suggest that concentrations of the RA and CA in P. frutescens leaves could be a key factor in the selection process of a high quality species.

• Korean Journal of Plant Resources
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• v.34 no.6
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• pp.575-599
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• 2021

The edible roots of radish (Raphanus sativus L.) are consumed worldwide. For characterization and evaluation of the agronomic traits and health-promoting chemicals in radish germplasms, new germplasm breeding materials need to be identified. The objectives of this study were to evaluate the phenotypic traits and glucosinolate contents of radish roots from 110 germplasms, by analyzing correlations between 10 quantitative phenotypic traits and the individual and total contents of five glucosinolates. Phenotypic characterization was performed based on descriptors from the UPOV and IBPGR, and glucosinolate contents were analyzed using liquid chromatography-tandem mass spectrometry in multiple reaction monitoring mode (MRM). Regarding the phenotypic traits, a significant correlation between leaf length and root weight was observed. Glucoraphasatin was the main glucosinolate, accounting for an average of 71% of the total glucosinolates in the germplasms; moreover, its content was significantly correlated with that of glucoerucin, its precursor. Principal component analysis indicated that the 110 germplasms could be divided into five groups based on their glucosinolate contents. High levels of free-radical scavenging activity (DPPH) were observed in red radishes. These results shed light on the beneficial traits that could be targeted by breeders, and could also promote diet diversification by demonstrating the health benefits of various germplasms.

• Journal of Veterinary Science
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• v.21 no.5
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• pp.60.1-60.11
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• 2020

Background: Tumor-associated neoangiogenesis is a crucial target for antitumor therapies. Thalidomide (TAL) is a promising anti-neoangiogenetic drug that has recently been used in the treatment of several malignancies in dogs. Objectives: The aim of the study was to assess the pharmacokinetics of TAL after single oral administration in dogs. Additionally, the influence of feeding on the pharmacokinetic profile of TAL in dogs has been preliminarily investigated. Methods: Six healthy adult female Labradors were enrolled according to a randomized single-dose, 2-treatment, 2-phase, paired 2 × 2 cross-over study design. The dogs were administered a single 400 mg capsule of TAL in fasted and fed conditions. Blood was collected from 15 min to 48 h after dosing, and TAL quantified in plasma by a validated high-performance liquid chromatography method. The pharmacokinetics of TAL were analyzed using a non-compartmental approach. Results: TAL concentration was quantifiable up to 10 h and 24 h after fasted and fed conditions, respectively. C_max (fasted, 1.34 ± 0.12 ㎍/mL; fed, 2.47 ± 0.19 ㎍/mL) and T_max (fasted, 3 h; fed, 10 h) differed substantially between the 2 groups. AUC and t_1/2λz were significantly higher in fed (42.46 ± 6.64 mg × h/L; 17.14 ± 4.68 h) compared to fasted (12.38 ± 1.13 mg × h/L; 6.55 ± 1.25 h) dogs. The relative oral bioavailability of TAL for the fasted group was low (36.92% ± 3.28%). Conclusions: Feeding affects the pharmacokinetics of oral TAL in dogs, showing a delayed, but higher absorption with different rate of elimination. These findings are of importance in clinical veterinary settings, and represent a starting point for further related studies.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• v.3 no.2
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• pp.103-114
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• 2022

Zoological Parks house exclusive animal species, thus creating a source of education and awareness for visitors. Big cats like tigers and leopards are among the most visited species in zoos globally. However, they often display stressful or stereotypic behaviours. Such behaviours are influenced by multiple factors including visitors, animal history, and captive environment. To understand this impact, we investigated the behavioural response of tigers and leopards to visitation, captive, and biological factors. The behaviour of eight big cats housed in the National Zoological Park, New Delhi, was monitored using focal sampling technique during May and June 2019. We recorded the captive and biological factors and visitor density for the subjects. The study revealed high proportions of inactive and stereotypic behaviours amongst the species. Tigers and leopards were found to perform stereotypic behaviours for 22% and 28% of their time, respectively. Generalised Linear Models revealed a significant variation of stereotypy in association with the factors. Stereotypy was influenced by visitor density, age, sex, breeding history, coat colour, and enclosure design. Adults, males, white-coated, previously bred, and those housed in smaller and simple enclosures display more stereotypy than young, females, normal-coated, unbred, and those housed in larger and complex enclosures, respectively. A high density of visitors induced more stereotypic behaviours amongst the big cats. As providing entertainment and awareness amongst the public is one of the fundamental objectives of the zoo, visitors can not be avoided. Thus, we suggest providing appropriate enrichments that would reduce stereotypies and promote naturalistic behaviours.