• Journal of Plant Biotechnology
• /
• v.45 no.3
• /
• pp.207-216
• /
• 2018

Solanum berthaultii is one of the wild diploid Solanum species, which is an excellent resource in potato breeding owing to its resistance to several important pathogens. On the other hand, sexual hybridization between S. berthaultii and S. tuberosum (potato) is limited because of their sexual incompatibility. Therefore, cell fusion can be used to introgress various novel traits from this wild species into the cultivated potatoes. After cell fusion, it is crucial to identify fusion products with the aid of molecular markers. In this study, the chloroplast genome sequence of S. berthaultii obtained by next-generation sequencing technology was described and compared with those of five other Solanum species to develop S. berthaultii specific markers. A total sequence length of the chloroplast genome is 155,533 bp. The structural organization of the chloroplast genome is similar to those of the five other Solanum species. Phylogenic analysis with 25 other Solanaceae species revealed that S. berthaultii is most closely located with S. tuberosum. Additional comparison of the chloroplast genome sequence with those of the five Solanum species revealed 25 SNPs specific to S. berthaultii. Based on these SNPs, six PCR-based markers for differentiating S. berthaultii from other Solanum species were developed. These markers will facilitate the selection of fusion products and accelerate potato breeding using S. berthaultii.

• International Journal of Industrial Entomology and Biomaterials
• /
• v.4 no.2
• /
• pp.121-126
• /
• 2002

Breeding programme was initiated during November, 1995 with the main objective to breed productive multivoltine breeds/ hybrids with suitable genetic constitution to suit the fluctuating tropical climate prevailed in India. Two multivoltine breeds viz., BL-24 and BL-27 selected were as breeding resource materials from the silkworm germplasm maintained at Central Sericul-tural Research and Training Institute, Mysore. By adopting hybridization, backcrossing inbreeding and selection, a new multivoltine breed namely BL-67. This breed spins light greenish yellow cocoons and cocoon shape is oval with medium to coarse grains. The evolved breed was crossed with five tropical bivoltine breeds viz., NB4D2, CSR2, CSR5, CSR18 and CSR101 to study the combining ability, and identified a superior hybrid, BL67 ${\times}$ CSR101, named as CAUVERY, The hybrid is characterized by high pupation rate (>95%), high shell weight (> 35 cg), high cocoon shell ratio (> 20%), longer filament length (> 900 m) and high neatness (93) with a renditta of 6.5 producing 2A-3A grade silk. The hybrid is selected for Race Autho-rization test of Central Silk Board.

• Journal of Animal Environmental Science
• /
• v.20 no.4
• /
• pp.177-182
• /
• 2014

This research was conducted to grasp the present condition of environmental management relating to Korea detector dogs that performed their duties such as detection and lifesaving. We directly conducted a survey throughout visit to five government agencies. In environmental management case, average length and width of kennels were 1.78 m and 3.50 m, respectively. Types of floor were searched as coating cement, tile, ucrete and concrete. Most of government agencies ventilated kennels using ventilation fan systems with natural ventilation. But there were differences between government agencies in frequencies of the sanitary management items. On the other side, major diseases of the German shepherd breed as a major detector dog were in order of arthritis, measles, earache, and dermatosis. And their daily training time was average 4 hours. Finally, it is thought that these results could be used as a basic data of standard breeding management manual of Korea detector dog.

• Journal of Embryo Transfer
• /
• v.22 no.4
• /
• pp.209-212
• /
• 2007

This study was performed to establish the early pregnancy diagnosis with evaluation of size of embryonic vesicles and change of crown-rump length (CRL) by transrectal ultrasonography in the Korean black goats. Early pregnancy diagnosis was performed in the 114 goats from 19 to 35 days after coitus by transrectal 5.0 MHz transducer. Embryonic vesicles and CRL in the 142 goats from 19 to 42 days was measured to establish the correlation between gestational age and these parameters. The early pregnancy diagnosis achieved 80% accuracy after days 25 and 100% accuracy after days 28 after breeding. Diameters of embryonic vesicle and CRL were significantly increased with the gestational age. In these facts, early pregnancy diagnosis is possible on Days 25 after breeding and measurement of embryonic vesicles and CRL were useful to predict the gestational age in Korean black goat.

• International Journal of Industrial Entomology and Biomaterials
• /
• v.14 no.2
• /
• pp.93-97
• /
• 2007

A Silkworm breeding programme was designed to develop a robust but productive bivoltine silkworm hybrid of Bombyx mori L. suitable for rearing throughout the year in tropical climate by utilizing indigenous polyvoltine and productive bivoltine breeds. The breeding was carried out under high temperature ($36^{\circ}C{\pm}1^{\circ}C$) and low humidity ($50{\pm}5%$) conditions in the environmental chamber. By $F_{12}$, three oval and three dumbbell breeds were isolated with higher survival and productive merits. These breeds were utilized in the hybrid evaluation along with other popular breeds. Based on combining ability test results, the hybrid $SR_2{\times}SR_5$ was selected for large scale testing and evaluated in different seasons. The evaluation studies indicated that the hybrid has higher viability and productive merits and it is suitable to rear throughout the year. The hybrid $SR_2{\times}SR_5$ recorded a survival of 92.0%, cocoon shell weight of 0.417 g, cocoon shell percentage of 23.0 and a filament length of 1042 meters under hot and dry conditions of environmental chamber compared to the control thermo-tolerant hybrid $CSR18{\times}CSR19$.

• Korean Journal of Breeding Science
• /
• v.41 no.1
• /
• pp.9-15
• /
• 2009

Recently, giant embryonic rice and functional rice food are preferred by more consumers, which are attributed to the fact that the embryo has high concentrations of essential amino acids, fatty acids, and vitamins relative to other parts of rice grains. In this report, the heredity and stability of giant embryo mutations in successive generations were analyzed regarding a giant embryonic line, 'P47', induced by T-DNA insertion and a $F_2$ population from a cross between 'P47' and 'Junam'. The mutant lines with increases of 1.5, 1.7 and 1.8 times on embryo length, width and 100-embryo weight to those of the control showed stable inheritance across three generations. The continuous frequency distributions of embryo size in the $F_2$ population showed that the embryo size is a quantitative trait of polygene controlled. In addition, wide range of transgressive segregations of six traits affecting embryo size confirmed exchange of genetic materials and recombination between genes controlling embryo size. Five giant embryo mutant lines selected from the $F_2$ population will be used for artificial selection and improvement of giant embryonic varieties.

• Journal of Animal Science and Technology
• /
• v.64 no.1
• /
• pp.183-186
• /
• 2022

Lactiplantibacillus plantarum (L. plantarum) ST was isolated from De'ang pickled tea in Yunnan Province, China. The genomes of strain ST were fully sequenced and analyzed using the PacBio RS II sequencing system. Our previous study has shown that L. plantarum ST is a potential probiotic strain. It had strong tolerance in the simulated artificial gastrointestinal tract, and in the antagonism tests, this strain showed strong antibacterial activity. Therefore, as a probiotic, it may be used in animal breeding. L. plantarum ST genome was composed of 1 circular chromosome and 7 plasmids. The length of the whole genome was 3320817 bp, and the annular chromosome size was 3058984 bp, guanine + cytosine (G ± C) content (%) was 44.76%, which contained 2945 protein-coding sequences (CDS). This study will contribute to a further comprehensive understanding of L. Plantarum ST at the genomic level and provide a theoretical basis for its future application in animal breeding.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2022.10a
• /
• pp.293-293
• /
• 2022

This study is to develop new wheat breeding material through wide hybridization with wild species harboring useful characteristics such as salt, heat, and drought tolerance. Leymus mollis, wild rye was used to improve wheat genetic quality. L. mollis, is a perennial plant harboring tolerance against salt, heat, and drought because L. mollis distributes on the seaside. The F₁ hybrids were produced by crossing between common wheat (Triticum aestivum L., Chinese Spring) and L. mollis. Genomic in situ hybridization revealed that the F₁ hybrids have L. mollis genome. For the evaluation of salt and drought tolerance, seeds from the F₂ were used. Under 2% NaCl solution, the F₃ wheat-Leymus addition plants with salt tolerance showed more tillering and longer roots than other F₃ plants without salt tolerance. Also, the F₃ plants with salt tolerance showed better shallow-rooted than other F₃ plants without salt tolerance. Finally, the F₃ plants with salt tolerance made seed-setting under 2% NaCl condition, but other F₃ plants without salt tolerance were not. Under drought conditions, the F₃ plants with drought tolerance showed longer culm and spike length than other F₃ plants without drought tolerance and even those of Chinese Spring under well-water conditions. We evaluated and selected the F₃ plants with salt or drought tolerance for generation advancement.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2022.10a
• /
• pp.276-276
• /
• 2022

Wheat is one of the most widely grown food crops worldwide. Extreme precipitation and wind disturbances increased due to the abnormal climate, which resulted in increased lodging. Introduction of dwarf genes in wheat significantly increased lodging resistance and productivity in wheat breeding. In this study, we performed the genotyping of dwarfing genes between 'Keumkang' and 'Komac 5' ('Keumkang' mutant). In addition, we investigated the relationship between plant height and several phenotypic characters using F₂ segregation populations derived from crosses between the two varieties. There was no significant difference in phenotypic characters between the two varieties except for plant height. In the genotyping analysis using dwarfing genes, mutations of two dwarfing gene were found to be induced between the two varieties. The four genotypes of the F₂ populations from a crossing between 'Keumkang' and 'Komac 5' were used to compare and evaluate the effects of two dwarfing genes. Plants with two single mutant dwarfing gene and double mutant dwarfing gene revealed reduced plant heights than control plants by 4.5%, 6.9%, and 33.2%, respectively. The phenotype analysis showed that double mutant dwarfing gene affected wheat stem growth as the length decreases from the second node, resulting in decreased plant height. However, there were no significant differences in the agronomic traits between mutant plants and control plant. These results may provide novel information about the effect of double mutant dwarfing gene on plant height, and may help improve lodging tolerance and wheat yield.

• Asian-Australasian Journal of Animal Sciences
• /
• v.20 no.11
• /
• pp.1651-1654
• /
• 2007

The mouse myxovirus resistance protein 1 (Mx1) is known to be sufficient to confer resistance to influenza viruses, and the gene encoding Mx1 is, therefore, an interesting candidate gene for disease resistance in farm animals. The porcine Mx1 gene has already been identified and characterized based on its homology with mouse Mx1; the full-length coding region of the pig Mx1 gene spans 2,545 bp (M65087) and is organized into 17 exons compared with the human ortholog mRNA. In this study, the exons 9, 10 and 11 and introns 6 and 9 of the porcine Mx1 gene were cloned and sequenced. Two SNPs were identified in exons 9, 10 and 11 but none of the SNPs led to an amino acid exchange, and the other eleven variants were detected in introns 6 and 9, respectively. Differences in allele frequency between Meishan and other pig breeds were observed within intron 6, of which an $A{\rightarrow}G$ substitution at position 371 was detected as an SnaBI PCR-RFLP. The association analysis using the Large White${\times}$Meishan $F_2$ offspring suggested that the Mx1 genotype was associated with variation in several immunity traits that are of interest in pig breeding. However, further investigations in more populations are needed to confirm the above result.