• International Journal of Industrial Entomology and Biomaterials
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• v.46 no.2
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• pp.55-59
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• 2023

One of the necessary conditions for the mass production of the edible insect, the white-spotted flower chafer Protaetia brevitarsis seulensis (Kolbe), is to breed a strain with excellent egg-laying ability. To identify external morphological traits related to egg-laying ability, we investigated the effects of the weight, length, and width of female adults on egg production. Correlation and multiple regression analyses revealed a positive correlation between the weight of female adults and egg production. This study suggests that selection of heavier females is a good strategy for breeding strains with superior egg-laying characteristics. The results of this study will serve as important foundational data for future breeding of superior strains.

• Journal of Aquaculture
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• v.21 no.4
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• pp.317-324
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• 2008

This study estimated the genetic parameters and breeding values for the growth-related traits of the 1st generation produced in 2005. The heritability of body weight, total length, body height, body shape and condition factor of 180 days old olive flounders Paralichthys olivaceus, the 1st generation of selection, was estimated as 0.564, 0.590, 0.588, 0.306 and 0.332, respectively. And reproductivity of genetic evaluation for crossing superior flounders and inferior ones was tested using the subsequent generation produced in 2006 based on the breeding values of 1st generation. In the least-squares means of body weight and total length for each group of crossing, the values of crossing group between superior flounders ($S{\times}S$) showed $145.6{\pm}1.8\;g$ and $22.4{\pm}0.1\;cm$, respectively. The values of crossing group between superior and inferior flounders ($S{\times}I$) showed $133.2{\pm}2.5\;g$ and $22.1{\pm}0.1\;cm$, respectively. The values of crossing group between inferior flounders ($I{\times}I$) showed $114.0{\pm}2.1\;g$ and $21.08{\pm}0.12\;cm$, respectively. In the results, flounders are determined as appropriate selective breeding fish with the high heritability of flounders in early ages at 180 days old, and the reproductivity of genetic evaluation was also high.

• Asian-Australasian Journal of Animal Sciences
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• v.10 no.2
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• pp.240-244
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• 1997

This study was conducted in North Sulawesi, Indonesia to identify the factors affecting reproductive efficiency and to determine the contribution of each factor in reproductive efficiency of Indonesian native cows. Records of reproductive efficiency, body size (hip height, body weight, body length, heart girth) as well as age on one hundred and sixty seven Indonesian native cows (seventy six cows of the Bali breed and ninety one cows of the synthetic breed from unknown proportion of th Madura, the Sumba and the Ongole breeds) of 58 farmers were available in this research. The data were analyzed by covariance analysis using SAS package including reproductive efficiency as a dependent variable and breed, body size (hip height, body weight, body length, heart girth) and age of cows as independent variables. The contribution of each independent factor to dependent variable was estimated as the percentage of the sum of square in the corrected total sum of squares. The results showed that body weight and body size of cows would be considered as th most important factors affecting reproductive efficiency of Indonesian native cows. Therefore, the reproductive efficiency could be increased by 1) using cows that reached a target weight of at least 250 kg, 2) using cows with relatively well developed height at hip of at least 117 cm, heart girth of at least 150 cm, and body length of at least 125 cm, 3) keeping breeding cows that reach a target age of 24 months old with the maximum interval between postpartum and next breeding season of 90 days for each year, and 4) using good nutritional feeding to reach the optimum weight and body condition.

• Journal of Korean Society of Forest Science
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• v.96 no.2
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• pp.178-182
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• 2007

Rauvolfia serpentina (L.) Benth. ex Kurz is a medicinal plant and an endangered tropical rainforest plant species. Since the field cultivation that aims to fulfill the industrial needs is never accomplished, tissue culture appears to be the most feasible way to improve the quality and quantity of R. serpentina. This experiment used two kinds of explants (roots and shoots) to induce optimal root formation in different combinations of auxin and photoperiod. Each explants exhibited different responses on given treatments. Differentiated root could be produced from explants cultured in IBA 20 mg/L with and without light. The highest number of roots, root length and root weight induced from shoot explants were effective on MS medium containing IBA 20 mg/L and incubated under dark condition, while highest total weight (callus and root) from root explants cultured on MS medium supplemented 10 mg/L IBA and 10 mg/L NAA and incubated under day length (11/13 hr). The root induced from shoot explants produced the highest major alkaloid content. The highest content of ajmaline (2.17 ppm fresh weight) and reserpine (1.30 ppm fresh weight) were observed in shoot explants cultured in MS medium containing combination of IBA 10 mg/L and NAA 10 mg/L and incubated under dark condition, yohimbine (1.47 ppm fresh weight) was in the shoot explants cultured in MS medium containing NAA 20 mg/L and incubated under day length, while serpentine was absent.

• Korean Journal of Breeding Science
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• v.41 no.3
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• pp.279-283
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• 2009

A new yellow waxy corn hybrid "Daehakchal Gold 1" was developed from single cross between Yeongdeok Jaera and Okchen Jaera at Chungnam National University in 2007. Inbred CNU57 derived from Yeongdeok Jaera was used as the seed parent of Daehakchal Gold 1, and inbred CNU 27 derived from Okchen Jaera as the pollen parent. Tasseling date of this hybrid was seven day later than that of check hybrid, Chalok 1. Daehakchal Gold 1 was 19.7cm in ear length and 4.5cm in ear diameter. The yield of Daehakchal Gold 1 and check hybrid in dry matter were 146.5g and 113.9g per plant, respectively. On yield trial, which were increased 29.7% compared with a check hybrid, Chalok 1. Especially, Daehakchal Gold 1 had yellow kernels and good eating quality. The ratio of kernel set length/ear length was similar to Chalok 1. It is moderately resistant to Bioporalis maydis and corn borer. The yields of Daehakchal Gold 1 in fresh ear weight and in number of fresh ear were 16% and 8%, respectively, higher than those of a check hybrid in regional yield trials for three years. Seed production of this hybrid has gone well due to good match during crossing between seed and pollen parents.

• Korean Journal of Breeding Science
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• v.42 no.5
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• pp.470-480
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• 2010

The objectives of this study were to identify QTLs for agronomic traits using introgression lines from a cross between a japonica weedy rice and a Tongil-type rice. A total of 75 introgression lines developed in the Tongil-type rice were characterized. A total of 368 introgressed segments including 285 homozygous and 83 heterozygous loci were detected on 12 chromosomes based on the genotypes of 136 SSR markers. Each of 75 introgression lines contained 0-9 homozygous and 0-8 heterozygous introgressed segments with an average of 5.8 segments per line. A total of 31 quantitative and 2 qualitative loci were identified for 14 agronomic traits and each QTL explained 4.1% to 76.6% of the phenotypic variance. Some QTLs were clustered in a few chromosomal regions. A first cluster was located near RM315 and RM472 on chromosome 1 with QTLs for 1,000 grain weight, culm length, grain width and thickness. Another cluster was detected with four QTLs for 1,000 grain weight, grain length, grain width and grain length/width ratio near the SSR marker RM249 on chromosome 5. Among the 31 QTLs, 9 (28.1%) Hapcheonaengmi3 alleles were beneficial in the Milyang23 background. ILs would be useful to confirm QTLs putatively detected in a primary mapping population for complex traits and serve as a starting point for map-based cloning of the QTLs. Additional backcrosses are being made to purify nearly isogenic lines (NILs) harboring a few favorable Hapcheonaengmi3 alleles in Milyang23 background.

• Fisheries and Aquatic Sciences
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• v.27 no.2
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• pp.100-110
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• 2024

Biometric evaluation and reproductive biology of Oreochromis niloticus were studied from Geray Reservoir. Between November 2021 and August 2022, 316 O. niloticus specimens were collected from this reservoir. The observed male to female sex ratio was 1:1.14, which was statistically insignificant compared with the hypothetical 1:1 sex ratio (X² = 1.27, p > 0.05). The length-weight relationship was curvilinear with allometric growth (b < 3) and statistically significant from the expected isometric growth pattern (b = 3). The regression equation for females was TW = 0.0264TL^2.72 (r² = 0.90, n = 168), males (TW = 0.0183TL^2.79, r² = 0.93, n = 148), and combined sexes (TW = 0.0229TL^2.76, r² = 0.91, n = 316). Gonadosomatic index (GSI) ranged from 0.8 to 1.4 (males) and 1.8 to 4.9 (females). The breeding season lasted from March to June, with a GSI peak in April. Fecundity ranged from 279 to 1,528 eggs./fish, with a mean of 1,025 eggs./fish. The correlation between absolute fecundity and length was more significant (F = 0.76TL^2.8, R² = 0.94, p < 0.05) than with weight (F = 17.683TW^0.71, R² = 0.92, p < 0.05). This study provides basic information on the biology of O. niloticus in the Geray Reservoir for future fishery management tools for the conservation and utilization of this valuable freshwater fish.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.4
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• pp.449-456
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• 2011

Eighteen different biometric traits in 407 Kankrej cows from their breeding zone, i.e. Palanpur district of Gujarat, India, were recorded and analyzed by factor analysis to explain body conformation. The averages of body length, height at withers, height at shoulder, height at knee, heart girth, paunch girth, face length, face width, horn length, horn diameter, distance between horns, ear length, ear width, neck length, neck diameter, tail length with switch, tail length without switch and distance between hip bones were $123.44{\pm}0.37$, $124.49{\pm}0.28$, $94.68{\pm}0.30$, $38.2{\pm}0.14$, $162.56{\pm}0.56$, $178.95{\pm}0.70$, $44.09{\pm}0.10$, $15.91{\pm}0.05$, $42.47{\pm}0.53$, $26.07{\pm}0.19$, $13.34{\pm}0.08$, $31.24{\pm}0.12$, $16.10{\pm}0.05$, $50.63{\pm}0.18$, $73.21{\pm}0.32$, $111.62{\pm}0.53$, $89.34{\pm}0.34$ and $17.28{\pm}0.10\;cm$, respectively. The correlation coefficients between different traits ranged from -0.806 (horn diameter and distance between horns) to 0.815 (heart girth and paunch girth). Most of the correlations were positive and significant. Factor analysis with promax rotation with power 3 revealed three factors which explained about 66.02% of the total variation. Factor 1 described the cow body and explained 38.89% of total variation. The second factor described the front view/face of the cow and explained 19.68% of total variation. The third factor described the back of the cow and explained 7.44% of total variation. It was necessary to include some more variables for factor 3 to obtain a reliable estimate of the back view of the cow. The lower communities shown for distance between horns, horn diameter, ear width and neck diameter indicated that these traits did not contribute effectively to explaining body conformation and can be dropped from recording, whereas all other traits are important and needed to explain body conformation in Kankrej cows. The result suggests that principal component analysis (PCA) could be used in breeding programs with a drastic reduction in the number of biometric traits to be recorded to explain body conformation.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.6
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• pp.835-841
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• 2018

Objective: The aim of this study was to investigate the reversibility and safety of KISS1 metastasis suppressor (KISS1) gene vaccine in immunocastration. Methods: Six eight-week old ram lambs were randomly divided into vaccinated and control groups. The vaccine (1 mg/ram lamb) was injected at weeks 0, 3, and 6 of the study. Blood samples were collected from the jugular vein before primary immunization and at weeks 2, 4, 6, 10, 14, 22, and 30 after primary immunization. All ram lambs were slaughtered at 38 weeks of age, and samples were collected. Results: The specific anti-KISS1 antibody titers in vaccinated animals were significantly higher and the serum testosterone level was significantly lower than those in the control groups from week 4 to 14 after primary immunization (p<0.05). No significant difference was observed at weeks 22 and 30 after the primary immunization. Similar results were also found for scrotal circumference, testicular weight, length, breadth, and spermatogenesis in seminiferous tubules in week 30 after primary immunization. KS (KISS1-hepatitis B surface antigen S) fusion fragment of KISS1 gene vaccine was not detected in host cell genomic DNA of 9 tissues of the vaccinated ram lambs by polymerase chain reaction. Conclusion: The effects of KISS1 gene vaccine in immunocastration were reversible and no integration events were recorded.

• Journal of Ecology and Environment
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• v.30 no.4
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• pp.287-292
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• 2007

We monitored a breeding population of the Asian toad (Bufo gargarizans), in the Wonheunge pond at Sannamdong, Chungju, from 5 March to 11 April, 2006 and 14 February to 31 March, 2007 to investigate their movement patterns, breeding population sizes, and physical characteristics. Terrestrial migration to the pond started on 5 March in 2006 and 14 February in 2007. We captured a total of 266 immigrating individuals (213 males, 53 females) in 2006 and 307 (222 males, 85 females) in 2007, and found 50 adults apparently killed by motor vehicles while migrating to the pond in 2007. Emigration from the pond to terrestrial sites started on 15 March 2006 and 5 March 2007. We captured a total of 245 emigrating toads (181 males, 65 females) in 2006 and 99 (92 males, 7 females) in 2007. An additional 10 emigrating adults were found dead on the road. During both the immigration and emigration periods, two peaks in capture frequency appeared for each sex in each breeding season. The immigration peaks corresponded with higher temperatures, while the emigration peaks corresponded with high humidity. Migrating Asian toads showed sexual size dimorphism and a male-biased sex ratio. Body weights and SVL (snout-vent length) of immigrating and emigrating individuals were negatively related with migration dates.