• 고려인삼학회:학술대회논문집
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• 고려인삼학회 2004년도 춘계 총회 및 학술대회
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• pp.17-28
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• 2004

현재 재배되고 있는 고려인삼은 혼계상태로서 개체간의 형질변이가 대단히 심하며, 종자채취를 4년 후에 하기 때문에 신품종육성을 위해서는 매우 오랜 기간이 필요하다. 그러나 식물형질전환기술의 발달로 목적하는 유전자를 식물체에 재도입하여 새로운 품종을 육성할 수 있는 기술이 보급되어 유용유전자만 있다면 단시간에 고기능성 신품종을 육성할 수 있을 것이다. 본 과제에서는 인삼의 유용유전자를 대량으로 발굴하기 위하여 인삼의 조직별, 종간 및 처리간에 의한 cDNA library 총 10종 이상을 제작하고 이들 cDNA library로부터 인삼의 유용유전자원을 확보하기 위하여 EST 20,000개를 5' 한쪽 방향에서 분석하고 이들 분석된 EST clone의 데이터는 data base화 하여 많은 연구자들이 연구정보를 공유한 수 있게 하였다. 그리고 EST clone 중에서 완전한 단백질의 유전정보를 포함하고 있는 clone을100개 선발하여 전장의 염기서열(full length sequence)을 분석하고 data base를 구축하고parental clone을 선발하여 cDNA chip을 제작하였다. 특히 257 clone 주에서 기능성 및 내재해성 유용유전자를 선발하여 전염기서열분석(full length sequencing)을 한 후 인상에 재도입하여 고기능성 및 내재해성 인삼의 분자육종을 비교적 단시간내에 할 수 있는 형질전환 및 재분화 시스템을 개발하고 토양순화 시스템을 확립하고자 하였다.

• The Korean Journal of Ecology
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• 제8권2호
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• pp.119-126
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• 1985

One nest of the Common Kingfisher Alcedo atthis bengalensis Gmelin breeding in the area of Mt. Bangu, Mugye-ri, Jangye-ri, Jangyu-myon, Gimhae-gun, Gyongnam province, was observed for 43 days from July 18 to August 29, 1984. The nest was found at a height of 71cm in a steep mountain side. It was tunnel-shaped, with a size of 53cm(length), 5.5*6.4cm (the diameter of entrance) and 16.1*10.5cm (the diameter of incubation seat). The cluth size was 5 eggs. The eggs were white in color, the average size of 5 eggs being 20mm (length)*17.9mm(width). The incubation period was 18 days, the parent birds stayed in the nest at on average of 494 minutes and the rate of incubation was 82.20%. The incubation time increased gradually. The nesting period of 3 chicks was 25 days, and the average feeding frequency was 5, 50 times per day with the highest frequency of 10 times on the 14th days after hatching. The time and frequency of attendance gradually decreased as their chicks grew up. Total increase length in body was; body length 45.35longrightarrow147.80mm, wing 6.95longrightarrow63.40mm, bill 1.73longrightarrow29.36mm and body weight 2.93longrightarrow41.00gr. The prey for the 3 chicks of the nest was entirely fish. The main food item was the fry of Gobiobotidae. The home range of the nest was 0.43ha. and the territory size was 0.05ha. Their feeding ground were outside of their territory.

• Journal of Plant Biotechnology
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• 제45권2호
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• pp.140-145
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• 2018

Inducing genetic and morphological variation through conventional method is very difficult. Therefore, mutation induction through in vitro technology brings numerous advantages over the conventional breeding. Thus, the individual shoots (1 ~ 2 cm) were irradiated with gamma rays (10 ~ 70 Gy). The result revealed that the explants treated with higher doses (40, 50, 60, and 70 Gy) showed deleterious effects of ionizing radiation. The highest survival rate among ${\gamma}$ treated explants recorded was 71% in 10 Gy treatments while the lowest survivality was 15% in 70 Gy. Lethal dose 50% ($LD_{50}$) dose was found to be 33 Gy. In the in vitro condition, rooting reponse showed that increase in gamma irradiation dose resulted in the inhibition of root growth. Meanwhile, non-treated explants had the best rooting ability with the maximum number of root per explant (20) within a short period of time (6 days), with the highest root length of (15.1 cm). The longer period in rooting (12 days) and lowest number of root per explant (8) with shortest root length (10.1 cm) were recorded at 30 Gy treatment. The highest shoot length (13.6 cm) was observed at control treatment and the shortest shoot length (10.4 cm) was observed at 30 Gy. In the nursery, lowest leaf number (5) was observed at 30 Gy compared with other treatments. The highest chlorophyll content (49.8) was recorded at 10 Gy treated seedling. Irradiated explants with 10 Gy found to be superior over the control treatment and had positive effects in main growth parameters such as chlorophyll content.

• International Journal of Industrial Entomology and Biomaterials
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• 제6권2호
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• pp.171-178
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• 2003

Four thermo-tolerant lines of silkworm, Bombyx mori, (L.) viz., A HT, B HT (Chinese type) and F HT, G HT (Japanese type) were evolved by utilizing the breeding resource material (identified from initial screening at a temperature of 31 ${\pm} 1^{\circ}C$ and relative humidity 85 ${\pm}$ 5%) through conventional breeding. These tolerant lines were crossed with productive breeds and forty four hybrids were evaluated on eight economic traits by the Multiple Trait Evaluation Index Method. Ten hybrids were short-listed based on the average evaluation index value larger than 50 for eight economic traits studied. The identified ten hybrids recorded higher index values (＞ 50) for most of the traits studied. Single hybrid G ${\times}$ CSR12 indicated average index value larger than 50 for six traits viz., pupation number (58), cocoon weight (67), shell weight (65), average filament length (74), raw silk % (69), reelability % (51) except for shell ratio % (41). The standard deviation of the cocoons in the above hybrid was 8.41 in the hybrid cocoon length and width measurement. However, two selected hybrids viz., A ${\times}$ CSR5 and G ${\times}$ CSR13 recorded average index value larger than 50 for all the traits viz., pupation number (57, 60), cocoon weight (50, 54), shell weight (56, 57), shell ratio percentage (59, 53), average filament length (55, 60), raw silk percentage (63, 67) and reelability percentage (53, 53). The standard deviation of the cocoons in the two selected hybrids viz., A ${\times}$ CSR5 and G ${\times}$ CSR13 was 8.41 and 8.06 respectively in the cocoon length and width measurement.

• 한국연초학회지
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• 제11권2호
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• pp.181-195
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• 1989

The study was conducted to obtain the genetic informations for some useful characters in tobacco breeding. The eight parents and a set of 28 crosses of F1's and F2's were used as materials, and planted on two different cultivated systems, i. e., oriental's and burley systems, during 1986-1988 at taegu Experiment Station, Korea Ginseng & Tobacco Research Institute. The observed characters were six agronomic characters which were plant height, number of leaves per plant, leaf length, leaf width, days to flowering, yield and five chemical components, nicotine, total nitrogen, protein nitrogen, total volatile base and pet, ether extract. The results obtaining are summarized as follows: 1. The higher heritabilities were found for days to flowering yield and nicotine in both generations, but values for number of leaves per plant, leaf length, total nitrogen and protein nitrogen were lower than other characters. 2. Genotypic correlation coefficients among all pairs of characters were slightly higher than the corresponding phenotypic correlations in F1's and F2's on two different cultivated systems. The relationship between leaf length and leaf width was the positive correlation, but that between number of leaves per plant and leaf width was negative. 3. From the genotypic correlations between yield and other characters, a conclusion that the yield was highly correlated with plant height, leaf length, leaf width and days to flowering was given. 4. Quality was positively correlated with number of leaves per plant and nicotine, but negatively with the other agronomic characters and chemical components.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.279-279
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• 2022

Rice is 34% of the world's population used as a staple food. But the world population is increasing. Food security is not well protected. Improving cultivar development can address food security. Quantitative trait locus (QTL) mapping is a statistical analysis using both phenotypic and genotypic dates. The purpose of QTL mapping is to determine a gene. Increasing grain size is a way to increase yield in rice. Grain size-related genes were mapped using CNDH population obtained by cross-breeding Cheongcheong (Indica) and Nagdong (Japonica) through anther culture. Grain harvested from experimental field of Kyungpook National University in Gunwi in 2021. Genes related to grain length were detected between RM5964-RM12285, RM20924-RM20967 in chromosome 1, 7. LOD score is 5.88 and 5.6. Genes related to grain width was detected between RM289-RM18130 in chromosome 5. LOD score is 4.57. Genes related to grain length/width ratio were detected between RM5459-RM3482, RM5699-RM1211 and RM3838-RM3381 in chromosome 1, 2, 5. LOD score is 3.75, 3.14 and 3.41. 4 genes was detected in chromosome 1 and 2 genes was detected in chromosome 2 and 7 genes was detected in chromosome 5. 2 genes related to grain shape and quality were detected. 4 genes related to grain length were detected. 4 genes related to grain size were detected. 1 gene related to grain size and weight was detected. 2 genes related to grain length and weight were detected. By finding the gene related to grain size, it provides food to people threatened by food security and solves the food shortage.

• 생명과학회지
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• 제14권5호
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• pp.821-828
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• 2004

엽면적과 엽장 및 엽폭은 식물의 광합성 효율과 관련이 있다. 단위 엽면적당 광합성율을 증가는 콩에서 종실 수량을 증가시킨다 따라서 본 연구는 큰올콩과 신팔달콩 및 익산10호를 각각 교배하여 얻은 두 집단이 잎의 엽장과 엽폭 및 장폭비를 확인할 수 있는 SSR 마커를 선발하기 위하여 실시하였다. 잎의 장폭비는 두 집단에서 엽폭과 유의적인 부의 상관을 보였다. 엽장은 큰올콩/신팔달롱 조합에서 연관군 DIb+W와 L에서 두개의 작은 양적 형 질 유전자좌 (QTL)를 탐색하였으며, 큰올콩/익산10호 조합에서는 연관군 1와 L에서 두개 의 양적 형 질 유전자좌가 관련하였다. 엽폭은 큰올콩/신팔달콩 조합에서 2개, 큰올콩/익산10호 조합에서 3개의 양적형질 유전자좌가 관련하였으며 이들은 각각 전체 형질 변이의 13% 및 18.04%를 설명할 수 있었다. 장폭비는 큰올콩/신팔달콩 조합에서 연관군 I와 L에서 2개, 큰올콩/익산10호 조합에서 연관군 Cl과 E 및 L에서 3개의 양적형질 유전자좌가 관련하였다.

• 한국연초학회지
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• 제9권1호
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• pp.45-55
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• 1987

For some basic information in tobacco breeding, the modes of inheritance and heritabilities for the twelve agronomic and chemical characters were estimated in the study of eight varieties partial diallel set. Additive gene actions were significant for all characters except total nitrogen content and dominance gene effects were also significant for all characters. Yield, number of leaves per plant, leaf length and leaf shape index were inherited as partial dominance, and overdominance was detected for plant height, stem diameter, internode length, total alkaloid, total nitrogen and total sugar content. Dominant gene showed increasing effects in yield, plant height, stem diameter, internode length, leaf width and total sugar content, but showed decreasing effects in the other characters. The broad heritability was very high for all characters. and the narrow heritability was high in days to flower and yield, but low in internode length, total alkaloid, total nitrogen and total sugar content.

• 한국동물학회지
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• 제33권3호
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• pp.260-265
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• 1990

강원도 강릉 남대천과 옥계 수림천에서 채집된 가시고기와 잔가시고기를 대상으로 외부형태의 성적 이형을 조사하였다. 조사된 형질 중 가장 뚜렷한 성적 이형을 나타내는 것은 체장에 대한 두장의 비율로서, 수컷의 두장이 암컷의 것보다 더 긴 것으로 나타났다. 그 밖에 문장 / 체장, 항문부 체고 / 체장의 비율에서도 암수간의 차이를 나타내는 경향이 있었다. 이들 어류에서 두장의 성적 이형이 나타나는 원인을 동물행동학적인 면에서 논의하였다.

• Plant Resources
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• 제3권1호
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• pp.71-78
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• 2000

Autotoxicity restricts reseeding of new alfalfa(Medicago sativa L.) after alfalfa until autotoxic chemical(s) breaks down or is dispersed into external environments, often requiring up to a year or more. One solution for reducing autotoxicity would be to select germplasms or cultivars with tolerance to the autotoxic chemical(5) and use genetically breeding program. Bioassay of seed germination and early seedling growth was conducted to evaluate autotoxic responses of 3 varieties of alfalfa to the water-soluble extracts(at 4 and 8g/L) from alfalfa ‘Cody’leaf by using agar and filter paper medium in a petri-dish assay. Root length at 5 days after seeding was more sensitive to the extract than was hypocotyl length or seed germination, and was a better parameter of autotoxic effects of alfalfa leaf extracts. Use of an agar medium gave better sensitivity of root length than did use of filter paper. Evaluating tolerance with percent of control was more important indicator than was mean of root length because of significant variation among varieties in root length of control treatment. Bioassay ranked varieties in the following order of tolerance on the basis of relative root length; “Cody” >“ Pioneer 5373” >“ Alfagraze”. Seedling growth from old “Cody” seed was more sensitive to the autotoxic chemical(5) than was that from newly produced seed.