• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.96-96
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• 2017

Rice is a staple food for nearly half of the world's population, with more than 10,000 rice varieties providing almost one-quarter of the global per capita dietary energy supply. Grain size, panicle size and branch number, grain number in a panicle are directly associated with rice productivity. Recently several genes which increase grain yield were identified through map-based cloning. Gn1a, Cytokinin oxidase, is a major grain number QTL and regulates grain number per panicle. Dep1 increases panicle branching and reduced rachis length. SCM2 (APO1) was identified by a QTL for culm strength and increased spikelet number. OsSPL16 (GW8) controls grain size and shape and then increases 1000-weight of seed. In here, to identify genotype of genes related to yield in 400 of rice germplasms possessed in National Institute of Crop Science, we had first chosen 4 of well-known genes related to yield; Gn1a, Dep1, SCM2, and OsSPL16. Among these germplasms, 195, 382, 165, and 353 of germplasms harbored the dominant type of Gn1a, Dep1, SCM2, and OsSPL16, respectively. We grouped these germplasms into a total of 10 groups using genotypes of Gn1a, Dep1, SCM2 and OsSPL16. Most rice germplasms belong to group 1, harbored Gn1a, dep1, gw8 and APO1, and group 10, harbored gn1a, Dep1, GW8 and apo1. Hanareum2 is the highest productive cultivar in Korea but do not have dominant type OsSPL16, so belong to group 1. On the other hand, in the case of Unkwang, belongs to group 10, which has dominant type of OsSPL16 but do not have the remaining genes. We can grasp the differences in rice germplasms through the Profiling of genes related to these grain yield, which will be useful for cross-breeding to integrate grain yield genes. We are continuously observing the genotype and phenotype of rice that possesses grain yield genes.

• Horticultural Science & Technology
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• v.30 no.2
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• pp.224-228
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• 2012

A new $Saxifraga$ $fortunei$ cultivar 'Crystal' was bred by crossing 'Dotaerang' with light pink and light Green (RP 58D) colored petals and 'Rocksun' with light pink and light green white (RP62A + GW157A) colored petals. The selection and flower characterization were conducted from 2004 to 2007 in a greenhouse of the National Institute of Highland Agriculture. The flowering of 'Crystal' occurred from September 21 for 31 days. The number of flowers per plant was 279.0 with many flowering habit. The flower coloration was mixed light pink and green white (RP62D + GW157B) at the opening. The number of petals were 7.0, with a width of 2.1 cm and length of 1.8 cm. The plant height was 15.3 cm and number of leaves was 133.7. 'Crystal' cultivar can be used for pot flower. Fifty percent shading is a good cultural practice against direct sunlight and soft rot disease would occurs usually by over-irrigation. 'Crystal' cultivar was registered as a new variety with No. 3466 at Korea Seed and Variety Service in April of 2011 year.

• Horticultural Science & Technology
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• v.30 no.3
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• pp.329-333
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• 2012

A new Asiatic hybrid lily 'Redsky' cultivar was selected from a cross between 'Vivaldi' cultivar with pale pink color flower as mother plant and 'Prato' with orange colored flower in 2000 year. The selected bulb line was propagated and cultivated for line nomenclature as 'Gonong-F-na-10'. The survey of the growth and flower characteristics of 'Gonong-F-na-10' were conducted from 2005 to 2007 on summer cropping system under structure in Highland Agriculture Research Center, NICS, RDA. After all 'Gonong-F-na-10' was named as 'Redsky'. The flowers of the 'Redsky' cultivar had not soft hair and fragrance, and had orange red (O-R N30D) colored petals with dark brown spots in center of flower. The stem color was dark pink and brown caused by anthocyanin pigment, the stigma and pollen colors were brown. Flowering direction was upward. The flowering date was July 7 so early flowering. The plant height was 97.2 cm so long, number of flowers was 4.9 ea, and length of flower bud was 9.3 cm. The bulb weight was about 34.8 g and its bulb size was about 14.8 cm in circumference. 'Redsky' cultivar was registered as a new variety with No. 3119 in Korea Seed and Variety Service on June of 2010.

• Journal of Animal Environmental Science
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• v.18 no.3
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• pp.151-156
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• 2012

Studies of non-cage or alternative systems have been discussing in EU because the EU has banned use of conventional cages for laying hens since 2012. This survey was carried out the information related to laying hens' welfare in Korea. Laying hens' breeding systems divided into deep litter, free range and alternative system, and livestock housings divided into windowless housing and open-type housing. The ratios of trough and bell feeder types were 37.5% and 62.5%, respectively. The ratios of nipple, trough, and bucket in waterer types 75%, 12.5%, and 12.5%, respectively. What stocking density is the most appropriate for production and welfare has been aroused. All laying hen farms met the required standards of stocking density ranged from 3.4 birds/$m^2$ to 7.6 birds/$m^2$ in this survey. According to welfare standards for laying hens, perch length should not be less than 15 cm. However, most laying hen farms did not satisfied the standards. We believe that presentdata contribute to develop alternative systems and to improve animal welfare in Korea.

• Korean Journal of Plant Tissue Culture
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• v.22 no.6
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• pp.317-322
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• 1995

As a basic research for breeding new varieties, reciprocal -intergeneric crosses between Citrus junos and P.trifoliata were made. F$_1$ hybrid production using in vitro ovule culture, gametogenesis, and fertilization phenomena were investigated. Frequency of fruit set resulting from crossing of Citrus junos and Poncirus Trifoliata was 16.6% while that of Poncirus Trifoliata and Citrus junos was 11.7%. Callus formation occurred well when ovules at the 6th week after pollination were cultured on MT (Murashige and Tucker) medium supplemented with zeatin 0.5 mg/L and NAA 1.0 or 3.0 mg/L. Immature ovules developed into mature embryos of the MT medium supplemented with 2,4-D 0.1 or 3.0 mg/L. Immature ovules developed into mature embryos of the MT medium supplemented with 2,4 D 0.1 or 0.5 mg/L. The invitro germination rates of 20-week-old ovules set C. junos $\times$ P. Trifoliata and P. Trifoliata $\times$ C. junos were 54.5% and 48.6%, respectively. The emergence ratios of trifoliate hybrids obtained by C. junos $\times$ P. Trifoliata and P. Trifoliata $\times$ C. junos were 56.7% and 100%, respectively. The chromosome number of C. junos and P. Trifoliata was n = 9 or 2n = 18, and the sizes of their pollen grain were 33.75 $\mu$ and 25.0 $\mu$. The length and width of embryo sac in C. junos and P. Trifoliata were 69.38~79.23 $\mu$ and 27.50~38.56 $\mu$, and those of egg cells were 17.50~41.50 $\mu$ and 6.25~8.12$\mu$. Fertilization of C. junos and P. trifoliata terminated 72 h after pollination.

• Journal of Mushroom
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• v.16 no.3
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• pp.186-191
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• 2018

In order to breed a new Pleurotus eryngii cultivar with a large pileus and convex shape, which are favorite traits of customers from Europe and North America, single crosses between monokaryotic mycelia derived from basidiospores of KNR2555 were performed to yield the new cultivar $2{\times}12$ chosen by cap shape (convex), diameter of pileus (60.7 mm), and quality (4.9) in a preliminary cultivation. The strain was named Gat_aeryni and was cultivated on a large scale for comparison with Kenneutari 2 ho at the GNARES and at mushroom farms. The yield of Gat_aeryni (71.7 g) was not significantly different from that of Kenneutari 2 ho (71.4 g), and the quality of the new cultivar was 6.8, which was not significantly different from that of a reference cultivar (6.5). Days for harvest, length, and diameter of the pileus in the two cultivars were statistically different by an independent t-test (P < 0.001, P < 0.05, P < 0.001 and P < 0.05 respectively). The new and reference cultivars were discriminated by PCR reactions with a primer set (URP1 and URP10) and simultaneous cultivation.

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.9
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• pp.1237-1246
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• 2013

The objective of this study was to estimate variance components and genetic parameters for lactation milk yield (LY), lactation length (LL), average milk yield per day (YD), initial milk yield (IY), peak milk yield (PY), days to peak (DP) and parameters (ln(a) and c) of the modified incomplete gamma function (MIG) in an Ethiopian multibreed dairy cattle population. The dataset was composed of 5,507 lactation records collected from 1,639 cows in three locations (Bako, Debre Zeit and Holetta) in Ethiopia from 1977 to 2010. Parameters for MIG were obtained from regression analysis of monthly test-day milk data on days in milk. The cows were purebred (Bos indicus) Boran (B) and Horro (H) and their crosses with different fractions of Friesian (F), Jersey (J) and Simmental (S). There were 23 breed groups (B, H, and their crossbreds with F, J, and S) in the population. Fixed and mixed models were used to analyse the data. The fixed model considered herd-year-season, parity and breed group as fixed effects, and residual as random. The single and two-traits mixed animal repeatability models, considered the fixed effects of herd-year-season and parity subclasses, breed as a function of cow H, F, J, and S breed fractions and general heterosis as a function of heterozygosity, and the random additive animal, permanent environment, and residual effects. For the analysis of LY, LL was added as a fixed covariate to all models. Variance components and genetic parameters were estimated using average information restricted maximum likelihood procedures. The results indicated that all traits were affected (p<0.001) by the considered fixed effects. High grade $B{\times}F$ cows (3/16B 13/16F) had the highest least squares means (LSM) for LY ($2,490{\pm}178.9kg$), IY ($10.5{\pm}0.8kg$), PY ($12.7{\pm}0.9kg$), YD ($7.6{\pm}0.55kg$) and LL ($361.4{\pm}31.2d$), while B cows had the lowest LSM values for these traits. The LSM of LY, IY, YD, and PY tended to increase from the first to the fifth parity. Single-trait analyses yielded low heritability ($0.03{\pm}0.03$ and $0.08{\pm}0.02$) and repeatability ($0.14{\pm}0.01$ to $0.24{\pm}0.02$) estimates for LL, DP and parameter c. Medium heritability ($0.21{\pm}0.03$ to $0.33{\pm}0.04$) and repeatability ($0.27{\pm}0.02$ to $0.53{\pm}0.01$) estimates were obtained for LY, IY, PY, YD and ln(a). Genetic correlations between LY, IY, PY, YD, ln(a), and LL ranged from 0.59 to 0.99. Spearman's rank correlations between sire estimated breeding values for LY, LL, IY, PY, YD, ln(a) and c were positive (0.67 to 0.99, p<0.001). These results suggested that selection for IY, PY, YD, or LY would genetically improve lactation milk yield in this Ethiopian dairy cattle population.

• Korean Journal of Environmental Biology
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• v.38 no.1
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• pp.40-46
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• 2020

Hybrid grouper(Epinephelus fuscoguttatus ♀×E. lanceolatus ♂) (mean weight 25.7±3.5g, mean length 11.2±0.9cm) were exposed to different ammonia concentrations of 0, 5, 10, 20, and 40mg L^-1 for 96 hours. The hematological hematocrit and hemoglobin parameters of the hybrid grouper were significantly decreased by 20 mg L^-1 ammonia exposure. In the organic plasma components, calcium was significantly decreased, whereas there was no change in magnesium. In the organic plasma components, the glucose and cholesterol values of the hybrid grouper were significantly increased by ammonia exposure. In the enzymatic plasma components, the ALP(Alkaline phosphatase) value of the hybrid grouper was also significantly increased by ammonia exposure. The results of this study demonstrate that acute ammonia exposure to hybrid grouper induced changes in hematological parameters and plasma components. Therefore, acute ammonia exposure over 20 mg L^-1 appears to be toxic to hybrid grouper and the results can be used as a major indicator in breeding hybrid grouper.

• Journal of The Korean Society of Grassland and Forage Science
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• v.33 no.1
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• pp.1-5
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• 2013

Andaok, a new single cross variety, is a yellow dent maize hybrid (Zea mays L.) developed by the maize breeding team at the National Institute of Crop Science (NICS), RDA in 2011. This hybrid, which has a high yield of grain, was produced by crossing two inbred lines, KS161 and KS162. KS161 is the seed parent and KS162 is the pollen parent of Andaok. Silking date of Andaok is 3 days later than that of the check hybrid, Jangdaok. Plant height of Andaok is longer than that of Jangdaok. Ear numbers per 100 plants of Andaok is more than that of Jangdaok. Ear length of Andaok is shorter than that of Jangdaok. The weight of 100 seeds of Andaok is heavier than that of Jangdaok. It has moderate resistance to southern corn leaf blight (Bipolaris maydis), black streaked dwarf virus (BSDV) and corn borer. It has strong resistance to northern corn leaf blight (Exserohilum turcicum). It has resistance to lodging. Andaok was evaluated for its yield of grain at three locations from 2009 to 2011. The grain yield of Andaok was 7.80 ton/ha. Seed production of Andaok has gone well due to a good match during crossing between the seed parent, KS161, and the pollen parent, KS162, in Yeongwol.

• Korean Journal of Medicinal Crop Science
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• v.9 no.4
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• pp.301-309
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• 2001

This study was conducted to provide the basic information on safflower collections and to identify the variations which could be utilized in safflower breeding programs. The agronomic characteristics was used to clarify the genetic relationships among safflower collections and to classify them into distint genetic groups. There were 21 early maturing collections with less than 80 days in number of days from planting to flowering. The number of primary and secondary branches ranged ${3.8{\sim}14.8\;and\;0{\sim}26.9}$, respectively, and two collections, IT201434 and IT202723, were found to be high branch types. The 101 safflower collections were classified into 11 groups based on the complete linkage cluster analysis using agronomic characteristics. The I, II, III, IV, IX, X and ? groups included the 25%, 33%, 14%, 8%, 2%, 1% and 1% of the collections, respectively. All the collections in the group III were Korean landraces. The collections in group X could be characterized as early emergence, late flowering and high yield components such as the number of capitula per plant, number of seeds per capitula and seed weight per plant. The number of capitula per plant and seed weight per plant, i.e., the two main yield components, had the highly significant positive correlations with stem diameter, number of the primary branches, number of the secondary branches, number of leaves and leaf length.