• 한국작물학회지
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• 제66권4호
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• pp.318-325
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• 2021

이 연구에서는 인공 염색체 절단의 유발원인 제부라린을 두 종류의 Leymus 염색체가 첨가된 이중 일가 외래 염색체 첨가 밀 계통의 생식세포 분열기에 처리함으로써 인공 염색체 절단이 상동성이 결여된 외래 염색체 사이의 염색체 조합에 미치는 영향을 확인하고자 수행하였다. 밀의 유전적 배경에서 두 외래 염색체의 행동은 genomic in situ hybridization을 이용하여 확인하였다. 결과적으로 생식세포분열 전기 초반에 인공 염색체 절단은 두 외래 염색체의 핵형 차이인 말단의 이질염색질을 제외한 전장에서 발생하였으며, 그로 인하여 염색체 융합이 이루어져 이가 외래 염색체의 형태가 형성되는 것을 확인하였다. 이처럼 제부라린 처리에 의한 인공 염색체 절단이 체세포분열(mitosis) 염색체뿐만 아니라 생식세포분열 염색체에 염색체 접합과 유사한 현상을 유발시키는 것을 확인하였으며, 이를 통하여 상동성을 엄격하게 조절하는 Ph1 유전자를 가지고 있는 밀의 유전적 배경에서 동조 또는 비상동 관계에 있는 염색체 사이에서 염색체 결합이 이룰 수 있는 것을 확인하였다. 반면, 인공 염색체 절단은 두 외래 염색체의 소실과 일반적인 이가 염색체의 형태가 아닌 비정상적인 형태의 외래 이가 염색체도 유발하는 것을 확인하였다. 이러한 현상은 보통 형태와 유사한 이가 외래 염색체가 형성되었음에도 불구하고 생식세포 분열의 사분자의 포자에 자매염색분체의 분포 비율에 부정적인 영향을 미침으로써 염색체 조합의 빈도를 나타내는 상동성 지수에 유의미한 차이를 나타내지 못했다. 따라서 인공 염색체 절단에 의한 염색체 결합의 빈도와 발생부위에 대한 조절을 작물학적 관점에서 이용하려면 앞으로 지속적인 염색체 연구를 바탕으로 좀 더 구체적이고 세밀한 제부라린의 투여량, 처리시간 및 처리방법에 대한 연구가 필요할 것으로 생각한다.

• 한국가금학회지
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• 제50권1호
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• pp.23-30
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• 2023

본 연구는 농장 내 계란 이송시스템에서 발생하는 충격 정도를 파악하고 파란 발생률과 비교하여 문제점을 개선하기 위해 무창계사 12개 농장을 대상으로 하여 무선 전자계란(Wireless Egg Node II)를 활용하여 조사하였다. 전체적으로 충격 크기는 세척기와 건조기 연결 부분, 컨베이어 연결부분, 트랜스퍼, 정렬기, 집란 엘리베이터, 팩커, 계란 이송 연결부분, 알받이 순으로 나타났으나 농장에 따라 충격정도 및 충격발생 지점에 많은 차이가 있었다. 계란의 충격강도와 파란 발생률은 높은 정(+)의 상관관계가 나타났으며 일회성 충격보다는 누적된 충격의 양에 따라 파각발생이 높았고, 조사 지점에 따라 5~38 G까지 다양하며 총 파각란 발생률도 3.2%~14.5%로 나타났다. 파각 발생률을 낮추기 위해서는 난각 품질이 높은 계란을 생산하고자 하는 노력도 중요하지만 난각강도가 우수해도 최종제품에 난각이 파손되는 상황을 고려할 때 실금란 발생을 초래하는 계란이송라인 개선이 절대적으로 필요한 것으로 조사되었다.

• Bulletin of the Korean Chemical Society
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• 제29권12호
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• pp.2395-2398
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• 2008

• Bulletin of the Korean Chemical Society
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• 제27권11호
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• pp.1891-1893
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• 2006

• Fibers and Polymers
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• 제4권2호
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• pp.71-76
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• 2003

We evaluated the seam characteristics with finishing, seaming and sealing processes and seam Puckering behavior of the breathable waterproof fabrics with laser scan. There were differences in 99% significant level between the seamed fabric and the sealed fabric. Seam breakage was initiated with the breakage of sewing thread, so the seam strength after seaming was almost uniform. The sewn seam strength and elongation increased with sealing process in all finishing methods. Pucker grade of laminating type was generally much higher than that of the coating type. We confirmed that wave length and amplitude have more important meaning than the number of weave in the breathable waterproof fabrics. The puckering in breathable waterproof fabrics is mainly occurred by inherent and feeding pucker. The former if due to the insertion of sewing thread and the littler is caused by differential feeding when two pieces of fabric are fed into the gap between a press foot and needle plate.

• 한국환경성돌연변이발암원학회지
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• 제17권2호
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• pp.71-77
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• 1997

The single cell gel electrophoressis(SCGE) assay, also known as the comet assay, is a rapid, simple, visual and sensitive technique for measuring and analysing DNA breakage in mammalian cells. The SCGE or comet assay is a promising test for the detection of DNA damage and repair in individnal cells. It has widespread potential applications in DNA damage and repair studies, genotoxicity testing and biomonitoring. In this microgel electrophoresis technique, cells are embedded in agarose gel on microscope slides, iysed and electrophoresed under alkaline conditions. Cells with increased DNA damage display increased migration of DNA from the nucleus towards the anode. The length of DNA migration indicates the amount of DNA breakage in the cell. The comet assay is also capable of identifying apoptotic cells which contain highly fragmented DNA. Here we review the development of the SCGE assay, existing protocols for the detection and analysis of comets, the relevant underlying principles determining the behaviour of DNA and the potential applications of the technique.

• 한국공작기계학회:학술대회논문집
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• 한국공작기계학회 2002년도 춘계학술대회 논문집
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• pp.245-250
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• 2002

A successful on-line monitoring system for conventional machining operations has the potential to reduce cost, guarantee consistency of product quality, improve productivity and provide a safer environment for the operator. In fee-shape machining, typical signs of tool problems such as vibration, noise, chip flow characteristics and visual signs are almost unnoticeable without the use of special equipment. These characteristics increase the importance of automatic monitoring in fine-shape machining; however, sensing and interpretation of signals are more complex. In addition, the shafts of the micro-tools break before the typical extensive cutting edge of the tool gets damaged. In this study, the existence of a relationship between the characteristics of the cutting force and tool usage was investigated, and tool breakage detection algorithm was developed and the fellowing results are obtained. In data analysis, didn't use a relative error compare which mainly used in established experiment and investigated tool breakage detection algorithm in time domain which can detect AE and cutting force signals more effective and accurate.

• 한국생산제조학회지
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• 제9권5호
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• pp.144-149
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• 2000

The demands for robotic and automatic system are continually increasing in manufacturing fields. There have been many studies to monitor and predict the system, but they have mainly focused upon measuring cutting force, and current of motor spindle, and upon using acoustic sensor, etc. In this study, time series sequence of cutting force was acquired by taking advantage of piezoelectric type tool dynamometer. Radial cutting force was obtained from it and was available for useful observation data. The parameter was estimated using PAA(parameter adaptation algorithm) from observation data. ARMA(auto regressive moving average) model was selected for system model and second order was decided according to parameter estimation. Uncorrelation test was also carried out to verify convergence of parameter.

• Applied Microscopy
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• 제14권2호
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• pp.38-51
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• 1984

Fine structure of dormant and swollen conidiospore from Trichoderma koningii and the mechanism of protoplasting from the conidiospore were studied by scanning and transmission electron microscopy. The cell wall of dormant conidiospore was two-layered structure which consisted of electron dense outer layer and electron transparent inner layer. After 8.5 hrs incubation. the conidiospore was swollen and the outer layer of cell wall shown unequal thickness and partial breakage. Protoplast was released through the pore which has been formed by the breakage of outer layer and dissolution of newly synthesized cell wall for germ-tube formation. Swollen conidiospore and protoplast in releasing process contained various cell organelles and vacuoles with electron dense materials. The protoplast contained looser cytoplasm and had no cell wall materials outside of plasmamembrane.

• 한국정밀공학회지
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• 제21권1호
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• pp.133-139
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• 2004

High speed machining (HSM) can reduce machining time with the high metal removal rate by high speed spindle and feedrate. This paper supports HSM technology using the small size tool with the optimal tool path generation and modification of tool change. The optimum tool path is generated to reduce cutting length of cavity pattern and change the cutting tool for preventing the tool breakage by wear. The tool path is modified with the experiment data of tool wear and breakage to support tool change on reasonable time. The result can contribute to HSM technology of high hardness materials using the small size end-mill.