• Title/Summary/Keyword: Brassica crop

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SNP discovery and applications in Brassica napus

  • Hayward, Alice;Mason, Annaliese S.;Dalton-Morgan, Jessica;Zander, Manuel;Edwards, David;Batley, Jacqueline
    • Journal of Plant Biotechnology
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    • v.39 no.1
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    • pp.49-61
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    • 2012
  • This review summarises the biology, discovery and applications of single nucleotide polymorphisms in complex polyploid crop genomes, with a focus on the important oilseed crop $Brassica$ $napus$. $Brassica$ $napus$ is an allotetraploid species, and along with soybean and oil palm is one of the top three most important oilseed crops globally. Current efforts are well underway to $de$ $novo$ assemble the $B.$ $napus$ genome, following the release of the related $B.$ $rapa$ 'A' genome last year. The next generation of genome sequencing, SNP discovery and analysis pipelines, and the associated challenges for this work in $B.$ $napus$, will be addressed. The biological applications of SNP technology for both evolutionary and molecular geneticists as well as plant breeders and industry are far-reaching, and will be invaluable to our understanding and advancement of the $Brassica$ crop species.

Transcriptome Analysis in Brassica rapa under the Abiotic Stresses Using Brassica 24K Oligo Microarray

  • Lee, Sang-Choon;Lim, Myung-Ho;Kim, Jin A;Lee, Soo-In;Kim, Jung Sun;Jin, Mina;Kwon, Soo-Jin;Mun, Jeong-Hwan;Kim, Yeon-Ki;Kim, Hyun Uk;Hur, Yoonkang;Park, Beom-Seok
    • Molecules and Cells
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    • v.26 no.6
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    • pp.595-605
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    • 2008
  • Genome wide transcription analysis in response to stresses is essential to provide the basis of effective engineering strategies to improve stress tolerance in crop plants. In order to perform transcriptome analysis in Brassica rapa, we constructed a B. rapa oligo microarray, KBGP-24K, using sequence information from approximately 24,000 unigenes and analyzed cold ($4^{\circ}C$), salt (250 mM NaCl), and drought (air-dry) treated B. rapa plants. Among the B. rapa unigenes represented on the microarray, 417 (1.7%), 202 (0.8%), and 738 (3.1%) were identified as responsive genes that were differently expressed 5-fold or more at least once during a 48-h treatment with cold, salt, and drought, respectively. These results were confirmed by RT-PCR analysis. In the abiotic stress responsive genes identified, we found 56 transcription factor genes and 60 commonly responsive genes. It suggests that various transcriptional regulatory mechanisms and common signaling pathway are working together under the abiotic stresses in B. rapa. In conclusion, our new developed 24K oligo microarray will be a useful tool for transcriptome profiling and this work will provide valuable insight in the response to abiotic stress in B. rapa.

Production of Haploids from Proton Ion and Gamma-Ray Irradiation Treated $M_2$ Generation of Isolated Microspores in Brassica napus L. ssp. oleifera (앙성자 및 감마선을 처리한 유채 $M_2$ 세대의 소포자로부터 반수체 배발생)

  • Kim, Kwang-Soo;Li, Mei-Yang;Jang, Young-Seok;Park, Yoon-Jung;Bang, Jin-Ki
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.53 no.2
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    • pp.150-155
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    • 2008
  • This experiment was carried out to investigate the effect of proton ion and gamma-ray irradiation on microspore culture of the flower buds of $M_2$ generation in winter type of Brassica napus L. ssp. oleifera. The seeds of three rape varieties, 'Halla', 'Naehan' and 'Tammi' were pretreated with proton ion and gamma-ray 400 Gy and 600 Gy, respectively. When microspore culture techniques were used, embryogenesis was increased in some varieties by proton ion and gamma-ray irradiation treated flower buds of $M_2$ generation than control. In genotypes 'Naehan' showed the highest embryo production frequency, but 'Tammi' showed lowest embryo production frequency. Some of the embryoids developed directly into plantlets, whereas others developed abnormally multilobe. Plants were regenerated and successfully acclimatized in pots.

Breeding for Inprovement of Fatty Acid Composition in Rapeseed, Brassica napus L. Ⅶ. Studies on Classification of Spring Nature Grade of Double Zero Rapeseed Varieties (유채 지방산조성 개량육종에 관한 연구 제15보 양질유ㆍ양박 유채 육성계통들의 춘파성 정도 분류)

  • Lee, J.I.;Kwon, B.S.;Kim, S.K.;Bang, J.K.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.28 no.2
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    • pp.233-239
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    • 1983
  • In order to obtain basic date for the cultivation and the breeding of double zero varieties of rapeseed, spring nature grade were investigated. Spring nature grade could be classified to 8 classes by the method of material maturity. The double zero varieties, Ⅶ group of spring adaptability has been developed from the cross "Oro" and "Midas" classified as summer type. A significant negative correlation was found between the spring adaptability and seed yield. On the other hand, a highly significant negative correlation was found between maturation and spring adaptability, cold resistnce and spring adaptability, plant from and spring adaptability. Ⅵ and Ⅶ group close to summer type showed spring growing adaptability, and 0, I and II group were appeared as winter type, autumn growing adaptability with cold tolerance.with cold tolerance.

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The Cross Ability and the Phenotypic Characteristics of F1 Hybrid in the Interspecific Crosses between Brassica napus and B. campestris, B. rapa (Brassica 속 식물 내 종간교잡에 따른 교잡효율 및 F1잡종의 표현형질)

  • Kim, Kwang-Soo;Lee, Yong-Hwa;Jang, Young-Seok;Choi, In-Hu
    • Korean Journal of Plant Resources
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    • v.28 no.1
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    • pp.119-125
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    • 2015
  • Interspecific crosses between rapeseed (Brassica napus L.) and chinese cabbage (B. campestris var. pekinensis Makino) and turnip (B. rapa L.) were made in order to examine the cross possibility and morphological phenotype of $F_1$ hybrid. The growth of pollen tube of cross between rapeseed and chinese cabbage was more rapid than cross between rapeseed and turnip. Silique formation and seed setting in silique were relatively high in the cross between rapeseed and chinese cabbage. The percentage of silique set from interspecific cross between rapeseed and chinese cabbage was 90.6% and higher 23.3% than the percentage of silique from interspecific cross rapeseed and turnip. The average number of seed per silique obtained from the cross rapeseed and chinese cabbage, and rapeseed and turnip reached 15.5 and 11.6, respectively. The morphological phenotypes of $F_1$ hybrid plants obtained from seeds in the cross between rapeseed and chinese cabbage resembled rapeseed mainly, but leaf length and leaf width were increased. The size, shape and lobation of leaves of $F_1$ hybrid plants from interspecific cross between rapeseed and turnip were intermediated between their parent species, but color of leaves was dark-green.