• Title/Summary/Keyword: Brassica campestris ssp. pekinensis

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Sodium Hypochlorite Solution As a Chemical Wounding Agent for Improving Agrobacterium-mediated Chinese Cabbage Seed Transformation (Sodium hypochlorite처리에 따른 배추종자의 Agrobacterium이용 형질전환 증대)

  • Shin Dong-Il;Park Hee-Sung
    • Journal of Life Science
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    • v.15 no.6 s.73
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    • pp.1034-1036
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    • 2005
  • Chinese cabbage (Brassica campestris ssp. napus var. pekinensis Makino) seeds/seedlings were transformed via vacuum-infiltration with recombinant Agrobacterium tumefaciens LBA4404 cells. The agroinfiltration method was determined to be unsuccessful for Chinese cabbage transformation during the analysis of hepatitis B surface antigen expression by ELISA. However, treatment of sodium hypochlorite solution, prior to agroinfiltration, to pregerminated or germinating 1 day- or 2 days-old seeds was proven effectively to enhance transformation efficiency, suggesting that chemical wounding caused by sodium hypochlorite reaction might facilitate Agrobacterium infection and, therefore, transient gene expression in Chinese cabbage sprouts.

Effect on Yield of Cabbage and Soil Chemical Properties with Nitrogen and Potash (질소(窒素) 및 가리(加里)의 시용(施用)이 배추의 수량(收量)과 토양화학적(土壤化學的) 성질(性質)에 미치는 영향(影響))

  • Oh, Wang-Keun;Kim, Seoung-Bae;Kang, An-Seok
    • Korean Journal of Soil Science and Fertilizer
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    • v.17 no.3
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    • pp.253-257
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    • 1984
  • A field experiment was conducted in order to secure the basic information on the rational application of nitrogen and potassium for autumn growing Chinese cabbage (Brassica campastris ssp. pekinensis, var.; Miho 70 days). The results of the experiment are summarized as follows: 1. Potassium effect was observed where nitrogen applied either less than 15kg N/10a and heavy dose of 25kg N/10a. No potassium effect was observed where 20kg N/10a applied. In the case of 25kg N/10a, potassium effect was observed only in total weight but failed yield marketable product weighing over 1 kilogram per cabbage. 2. Nitrogen application, in general, tends to lower the soil pH and it is particularly true when heavy dose of over 20kg N/10a is applied. As a result, Chinese cabbage has increasingly removed soil born potassium and reduced exchangeable potassium content of the soil. 3. Oven dried cabbage which received 25kg N/10a plus potassium showed a low concentration of calcium and this phenomena seems to be attributable to the acidification of soils and it further caused failure in producing cabbages of marketing value.

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Effects of Vernalization, Temperature, and Soil Drying Periods on the Growth and Yield of Chinese Cabbage (춘화, 온도와 토양건조 기간에 따른 배추의 생장 및 수량)

  • Lee, Sang Gyu;Lee, Hee Ju;Kim, Sung Kyeom;Choi, Chang Sun;Park, Sung Tae;Jang, Yoon Ah;Do, Kyung Ran
    • Horticultural Science & Technology
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    • v.33 no.6
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    • pp.820-828
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    • 2015
  • This study was carried out to determine the effects of vernalization, temperature, and soil water deficit (SD) on mesophyll cells, growth, and yield of Chinese cabbage (Brassica campestris L). The palisade parenchyma and spongy tissues of Chinese cabbage were observed under full irrigation and two weeks of SD treatment. These cells were severely collapsed by four weeks SD treatment. The SD treatment had the greatest influence on the growth of Chinese cabbage among the tested treatment factors (vernalization, temperature, and SD), growth significantly decreased by severe drought treatment (four weeks SD treatment). In addition, the relative growth rate, unit leaf rate, leaf area ratio, specific leaf area, and leaf weight ratio were significantly affected by SD treatment; however, other individual factors and their combined treatments did not influence the analyzed growth parameters. The yield under vernalization after high temperature and full irrigation treatments was 3,056 kg/10 a, which was the greatest among all the tested treatments, while four-week SD treatment significantly reduced the yield. Head formation of Chinese cabbage was not altered under SD treatment, and vernalization treatments did not induce bolting. Our results indicated that collapsing mesophyll cells and reduced growth and yield were induced by SD treatment. Thus we suggest that optimal irrigation system should be install to avoid or overcome crippling drought conditions in the open field.

Promotive Effects of Geraniol on Radicle Growth of Several Vegetables and Leaf Growth of Brassica campestris (Geraniol의 채소 작물 유근생장 및 배추 생장 촉진효과)

  • Choi, Geun-Hyoung;Jeong, Dong-Kyu;Park, Byung-Jun;Cho, Nam-Jun;Hong, Jin-Hwan;Kim, Jin-Hyo
    • The Korean Journal of Pesticide Science
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    • v.19 no.4
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    • pp.399-401
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    • 2015
  • This study investigated the plant growth promoting activity of geraniol on Chinese cabbage (Brasscia campestris ssp. Pekinensis Rupr.), Pak Choi (Brassica rapa L.), Crown daisy (Chrysanthemum coronarium L.), Iceberg lettuce (Lactuca sativa L.) and Leaf perilla (Perilla frutescens Britt.). Geraniol at 0.5 to $10mg\;L^{-1}$ promoted radical growth of Chinese cabbage Pak Choi, Crown daisy, Iceberg lettuce and leaf perilla by >30%. In the pot experiments, geraniol treatment at $20mg\;L^{-1}$ by drenching increased up to 190% of the aerial part growth promotion. By comparison, foliar application showed 124% promoting activity only at $100mg\;L^{-1}$.

Inhibition Effects Against Plant Pathogenic Fungi and Plant Growth Promotion by Beneficial Microorganisms (유용 미생물을 활용한 식물 병원 곰팡이의 억제와 식물 생장촉진 효과)

  • Jung, Jin Hee;Kim, Sang Woo;Kim, Yun Seok;Lamsal, Kabir;Lee, Youn Su
    • The Korean Journal of Mycology
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    • v.41 no.2
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    • pp.118-126
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    • 2013
  • The experiment was carried out to analyze the inhibition effect of plant pathogenic fungi and growth promotion activity induced by the bacterial strains isolated from peatmoss. Among the isolated bacterial strains, B10-2, B10-4, B10-5 and B10-6 which showed more than 30% inhibition rate against Botrytis cinerea and Rhizoctonia solani in vitro, were further analyzed in the greenhouse for the growth promotion activity on lettuce (Lactuca sativa), pak-choi (Brassica compestris L. ssp. chinensis) and Chinese cabbage (Brassica campestris L. ssp. pekinensis). The results showed the treatment of B10-4 on lettuce showed the highest growth promotion activity with the leaf area ($169.17cm^2$), fresh weight (leaf: 40.29 g, root: 8.80 g)and dry weight (leaf: 11.24 g, root: 4.17 g), which was about two folds as compared to control. On pak-choi, the growth promotion rate was the highest with the leaf area of $112.87cm^2$, leaf fresh weight of 60.70 g, root fresh weight of 3.37 g, leaf dry weight of 14.34 g, and root dry weight of 1.90 g. As a result of treatment of B10-13 on chinese cabbage, the growth promotion rate was the highest with the leaf area ($293.56cm^2$), fresh weight (leaf: 113.67 g, root: 2.40 g) and dry weight (leaf: 6.03 g, root: 0.53 g). The production of Indole Acetic Acid (IAA) and Indole-3-Butylic Acid (IBA) were also analyzed in these bacterial isolates. The IAA and IBA analyses were carried out in all bacterial isolates each day within the 5 days of incubation period. The highest production of IAA was observed with $112.57{\mu}g/mg$ protein in B10-4 after 3 days of incubation and IBA production was the highest in B10-2 with $58.71{\mu}g/mg$ protein after 2 days of incubation. Also, phosphate solubilizing activity was expressed significantly in B10-13 in comparison to that of other bacterial isolates. Bacterial identification showed that B10-2 was Bacillaceae bacterium and B10-5 was Bacillus cereus, B10-4 and B10-6 were Bacillus sp. and B-13 was Staphylococcus sp. by ITS sequence.

Effect of the Organic Fertilizer Mixed with Various Recycled Coir Substrates on Chinese Cabbage(Brassica Campestris Ssp. Pekinensis) and Lettuce(Lactuca Sativa) (폐코이어를 재활용한 혼합 유기질 비료가 배추와 상추의 생육에 미치는 영향)

  • Lee, Gyu-Bin;Park, Eun-Ji;Park, Young-Hoon;Choi, Young-Whan;Suh, Jeong-Min;Kang, Jum-Soon
    • Journal of Environmental Science International
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    • v.24 no.9
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    • pp.1221-1231
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    • 2015
  • The present study was conducted to find a way to recycle the coir substrate by investigating changes in its physical and chemical properties based on the number of use year. Specific gravity of unused coir substrate was $0.212g/cm^3$, while it was higher for the substrate used for 2 years. Porosity was different depending on the number of use year. The porosity of unused substrate was 51.9%, but it increased to 68.6% after used for 2 years. In general, physical and chemical properties were better in the coir substrate used for 2 years than in unused one. The number of leaves, leaf area, flesh weight and dry weight of oriental cabbage and lettuce were higher in coir substrate used for 2 years than those in unused one. Whereas, no significant difference was observed between the substrates used for one year and 2 years, indicating that the one time-used wast substrate could be recycled for cultivating vegetables. Growth of the vegetables was improved when organic fertilizer composed of complex organics with different mixing ratios was provided to the coir substrate, compared to untreated plot. The optimum mixing ratio of the wast substrate and complex organics was 2:8(v/v) for fertilization using wast coir substrate. Therefore, coir substrate generally wasted after being used for one time was reuseable by supplying organic fertilizer.

Effect of Horticultural Media with Recycled Coir Substrates on Growth of Chinese Cabbage and Lettuce Crop (코이어 배지를 재활용한 혼합 상토가 배추 및 상추의 생육에 미치는 영향)

  • Lee, Gyu-Bin;Choe, Yun-Ui;Park, Eun-Ji;Park, Young-Hoon;Choi, Young-Whan;Son, Beung-Gu;Kang, Jum-Soon
    • Journal of Environmental Science International
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    • v.27 no.11
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    • pp.937-946
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    • 2018
  • This study investigated the applicability of horticultural media with recycled coir substrates the growth of Chinese cabbage (Brassica campestris L. ssp. Pekinensis) and lettuce (Lactuca sativa L.) crop. The six different types of coir based substrates were A, Coir 45: Perlite 35: Vermiculite 12: Zeolite 8 (%), B, Coir 55: Perlite 25: Vermiculite 12: Zeolite 8 (%), C, Coir 65: Perlite 15: Vermiculite 12: Zeolite 8 (%), D, Coir 75: Perlite 5: Vermiculite 12: Zeolite 8 (%), E, Coir 85: Perlite 5: Vermiculite 5: Zeolite 5 (%) and F, nursery media (control). The pH and Electric conductivity of the horticultural nursery media were 6.06-7.00 and $0.45-1.10dS/m^{-1}$, respectively. The nursery media containing coir substrates had higher level of Total N, Ca, K, Mg and P than those without coir. Additionally, it was observed that the growth of Chinese cabbage was the best on D (containing 75% coir) while that of lettuce was the best on E (containing 85% coir). In general, when substrates containg a higher percentage of coir were used, the growth of Chinese cabbage and lettuce was ideal. Additionally, the P, Ca, and Mg content in both plants was not significantly altered by the amount of coir present in the media. However, with an increase in the amount of coir substrate, the chlorophyll, N, and K content was increased. After harvesting, there was no significant difference in the chemical properties of the horticultural nursery media of both plants. Thus, it can be suggested that, coir substrate after a single use could be recycled as horticulture nursery media.

Changes in Myrosinase Activity and Total Glucosinolate Levels in Korean Chinese Cabbages by Salting Conditions (배추 절임조건에 따른 Myrosinase 활성 및 Total Glucosinolates 함량 변화)

  • Hwang, Eun-Sun
    • Korean journal of food and cookery science
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    • v.26 no.1
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    • pp.104-109
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    • 2010
  • Korean Chinese cabbage (Brassica campestris L. ssp. pekinensis) is one of the major cruciferous vegetables. Cruciferous vegetables contain a series of relatively unique secondary metabolites of amino acids called glucosinolates. Although glucosinolates do not appear to be bioactive, they are hydrolyzed by plant myrosinase when the cells in plants are damaged, and release biologically active compounds such as isothiocyanates, nitriles, and thiocyanates. The objective of this study was to determine the myrosinase activity and total glucosinolate levels of Korean Chinese cabbages by different salting times (0, 12, 18, and 24 h) and salt concentrations (6, 10, 14%). The total water content, salt content, and pH of brined cabbages decreased with increasing salting time. The myrosinase activity as determined by a glucose kit, decreased with increasing salting time and salt content. The total glucosinolates were purified using an anion exchange column and measured by UV-visible spectrophotometer. The fresh Korean Chinese cabbages contained $25.38{\pm}1.45\;{\mu}mol/g$ dry weight of glucosinolates. However, the total glucosinolates of brined cabbages decreased with increasing salting time and salt concentration. After 24 h of salting time, the total glucosinolates of brined cabbages rapidly decreased by $16.12{\pm}11.09$, $11.25{\pm}10.91$, $9.29{\pm}10.73\;{\mu}mol/g$ in 6%, 10%, and 14% salt solution, respectively. Overall, the total glucosinolate levels of Korean Chinese cabbages were found to vary inversely with salting time and salt concentration.

A Thermal Time - Based Phenology Estimation in Kimchi Cabbage (Brassica campestris L. ssp. pekinensis) (온도시간 기반의 배추 생육단계 추정)

  • Kim, Jin-Hee;Yun, Jin I.
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.17 no.4
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    • pp.333-339
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    • 2015
  • A thermal time-based phenology model of Kimchi cabbage was developed by using the field observed growth and temperature data for the purpose of accurately predicting heading and harvest dates among diverse cropping systems. In this model the lifecycle of Kimchi cabbage was separated into the growth stage and the heading stage, while the growth amount of each stage was calculated by optimal mathematical functions describing the response curves for different temperature regimes. The parameter for individual functions were derived from the 2012-2014 crop status report collected from seven farms with different cropping systems located in major Kimchi cabbage production area of South Korea (i.e., alpine Gangwon Province for the summer cultivation and coastal plains in Jeonnam Province for the autumn cultivation). For the model validation, we used an independent data set consisting of local temperature data restored by a geospatial correction scheme and observed harvest dates from 17 farms. The results showed that the root mean square error averaged across the location and time period (2012-2014) was 5.3 days for the harvest date. This model is expected to enhance the utilization of the Korea Meteorological Administration's daily temperature data in issuing agrometeorological forecasts for developmental stages of Kimchi cabbage grown widely in South Korea.

Growth Characteristics of Herbaceous Plants by Soil Condition to Revitalize the Urban Agriculture (도시농업 활성화를 위한 토양조건별 초본식물의 생육특성)

  • Park, Won Jei;Han, Kyung Hwan;Kwon, Soon Hyo;Park, Mi Ok;Koo, Bon Hak
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.15 no.2
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    • pp.1-8
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    • 2012
  • This study is carried out to find the most optimal soil-plant combination in the urban agriculture by analyzing the association of soil base material which is being used in the urban agriculture with the growth of plants. 4 types of easily purchased soil (bed soil(A), animal vermicast soil(B), earth worm soil(C) and matured compost(D)) verified in aspects of effect and safety of soil in terms of growth of crop is selected as experimental soil and B, C, D type soils are mixed with granite soil at the ratio of 7 : 3. And granite soil(E) is set as a controlled soil and is compared to verify the effect of the experimental mixed soil. Herbaceous plants are classified into the fruit vegetables (Lycopersicon esculentum Mill. and Capsicum annuum L.), leafy vegetables (Brassica campestris L. ssp. Pekinensis and Lactuca sativa L.), medicinal vegetables (Chrysanthemum zawadskii var. latilobum and Liriope platyphylla F. T. Wang & T. Tang). The results of comparison of growth of herbaceous plants in different soil types showed that fruit vegetables and leafy vegetables in general had excellent growth in D type soil mixture and A type soil in general and had the poorest growth in E type (controlled) soil. 'Chrysanthemum zawadskii var. latilobum' had the excellent growth in D type, B type, C type mixed soil and A type soil and E type (controlled) soil are followed in order. In the case of 'Liriope platyphylla F. T. Wang & T. Tang', the difference in growth by each soil was shown to be insignificant. Therefore, the soil applied in the urban agriculture varies depending on each species of herbaceous plants, but it is considered effective to cultivate herbaceous plant which is economical and productive by using D type mixed soil which can be recycled and inexpensive compared with other experimental soils in the urban agriculture.