• The Journal of the Korean bone and joint tumor society
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• v.15 no.2
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• pp.111-121
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• 2009

Background: Osteosarcoma is one of the most common primary malignant tumors of bone occurring mainly in children and adolescents. Although surgery combined with chemotherapy has markedly improved patient survival during the last years, the use of anticancer drugs is still associated with serious problem, such as the frequent acquisition of drug-resistant phenotypes and occurrence of "secondary malignancies". Several solid tumors display enhanced expression of matrix metalloproteinases (MMPs), and recently clinical trials have been initiated on MMP-inhibitors. On the other hand, bisphosphonates (BPs) are inhibitors of bone resorption, and widely used to treat osteoclast-mediated bone diseases. Also they appear to possess direct antitumor activity. Methods: One osteosarcoma cell line (U2OS) was treated with ibandronate (0, 0.1, 1, $10{\mu}M$) for 48 hours. Cell viabilities were determined using MTT assay, the mRNA levels of MMP-2 and MT1-MMP were detected by reverse-transcription polymerase chain reaction, the amount of MMP-2 and MT1-MMP protein were measured by Westernblot, the activities of MMP-2 were observed by Gelatin zymography, and Matrigel invasion assays were used to investigate the invasive potential of osteosarcoma cell lines before and after ibandronate treatment. Results: The invasiveness of U2OS cell line was reduced dose-dependently following 48 hour treatment of up to $10{\mu}M$ of the ibandronate at which concentration no cytotoxicity occurred. Furthermore, the gelatinolytic activities and protein and mRNA levels of MMP-2 and MT1-MMP were also suppressed by increasing ibandronate concentrations. Conclusion: Given that MMP-2 is instrumental in tumor cell invasion, it is very likely that the reduction in osteosarcoma cell invasion by ibandronate is a consequence, at least in part, of suppressed expression of both MMP-2 and MT1-MMP. Isolation of a molecule (s) responsible for the bisphosphonate inhibition of tumor cell invasion would pave the way for the development of a new generation of metastasis inhibitors.

• Korean Journal of Clinical Laboratory Science
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• v.39 no.2
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• pp.113-121
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• 2007

Hearing loss is a common congenital disorder that is frequently associated with mutations in the Cx26 gene (GJB2). Recently, the mutation analysis of GJB2 has been used in a newborn screening test for the detection of hearing impairment. Population-based studies should be performed before the application of genetic testing for the identification of deaf newborns. In this study, 8 positions of GJB2 mutations-including 35delG, 167delT, 235delC, V27I, V37I, M34T, E114G, and I203T-were analyzed using PCR-direct sequencing in a total of 437 healthy Korean neonates. DNAs from dried blood spots were extracted using a commercial DNA extraction kit. The PCR-amplified products (783 bps) of the GJB2 gene were detected using 2% agarose gel electrophoresis and subjected to direct sequencing. The sequences were compared with those in the GenBank database by using the BLAST program. In this study, 5 GJB2 mutations -including V27I (79G>A), V37I (109G>A), E114G (341A>G), I203T (608T>C), and 235delC- were found. Of the 437 neonate samples, 301 subjects showed GJB2 mutations (68.9%, 301/437). The V27I mutation was found in 271 subjects and was the most frequent (62.0%, 271/437). The E114G, I203T and V37I mutations were shown in 146, 17 and 14 subjects, respectively. The 235delC mutation was found in 1 subject. The E114G mutation was frequently accompanied by the V27I mutation. V27I/E114G (97.2%, 143/147) was the most common double mutation and 3 subjects had the double mutation V27I/I203T. A triple mutation, V27I/E114G/I203T, was found in 1 subject. In conclusion, PCR-direct sequencing is a convenient tool for the rapid detection of GJB2 mutations and this data might provide information for the genetic counseling of the GJB2 gene.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.12
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• pp.1701-1707
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• 2005

This study deals with the characterization of porcine PIK3C3 and association tests with quantitative traits. PIK3C3 belongs to the class 3 PI3Ks that participate in the regulation of hepatic glucose output, glycogen synthase, and antilipolysis in typical insulin target cells such as those in the such as liver, muscle system, and fat. On the analysis of full-length mRNA sequence, the length of the PIK3C3 CDS was recorded as 2,664 bps. As well, nucleotide and amino acid identities between human and pig subjects were 92% and 99%, respectively. Five SNPs were detected over 5 exons. We performed genotyping by using a SNP C2604T on exon24 for 145 F$_2$ animals (from a cross between Korean native boars and Landrace sows) by PCR-RFLP analysis with Hpy8I used to investigate the relationship between growth and fat depot traits. In the total association analysis, which doesn' consider transmission disequilibrium, the SNP showed a significant effect (p<0.05) on body weight and carcass fat at 30 weeks of age as well as a highly significant effect (p<0.01) on back fat. In an additional sib-pair analysis, C allele still showed positive and significant effects (p<0.05) on back fat thickness and carcass fat. Moreover, the effects of C allele on the means of within-family components for carcass fat and back fat were estimated as 2.76 kg and 5.07 mm, respectively. As a result, the SNP of porcine PIK3C3 discovered in this study could be utilized as a possible genetic marker for the selection of pigs that possess low levels of back fat and carcass fat at the slaughter weight.

• 제어로봇시스템학회:학술대회논문집
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• 2004.08a
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• pp.138-142
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• 2004

In this paper, we present a new method for monitoring of ECU's sensor signals of vehicle. In order to measure the ECU's sensor signals, the interfaced circuit is designed to communicate ECU and the Embedded Linux is used to monitor communication result through Web the Embedded Linux system and this system is said "ECU Interface Part". In ECU Interface Part the interface circuit is designed to match voltage level between ECU and SA-1110 micro controller and interface circuit to communicate ECU according to the ISO, SAE communication protocol standard. Because Embedded Linux does not allow to access hardware directly in application level, anyone who wants to modify any low level hardware must develop device driver. To monitor ECU's sensor signals the most important thing is to match serial level between ECU and ECU Interface Part. It means to communicate correctly between two hardware we need to match voltage and signal level, and need to match baudrate. The voltage of SA-1110 is 0 ${\sim}$ +3.3V and ECU is 0 ${\sim}$ +12V and, ECU's communication Line K does multiple operation so, the interface circuit is used to match voltage and signal level. In Addition to ECU's baudrate is 10400bps, it's not standard baudrate in computer environment. So, we need to develop a device driver to control the interface circuit, and change baudrate. To monitor ECU's sensor signals through web there's a network socket program is working in Embedded Linux. It works as server program and manages user's connections and commands. Anyone who wants to monitor ECU's sensor signals he just only connect to Embedded Linux system with web browser then, Embedded Linux webserver will return the ActiveX webbased measurement software. It works in web browser and inits ECU, as a result it returns sensor signals through web. All the programs are developed with GCC(GNU C Compiler) and, webbased measurement software is developed with Borland C++ Builder.

• Archives of design research
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• v.19 no.1 s.63
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• pp.141-150
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• 2006

The animation should be produced to attract the audience's interests. Its characters will have the intended personalities through the interactions with the audiences, discarding the attributes of the nature. These personalities form the brand identity when they are exposed visually and will be powerful brand assets which lead the animation industry to the high-value added products. The brand identity of the character, the brand assets, can be used for various products in form of licensing and is noted to make an affirmative leverage effect. In this regard, the author has conducted an empirical research on the animation characters from the viewpoint of the brand, adopting, in particular, the Brand Personality Scale (BPS), which is the output of J. Aaker's (1997) study on brand personality defined as the human properties in relation with the brand. In addition, this study determines the correlation among the animation, brand and consumers based on the Sirgy's study (1982) resulting in that the better the brand and the consumer's personality are matched, the more the brand attitude is improved. In consequence, it is found that the animation characters have three personality levels such as refinement/ability, integrity and interests. The consumer's personality is divided into the 'practical ego-image' and the 'ideal ego-image' in the survey, and the survey result shows that the brand personality of the animation character exists between them.

• BMB Reports
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• v.48 no.3
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• pp.153-158
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• 2015

As FK506 binding proteins (FK506BPs) are known to play an important role in the regulation of a variety of biological processes related to cell survival, this study was designed to examined the protective effects of FK506 binding protein 12 (FK506BP) on low humidity air flow induced dry eye in a rat model using transduced PEP-1-FK506BP. After the topical application of PEP-1-FK506BP, tear volumes were markedly increased and significant prevention of cornea damage was observed compared with dry eye rats. Further, immunohistochemical analysis demonstrated that PEP-1-FK506BP markedly prevented damage to the cornea, the bulbar conjunctiva, and the palpebral conjunctiva epithelial lining compared with dry eye rats. In addition, caspase-3 and PARP expression levels were found to be decreased. These results demonstrated that topical application of PEP-1-FK506BP significantly ameliorates dry eye injury in an animal model. Thus, we suggest that PEP-1-FK506BP can be developed as a new ophthalmic drop to treat dry eye diseases.

• Journal of Life Science
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• v.20 no.12
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• pp.1859-1866
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• 2010

It is reported that about 80% of deer antlers (Cervi Pantotricuhum Cornu) produced in the world are consumed in Korea. Fraudulent replacement or mislabeling of costly deer antlers with cheaper ones, however, is one of the most common problems in the Korean deer antler market. Therefore, there is a continuous need for the development of genetic markers to discriminate between genuine and fraudulent deer antlers. This study was performed to develop a method for the identification and authentication of deer antlers using nucleotide sequence analysis against displacement loop of mitochondrial genome among four deer antlers, Cervus eleaphus sibericus, Cervus eleaphus bactrianus, Cervus eleaphus Canadensis, and Cervus eleaphus, originated from Russia, China, North America and New Zealand, respectively. As a result, multiple-alignment of mitochondrial displacement (D) loop region in 1.2 kb showed that, among the four deer antlers, a deleted sequence of about 70 bps was only found in Cervus elaphus bactrianus from China. Finally, Cervus elaphus bactrianus among nine samples of deer antlers were successfully identified by PCR using primer amplifying deleted D-loop. Cervus elaphus bactrianus was also confirmed from cloning the PCR products and their nucleotide sequence analyses were confirmed. However, no marker to identify Cervus eleaphus sibericus, Cervus eleaphus canadensis and Cervus eleaphus were found in the nucleotide sequences of mitochondrial D-loop. Our results suggest that PCR for deleted D-loop region of mitochondrial DNA are useful for identification and authentication of deer antlers of Cervus elaphus bactrianus originating from China.

• The Transactions of the Korea Information Processing Society
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• v.4 no.1
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• pp.215-223
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• 1997

The advantages of service in satellite links are representatively a long distance and large capacity transfer and simultaneous communication. And terrestrial links are of high speed and high quality data transfer Therefore a combined form of characteristics of satellite and terrestrial links is desirable to furnish a good quality service to subscribers. And in satellite links an analysis method and a simulation method of file transfer are conducted to evaluate the efficiency of file transfer. The parameters are used the message length and transmission time, and the number of chain record and transmission time. From the results of evaluation, in satellite links, it is found that when the message length is shorter than 512 bytes, the file transmission time becomes extremely long and when the message length is set at 1024 bytes, the file transmission time abruptly increase from 2048 bytes. Also it is confirmed that when the number of chain record is under 15, the file transmission time becomes extremely long and if the number increase up to 90,the time becomes gradually short and over 90,it becomes constant.Therefore,from the result of file transmission in th case of utilizing satelite links,it is confirmed that the optimum message length in the minmum transmission time is present closely at 1024 bytes.Also it is confirmed that when the satelite transmission sped becoms smaller from 24kbps to2400bps,the optimum message length also becomes smaller from 640 bytes to 256bytes.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.4
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• pp.1342-1347
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• 2010

Various researches have been studied on WSN(wireless sensor network) for barbed wire entanglements surveillance applications such as industry facilities, security area, prison, military area, airport, etc. Currently, barbed wire entanglements surveillance is formed wire sensor network environment. Traditional wire sensor network guarantee high data transmission rate. Therefore, wire sensor network use fast fourier transform of data of high transmission rate for extraction of feature parameter. However, wireless sensor network in comparison with wire sensor network has very low data transmission rate. Therefore, wireless sensor network doesn't use fast fourier transform of wire sensor network for extraction of feature parameter. In this paper, proposed method use 1 level approximation coefficient of DTW(dynamic time-warped) algorithms based on DWT(discrete wavelet transform) for extraction of detection feature parameter and classification feature parameter for barbed wire entanglements surveillance. l level approximation coefficient have time information and frequency information of signal. Therefore, Dynamic time-warped algorithms based on discrete wavelet transform improve detection and classification of target rather than using energy of signal.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.41 no.1
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• pp.23-34
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• 2004

This paper presents architecture and control method of packet scheduler to guarantee QoS of high quality streaming services in high-speed packet-switched networks. Since streaming services need far more stringent QoS requirements than the typical sort of burst data applications, they should be guaranteed minimum bandwidth and end-to-end delay bound to each flow, regardless of the behavior of other flows. To meet these requirements, a packet scheduler isolate a flow from the undesirable effects of other flows and provides end-to-end delay guarantees for individual flow and divides stringently the available link bandwidth among flows sharing the link. Until now, many vendors are developing traffic management chips running at 10Gbps, but most of chips have drawbacks to support high quality streaming services. In this paper, we investigate the drawbacks of commercial TM chips and traffic characteristic of streaming services and present implementation frameworks of the proposed packet scheduler. Finally, we analyze the simulation results of the proposed scheduler.