• 한국식품과학회지
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• 제25권5호
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• pp.461-467
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• 1993

마이크로파 가열방법에 따른 감자친분의 특성을 검토하기 위하여 마이크로파를 직접 가열한 전분(A군)과 감자에 마이크로파를 가열하여 분리한 전분(B군)을 대상으로 이화학적 특성을 비교분석한 결과는 다음과 같다. 감자전분의 단백질과 회분 함량은 마이크로파 가열에 의하여 거의 변화되지 않았으나, 유리지질은 마이크로파 가열시간이 길수록 감소한 반면, 결합지질은 마이크로파 가열시간이 길수록 증가하였다. 인의 함량은 마이크로파 가열시간이 길수록 감소하였으며 전분에 마이크로파를 직접 가열한 A군 보다 감자에 마이크로파를 가열하여 분리한 전분인 B군의 변화 정도가 컸다. 광학 및 편광현미경으로 관찰한 전분입자의 형태와 복굴절현상은 마이크로파 가열방법, 가열시간에 따른 차이는 나타나지 않았으나, SEM으로 관찰한 입자의 표면형태는 A군의 경우 300초 가열시 약간의 변형이 나타났고, B군은 180초 때부터 표면의 변화가 나타났으나 변화정도는 A군보다 더 적었다. 물결합능력은 마이로파 가열시간이 길수록 A군, B군 모두 증가되었고, 아밀로오스 함량 및 Blue value는 마이크로파 가열 시간이 길수록 A군, B군 각각 감소하였다. 팽윤력과 용해도는 마이크로파 가열시간이 길어짐에 따라 각각 감소하였는데 마이크로파 가열방법에 따라 다소 차이가 있었다. 감자전분의 X-선 회절도는 A군의 경우 회절각도$(2{\theta})$ $5.4^{\circ}$에서의 peak 높이가 감소되었으나, B군은 회절선의 변화가 없었고 전체적인 결정성이 약간 증가하였다.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1998년도 Proceedings of UNESCO-internetwork Cooperative Regional Seminar and Workshop on Bioassay Guided Isolation of Bioactive Substances from Natural Products and Microbial Products
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• pp.185-185
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• 1998

As part of our continuing attempts to evaluate biologically active compounds from fruiting bodies of cultivated fungus of Paecilomyces japonicus Yasuda, we conducted series of experiments on various fractions and compounds isolated by systematic fractionations. Our main efforts were concentrated on searching for compounds showing antitumor activities, which were tested on mice carrying Sarcoma-180 ascitic tumor. The antitumor activity was assessed by the life spans after these mice were administered Lp. with test compounds for consecutive 20 days. One of two pure compounds, which we have isolated to date, demonstrated significant prolongation of life span. ( Mean Survival Time: 30.3 days compared to that of control: 23.6 days). Structural analysis showed that this compound corresponds to D-mannitol. On the other hand, Ergosterol, another isolated pure compound didn't show efficient antitumor activity. We also obtained water-soluble fractions containing protein-bound polysaccharides and n-butantol fractions, which showed strong antitumor activities, 35.4(150%) and 32.1(136.0%) days of MST, respectively. In SRB assay, however, the test materials didn't show any toxic effects, but the level of acid phosphatase increased significantly when they were applied in cultured macrophage in vitro. Therefore, we concluded that antitumour activities might be attributed to immunostimulating rather than cytotoxic effects. Further experiments are underway to purify and structurally characterize new antitumour compounds from the active fractions.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1995년도 춘계학술대회
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• pp.81-81
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• 1995

It is well known that Src Homology 2(SH2) domain in many intracellular signal transduction proteins is very important. The domain has about 100 amino acid residues and bind phosphotyrosine-containing peptide with high affinity and specificity. Lck SH2 domain is a Src-like, lymphocyte-specific tyrosine kinase. An 11-residue phosphopeptide derived from the hamster polvoma middle-T antigen, EPQp YEEIPIYL, binds with an 1 nM dissociation constant to Lck SH2 domain. And it is known that the phosphotyrosine and isoleucine residues of the peptide are tightly bound by two well-defined pockets on Lck SH2 domain's surface. To investigate the conformational changes during complexation of SH2 domain with phosphopeptide we have performed the molecular dynamics simulation for Lck SH2 domain with peptide and peptide-free form at look in aqueous solution. More than 3000 water molecules were incorporated to solvate Lck SH2 domain and peptide. Periodic boundary condition has been applied in molecular dynamics simulation. Data analysis with the results of that simulation shows that the phosphopeptide makes primary interaction with the Lck SH2 domain at six central residues, The comparison of the complexed and uncomplexed SH2 domain structures in solution has revealed only relatively small change. But the hydrophilic and hydrophobic pockets in the protein surface show the conformational changes in spite of the small structural difference between the complex and peptide-free forms.

• 한국식품과학회지
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• 제27권5호
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• pp.658-665
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• 1995

Pectinestrase(PE) 중에 열에 안정한 thermostable pectineterase(TSPE)를 열에 불안정한 thermolabile pectinesterase(TLPE)로부터 구분하기 위하여 $70^{\circ}C$에서 $5{\sim}10$분간 가열 처리하였다. TSPE의 추출 수율을 증가시키기 위하여 추출시간, pH, 온도, NaCl의 농도 그리고 세포벽을 분해하는 가수분해 효소의 사용 등 여러가지 변화를 주어 개발한 최적 조건은 오렌지 착즙액의 pH 4.14와 같은 1 M NaCl의 acetate buffer에 분말시료의 0.2% $Cytolase^{TM}$ 104(cellulase hemicellulase와 pectinase의 혼합물) 첨가하여 가수분해하여 추출하는 것이었다. 증류수로 추출하거나 황산암모늄 $0{\sim}0.3$ 포화도의 분획은 목적으로 하는 PE 이외의 단백질을 제거하는 수단으로 이용할 수 있었다. 또한 5.0% Triton TX-114의 두가지 액상을 이용한 분배의 방법은 추출한 조효소로부터 TLPE와 TSPE를 분리하는 수단으로 이용할 수 있었다.

• 한국균학회지
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• 제10권4호
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• pp.165-171
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• 1982

한국산 고등 균류의 항암성분을 연구하고자 강원도에서 채집한 붉은싸리버섯 Ramaria formosa $(Fr.)Qu\acute{e}l$. 로 부터 추출, 농축, 침전시킨후, 그 침전을 원심분리하고 Visking tube를 사용하여 정제한 결과 단백질-다당체를 얻었다. 이 물질의 항암 효과를 쥐에 이식된 sarcoma 180에 대하여 시험하였으며 50 mg/kg/day의 용량으로 10일간 투여한 결과 그 종양억제율은 66%였다. 8마리중 2마리에서는 완전한 종양 퇴화를 관찰하였다. 이 항암 성분을 화학적으로 분석하여 그 다당체의 대부분은 4종의 단당으로 구성되었고 단백질 부분은 14종의 아미노산으로 구성되어 있음을 밝혔다.

• 한국축산식품학회지
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• 제33권4호
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• pp.501-507
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• 2013

Lysozyme was trapped from $2{\times}$ diluted egg white using Amberlite FPC 3500 ion exchange resin (1 g/10mL of egg white). The lysozyme bound to the resin was recovered using 0.1 N glycine-NaOH buffers, pH 9.0, containing 0.5 M NaCl. After separating lysozyme, the pH of the egg white solution was adjusted to 4.75 and centrifuged to remove interfering proteins. The supernatant was collected, added with 2.5% citric acid and 5.0% ammonium sulfate combination to precipitate egg white proteins, except for ovalbumin. After centrifugation, both supernatant (S1) and precipitant were collected. The precipitant was dissolved with 4 volumes of distilled water, and then 2.0% ammonium sulfate and 1.5% citric acid combinations added, stirred overnight in a cold room, and centrifuged. The resulting supernatant (S2) was pooled with the first supernatant (S1), desalted using an ultrafiltration unit, heat-treated at $70^{\circ}C$ for 15 min, and then centrifuged. The supernatant was collected as an ovalbumin fraction and lyophilized. The separated proteins were confirmed using Western blotting. The yield of lysozyme and ovalbumin was > 88.9% and > 97.7%, respectively, and the purity of lysozyme and ovalbumin was > 97% and 87%, respectively. The results indicated that the protocol was simple, and separated lysozyme and ovalbumin effectively.

• 한국식품저장유통학회:학술대회논문집
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• 한국식품저장유통학회 2003년도 제23차 추계총회 및 국제학술심포지움
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• pp.134.1-134
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• 2003

The purpose of this study was to investigate the release of p-hydroxybenzoic acid and other compounds from cell wall materials(CWM) and their cellulose fraction from carrot with chemical and enzymatic hydrolysis. To investigate this effect on cell wall chemistry of carrot, alcohol insoluble residue(AIR) of CWM were prepared and were extracted sequentially with water, imidazole, CDTA(-1, -2), Na$_2$CO$_3$(-1, -2), KOH(0.5, 1.0 and 4M), to leave a residue. These were analysed for their carbohydrate and phenolic acids composition. Arabinose and galactose were the main noncellulosic sugars. Phenolics esterified to cell walls in carrot were found to consist primarily of p-hydroxybenzoic acid with minor contribution from vanillin, ferulic acid and p-hydroxybenzaldehyde. p-Hydroxybenzoic acid was quite strongly bound to the cell wall. The contents of p-hydroxybenzoic acid in 0.5M KOH, Na$_2$CO$_3$-2, IM KOH, and ${\alpha}$-cellulose were 2,097, 1,360, 1,140, and 717 $\mu\textrm{g}$/g AIR from CWM, respectively. Alkali labile unknown aromatic compound(C$\sub$7/H$\sub$10/O$_2$) was found in ${\alpha}$ -cellulose hydrolyzate digested with driselase and cellulase. This compound was also found in hydrolyzate of 2 M trifluoroacetic acid at 120$^{\circ}C$ for 2 hours. Driselase treatment solubilized only 46.6 $\mu\textrm{g}$/g of the p-hydroxybenzoic acid from carrot AIR. These results indicate that p-hydroxybenzoic acid was associated with neutral polysaccharides, long chain galactose and branched arabinan from graded alcohol precipitation.

• Journal of Forest and Environmental Science
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• 제10권1호
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• pp.32-37
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• 1994

고차기생자(高次寄生者) Tetrastichus sp.는 단기생성(單寄生性) 외부기생자(外部寄生者)로 일차기생자(一次寄生者) Aneristus ceroplastae, Microterys flavus, Coccophagus hawaiiensis의 종령유충(終齡幼蟲)이나 용에 산란(産卵)하였다. 란(卵)부터 성충(成蟲)까지의 발육한계온도(發育限界溫度)와 유효적산온도(有效積算溫度)는 각(各) $9.8^{\circ}C$와 272일도(日度)로 계산(計算)되었다. $25^{\circ}C$의 항온(恒溫), 16시간(時間) 조명조건(照明條件)에서 봉밀원액(蜂蜜源液)을 급여(給與)하며 사육(飼育)한 자(雌) 성충(成蟲)의 평균수명(平均壽命)은 40일(日) 이었다. 성충(成蟲)은 우화(羽化) 24시간후(時間後)부터 산란(産卵)을 시작하여 일생(一生)동안 평균(平均) 220란(卵)을 낳았으며, 50% 누적산란율(累積産卵率)은 산란개시(産卵開始) 14일(日) 후(後)에 달성(達成)되었다. 순번식율(純繁殖率)(Ro)과 세대기간(世代期間)(T), 내적자연증가율(內的自然增加率)(r)은 각(各) 99.6/세대(世代), 32일(日), 0.142/♀/일(日) 이었다.

• Bulletin of the Korean Chemical Society
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• 제23권8호
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• pp.1139-1143
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• 2002

Analysis of lysophosphatidic acids (LPAs) is of clinical importance as they can serve a potential marker for ovarian and other gynecological cancers and obesity. It is critically important to develop a highly sensitive and specific method for the early detection of gynecological cancers to improve the overall outcome of this disease. We have established a novel quantification method of LPAs in human plasma by negative ionization tandem mass spectrometry (MS-MS) using multiple reaction monitoring (MRM) mode without the conventional TLC step. Protein-bound lipids, LPAs in plasma were extracted with methanol : chloroform (2:1) containing LPA C14:0 as an internal standard under acidic condition. Following back extraction with chloroform and water, the centrifuged lower phase was evaporated and reconstituted in methanol. The reconstituted solution was directly injected into electrospray source of MS/MS. For MRM mode, Q1 ions selected were m/z 409, 433, 435, 437 and 457 which corresponds to molecular mass [M-H]- of C16:0, C18:2, C18:1, C18:0 and C20:4 LPA, respectively. Q2 ions selected for MRM were m/z 79, phosphoryl product. Using MS/MS with MRM mode, all the species of LPAs were completely separated from plasma matrix without severe interferences. This method allowed simultaneous detection and quantification of different species of LPAs in a plasma over a linear dynamic range of 0.01-25 ㎛olL-1 . The detection limit of the method was 0.3 pmol/mL, with a correlation coefficient of 0.9983 in most LPAs analyzed. When applied to the plasmas of normal and gynecological cancer patients, this new method differentiated two different groups by way of total LPA level.

• 콘크리트학회논문집
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• 제25권2호
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• pp.145-153
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• 2013

촉진 염화물 확산계수는 최근들어 염화물 거동 평가를 위하여 많이 사용되고 있다. 촉진 염화물 확산계수는 겉보기 확산계수와 마찬가지로 재령에 따라 감소하는데, 이 연구에서는 공극률을 이용하여 촉진확산계수의 감소를 구현하였다. DUCOM 프로그램을 이용하여 15 배합에 대한 공극률을 도출하였으며, 이를 회귀분석하여 재령 270일 동안 감소하는 염화물 확산계수를 모델링하였다. 또한 자유염화물과 구속염화물간의 관계인 비선형 구속능을 고려하여, 고성능 콘크리트내의 염화물 거동을 평가하였다. 기존의 실험자료인 180일간 염화물에 침지되어 있는 시편을 이용하여, 이 연구에서 제안한 기법의 검증을 수행하였다. 제안된 기법은 다양한 물-시멘트비 및 혼화재(고로슬래그 미분말 및 플라이애쉬)를 가진 고성능 콘크리트의 염화물 거동을 적절하게 평가하였다. 또한 혼화재료를 사용한 콘크리트의 경우, 확산계수의 시간의존성이 뚜렷하므로 염화물 거동 해석시 재령에 따른 염화물 확산계수의 감소를 반드시 구현해야 함을 알 수 있었다.