• 제목/요약/키워드: Bottom Culture

검색결과 211건 처리시간 0.025초

Scanning Electronic Microscopy Examination for the Egg of Skin Parasite, Entobdella hippoglossi on the Commercially Important Culture Fish, the Atlantic Halibut, Hippoglossus hippoglossi

  • Yoon Gil Ha
    • Fisheries and Aquatic Sciences
    • /
    • 제1권1호
    • /
    • pp.68-71
    • /
    • 1998
  • The egg structure and shape of the skin parasite, Entobdella hippoglossi from the Atlantic halibut, Hippoglossus hippoglossus was examined using scanning electronic microscope. The newly produced eggs of E. hippoglossi were yellowish in color and tetrahedral in shape. The eggs were entwined together in a complicated chain-like fashion by a long filament. The appendage showed the presence of buoy-like structures. The buoy-like structures on the appendage of the eggs of E. hippoglossi in the present study were totally different from other sticky droplets. It is suggested that these structures are not sticky droplets but buoys for floating eggs which have entwined together on the bottom of the sea. It seems that these buoy-like structures may be needed for preventing the eggs from being covered by mud or particles and thus maintaining the eggs in an oxygenated environment.

  • PDF

생물반응기에서 형질전환된 식물조직 배양을 위한 기초연구 (Basic Studies on Cultivation of Transformed Plant Tissue in Bioreactor)

  • 박성화;박돈희
    • KSBB Journal
    • /
    • 제5권3호
    • /
    • pp.207-214
    • /
    • 1990
  • Growth properties of carrot hairy roots transformed by Agrobacterium rhizogenes were compared in flask and bioreactor. Oxygen transfer coefficient KLa was measured during the cultivation in bioreactor. In flask cultures, initially sucrose 30g/l was nearly exhausted after 20days. pH was dropped from initially 5.8 to 4.79 after 4 days, but it is stable after that time. Finally, after 28 days, hairy roots were grown about twelve times. In view of the results studied optimum conditions, hairy roots were maintained high growth rates in sucrose 50g/l, pH 5.8, total nitrogen 60mM. Also in bioreactor cultures, fixed stainless sieve in bottom and aerated 0.31 vvm, the results of cultivation by the use of sucrose 50g/l had grown about twenty-eight times and pH variations were liked in flask. As a results, growth rate of 1.756g fresh weight/day/g inoculum in bioreactor were higher about three times than 0.57g fresh weight/day/g inoculum in flask culture. KLa values were showed a tendency to decrease from 0.209 min-1 to 0.068 min-1.

  • PDF

폴리다이아세틸렌을 이용한 미세유동칩 내의 온도 측정 (Temperature Measurements in a Microfluidic Chip with Polydiacetylene Sensor)

  • 장영식;류성민;송시몬
    • 대한기계학회:학술대회논문집
    • /
    • 대한기계학회 2008년도 추계학술대회B
    • /
    • pp.2696-2699
    • /
    • 2008
  • Microfluidic chips have been frequently utilized to perform biochemical analysis, like cell culture, because they reduce the consumptions of analytes and reagents and automate multi-step analysis processes. It is often critical to monitor temperature in a microchannel for the analyses in order to control a reaction condition of bio or chemical molecules. We propose a novel method to monitor temperature of a microchannel flow by using polydiacetylene (PDA), a conjugated polymer, that has a unique property to transform its color from visible blue to fluorescent red by thermal stress. We inject PDA sensor droplets generated by hydrodynamic instability into a microchannel with a microheater incorporated on the channel bottom. Also, we change the channel temperature by providing the different electric power to the microheater. The results show that the florescence intensity of PDA sensor droplets linearly increases in response to the flow temperature increase within a certain range.

  • PDF

Death and the Inoperative Community in the Works of Gabriel Garcia Marquez and Merlinda Bobis

  • Prado, John Andrew M. del
    • 수완나부미
    • /
    • 제14권1호
    • /
    • pp.229-246
    • /
    • 2022
  • Gabriel García Márquez's short story "The Handsomest Drowned Man in the World" ["El ahogado más hermoso del mundo," 1968] and the novel Chronicle of a Death Foretold [Crónica de una muerte anunciada, 1981] and Merlinda Bobis's novel Fish-Hair Woman (2012) and short story "O Beautiful Co-Spirit" (2021) feature unusual scenarios of death: the arrival of a drowned man's corpse at an island; the inaction of the community to stop the foretold death of a supposedly-innocent man; a woman with long hair that can fetch dead bodies at the bottom of the village river; and a Filipino Catholic and a Malaysian Muslim working together to prepare an Italian Catholic's corpse for a funeral. These narratives demand critical attention as all deaths make the community's existence meaningful as they alter its social reality. Looking into the works of the aforementioned Colombian writer and Filipino writer and unveiling how death affects the community, this paper relies on Jean-Luc Nancy's theory on death and inoperative community.

해양 자유생활형 선충류 Enoplolaimus sp. (Enoplida: Thoracostomopsidae)와 Bathylaimus sp. (Enoplida: Tripyloididae)의 배양용 적합배지 선정 및 초기 생활사 연구 (Sutdy of Appropriate Media Selection and Early Life Cycle of Marine Free-Living Nematodes, Enoplolaimus sp. (Enoplida: Thoracostomopsidae) and Bathylaimus sp. (Enoplida: Tripyloididae))

  • 신아영;김동성;강태욱;오제혁;이지민;홍재상
    • 한국해양학회지:바다
    • /
    • 제23권3호
    • /
    • pp.109-124
    • /
    • 2018
  • 실험실 내에서의 해양 선충류 최적 배양 조건을 찾기 위해 한천배지로 다양한 배지를 제작하여 선충류를 대상으로 한 연구를 수행하였다. 선충류 채집은 태안 만리포 조간대 중 하부의 사질 퇴적물 표층에서부터 약 5 cm의 퇴적물에서 이루어졌다. 배양에 이용한 한천 배지는 Killian 배지의 조성을 다소 변형하고, 한천 농도에 차이를 두었으며, 이 한천배지들을 직경 60 mm 페트리디쉬에 배지 높이가 2.0 mm가 되도록 분주하였다. 선충류의 먹이 생물로서 순수 배양한 미생물 5종을 혼합하여 각각의 배지에 첨가하였다. 각 배지에 Enoplolaimus sp. 5개체를 접종하여 $20^{\circ}C$, 암배양으로 설정된 배양기 내에서 배양하였다. Enoplolaimus sp.에 대하여 여러 조건의 실험을 반복 수행한 결과, Killian 배지에 소고기 육즙만을 첨가한 배지의 한천농도 1.0%에서 평균 생존시간이 246.8시간으로 가장 높은 생존율을 나타냈다. 가장 낮은 생존시간은 Killian 배지에 bacto peptone과 소고기 육즙(beef extract)을 첨가한 한천농도 0.4%의 먹이 공급을 한 배지에서 평균 27.6시간으로 가장 낮은 생존시간을 나타냈다. Bathylaimus sp.는 Killian 배지 한천농도 1.0%의 먹이 공급을 하지 않은 배지에서 가장 높은 생존시간을 나타내었고, 산란량과 부화율 실험에서는 평균 7개의 알을 산란하였으며 평균 99.5시간 후에 94.7%의 부화율을 보이는 결과를 나타냈다

거제한산만 굴 양식장에 대한 GIS 기반 어장관리시스템 개발 (Development of Bivalve Culture Management System based on GIS for Oyster Aquaculture in GeojeHansan Bay)

  • 조윤식;홍석진;김형철;최우정;이원찬;이석모
    • 해양환경안전학회지
    • /
    • 제16권1호
    • /
    • pp.11-20
    • /
    • 2010
  • 굴 양식 산업은 우리나라의 대표적인 양식수산업으로서 중요한 역할을 수행하여 왔지만, 최근에는 수출 감소, 국내 내수시장 소비의 한계, 양식 환경 악화와 같은 여러 문제에 직면하여 있다. 적정하고 지속적인 굴 생산은 어장환경의 공간정보를 기반으로 하는 적지 선정을 통하여 달성 가능하고, 양식장의 적지 선정은 양식장의 성공적 개발에 중요한 열쇠이며, 지속가능한 개발에 절대적 영향을 미친다. 이 연구는 GIS 기반의 다중 평가 방법을 이용하여 수하식 굴 양식장의 적지를 규명하고자 하였다. 대부분의 인자들은 GIS에서 보간법에 의해 추출되었고, 여덟 개의 환경 인자들이 두 가지 기본 서브모델로 그룹화 되었다. 이는 굴 성장에 관련된 인자(수온, 염분, 해수유동, 클로로필 a)와 양식장 주변 환경에 관련된 인자(저층 DO, 총유기탄소, 퇴적물 산휘발성 황화물, 저서군집 다양도) 등으로 구성되어 있다. 적합 점수는 가장 적지인 8점에서부터 가장 적합하지 못한 1 점까지의 규모로 가점되었으며, 총 80.1%의 잠재 지역이 가장 높은 점수 5점과 6점으로 채점되었다. 이 지역들이 거제한산만에서 굴 양식을 위한 가장 최적의 조건을 가지는 것으로 나타났으며, 이러한 GIS 기반의 적지 선정 기법은 공간적인 의사 결정을 지원함으로서 어장관리시스템으로 활용될 수 있다.

두룽족 여성의 얼굴 문신 문화에 관한 연구 (A Study on the Chinese Dai Tattoo Culture)

  • 후어타오;임희경
    • 한국응용과학기술학회지
    • /
    • 제40권2호
    • /
    • pp.348-354
    • /
    • 2023
  • 문신은 인류에게 오랫동안 전승되어온 문화 형태다. 인류의 여러 문화권에서 전승되고 발전해온 전통적인 문신 풍습은 역사, 예술, 사회, 등 다양한 분야에서 학술적으로 중요한 연구 가치를 가진다. 두룽족은 중국 남서부 윈난(雲南)성과 근처 지역에 사는 소수 민족 중 하나로 안면문신을 위주로 하는 '얼굴문신(文面)' 풍습을 가지고 있다. 중화인민공화국이 성립된 1949년 전까지도 원시사회 형태를 유지한 두룽족의 전통적 문화는 문자가 없는 구전(口傳)의 형태로 전해 졌으나 두룽족의 신비로운 문신문화에 대한 보존자료가 없어 연구의 필요성이 요구된다. 따라서 본 연구는 근현대 문헌과 윈난성 지역 현장 탐사 두 가지 방법으로 수행되었다. 얼굴문신 풍습의 정확한 이유와 시기는 알 수 없으나 1966년 중국 문화 대혁명으로 금지되어서야 사라지게 되었다. 얼굴문신의 상징과 기능에 대해서는 현지 탐사과 근현대 학자들의 연구를 통해 크게 신앙숭배, 성년 의례, 심미 장식, 민족 사회 역사의 4가지로 나눌 수 있으며 문신을 하는 시기는 7~8세 부터이다. 얼굴문신은 지역에 따라 상류 여성은 비교적 복잡한 도안으로 구성되었으며, 하류 여성은 간단한 도안으로 구성되었다. 문신은 주로 친족이 시술하며 주로 대나무 꼬챙이와 솥바닥에서 추출한 회즙 재를 재료로 사용한다. 현재 두룽족의 얼굴문신을 한 여성은 25명 미만으로, 대부분 고령화되어 있어 수십 년 안에 모두 사라질 것으로 보인다. 따라서 두룽족의 독특한 얼굴문신 문화에 대한 문서화가 시급하다.

누란(樓蘭) 복식 연구 - 고대 한국복식과의 상관관계를 중심으로 - (A Study on the Costume of Loulan - Centered Around Interrelationship with Ancient Korean Costume -)

  • 채금석;김은경
    • 한국의상디자인학회지
    • /
    • 제18권3호
    • /
    • pp.197-211
    • /
    • 2016
  • Loulan was an ancient kingdom located on the northeast of the Taklamakan Desert, a diverging point between the north and south of Silk Road, on the east of the current Turpan, from the 2nd century BC and the 7th century A.D, absorbing and propagating culture of both sides through active trade. Meanwhile, as many previous research investigations suggest the early trade between Korea and the countries bordering on Western China, characteristics of Loulan costume have been found to have similarity to those of ancient Korean costume. Also, it can be compared to costume in other surrounding regions. Therefore, it is a highly valuable region in researching ancient costume. This study examined the historical background of Loulan and its relationship with ancient Korea, analyzed the clothes found in historic sites of Louland and Niya, and compared costume elements of Loulan and those of Korea. The result of the study shows that first, the Loulan Jeogori was based on the Jikryeonggyoim, with Chaksu, narrow sleeves and the length coming between below the chest and above the bottom, and the line decoration and waist belt were very similar to Korean costumes, as well as the Banryeong, curved collar and Bansu, short sleeves. Second, the types of pants included Gunggo, characterized by closed bottom and many creases, and Daegugo, wide-leg trousers, which are very similar in shape to the Korean Shilla lay sculpture, Stele To Martyr Yi Ch'a-don, Yangjikgongdo and Wangheedo. Third, in accessories as well, the Jowu stuck on the Gwanmo is similar in the shapes to Korean costume.

  • PDF

Ethrel의 농도, 처리시기 및 질소농도별 시비의 잎담배 성숙에 미치는 영향 (Effects of Ethrel on Tobacco-Leaf Maturity -Influences by Different Levels of the Chemical, Soil Nitrogen and Time of the Chemical Application-)

  • 정병화
    • Journal of Plant Biology
    • /
    • 제17권1호
    • /
    • pp.15-21
    • /
    • 1974
  • The most commonly grown economical and flue-cured tobacco cultivar Yellow Special A was used in pot-culture tests in order to study Ethrel (2-chloroethyl phosponic acid) effects on accelerating maturity of tobacco leaves in relation to the most adequate level of the chemical useful for field growing, nitrogen level in soil for the most pronounced response, and the most suitable spray period during the growth stages of pre-, post- and topping periods. The following conclusions, thus, were obtained from the present studies; 1. 500ppm Ethrel spray was reconfirmed to be adequate in the practical applications, although the extent of yellow-ripening of tobacco leaves was increased as the Ethrel level increased. The highest leevel treated resulted in causing chemically damaged lesions on leaves and early defoliation. 2. Ethrel-treated leaves showed deeper yellowish tinge to them than those without treatment, while different levels of the chemcial had less influence on the tinge. 3. An adequate level of nitrogen supply to plants favored the Ethrel response, whereas either very low or high level of nitrogen in the soil lowered the chemical effect on accelerating the yellow-ripening. When carbohydrates versus total nitrogen ratio became relatively high, the condition brought out some outstanding Ethrel effects. 4. Chlorophyll level of leaves increased as soil applications of nitrogen level increased, and that also increased carotenoid level of the tobacco leaves. Ethrel-treated leaves showed deeper orange tinge than those without treatment, while the highest level of nitrogen application showed the deepest orange tinge to tobacco leaves. 5. Pre-topping treatment (12 days before topping and flowering) resulted in almost no Ethrel response, and that treatment right on the day of topping, showed response of yellow-leaf ripening at nearly bottom-half leaves of a tobacco plant. The post-topping treatment (12 days after topping) made plants showing full response of Ethrel from bottom to the top leaves of tobacco plant in accelerating the leaf maturity. 6. The extent of Ethrel responses on accelerating yellow-ripening of tobacco leaves was discussed for the modifying influences brought about by certain environmental factors. Discussions were also made about the possible practical applications (particularly for pre-rice planting) and quality difference that may be caused by such growth environments.

  • PDF

남성동성애자의 성 역할에 따른 의복태도 및 선호 (The Clothing Attitude and Preference of the Gay Men According to Gender Role)

  • 이정욱;신혜원;김희라;하오선
    • 한국의류학회지
    • /
    • 제27권6호
    • /
    • pp.696-704
    • /
    • 2003
  • The main purpose of this study is to examine the gay men's clothing attitude and preference according to gender role, for reviewing their clothing culture. Specifically, this study focuses on 1) investigating gay men's clothing attitude(brand orientation, conformity, fashion leadership, sexual attractiveness), 2) identifying their clothing preference, 3) comparing the difference of lifestyle, and 4) comparing the difference of their preference color and wearing the accessary, according to their gender role. Futhermore, this study compares those factors between gay men and heterosexual men. The data was collected from 168 persons(76 gay men and 92 heterosexual men) in LGHRF(Lesbian & Gay Human Rights Federation), Korea Gaymen's Coalition and adult men living in Seoul. To analyze data, the methodology adopted in this study is frequency, t-test, and ANOVA. The results found in this study are as followings: First, sexual attractiveness was significant factor for the gay men's clothing attitude and not concern about conformity. According to the gender role, bottom, who have much of the woman in composition, was more higher for the fashion leadership than top, who have a propensity for masculine. Second, gay men prefer to masculine, simple and casual style. According to the gender role, 'top' was more likely to masculine, simple and formal style rather than 'Bottom'. Third, Gay men were much more concern about a cultural life style. Furthermore, a preference color for both group was blue. Especially, gay men tended to prefer a chromatic color. The implication getting from conclusion in this study was to study Gay men's taste for a fashion, clothing attitude and their preference to purchase, etc., in considering their buying power for clothing in a fashion market.