• KSBB Journal
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• v.5 no.3
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• pp.207-214
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• 1990

Growth properties of carrot hairy roots transformed by Agrobacterium rhizogenes were compared in flask and bioreactor. Oxygen transfer coefficient KLa was measured during the cultivation in bioreactor. In flask cultures, initially sucrose 30g/l was nearly exhausted after 20days. pH was dropped from initially 5.8 to 4.79 after 4 days, but it is stable after that time. Finally, after 28 days, hairy roots were grown about twelve times. In view of the results studied optimum conditions, hairy roots were maintained high growth rates in sucrose 50g/l, pH 5.8, total nitrogen 60mM. Also in bioreactor cultures, fixed stainless sieve in bottom and aerated 0.31 vvm, the results of cultivation by the use of sucrose 50g/l had grown about twenty-eight times and pH variations were liked in flask. As a results, growth rate of 1.756g fresh weight/day/g inoculum in bioreactor were higher about three times than 0.57g fresh weight/day/g inoculum in flask culture. KLa values were showed a tendency to decrease from 0.209 min-1 to 0.068 min-1.

• Proceedings of the KSME Conference
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• 2008.11b
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• pp.2696-2699
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• 2008

Microfluidic chips have been frequently utilized to perform biochemical analysis, like cell culture, because they reduce the consumptions of analytes and reagents and automate multi-step analysis processes. It is often critical to monitor temperature in a microchannel for the analyses in order to control a reaction condition of bio or chemical molecules. We propose a novel method to monitor temperature of a microchannel flow by using polydiacetylene (PDA), a conjugated polymer, that has a unique property to transform its color from visible blue to fluorescent red by thermal stress. We inject PDA sensor droplets generated by hydrodynamic instability into a microchannel with a microheater incorporated on the channel bottom. Also, we change the channel temperature by providing the different electric power to the microheater. The results show that the florescence intensity of PDA sensor droplets linearly increases in response to the flow temperature increase within a certain range.

• SUVANNABHUMI
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• v.14 no.1
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• pp.229-246
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• 2022

Gabriel García Márquez's short story "The Handsomest Drowned Man in the World" ["El ahogado más hermoso del mundo," 1968] and the novel Chronicle of a Death Foretold [Crónica de una muerte anunciada, 1981] and Merlinda Bobis's novel Fish-Hair Woman (2012) and short story "O Beautiful Co-Spirit" (2021) feature unusual scenarios of death: the arrival of a drowned man's corpse at an island; the inaction of the community to stop the foretold death of a supposedly-innocent man; a woman with long hair that can fetch dead bodies at the bottom of the village river; and a Filipino Catholic and a Malaysian Muslim working together to prepare an Italian Catholic's corpse for a funeral. These narratives demand critical attention as all deaths make the community's existence meaningful as they alter its social reality. Looking into the works of the aforementioned Colombian writer and Filipino writer and unveiling how death affects the community, this paper relies on Jean-Luc Nancy's theory on death and inoperative community.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.23 no.3
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• pp.109-124
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• 2018

In order to find the optimum culture condition for marine free-living nematoda in the laboratory, various agar media were developed and experiments were carried on nematodes. Nematodes have collected from the bottom of the sandy sediments' surface layer (about 5cm sediment) of Taean Mallipo beach's intertidal zone. Especially, with regard to agar medium, Killian medium was transformed slightly, density of agar had made a difference and this agar medium(height 2.0 mm on diameter 60 mm Petri dish) was divided. It was mixed with 5 different species of microorganism as nematodes' live food and added each culture medium. Five individuals of Enoplolaimus sp. on each culture medium were grown in a culture medium which was set on $20^{\circ}C$ and light blocked. Moreover, as a result of the optimum culture condition, 1.0% density of agar showed the highest survival rates (the average time of survival is 246.8 hours). On the other hand, the 0.4% density of agar adding Killian medium, bacto peptone and beef extract showed the lowest survival rates, which indicates the average time of survival is 27.6 hours. About Bathylaimus sp., on Killian medium's 1.0% density of agar(no feeding amount) showed the highest survival rates, which connects that the hatching rate 94.7% after 99.5 hours and it laid 7 eggs averagely on the spawning amount and the hatching rate experiment.

• Journal of the Korean Society of Marine Environment & Safety
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• v.16 no.1
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• pp.11-20
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• 2010

Oyster production is playing an important role in domestic aquaculture, but facing some problems such as exports decrease, a slowdown in domestic demand and marine environmental deterioration. In order to obtain the suitable and sustainable oyster production, suitable sites selection is an important step in oyster aquaculture. This study was conducted to identify the suitable sites for lunging culture of oyster using Geographic Information System(GIS)-based multi-criteria evaluation methods. Most of the parameters were extracted by Inverse Distance Weighted(IDW) methods in GIS and eight parameters were grouped into two basic sub-models for oyster aquaculture, namely oyster growth sub-model(Sea Temperature, Salinity, Hydrodynamics, Chlorophyll-a) and environment sub-model(Bottom DO, TOC, Sediment AVS, Benthic Diversity). Suitability scores were ranked on a scale from 1(leased suitable) and 8(most suitable), and about 80.1% of the total potential area had the highest scores 5 and 6. These areas were shown to have the optimum condition for oyster culture in GeojeHansan Bay. This method to identify suitable sites for oyster culture may be used to develop bivalve culture management system for supporting a decision making.

• Journal of the Korean Applied Science and Technology
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• v.40 no.2
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• pp.348-354
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• 2023

A tattoo is a cultural form that has been handed down to mankind for a long time. Traditional tattoo customs that have been handed down and developed in the various cultures of mankind have the important value of academic research in various fields such as history, art, society, etc. The Derung people, one of the minorities living in Yunnan Province and nearby areas in the southwest of China, have 'facial tattoo' customs. The traditional culture of the Derung people, which had maintained the form of primitive society until 1949 when the People's Republic of China was established, was passed down through oral tradition, so there are no preserved materials about their tattoo culture, showing the need for research on the culture. Therefore, it was conducted in two ways: a research on modern and contemporary literature and a field trip to Yunnan Province. The exact reason and time of facial tattoo customs are unknown, but the customs disappeared after being banned in 1966 due to the Chinese Cultural Revolution. The symbols and functions of facial tattoos can be largely divided into four categories, including religious worship, coming-of-age ceremonies, aesthetic decorations, and ethnic and social history, through an on-site survey and research by modern and contemporary scholars. And, it is known that women of the Derung People get tattoos from the age of 7 to 8. The design of facial tattoos became more complicated for upper-class women and simpler for lower-class women depending on the area they live. Tattoos are mainly performed by relatives, and mainly bamboo skewers and lixivium extracted from the bottom of the pot are used as the materials. Currently, there are fewer than 25 women of the Derung people with facial tattoos, and most of them are elderly. Therefore, they seem to disappear altogether within a few decades. Therefore, it is urgent to have documentation on the unique facial tattoo culture of the Derung people.

• Journal of the Korea Fashion and Costume Design Association
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• v.18 no.3
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• pp.197-211
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• 2016

Loulan was an ancient kingdom located on the northeast of the Taklamakan Desert, a diverging point between the north and south of Silk Road, on the east of the current Turpan, from the 2nd century BC and the 7th century A.D, absorbing and propagating culture of both sides through active trade. Meanwhile, as many previous research investigations suggest the early trade between Korea and the countries bordering on Western China, characteristics of Loulan costume have been found to have similarity to those of ancient Korean costume. Also, it can be compared to costume in other surrounding regions. Therefore, it is a highly valuable region in researching ancient costume. This study examined the historical background of Loulan and its relationship with ancient Korea, analyzed the clothes found in historic sites of Louland and Niya, and compared costume elements of Loulan and those of Korea. The result of the study shows that first, the Loulan Jeogori was based on the Jikryeonggyoim, with Chaksu, narrow sleeves and the length coming between below the chest and above the bottom, and the line decoration and waist belt were very similar to Korean costumes, as well as the Banryeong, curved collar and Bansu, short sleeves. Second, the types of pants included Gunggo, characterized by closed bottom and many creases, and Daegugo, wide-leg trousers, which are very similar in shape to the Korean Shilla lay sculpture, Stele To Martyr Yi Ch'a-don, Yangjikgongdo and Wangheedo. Third, in accessories as well, the Jowu stuck on the Gwanmo is similar in the shapes to Korean costume.

• Journal of Plant Biology
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• v.17 no.1
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• pp.15-21
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• 1974

The most commonly grown economical and flue-cured tobacco cultivar Yellow Special A was used in pot-culture tests in order to study Ethrel (2-chloroethyl phosponic acid) effects on accelerating maturity of tobacco leaves in relation to the most adequate level of the chemical useful for field growing, nitrogen level in soil for the most pronounced response, and the most suitable spray period during the growth stages of pre-, post- and topping periods. The following conclusions, thus, were obtained from the present studies; 1. 500ppm Ethrel spray was reconfirmed to be adequate in the practical applications, although the extent of yellow-ripening of tobacco leaves was increased as the Ethrel level increased. The highest leevel treated resulted in causing chemically damaged lesions on leaves and early defoliation. 2. Ethrel-treated leaves showed deeper yellowish tinge to them than those without treatment, while different levels of the chemcial had less influence on the tinge. 3. An adequate level of nitrogen supply to plants favored the Ethrel response, whereas either very low or high level of nitrogen in the soil lowered the chemical effect on accelerating the yellow-ripening. When carbohydrates versus total nitrogen ratio became relatively high, the condition brought out some outstanding Ethrel effects. 4. Chlorophyll level of leaves increased as soil applications of nitrogen level increased, and that also increased carotenoid level of the tobacco leaves. Ethrel-treated leaves showed deeper orange tinge than those without treatment, while the highest level of nitrogen application showed the deepest orange tinge to tobacco leaves. 5. Pre-topping treatment (12 days before topping and flowering) resulted in almost no Ethrel response, and that treatment right on the day of topping, showed response of yellow-leaf ripening at nearly bottom-half leaves of a tobacco plant. The post-topping treatment (12 days after topping) made plants showing full response of Ethrel from bottom to the top leaves of tobacco plant in accelerating the leaf maturity. 6. The extent of Ethrel responses on accelerating yellow-ripening of tobacco leaves was discussed for the modifying influences brought about by certain environmental factors. Discussions were also made about the possible practical applications (particularly for pre-rice planting) and quality difference that may be caused by such growth environments.

• Journal of the Korean Society of Clothing and Textiles
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• v.27 no.6
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• pp.696-704
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• 2003

The main purpose of this study is to examine the gay men's clothing attitude and preference according to gender role, for reviewing their clothing culture. Specifically, this study focuses on 1) investigating gay men's clothing attitude(brand orientation, conformity, fashion leadership, sexual attractiveness), 2) identifying their clothing preference, 3) comparing the difference of lifestyle, and 4) comparing the difference of their preference color and wearing the accessary, according to their gender role. Futhermore, this study compares those factors between gay men and heterosexual men. The data was collected from 168 persons(76 gay men and 92 heterosexual men) in LGHRF(Lesbian & Gay Human Rights Federation), Korea Gaymen's Coalition and adult men living in Seoul. To analyze data, the methodology adopted in this study is frequency, t-test, and ANOVA. The results found in this study are as followings: First, sexual attractiveness was significant factor for the gay men's clothing attitude and not concern about conformity. According to the gender role, bottom, who have much of the woman in composition, was more higher for the fashion leadership than top, who have a propensity for masculine. Second, gay men prefer to masculine, simple and casual style. According to the gender role, 'top' was more likely to masculine, simple and formal style rather than 'Bottom'. Third, Gay men were much more concern about a cultural life style. Furthermore, a preference color for both group was blue. Especially, gay men tended to prefer a chromatic color. The implication getting from conclusion in this study was to study Gay men's taste for a fashion, clothing attitude and their preference to purchase, etc., in considering their buying power for clothing in a fashion market.

• The Korean Journal of Malacology
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• v.17 no.1
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• pp.35-44
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• 2001

This research was conducted to develop seedling production techniques in flat oyster, Ostrea dense lamellosa. The cultivation of larvae, artificial spat and spats collection in natural conditions rate were examined. In the mass culture tank (3 tons), average growth rates of the D Shape larvae of initial shell length (153.4 ㎛) and shell height (153.4 ㎛) were 202.6% and 212.9% at 16 days and 227.1%, 241.8% at 20 days, respectively. Instantaneous death and survival rate of the larvae were 0.160 and 54.8% at 16 days and 0.057 and 43.2% at 20 days, respectively. Collection rate of flat oyster from bottom using various collectors with oyster shell, scallop shell, ark shell and PVC plates were 32.9%, 24.1%, 16.8% and 10.0%, respectively. and the greatest collecting rate was 131.9 individuals/shell (32.9%) in laid collectors on the bottom. The collecting rate of the oyster larvae were better in laid collectors on the bottom 83.8 individuals/collection than in the suspended string 54.2 individuals/collection. in all collecting substrates. Early spats settled on collectors were grew to 2.38 $\pm$ 0.97 ㎜ in shell length and 2.16 $\pm$ 0.86 ㎜ In shell height at 26 days. The spats were grew to 28.58 $\pm$ 2.39 ㎜ in shell length and 31.65 $\pm$ 2.03 ㎜ in shell height during the 4 month mid-term cultivation. In the period of cultivation, the mean number of spats attached to collectors were 10.3 individuals at oyster shells, 5.8 scallop shells, 4.0 ark shells and 1.5 PVC plates, respectively.