• Journal of Microbiology
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• v.35 no.3
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• pp.159-164
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• 1997

The phylogenetic study of Penicilium chrysogenum was performed based on amino acid sequence comparison of chitin synthase. Phylogenetic trees were constructed with the deduced amino acid sequences of the highly conserved region of chitin synthease gene fragments amplified by PCR. The BlasP similarity searcch and the bootstrap analysis of the deduced amino acid sequences of chitin synthase from P. chrysogenum with those form other fungi showed a close evolutionary relationship of Penicillium to ascomycetous fungi, especially to genus Aspergilus. The result from bootstrap analysis of the deduced amino acid sequences of the Class II chitin synthase from ascomyceteous fungi supported the usefulness of the Class II chitin synthease for phylogenetic study of filamentous fungi.

• Journal of the Korean Data and Information Science Society
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• v.18 no.1
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• pp.175-184
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• 2007

LOD scores and a permutation test for detecting and locating quantitative trait loci (QTL) from the Hanwoo economic trait have been described and we selected a considerable major BMS1167 locus for further analysis. K-means clustering analysis, for the major DNA marker mining of BMS1167 microsatellite loci in Hanwoo chromosome17, has been tried and three cluster groups divide four traits. The three cluster groups are classified according to eight DNA marker bps. Finally, we employed the bootstrap test method to calculate confidence intervals using the resampling method to find major DNA markers. We conclude that the major marker of BMS1167 locus in Hanwoo chromosome17 is only DNA marker 100bp.

• Journal of Life Science
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• v.12 no.6
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• pp.821-834
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• 2002

In order to measure the inter- and intraspecific genetic divergences within the genus Alexandrium, the variations within the internal transcribed spacer (ITS1 and ITS2) regions and 5.85 ribosomal RNA gene of eight Alexandrium species were examined for 33 strains from diverse geographical locations by direct sequencing. Five isolates of A. tamarense (AT-2, AT-6, AT-10, AT-A and AT-B) from Jinhae Bay, Korea were found to be completely identical to a Japanese strain OFX151-A. The length of the amplified ITSI-5.85-ITS2 region varied from 481 nucleotides (in A. margalefi) to 528 nucleotides (in A. affine CU1-1). ITS1 and ITS2 nucleotide lengths were negatively correlated, whereas a positive correlation was found between their G＋C content. The degree of sequence divergence ranged from 0.3% (1 bp) to a maximum of 53% (305 Up). Pairwise sequence comparisons revealed a small degree of divergence between A. tamarense and A. Pundyense isolates (1.2 - 2.3% ＝ 6-12 bp), but a high degree of divergence between A. tamarense and A. catenella (19.8% ＝ 102 bp), and between A. catenella and A. Pundyense (19.7%). Although most nodes were weakly supported by bootstrap values, some types tend to form independent molecular groups. A. catenella isolates also formed an independent molecular sub-group, with relaticula strong bootstrap values (94% or 85% and 79% or 98%, respectively in PAUP and NJ trees). Interestingly, A. cohorticula and A. frateculus always clustered within the same sub-group, this result being supported by strong bootstrap values. Our results indicate that the ITS regions provide useful informations on hierarchical population genetic structure and a high phylogenetic resolution in intraspecific and interspecific Alexandrium population.

• Proceedings of the Korea Water Resources Association Conference
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• 2009.05a
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• pp.1157-1161
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• 2009

본 연구에서는 부트스트랩(Bootstrap) 기법을 이용하여 측우기 강우량 관측계열(CWK)과 근대우량계 강우량 관측계열(MRG)에 대해 동질성 분석을 실시하였다. 서로다른 두 자료계열에 대한 전통적인 통계적 동질성 검정 방법은 모집단의 분포형을 알고 있어야 검정결과가 유효하였기 때문에 모집단의 분포가 복잡한 기상자료들은 이러한 전통적 방법을 사용하여 동질성을 파악하는 것이 매우 어려웠고 결과로 제시된 통계적 유의성에 대해서도 의심의 여지가 있었다. 이러한 이유로 본 논문에서는 모집단을 가정하지 않아도 되는 비모수적 모의 방법인 부트스트랩 기법을 이용하여 두 자료계열간의 동질성 검정을 실시하였다. 분석 결과 M20의 CWK와 MRG는 미소한 기후의 경년변화 (Trend)의 영향을 제외하면 동질성을 가진 자료로 볼 수 있었으나, 갈수기의 경우는 월강우량의 크기에 변화가 있으며 호우기의 경우는 일강우량의 크기 및 호우의 형태에 변화가 있는 것으로 나타났다.

• Proceedings of the Korea Water Resources Association Conference
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• 2004.05b
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• pp.863-869
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• 2004

댐의 수리${\cdot}$수문학적 월류 확률 추정시에 가장 민감한 불확실성 변량은 댐의 초기수위라 할 수 있으며, 특히 자료의 특성을 충분히 반영하고 댐마루(dam crest)의 높이를 초과하지 않으면서 경계를 갖는 분포형을 추정하는 것은 무엇보다 중요하다. 그러나 기존의 매개변수적 확률분포 추정방법으로 이러한 문제점을 적절히 반영할 수 없으며 통계특성을 반영하지 못하고 이상화시키는 단점이 있다. 이러한 문제점을 보완하기 위해서 비매개변수적 핵밀도함수 방법과 Bootstrap 기법을 적용하여 수위의 신뢰구간을 추정하였다. 연 최대치 자료를 이용한 비매개변수적 핵밀도함수 기법을 이용한 해석결과에서는 댐의 설계빈도를 상회하는 비교적 큰 위험도 나타냈으며 홍수기의 평상수위고 가정하는 Bootstrap Resampling을 적용한 위험도는 5.11E-06의 간을 나타났다. 가장 극심한 기상상태를 가정한 해석 결과인 1.1972E-03은 본 댐은 여수로의 설계빈도가 1,000년 빈도로서 설계당시보다 확률수문량이 크게 증가된 현재 여수로 방류능력 및 안전성 상태로 고려해보면 적당한 위험도 값으로 추정된다.

• Korean Journal of Plant Taxonomy
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• v.36 no.2
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• pp.91-108
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• 2006

Phylogenetic analyses were conducted to evaluate evolution and relationship of 16 taxa of Korean Geranium including 3 outgroups using ITS (internal transcribed spacer) squences of nuclear ribosomal DNA. Phylogenetic studies used most parsimony and neighbor-joining methods including bootstrapping and jackknifing analysis. As the result, Korean Geranium forms monophyletic group. In the parsimony tree G. koraiense var. hallasanense situated as the most basal clade and Erianthum group forms one clade by high bootstrap ans jackknife values (100% of bootstrap and jackknife values). G.dahuricum as one of the Krameri group is closely related with Palustre group by very weak relationship (37% of bootstrap and 44% of jackknife values) and the node collapse in the strict tree. G. Knuthii which was one of wilfordii group is closely related with Koreanum group. G. sibiricum, one of Sibiricum group, is the most closest relationship with G. soboliferum and these species are sister to G. krameri. G. tripartitum and G. wilfordii which are wilfordii group are linked to G. nepalense, G. thunbergii f. pallidum and G. thunbergii. This result suggested that the phylogenetic analysis of ITS sequences should be useful to address phylogenetic questions on the genus Korean Geranium.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.25 no.1
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• pp.34-39
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• 2013

In this study, the quantitative analysis and pattern analysis of the error bounds with respect to recording period were carried out using the wave climate data from coastal areas. Arbitrary recording periods were randomly sampled from one month to six years using the bootstrap method. Based on the analysis, for recording periods less than one year, it was found that the error bounds decreased rapidly as the recording period increased. Meanwhile, the error bounds were found to decrease more slowly for recording periods longer than one year. Assuming the absolute estimate error to be around 10% (${\pm}0.1m$) for an one meter significant wave height condition, the minimum recording period for reaching the estimate error for Sokcho and Geoje-Hongdo stations satisfied this condition with over two years of data, while Anmado station was found to satisfy this condition when using observational data of over three years. The confidence intervals of the significant wave height clearly show an increasing pattern when the percentile value of the wave height increases. Whereas, the confidence intervals of the mean wave period are nearly constant, at around 0.5 seconds except for the tail regions, i.e., 2.5- and 97.5-percentile values. The error bounds for 97.5-percentile values of the wave height necessary for harbor tranquility analysis were found to be 0.75 m, 0.5 m, and 1.2 m in Sokcho, Geoje-Hongdo, and Anmado, respectively.

• Journal of Life Science
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• v.16 no.5
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• pp.812-827
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• 2006

To investigate the population structure and geographic distance among anchovies (Engraulis japonicus) in Korea, we compared and analyzed the mitochondrial DNA control region sequences (227 bp) of anchovies from 12 localities in inshore and offshore waters. The sequence analysis of 84 individuals showed 29 haplotypes, ranging in sequence divergence by pairwise comparisons from 0.3% to 3.5% (1 bp-12 bp). E9 haplotype of anchovies were found largely in inshore waters and also in offshore waters, which was regarded as the major source in Korean waters (58.3%). However, E26, E27, E28, and E29 haplotypes were found in westsouthern (locality 10, four among 7). Phylogenetic analysis using PHYLIP was divided into two clades (clade A and B). Most of the haplotypes, excluding E26, E27, E28, and E29, were strongly supported by bootstrap analysis (>75%), whereas the relationship between clade A and B was weakly supported by bootstrap analysis (51%). High levels of genetic diversity were found; haplotype diversity (H)=0.75-1.00, and nucleotide diversity $({\pi})=0.015-0.0244$. Analysis of $F_{ST}$ between populations in inshore waters ranged in 0.01-0.05, whereas those of offshore waters ranged in 0.01-0.58. A high gene flow occurred in inshore (Nm=22.61-34.22) and offshore (Nm=11.57-45.67) populations. The distribution of mitochondrial DNA haplotypes between westsouthern and other populations was suggestive of significantly different differentiation ($F_{ST}$=0.20-0.59, p<0.05; d=0.52, p=0.00; ${\phi}=0.02-0.41$, p＜0.05). These results suggested that the overall anchovy population in the Korean peninsula caused considerable migration due to the mitochondrial gene flow between inshore and offshore populations to form a genetically homogenous and panmictic structure, although a heterogeneous population was found in this study.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.41 no.3
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• pp.61-71
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• 2018

The purpose of this study is to analyze the efficiencies of project management offices in large information system construction projects using the data envelopment analysis. In addition, we tried to estimate the confidence interval of those efficiencies using bootstrap DEA to give a statistical meaning. The efficiency by the CCR model is analyzed as eight project management offices are fully efficient and 22 project management offices are inefficient. On the other hand, there are 15 project management offices are fully efficient, but 15 project management offices are inefficient in the BCC model. As the result of the scale efficiencies, of the inefficient project management offices, 13 project management offices are inefficient in scale. It is possible to eliminate the inefficiency in the CCR model by improving their project performances. And, the nine project management offices showed that the inefficiency was due to pure technical efficiency, and these companies should look for various improvements such as improvement of project execution system and project management process. In order that the inefficient project management offices be efficient, it is analyzed that more efforts must be made for on-budget and on-time as a result of examining the potential improvement potentials of inefficient project management offices.

• Journal of Microbiology
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• v.41 no.3
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• pp.232-238
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• 2003

Previous phylogenetic studies on human immunodeficiency virus type 1 (HIV-1) isolated from Korean patients suggest that the major subtype of Korean isolate is subtype B. In this subtype, some of the Korean isolates seem to be clustered exclusively of foreign isolates. Presence of this so-called “Korean clade” among Korean isolates is unique but needs verification since the number of Korean isolates used in previous studies was limited. This study aimed to identify the presence of the “Korean clade” by molecular phylogenetic analysis using all the Korean nef gene sequences registered in the NCBI GenBank (N=243) together with 32 reference strains and 77 foreign isolates. Extensive analysis of the nef gene nucleotide sequences by neighbor-joining method revealed the following. Most (83.1 ％) of the Korean isolates belonged to subtype B, and 81.2％ of subtype B were clustered together and excluded foreign isolates (bootstrap value=91.9％ ). Within Korean subtype B cluster, no characteristic subcluster formation was evident since the bootstrap values for the subcluster were very low. Due to limited information, the phylogenetic analysis failed to identify the epidemiological linkage among specific groups such as homosexuals and hemophiliacs within the Korean subtype B cluster. Detailed analysis and epidemiological information are needed to clarify the origin and significance of the Korean subtype B cluster.