• Korean Journal of Medicinal Crop Science
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• v.1 no.2
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• pp.97-103
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• 1993

Relationships between crown diameter divided into the small$(0.3{\sim}0.5cm)$, the medium$(0.5{\sim}0.7cm)$ and the large seedling$(0.7{\sim}0.9cm)$ by the size of crown diameter of Angelica gigas and topdressed nitrogen level of 0, 50, 70 and 100% were conducted to study on growth and yield of two year-planted A. gigas in Suwon. The larger of the crown diameter, the better of the dry weight of leaf and root of A. gigas. Growth of the plane was rapidly increased on June and August, but slowly increased on July and that of root was increased until October while decreasing the aerial stem growth from September. Lower top dressed nitrogen level in the larger seeding was greatly increased the bolting rate compared to the medium or small seedlings. Yields of dried root weight per 10a were 441kg for the large, 373kg for the medium, and 378kg for the small seedling, and weight of the higher nitrogen per 10a were more increased than that of the lower dressed nitrogen treatment.

• Journal of Plant Biotechnology
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• v.41 no.1
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• pp.56-63
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• 2014

Using commercial radish varieties for processing, about 30% of radish was discarded due to the root shape and low purity. To raise the processing ability, we tried to develop a new variety producing H-shaped root. As another characteristic required in variety for processing is high purity, we tried to raise purity using simple sequence repeats (SSR) markers for testing seed purity in every segregating generation. To develop Male-sterile (MS) seeding parent, we crossed commercial variety of 'Gwan dong spring' and 'Gyeo ryong spring'. One elite inbred was selected as recurrent parent for the MS plant. The major horticultural traits of selected inbred line were disease resistance, late bolting, heat resistance and bright green root top color. To develop pollen parent, we crossed commercial variety of 'Tae sang king' and 'Seoul spring'. We used individual selection method to develop H-shaped hard root and disease resistant inbred. In each segregating generation, we selected one plant based on phenotype and the uniformity of selected plant was tested by SSR markers using self-pollinated seeds. In the first segregating generation, 64.6% of sib plants shared the same band in PCR amplification using ACMP-490 primer and 66.7% using cnu-316 primer. The uniformity of segregating generations using ACMP-490 and cnu-316 raised in second generation to 68.8%, 70.8%, respectively; in third generation to 93.8%, 100%; in fourth generation to 93.8%, 100%; in fifth generation to 95.8%, 100%; in sixth generation to 100%, 100%. A novel cross was made using selected MS parent and pollen parent. When we checker the horticultural traits using autumn cultivation, the novel cross variety produced H-shaped root comparing other commercial varieties and produced highly uniform radish. Thus we registered this novel cross variety as 'YR ORE' at 2013 (Registration No. 4550).

• FLOWER RESEARCH JOURNAL
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• v.19 no.1
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• pp.22-29
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• 2011

The experiment investigated effect of duration, temperature, and light condition during chilling treatment on growth and flowering of four Campanula species in a factorial experiment. Two parent species, Campanula punctata Lam. var. rubriflora Mak. and C. Punctata Lam., and their two $F_1$ hybrids, C. punctata Lam. ${\times}$ C. punctata Lam. var. rubriflora Mak. ('Jiknyeo') and C. punctata Lam. var. rubriflora Mak. ${\times}$ C. punctata Lam. ('Gyeonu'), were used. Plants were cultured in vitro for five weeks at $25^{\circ}C$ under about $75{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD before being chilled at 4 or $25^{\circ}C$ for 3, 6, or 9 weeks under a darkened or lighted (about $10{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD) condition. After chilling treatment, plants were transplanted to 10 cm pots filled with a commercial growing medium and were transferred to environment-controlled growth chambers and subsequently to a greenhouse to observe their reproductive growth. Growth of all species and flowering of a $F_1$ hybrid 'Jiknyeo' were affected by duration, temperature, and light condition during chilling treatment. The greatest growth and survival percentage were observed in C. punctata Lam. var. rubriflora Mak. The survival percentage was greater when plants were chilled in a lighted than darkened condition, whereas it decreased when plants were chilled more than six weeks in vitro. Among the four species tested, flowering was observed only in a $F_1$ hybrid 'Jiknyeo' with 62.5% flowering plants when it was chilled at $25^{\circ}C$ for three weeks under a lighted condition. Percent flowering plant was affected by duration, temperature, and light condition during chilling treatment. Three-week chilling at $4^{\circ}C$ under a darkened condition significantly reduced days to flowering. These results suggest that the low temperature requirement for flowering is not qualitative but quantitative in Campanula species. Further experiment with more number of plants is necessary to ascertain this conclusion.

• Journal of Life Science
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• v.31 no.5
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• pp.511-519
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• 2021

The Perilla frutescens var. japonica HARA is widely cultivated in Korea for vegetable leaves and oil seeds. Perilla species have been used for food and medicine and are known to contain many functional compounds. In this study, we investigated the functional compound contents of Perilla during its growth stages to analyze the optimal harvest time and conditions. The contents of the Perilla sprouts were analyzed according to culture environment and days of growth. Sprouts grown in soil under natural light conditions showed high rosmarinic acid (23.19±0.16 mg/g) and GABA (0.55±0.05 mg/g) content. Therefore, the results suggest that 6 to 8 days after sowing in soil under natural light conditions was the optimum harvest condition for sprouts. Also, the functional compounds of Perilla were analyzed according to growth stage and plant part. As a result, caffeic acid and rosmarinic acid exhibited the highest content in the stage from vegetative growth to reproductive growth (0.28±0.03 ~ 0.30±0.07 mg/g rosmarinic acid and 20.60±7.02 ~ 19.37±3.18 mg/g caffeic acid), and luteolin and GABA showed the highest content in the reproductive growth stage and in the early stages of vegetative growth, respectively (31.11±2.98 ~ 22.35±1.64 ㎍/g luteolin and 0.42±0.09 ~ 0.37±0.04 mg/g GABA). It was confirmed that the content of caffeic acid (0.34±0.03 mg/g), rosmarinic acid (55.22±9.33 mg/g) and luteolin (1,044.89±6.72 ㎍/g) was the highest during the bolting stage. Overall, we identified the timing of the highest level of functional compounds in the sprouts and mature leaves of Perilla. These results suggest a suitable harvest time and conditions for sprouts and leaves for the use of Perilla as a functional material.