Objective: This study was performed to investigate the effects of external tooth bleaching with flouridation on the appearance of white spot lesions (WSLs) in vitro. Methods: In total, 125 bracket-bonded bovine incisor enamel blocks with artificial WSLs were randomly divided into a control group and four treatment groups (home bleaching, home bleaching + fluoridation, in-office bleaching, and in-office bleaching + fluoridation). A spectroradiometer (SR) and digital images (DIs) were used to evaluate colorimetric parameters (Commission Internationale l'Eclairage $L^*a^*b^*$) for all specimens. Color measurements were obtained before WSL formation (T1), after WSL formation (T2), and after completion of the external tooth bleaching treatment (T3). Results: The SRbased color change after bleaching was significantly greater in the treatment groups than in the control group (p < 0.05). SR-based lightness ($L^*$) and redgreen ($a^*$) values were significantly higher at T2 than at T1 (p < 0.001), with no significant changes in yellow-blue ($b^*$) values. At T3, SR-based $L^*$ values had increased while $a^*$ and $b^*$ values had decreased in the treatment groups (p < 0.001). The DI-based color difference between the sound enamel and WSL areas (DE*DI) increased significantly from T1 to T2 in all groups (p < 0.001) and significantly decreased from T2 to T3 in the treatment groups (p < 0.001). No significant differences in ${\Delta}E^*DI$ at T3 were shown between the four treatment groups (p > 0.05). Conclusions: The findings of this study suggested that external tooth bleaching with fluoridation can alleviate the conspicuity of WSLs.
Journal of the korean academy of Pediatric Dentistry
/
v.24
no.1
/
pp.41-57
/
1997
The present study investigated the effects of hyperbaric oxygen therapy on periodontal wound healing of replanted rat tooth. 80 rats (Sprague-Dawley strain) weighting $130{\pm}5gm$ were selected and divided into experimental and control group, each group consisting of 40 rats. Rats were administered 0.4% ${\beta}$-aminoproprionitrile for 5 days to achieve gentle tooth extraction. The maxillary first molars were extracted under anesthesia with pentobarbital, washed in sterile distilled water, treated with bacterial collagenase to remove collagen fibers on the root surfaces. After washing in water overnight, the mesial root surface were demineralized by application of citric acid, washed, dried and stored at $4^{\circ}C$. Immediately after tooth extraction and bleeding control, the treated molars extracted previously from other rats were replanted. The experimental group was exposed to hyperbaric oxygen at 2.5 atm. for 2 hrs. a day during experimental period. Eight animals of each group were sacrificed 1, 3, 6, 8, 10 days after reimplantation of teeth by intracardiac perfusion with 4% paraformaldehyde. The replanted molars and surrounding tissues were cut, demineralized, dehydrated and embedded in paraffin. Sections were stained with azan, toluidine blue and hematoxylin. Some other sections were stained by means of immunostaining achieved by the avidinbiotin complex method. The results as follows; 1. Experimental group showed fast healing of gingival epithelium. 2. Macrophage and newly formed blood vessels appeared early in the gingival connective tissue of experimental group. 3. Experimental group showed fast, abundant fibroblast proliferation and regularity of collagen fiber. 4. In both group, collagen was distributed along the collagen fiber. The distribution was strong and regular in the experimental group. 5. In the regenerated periodontal ligament of experimental group, fibers showed regular arrangement and invaded root surface fast.
Objective: Preservation of the periodontal ligament (PDL) is vital to the success of tooth autotransplantation (TAT). Increased PDL volumes and facilitated tooth extraction have been observed upon orthodontic preloading. However, it is unclear whether any changes occur in the expressions of bone biomolecules in the increased PDL volumes. This study aimed to determine the expressions of runt-related transcription factor 2 (RUNX2), alkaline phosphatase (ALP), receptor activator of nuclear factor kappa-B ligand (RANKL), and osteoprotegerin (OPG) in PDL upon preloading. Methods: Seventy-two premolars from 18 patients were randomly assigned to experimental groups that received a leveling force for 1, 2, or 4 weeks or to a control unloaded group. Following extraction, PDL volumes from 32 premolars of eight patients (21.0 ± 3.8 years) were evaluated using toluidine blue staining. The expressions of the biomolecules in the PDL from 40 premolars of ten patients (21.4 ± 4.0 years) were analyzed via immunoblotting. Results: The median percentage of stained PDL was significantly higher at 2 and 4 weeks after preloading than in the unloaded condition (p < 0.05). The median RUNX2 and ALP expression levels were significantly higher at 2 and 4 weeks after preloading than in the unloaded condition (p < 0.05), whereas the median RANKL/OPG ratios were significantly higher at 1 and 4 weeks after preloading (p < 0.05). Conclusions: Orthodontic preloading for 4 weeks enhances PDL volumes as well as the expressions of RUNX2, ALP and the RANKL/OPG ratio in the PDL, suggesting this loading period is suitable for successful TAT.
The purpose of this study is to evaluate the reliability of repeated measurements of several dental scanners. Blue-lighted scanners, white-light scanners and optical-type scanners are used in the study of repeatability in this study. The measurement results were calculated as root mean square (RMS) and the significance level was confirmed by applying the 1-way ANOVA statistical technique (𝛼=.05). According to the statistical analysis, the scanner with the largest RMS value was Z-opt group (38.2 ㎛. Next, D-white was 35.2 ㎛ and the group with the lowest RMS value was I-blue (34.1 ㎛). The comparison of RMS means between each group was not significant (p>.05). From this result, the blue light had the lowest error in repeatability of dental scanners, but no statistical significance. The conclusion of this study is that the study results are clinically acceptable.
Journal of the korean academy of Pediatric Dentistry
/
v.25
no.1
/
pp.103-115
/
1998
Adhesion of composite resin to tooth structure has been of tremendous signgicance in clinical dentistry. Due to the lack of adhesion between composite restorative resins and enamel and dentin, microleakage occurs at the tooth/restoration interface. This may lead to discoloration, secondary caries, marginal breakdown, postoperative sensitivity, and even pulpal pathology. According to extensive use of composite resin, every effort on improving bonding strength and reducing microleakage between a tooth and composite resin has been continued. This study was conducted to determine the difference in microleakage in enamel and dentin treated with air-abrasion, acid etching and combination when restored with composite resin. Class V cavities were prepared on 30 premolars. The specimens were divided into following groups. group 1:air-abrasion+Scotchbond Multi-purpose group 4 :air-abrasion+All-Bond 2 group 2:acid etching+Scotchbond Multi-purpose group 5 :acid etching+All-Bond 2 group 3:combination+Scotchbond Multi-purpose group 6 :combination+All-Bond 2 #combination:air-abrasion + acid etching The specimens were filled with Z-100 after application of Scotchbond Multi-purpose and All-Bond 2. Thermocycling was conducted by alternately dipping the specimens in $5^{\circ}C$ and $55^{\circ}C$ water for 30 seconds 500 times. 1% methylene blue was applied and the specimens were left for 24 hours at $37^{\circ}C$. After washing out the dye, the tooth was sectioned buccolingually along the axis. The sectioned surface was observed with stereoscope for dye penetration. The author has measured the microleakage in teeth prepared with air-abrasion, acid ethching and combination to study the difference in microleakage following different methods of tooth surface treatment and has come to following results. 1. In comparing microleakage between groups, group 1 and 4 showed statistically significant difference from group 2, 3, 5 and 6(p<0.05). There was no significant difference among group 2, 3, 5, 6(p>0.05) nor between group 1 and 4(p>0.05). 2. In comparing microleakage among tooth surface treatment methods, Air-abrasion group showed significantly more microleakage than acid etching group and combination(airabrasion + acid etching) group(p<0.05). Combination(acid etching+air-abrasion)group tended to show lesser microleakage than acid etching group, but this was not statistically significant(p>0.05). 3. In comparing microleakage between bonding agents, there was no statistically significant difference between Scotch bond Multi-purpose and All-Bond 2(p>0.05).
Purpose: The purpose of this study was to evaluate the validity of digital models fabricated by difference optical source of non-contact 3D dental scanner. Methods: A master model with the prepared upper full arch tooth was used. Stone model(N=10) were produced from master model, and on the other hands, digital models were made with the 3D dental scanner(Blue, white, red optical source). The linear distance between the reference points were measured and analyzed on the Delcam $Copycad^{(R)}$ graphic software. The results were statistically analyzed using the one-way ANOVA and Tukey's HSD test(${\alpha}=0.05$). Results: There were considerable differences in mean values between optical source within each color(blue, white, red), and this difference was not statistically significant(p>0.05). Conclusion : Three different color of dental scanner optical source showed clinically acceptable accuracies of full arch digital model produced by them. Besides, these results will have to be confirmed in further clinical studies.
The intact dental pulps which were free of their tooth bud from adult rat incisors, and oral mucosa were transplanted subcutaneously in homologous rats to study the formation of calcified tissue. The rat were sacrificed after 1,2,3 and 4 weeks following transplantation of dental pulp and oral mucosa. The samples which contained the transplanted and surrounding tissue were fixed in 10% NBF, stained with hematoxylin and eosin, alizarin red S, von Kossa, and alcian blue. Microscopic examinstins revealed as follows: 1. The transplanted oral mucosas were not calcified but tended to form the epithelial cysts. 2. At 1 week after transplantation of dental pulp the calcified structures were appeared at the periphery of the transplantation of dental pulp but weakly reacted to alizarin red S, von Kossa, and alcian blue. 3. At 2 weeks after transplantation of dental pulp the calcified structures began to expand from the periphery to the center of the transplanted dental pulp and occupied the large areas comparatively, and strongly reacted to alizarin red S, and von Kossa stains. 4. At 3 weeks after transplantation of pulp tissue the fibrous components were grown at the periphery of the transplanted pulp tissuesand at 4 weeks a large amount of fibrous tissues were observed. The transplanted pulp tissue tended to form foreign bodies gradually.
Journal of Dental Rehabilitation and Applied Science
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v.19
no.2
/
pp.69-74
/
2003
The purpose of this study was to obtain the color change information for natural tooth of all age groups. Fifty Korean subjects(25 men and 25 women) were randomly selected for this study. They were ranged in age from 24 to 67 years old and were classified into 5 age groups for each sex: 20 to 29, 30 to 39, 40 to 49, 50 to 59, 60 to 69 years. Maxillary central incisor of each subject free from obvious discoloration and restorations on the sites to be measured was selected. Three sites on the labial surface, incisal, middle, cervical area were measured and a circular area of 1.0mm in diameter was measured at each site by the spectrophotometer. The obtained results of this study were as follows: 1) Natural tooth color showed a significant decrease in linghtness($L^*$) at the cervical site(P<0.05). 2) Red/green chromaticity($a^*$) showed a significant increase at all sites(P<0.01). But it was not enough to have a statistic significance among three sites by aging. 3) Yellow/blue chromaticity($b^*$) showed an increase at all sites(P<0.05). But it didn't change significantly among three sites by aging.
Amalgam is one of the most commonly used dental restoration material because of its convenience, economic and physical properties. But microleakage in the tooth and amalgam interface has been its major problem, and many efforts have been made to overcome this shortcoming. The purpose of this study is to compare the effect of various liners on microleakage of amalgam restoration. Cavities were prepared on the buccal or lingual surface of ninety sound, extracted human premolars and six different liners (Tubulitec$^{(R)}$, Superbond D-liner II Plus$^{(R)}$, Superbond D-liner II Plus$^{(R)}$ with polymer, Scotchbond Multipurpose Plus$^{(R)}$, Copalite$^{(R)}$, No liner) were unapplied according to manufacturer's instructions and amalgam had been condensed immediately. The specimens were thermocycled by dipping in methylene blue dye at $5^{\circ}C$ and $55^{\circ}C$ for 1500 cycles. The amalgam-tooth interface was examined under stereobinocular microscope and the dye penetration was scored. The results were as follows : 1. The Tubulitec$^{(R)}$ group showed less microleakage than no liner or Copalite$^{(R)}$ group (p<0.01). 2. The Tubulitec$^{(R)}$, Superbond D-liner II Plus$^{(R)}$, Superbond D-liner II Plus$^{(R)}$ with polymer and Scotch bond Multipurpose Plus$^{(R)}$ groups were not significantly different. 3. The Copalite$^{(R)}$ and Scotchbond Multipurpose Plus$^{(R)}$ groups were not significantly different. Using the polystyrene liner and resin liners under admixed type of high-copper amalgam restoration significantly reduced microleakage in the tooth-amalgam interface. Further clinical studies on polystyrene liner are recommended.
Journal of the Korea Academia-Industrial cooperation Society
/
v.12
no.12
/
pp.5692-5699
/
2011
This study aims to determine potential differences in self-perceived oral health levels between blue-collar and white-collar workers. It is based upon questionnaire survey data dating from Mar. 1 to 30, 2011. All the data collected from 200 blue-collar workers and 100 white-collar worker was analyzed using statistical tools like SPSS ver. 19.0. Self-perceived oral health levels were analysed across three sub-factors - physical, psycho-social, and psychological factors - which were confirmed through factor analysis. The psycho-social factor was statistically significant for age and shift work. Self-perceived oral health levels across the three factors were quantitatively correlated except for psychological factors in blue-collar workers. The study found that occupational group affects workers' self-perceived oral health level. The explanatory power of these 4 variables total 51% in blue-collar group. In case of white-collar group, it was found one variable total 30%. Since oral health levels differ between blue-collar and white-collar workers, oral health promotion projects should differentiate between the two groups, and workers with an interest in their tooth for the prevention, yet negligent act because it will keep your personal oral health care in the prevention-oriented oral health promotion.
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