• Biomolecules & Therapeutics
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• 제9권4호
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• pp.285-290
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• 2001

Bioequivalence of two acetyl-1-carnitine tablets, test product (Carnitile tablet: Hanmi Pharm. Co., Ltd.) and reference product (Nicetil $e^{R}$ tablet: Dong-A Pharm. Co., Ltd.), was evaluated according to the guide- lines of Korea Food and Drug Administration (KFDA). Twenty-six healthy volunteers were divided randomly into two groups and administered the drug orally at the dose of 500 mg as acetyl-1-carnitine in a 2$\times$2 crossover study. Blood samples were taken at predetermined time intervals for 12 hours and the plasma concentration of acetyl-1-carnitine was determined using HPLC by derivatization with p-bromophenacyl bromide. The pearmacokinetic parameters (AU $C_{0-}$12h/ $C_{max}$ and $T_{max}$) were calculated and ANOVA was utilized for the statistical analysis of parameters. The apparent differences of these parameters between two drugs were less than 20% (i.e., 1.26,-5.08 and 8.59% for AU $C_{0-}$12h/ $C_{max}$ and $T_{max}$, respectively). The powers (1-$\beta$) for AU $C_{0-}$12h/ $C_{max}$ and $T_{max}$, and Tmax were over 0.9. Minimal detectable difference ($\Delta$) at $\alpha$=0.05, 1-$\beta$=0.8 were less than 20% (i.e.,7.31, 14.88 and 11.77% for AU $C_{0-}$12h/ $C_{max}$ and $T_{max}$, respectively). The confidence intervals ($\delta$) for these parameters were also within $\pm$ 20% (i.e.,-3.03$\leq$$\delta$$\leq$5.54, -13.80$\leq$$\delta$$\leq$3.64 and 1.69$\leq$$\delta$$\leq$15.48 for AU $C_{0-}$12h/ $C_{max}$ and $T_{max}$, respectively). These results satisfied the criteria of KFDA guideline for bioequivalence, indicating Carnitile bioequivalent to Nicetil $e^{R}$ .TEX>$^{R}$ .> R/ . R/ .

• Human Ecology Research
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• 제56권5호
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• pp.417-424
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• 2018

The use stage of a textile product impacts sustainability more significantly than other stages of the product's life cycle due to repeated washing and drying. This study determines efficient washing conditions, with high detergency, to reduce energy consumption from excessive washing and improve the washing process sustainability. Detergency was measured at various washing temperatures ($20^{\circ}C$, $40^{\circ}C$, and $60^{\circ}C$) and time (10 min, 20 min, and 30 min) using standardized soiled fabrics, i.e., 100% cotton, polyester/cotton (65%/35%), and 100% polyester woven fabric soiled with pigment/sebum, carbon black/mineral oil, soot/mineral oil, cocoa, blood, and red wine. Detergency at the washing condition of $20^{\circ}C$ and 30 min was higher than that at $40^{\circ}C$ and 10 min. In addition, detergency at the condition of $40^{\circ}C$ and 30 min was also higher than that at $60^{\circ}C$ and 10 minutes. This may be because a reduced washing effect at low washing temperatures was complemented by increased mechanical action over a long time. Further, washing temperature and time, with the same detergency, differed based on the type of fiber and soil. Also, the influence of a detergent on the detergency depends on the type of soil. The results suggest that energy and detergent have been consumed more than necessary in actual laundry. According to each type of fiber and soil, washing conditions designed to reduce the energy consumption of the washing process while maintaining the same detergency, were determined.

• Journal of Pharmaceutical Investigation
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• 제34권6호
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• pp.499-504
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• 2004

Paroxetine, a potent and selective serotonine reuptake inhibitor, has been used for the treatment of depression, obsessive-compulsive disorder, panic disorder and social phobia. The bioequivalence of two paroxetine preparations was evaluated according to the guidelines of Korea Food & Drug Administration (KFDA). The test product was Samchully Paroxetine $tablet^{\circledR}$ made by Samchully Pharm. Co. and the reference product was Seroxat $tablet^{\circledR}$ made by GlaxoSmithKline. Twenty healthy male subjects, $22.4{\pm}2.6$ years old and $63.8{\pm}4.2\;kg$, were divided into two groups and a randomized $2{\times}2$ cross-over study was employed. After one tablet containing 20 mg paroxetine was orally administered, blood was taken at predetermined time intervals and the concentration of paroxetine in plasma was determined using a validated HPLC method with fluorescence detector. Two pharmacokinetic parameters, $AUC_t$ and $C_{max}$, were calculated and analyzed statistically for the evaluation of bioequivalence of two products. Analysis of variance was carried out using logarithmically transformed parameter values. The 90% confidence intervals of $AUC_t$ and $C_{max}$ were log 0.84-log 1.16 and log 0.85-log 1.14, respectively. These values were within the acceptable bioequivalence intervals of log 0.8 to log 1.25. Thus, the criteria of the KFDA guidelines for the bioequivalence was satisfied, indicating that Samchully Paroxetine tablet is bioequivalent to Seroxat tablet.

• Animal Bioscience
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• 제34권1호
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• pp.85-92
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• 2021

Objective: The objectives of this study were to determine the in situ degradation parameters and appropriate feeding level of pineapple cannery by-products (PCB) based on the growth performance and blood parameters of growing Hanwoo (Bos taurus coreanae) steers fed various levels of PCB. Methods: Two ruminally cannulated Holstein cows were used for in situ disappearance rate measurements. Nylon bags (5×10 cm, 45 ㎛ pore size) filled with 5 g of PCB in triplicate were inserted into the ventral sac of the two cannulated Holsteins cows and incubated for 0, 2, 4, 8, 16, 24, and 48 h. A total of 16 castrated growing Hanwoo steers (12.5±0.5 months old, 302.9±25.7 kg of initial body weight [BW]) were used for the experiment. Animals were stratified by initial BW and then randomly assigned to one of four experimental diets (0%, 1.5%, 3.0%, or 6.0% of PCB, on the dry matter [DM] basis) fed for 91-d, including 30-d of adaptation. Results: Soluble fraction a of DM and crude protein (CP) was 61.9% and 86.0%, fraction b of DM and CP was 32.7% and 11.2%, and indigestible fraction c of DM and CP was 5.4% and 2.8%. The 6.0% PCB feeding group showed lower productivity compared to animals in the other treatments. Increasing the dietary level of PCB did not alter DM intake, but it was numerically lowest in the 6.0% feeding group. The gain to feed ratio was linearly decreased by increasing of PCB. The quadratic broken-line test estimated that 2.5% (DM basis) was the maximum feeding level of PCB in growing Hanwoo steers (y = 0.103 - 0.001×[1.245-x]2, R2 = 0.18). Conclusion: Diets containing up to 2.5% PCB can be fed to growing Hanwoo steers without adverse effects on growth performance.

• 대한통합의학회지
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• 제8권4호
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• pp.133-142
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• 2020

Purpose : The purpose of this study was to investigate the effective recovery method of exercise-induced fatigue and muscle pain by comparing the effect of the use of illite hot mat product and general hot mat product on the recovery of muscle pain induced by high intensity exercise. Methods : To measure and analyze the changes in lactic acid, CRP, and ACR according to the high-intensity circuit training program, this study was conducted for the healthy adult men and women, who exercise st the K-region sports center. A total of 45 subjects were studied in 15 groups of 15 patients who received an illite hot-water mat recovery group (A group), 15 general hot-water mat recovery group (B group), and 15 control group (C group). The circuit training exercise program was conducted as a one-time exercise, and each exercise time consisted of 30 minutes of warm-up exercise, 5 minutes of main exercise, 20 minutes of clean-up exercise, and 5 minutes of strength exercise. The intensity setting was high intensity of subjective exercise intensity It carried out by setting to (14-16RPE). Results : Changes in Lactic Acid Concentration There was a significant difference in the lactic acid concentrations between the groups after the high intensity circuit training program (p <.05). The illite rest group (A) decreased 7.71 mmol / L and the control group decreased 4.03 mmol / L. Significantly decreased (p <.05). Changes in ACR Concentration. There was a significant difference in the ACR concentrations Significant differences were found in CRP and ACR during the recovery period after exercise. (p <.05), the elite rest group (A) decreased 2.47 mg / mmol, and the control group increased 1.63 mg / mmol. There was a significant difference (p <.05). Conclusion: The static rest on a heated mat after high-intensity exercise has an effect on changes in blood lactate and ACR levels.

• 농업과학연구
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• 제44권4호
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• pp.574-585
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• 2017

This study was conducted to investigate effects of a potato byproduct on growth performance, blood metabolites, and carcass characteristics of Hanwoo steers. The palm oil coated potato byproduct was supplemented to the diet of Hanwoo steers to estimate the effect on growth performance, blood metabolites, and carcass characteristics during the late fattening period. Thirteen steers with initial body weight of control ($676.8{\pm}31.7kg$), treatment 1 ($671.8{\pm}46.2kg$) and treatment 2 ($672.8{\pm}31.1kg$) were used for 60 days, respectively. Average daily gain of steers in treatment 2 and control was greater than that in treastment 1 (p > 0.05). All steers in treatment 2 had a grade quality grading system than B in meat quantity and had a meat quality higher than the $1^{st}$ grade. According to the physicochemical analysis of longissimus muscle, treatment 2 had high brightness resulting from high meat quality (p > 0.05), and a decrease in redness and yellowness is seen as a dilution effect due to muscle hypertrophy (p > 0.05). The fatty acid composition showed low levels of linoleic acid (p = 0.039) and arachidonic acid (p = 0.008) in treatment 2. This resulted in lower polyunsaturated fatty acid (PUFA) levels (p = 0.034). On the other hand, high levels of oleic acid resulted in high levels of MUFA (p > 0.05). These results indicate that potato byproduct had similar effects with ${\beta}_2-adrenergic$ agonist (${\beta}_2-AA$). And there was no negative effect on the intramuscular fat. In conclusion, palm oil coated potato byproduct could be potentially used as an alternative growth enhancer.

• Journal of Chest Surgery
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• 제24권11호
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• pp.1058-1067
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• 1991

High potassium cardioplegia is a widely accepted procedure to enhance myocardial protection from ischemic injuries associated with open heart surgery. Maintaining optimum osmolarity of the cardioplegic solution is one of the required conditions for an ideal cardioplegic solution Albumin is an frequently added component for maintaining optimum osmolarity of clinically used cardioplegic solutions. But the source of albumin is human blood so that the supply is limited and the cost of manufacturing is relatively high. Recently there are moves to minimized the use of blood product for fear of blood-associated infections or immunological disorders. In this experiment, we substituted mannitol or glucose for albumin added to the cardioplegic solution which has been used at the Wonju Medical College, To determine whether addition of mannitol or glucose instead of albumin in the cardioplegic solution can produce satisfactory myocardial protection during ischemia, three different groups of isolated rat heart perfused by modified Langendorff technique were studied. Wonju Cardioplegic Solution was selected as a standard high potassium[18mEq/L of K+] cardioplegic solution. Three kinds of cardioplegic solution were made by modifying the composition maintaining the same osmolarity[339$\pm$1mOsm/Kg] Isolated rat heart were perfused initially with retrograde nonworking mode and then changed to working mode. After measuring the heart rate, systolic aortic pressure, aortic flow, coronary flow, ischemic arrest by aorta cross clamp and cardioplegia was made maintaining the temperature of water jacket at 10oC. The heart was rewarmed and reperfused after 60min of ischemic arrest with intermittent cardioplegia at the 30min interval. The time to return of heart beat and the time required to get. Regular heart beat were observed after reperfusion. The recovery rate of the functional variables-heart rate, systolic aortic pressure, aortic flow, coronary flow and cardiac output were calculated and compared among the three groups of different cardioplegia-albumin, mannitol, and glucose. The wet weight and dry weight was measured and the water content of the heart as figured out for comparison. The time to return of heart beat was fastest in the albumin group, The functional recovery rates were best in the albumin group also. In the above conditions, albumin was the best additive to the cardioplegic solution compared to the mannitol or glucose.

• 감성과학
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• 제13권2호
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• pp.309-316
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• 2010

본 연구에서는 단순 세정의 개념이 아닌 복합감각 증진 개념의 헬스케어 목욕 시스템을 개발하고자 한다. 헬스케어 감성욕조를 디자인하기 위해 우선 타입별 특성을 고찰하였다. 이를 기반으로 복합감각 자극용 전신 욕조를 개발하였고, 이 전신욕조는 감성자극용 전신욕조 월풀, 시청각 감각 자극 기능이 구비된 욕조 커버, 제어용 소형 PC, 터치패널, DMB 패널, 시각 감성 증진을 위한 칼라 가변형 LED 무드 시스템과 스피커로 구성되어 있다. 제작된 복합감각 증진 개념의 헬스케어 욕조시스템의 특성을 고찰하기 위해서 목욕을 하는 동안 인체 자율신경계에 미치는 영향을 분석하였다. 전반적으로 목욕 상태에서의 심박변이율은 실험 중 교감신경에 비해 부교감신경이 계속 증가하는 것으로 나타났고, 목욕을 시작한지 15분이 경과하면 교감신경이 증가하고 부교감신경이 감소하여, 피험자가 목욕을 한지 15분일 때 가장 편안한 상태를 느끼는 것을 알 수 있었다. 또한 목욕 실험 결과 전후의 혈압이 최고 16mmHg까지 떨어진 것으로 나타났으나 맥박은 증가하였다. 이것은 심장으로부터 혈액의 유출량이 많아져 혈류량이 증가하는 것으로 복합감각 증진용 헬스케어 욕조 시스템을 이용한 목욕이 혈액 순환에 긍정적인 영향을 보였다. 본 연구의 결과를 가지고 복합감각 기능증진 헬스케어 목욕시스템의 사용성과 사용자의 생리분석을 통한 기초결과를 통해서 사용자의 생리변화를 분석함으로써 제품의 사용성과 사용자의 생리적 변화 및 감성 간의 관계를 보다 더 명확하게 밝혀낼 수 있으리라 기대한다.

• Clinical and Experimental Reproductive Medicine
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• 제30권3호
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• pp.203-206
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• 2003

Objectives: Controversial arguments exists on both the case for and against on the accumulation of mitochondrial DNA (mtDNA) deletion in association to tissue and age. The debate continues as to whether this mutation is a major contributor to the phenotypic expression of aging and common degenerative diseases or simply a clinical insignificant epiphenomenon. The objective of this study was to determine whether the accumulation of mtDNA deletion is correlated with age-related and tissue-specific variation. Materials and Methods: One hundred and fifty-seven tissues from blood, ovary, uterine muscle, and abdominal muscle were obtained from patients ranging in age from 31$\sim$60 years. After reviewing the clinical reports, patients with mitochondrial disorder were excluded from this study. The tissues were obtained at gynecological surgeries with the consent of the patient. Total DNA isolated from blood, ovary, uterine muscle, and abdominal muscle was amplified by two rounds of PCR using two pairs of primers corresponding to positions 8225-8247 (sense), 13551-13574 (antisense) for the area around deleted mtDNA and 8421-8440 (sense), 13520-13501 (antisense) for nested PCR product. A statistical analysis was performed by $x^2$-test. Results: About 0% of blood, 94.8% of ovary, 71.4% of uterine muscle, and 86.1% abdominal muscle harbored mtDNA deletion. When we examined the proportion of deleted mtDNA according to age deletion rate was 90% of ovary, 63.6% of uterine muscle, 77.7% of abdominal muscle in thirties and 100% of all tissue in fifties. Conclusion: The findings of this study suggest that the mtDNA deletion is varied in tissue-specific pattern and increases with aging.

• 대한의생명과학회지
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• 제12권4호
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• pp.375-383
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• 2006

The physiological responses following stress are different in individual or personality. We performed this study to clarify gender differences in influences of noise stress on physiological factors. 70 healthy subjects, which was divided man (n=30) and woman (n=40) groups, were exposed to 85 decibels of excavator noise for 15 minutes. Cardiac factors such as heart rate (HR), systolic and diastolic blood pressures (SBP and DBP, respectively), and heart rate-systolic pressure product (RPP) were determined. Transcranial Doppler ultrasound (TCD) was used to measure mean blood flow velocity (Vm), pulsatility index (PI), and resistance index (RI) in the middle, anterior and posterior cerebral arteries (MCA, ACA and PCA, respectively) before and during noise exposure. Cortisol level and hematological variables were also measured before (baseline) and immediately after the end of noise exposure. In the both groups HR, SBP, and RPP significantly decreased during noise exposure (P<0.05) but not significantly different between two groups (P>0.05). Vms of three cerebral arteries in man group decreased, whereas Vm of PCA in woman group fell during noise exposure (P<0.05). Vm, PI and RI in MCA and ACA during noise exposure were low in man group compared with woman group (P<0.05). Vm of PCA was low, whereas PI and RI of PCA were high in man group compared with woman group during noise exposure (P<0.05). Total leukocyte and red blood cell (RBC) counts slightly decreased during noise exposure but not significant (P>0.05). Levels in hematological variables decreased but not significant changed following noise exposure. Decreased rate of total leukocyte in man group was higher (P<0.05). Cortisol levels in the both groups decreased immediately after the end of noise exposure, while the decreased rate in man group was greater than that in woman group (p<0.05). These findings indicate that a transient exposure to experimental excavator noise may cause decreased changes in cardiac factors, cerebral hemodynamics and cortisol levels and the changes may be greater in men than in women.