• 한국자동차공학회논문집
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• 제5권2호
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• pp.50-59
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• 1997

When a front head of train enters a tunnel at a high speed, compression wave is generated at tunnel entrance due to the confinement effect and propagated along the tunnel with sound of speed. The propagated compression wave is reflected at tunnel exit due to abrupt pressure change at passage. The reflected wave is expansion pressure wave. And when the rear head of train goes through the tunnel entrance, another expansion pressure wave is generated and propagated along the tunnel. The pressure drop occurs seriously around train when the two expansion pressure waves come cross on train in the tunnel. In order to reduce the pressure drop, the compression wave front must be controlled because the intensity and magnitude of pressure drop is nearly proportional to that of compression wave at tunnel entrance. This study relates to reduction of the pressure wave gradient with respect to tunnel entrance shape change with various kind of angle and rounding. The results show characteristics of wave propagation in tunnel, usefulness of characteristic curve to estimate proper time domain size in numerical study and measuring time in actual experiment. Also rounding is contributed to improve pressure wave front even if its radius is very small at tunnel entrance. In order to improve of pressure wave front at tunnel entrance, proper angle is prefered to rounding with big radius and an angle of around 14$^{\circ}$ is recommended according to this simulations, And it is expected to reduce additional pressure drop in tunnel when the location and the size of the internal space for attendant equipment are considered in advance.

• Animal cells and systems
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• 제4권3호
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• pp.279-285
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• 2000

Vitamin E-succinate (VES) treatment of U937 human monoblasts induced cells to undergo apoptosis. After 96 h of VES treatment at 10 $\mu$/ml, more than 80% of cells appeared apoptotic. Evidence for apoptosis by VES was based on propidium iodide staining for detection of chromatin condensational fragmentation and electrophoretic DNA ladder formation. Western blot analyses showed a transient increase in Fas and p21 protein levels up to 48 h alter the VES treatment. Protein expression and activity of CDK1 and lamin B degradation were remarkably induced by VES, following the cleavage of caspase-3 after 48 h. The VES-induced apoptosis was found to involve activation of PKC as shown by increases in membrane translocation of PKC$\beat$II and PKC activity. Pretreatment of GF109203X (PKC inhibitor) prior to VES treatment almost completely inhibited the induction of apoptosis as assessed by blockage of VES-induced caspase-3 activity and DNA fragmentation. However, GF109203X h8d no effect on the VES-induced nitric oxide synthesis, which was required for monocvtic differentiation in our previous report (J Cell Sci 111, 435, 1998). Taken together, our data suggest that induction of apoptosis by VES in U937 cells occurs through activation of PKC-$\beat$II resulting in the activation of caspase-3 cascade and is independent of nitric oxide.

• 한국미생물·생명공학회지
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• 제13권2호
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• pp.119-127
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• 1985

여러 가지 Escherchia coli 변이주의, glucose 와 ammonium염과 같은 간단한 기질로부터 방향족 아미노산 특히 phenylanine을 생합성하는 능력을 비교 검토한 결과 방향족 아미노산 생합성과정중 common pathway의 첫 번째 반응이 phenylanine 생합성에 가장 큰 영향을 준다는 것을 확인하였다. 따라서 관계효소인 DAHP synthase의 효소활성과 생합성에 관련된 각종 대사 제어작용을 효과적으로 제거시킴으로서 phenylalanine 생산량을 크게 높일 수 있었으며 더욱이 phenylalanine terminal pathway의 첫 단계 반응을 촉매하는 prephenate de-hydratase의 효소활성과 효소생합성에 관련된 제어 작용도 동시에 제거하면 phenylalanine생산이 상승적으로 증가됨을 보였다. 한편 방향족아미노산의 transport system에 관계하는 arop유전자의 변이는 phenylalanine생산을 크게 저하시키는 효과를 나타내었다.

• 동아시아식생활학회지
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• 제24권3호
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• pp.359-367
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• 2014

This study analyzed phytochemicals, including various carotenoids, tocopherol and L-ascorbic acid, in green, yellow and orange paprikas (GP, YP and OP) and measured the preventive effects of lipophilic extracts from different colored paprikas on the blockage of gap junctional intercellular communication (GJIC), which is known as a cellular event associated with tumor promotion. Main carotenoids were lutein and ${\beta}$-carotene in GP, lutein, ${\beta}$-carotene, capsanthin, violaxanthin, ${\beta}$-carotene and capsorubin in YP, and lutein, ${\beta}$-carotene, cryptoxanthin and zeaxanthin in OP. Total carotenoid contents were $65.54{\pm}15.87$ mg/100 g dw in OP, $11.98{\pm}0.69$ mg/100 g dw in YP and $10.30{\pm}1.43$ mg/100 g dw in GP. Tocopherol contents were highest in GP compared with in YP and OP, whereas L-ascorbic acid contents were very high in all paprikas. We determined the non-cytotoxic levels of paprika extracts by MTT assay, which showed less formation of reactive oxygen species (ROS) induced by $500{\mu}M$ $H_2O_2$ for 1h. Finally, we showed that pretreatment of paprika extracts prevented inhibition of GJIC induced by $500{\mu}M$ $H_2O_2$ by the scrape-loading/dye-transfer technique. In conclusion, each colored paprika has unique phytochemicals and showed a protective effect on inhibition of GJIC.

• Wind and Structures
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• 제25권3호
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• pp.233-259
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• 2017

Investigations on simulated near-surface atmospheric boundary layer (ABL) in an open-jet facility are carried out by conducting experimental tests on small-scale models of low-rise buildings. The objectives of the current study are: (1) to determine the optimal location of test buildings from the exit of the open-jet facility, and (2) to investigate the scale effect on the aerodynamic pressure characteristics. Based on the results, the newly built open-jet facility is well capable of producing mean wind speed and turbulence profiles representing open-terrain conditions. The results show that the proximity of the test model to the open-jet governs the length of the separation bubble as well as the peak roof pressures. However, test models placed at a horizontal distance of 2.5H (H is height of the wind field) from the exit of the open-jet, with a width that is half the width of the wind field and a length of 1H, have consistent mean and peak pressure coefficients when compared with available results from wind tunnel testing. In addition, testing models with as large as 16% blockage ratio is feasible within the open-jet facility. This reveals the importance of open-jet facilities as a robust tool to alleviate the scale restrictions involved in physical investigations of flow pattern around civil engineering structures. The results and findings of this study are useful for putting forward recommendations and guidelines for testing protocols at open-jet facilities, eventually helping the progress of enhanced standard provisions on the design of low-rise buildings for wind.

• Wind and Structures
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• 제22권2호
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• pp.253-272
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• 2016

The paper concerns with the method and results of wind tunnel investigations of the Strouhal number (St) of a stationary iced cable model of cable-supported bridges with respect to different angles of wind attack. The investigations were conducted in the Climatic Wind Tunnel Laboratory of the Czech Academy of Sciences in $Tel{\check{c}}$. The methodology leading to the experimental icing of the inclined cable model was prepared in a climatic section of the laboratory. The shape of the ice on the cable was registered by a photogrammetry method. A section of an iced cable model with a smaller scale was reproduced with a 3D printing procedure for subsequent aerodynamic investigations. The St values were determined within the range of the Reynolds number (Re) between $2.4{\cdot}10^4$ and $16.5{\cdot}10^4$, based on the dominant vortex shedding frequencies measured in the wake of the model. The model was oriented at three principal angles of wind attack for each of selected Re values. The flow regimes were distinguished for each model configuration. In order to recognize the tunnel blockage effect the St of a circular smooth cylinder was also tested. Good agreement with the reported values in the subcritical Re range of a circular cylinder was obtained. The knowledge of the flow regimes of the airflow around an iced cable and the associated St values could constitute a basis to formulate a mathematical description of the vortex-induced force acting on the iced cable of a cable-supported bridge and could allow predicting the cable response due to the vortex excitation phenomenon.

• 한국항공우주학회지
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• 제40권6호
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• pp.485-491
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• 2012

저 레이놀즈 수 조건에서 폐쇄형 풍동 시험부에 설치된 에어포일 주위의 벽면효과에 대한 실험적 연구를 수행하였다. 이를 위해 풍동시험이 수행되었으며, 시험은 자유흐름속도 10 ~ 31 m/s와 시위기준 레이놀즈 수 $1.5{\times}10^5{\sim}4.6{\times}10^5$ 조건에서 수행되었다. 시위대 시험부 폭 비인 기준폐쇄율은 27.8%, 38.5%, 41.7%, 45.5% 그리고 55.6%이다. 시험 결과 벽면효과에 의해 에어포일 표면에서의 천이점이 후방으로 미미하게 이동함을 확인하였다. 또한, 벽면압력을 이용한 벽면효과 보정을 위해서는 에어포일을 중심으로 시위의 세배 이상의 벽면압력이 필요함을 확인하였다.

• Biomolecules & Therapeutics
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• 제20권3호
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• pp.306-312
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• 2012

Resveratrol (trans-3,5,4'-trihydroxystilbene) has received considerable attention recently for the potential neuroprotective effects in neurodegenerative disorders where heme oxygenase-1 (HO-1) and sirtuin 1 (SIRT1) represent promising therapeutic targets. Resveratrol has been known to increase HO-1 expression and SIRT1 activity. In this study, the effects of resveratrol and trans-3,5,4'-trimethoxystilbene (TMS), a resveratrol derivative, on cytotoxicity caused by glutamate-induced oxidative stress, HO-1 expression, and SIRT1 activation have been investigated by using murine hippocampal HT22 cells, which have been widely used as an in vitro model for investigating glutamate-induced neurotoxicity. Resveratrol protected HT22 neuronal cells from glutamate-induced cytotoxicity and increased HO-1 expression as well as SIRT1 activity in a concentration-dependent manner. Cytoprotection afforded by resveratrol was partially reversed by the specific inhibition of HO-1 expression by HO-1 small interfering RNA and the nonspecific blockage of HO-1 activity by tin protoporphyrin IX, but not by SIRT1 inhibitors. Surprisingly, TMS, a resveratrol derivative with methoxyl groups in lieu of the hydroxyl groups, and trans-stilbene, a non-hydroxylated analog, failed to protect HT22 cells from glutamate-induced cytotoxicity and to increase HO-1 expression and SIRT1 activity. Taken together, our findings suggest that the cytoprotective effect of resveratrol was at least in part associated with HO-1 expression but not with SIRT1 activation and, importantly, that the presence of hydroxyl groups on the benzene rings of resveratrol appears to be necessary for cytoprotection against glutamate-induced oxidative stress, HO-1 expression, and SIRT1 activation in HT22 neuronal cells.

• Journal of Audiology & Otology
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• 제24권1호
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• pp.10-16
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• 2020

Background and Objectives:The blockage of adenosine receptors by caffeine changes the levels of neurotransmitters. These receptors are present in all parts of the body, including the auditory and vestibular systems. This study aimed to evaluate the effect of caffeine on evoked potentials using auditory brainstem responses (ABRs) and cervical vestibular-evoked myogenic potentials (cVEMPs) in a double-blind placebo-controlled study. Subjects and Methods: Forty individuals (20 females and 20 males; aged 18-25 years) were randomly assigned to two groups: the test group (consuming 3 mg/kg pure caffeine powder with little sugar and dry milk in 100 mL of water), and the placebo group (consuming only sugar and dry milk in 100 mL water as placebo). The cVEMPs and ABRs were recorded before and after caffeine or placebo intake. Results: A significant difference was observed in the absolute latencies of I and III (p<0.010), and V (p<0.001) and in the inter-peak latencies of III-V and I-V (p<0.001) of ABRs wave. In contrast, no significant difference was found in cVEMP parameters (P13 and N23 latency, threshold, P13-N23 amplitude, and amplitude ratio). The mean amplitudes of P13-N23 showed an increase after caffeine ingestion. However, this was not significant compared with the placebo group (p>0.050). Conclusions: It seems that the extent of caffeine's effects varies for differently evoked potentials. Latency reduction in ABRs indicates that caffeine improves transmission in the central brain auditory pathways. However, different effects of caffeine on auditory- and vestibular-evoked potentials could be attributed to the differences in sensitivities of the ABR and cVEMP tests.

• 대한청각학회지
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• 제24권1호
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• pp.10-16
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• 2020

Background and Objectives:The blockage of adenosine receptors by caffeine changes the levels of neurotransmitters. These receptors are present in all parts of the body, including the auditory and vestibular systems. This study aimed to evaluate the effect of caffeine on evoked potentials using auditory brainstem responses (ABRs) and cervical vestibular-evoked myogenic potentials (cVEMPs) in a double-blind placebo-controlled study. Subjects and Methods: Forty individuals (20 females and 20 males; aged 18-25 years) were randomly assigned to two groups: the test group (consuming 3 mg/kg pure caffeine powder with little sugar and dry milk in 100 mL of water), and the placebo group (consuming only sugar and dry milk in 100 mL water as placebo). The cVEMPs and ABRs were recorded before and after caffeine or placebo intake. Results: A significant difference was observed in the absolute latencies of I and III (p<0.010), and V (p<0.001) and in the inter-peak latencies of III-V and I-V (p<0.001) of ABRs wave. In contrast, no significant difference was found in cVEMP parameters (P13 and N23 latency, threshold, P13-N23 amplitude, and amplitude ratio). The mean amplitudes of P13-N23 showed an increase after caffeine ingestion. However, this was not significant compared with the placebo group (p>0.050). Conclusions: It seems that the extent of caffeine's effects varies for differently evoked potentials. Latency reduction in ABRs indicates that caffeine improves transmission in the central brain auditory pathways. However, different effects of caffeine on auditory- and vestibular-evoked potentials could be attributed to the differences in sensitivities of the ABR and cVEMP tests.