• Journal of the Korean Magnetic Resonance Society
• /
• v.17 no.1
• /
• pp.59-66
• /
• 2013

BldD, a developmental transcription factor from Streptomyces coelicolor, is a homodimeric, DNA-binding protein with 167 amino acids in each subunit. Each monomer consists of two structurally distinct domains, the N-terminal domain (BldD-NTD) responsible for DNA-binding and dimerization and the C-terminal domain (BldD-CTD). In contrast to the BldD-NTD, of which crystal structure has been solved, the BldD-CTD has been characterized neither in structure nor in function. Thus, in terms of structural genomics, structural study of the BldD-CTD has been conducted in solution, and in the present work, mainchain NMR assignments of the recombinant BldD-CTD (residues 80-167 of BldD) could be achieved by a series of heteronuclear multidimensional NMR experiments on a [$^{13}C/^{15}N$]-enriched protein sample. Finally, the secondary structure prediction by CSI and TALOS+ analysis using the assigned chemical shifts data identified a ${\beta}-{\alpha}-{\alpha}-{\beta}-{\alpha}-{\alpha}-{\alpha}$ topology of the domain. The results will provide the most fundamental data for more detailed approach to the atomic structure of the BldD-CTD, which would be essential for entire understanding of the molecular function of BldD.

• Proceedings of the Korean Biophysical Society Conference
• /
• 2003.06a
• /
• pp.49-49
• /
• 2003

BldD is one of a set of morphological differentiation regulators in Streptomyces coelicolor. The bldD coding region was cloned and heterologously expressed in Escherichia coli. Previous analysis showed the splitting appearance of purified BldD.

• Journal of radiological science and technology
• /
• v.35 no.3
• /
• pp.249-254
• /
• 2012

The aim of this study was to investigate the setup accuracy for tangential breast treatment patients using electronic portal image and 2-D reconstruction image Twenty two patients undergoing tangential breast treatment. To explore the setup accuracy, distances between chosen landmarks were taken as reference parameters. The difference between measured reference parameters on simulation films and electronic portal images (EPIs) was calculated as the setup error. A total of 22 simulation films and 110 EPIs were evaluated. In the tangential fields, the calculated reference parameters were the central lung distance (CLD), central soft-tissue distance (CSTD), and above lung distance (ALD), below lung distance (BLD). In the medial tangential field, the average difference values for these parameters were 1.0, -6.4, -2.1 and 2.0, respectively; and the ${\sigma}$ values were 1.5, 2.3, 4.1 and 1.1, respectively. In the lateral tangential field, the average difference values for these parameters were -1.5, -4.3, -2.7 and -1.3, respectively; and the ${\sigma}$ values were 3.3, 2.1, 2.9 and 2.5, respectively. CLD, CSTD, ALD and BLD in the tangential fields are easily identifiable and are helpful for detecting setup errors using EPIs in patients undergoing tangential breast radiotherapy treatment.

• Journal of Oral Medicine and Pain
• /
• v.11 no.1
• /
• pp.57-66
• /
• 1986

A study of occlusal relationship and TMJ dysfunction, parafunctional relationship and TMJ dysfunction using PRI was investigated in 23 dental students without history of being treated, ranging from 19 to 24 years old. The author used Denar Pantronic and D5-A articulator to study above. The obtained results were as follows : 1. Distribution of PRI in experimental subjects was as follows. None 13%, Slight 43%, Moderate 40%, Severe 4% Prevalance of TMJ Dysfunction was 87% 2. PRI scores of occlusal group Aoi (less than 5 in Helkimo occlusion/articulation index) was $11.72\pm2.32$ , those of occlusal group Boi (more than 5 in Helkimo occlusion/articulation index) 23.0 $\pm$ 8.06. There was a significant difference between group Aoi and group Boi(P<0.01) 3. PRI scores of occlusal group Ald (no eccentric interference, less than 0.5mm in lateral shift of slide in centric) was 12.0 $\pm$ 2.49, those of occlusal group Bld(no eccentric interference, less than 0.5mm in lateral shift of slide in centric) 24.5$\pm$5.33 There was a significant difference between group Ald and group Bld(P<0.05) 4. Prevalance of parafunction subjects(%) was 65.2%. 5. PRI scores of parafunction group was 21.6 $\pm$ 9.48, those of nonparafunctional group 18.7 $\pm$ 9.06. There was not significant difference between parafunctional group and nonparafunctional group(P>0.1)

• Macromolecular Research
• /
• v.11 no.2
• /
• pp.80-86
• /
• 2003

The polymerization of a cyclopolymerizable disubstituted dipropargyl ether, his(3-trimethylsilyl-2-propynyl)ether (BTPE), was attempted by various transition metal catalysts. The yield for the polymerization of BTPE was generally low, which is possibly due to the steric hindrance of bulky substituents. In general, the catalytic activities of Mo-based catalysts were found to be greater than those of W-based catalysts. The highest yield was obtained when the MoCl$_{5}$,-EtAlCl$_2$(1:2) catalyst system was used. The copolymerization of BTPE and diethyl dipropargylmalonate yielded a random copolymer with conjugated polymer backbone. However the polymers were partially desilylated, depending on the reaction conditions. The thermal and morphological properties of the resulting polymers were also discussed.d.

• Asian-Australasian Journal of Animal Sciences
• /
• v.17 no.12
• /
• pp.1725-1728
• /
• 2004

An experiment was conducted to investigate the efficiency of transfer of dietary iron sources to eggs of laying hens. Eighty ISA-Brown laying birds of 30 wk old were housed in 40 cages of 2 birds each. Eight birds in four cages were assigned to one of the following ten treatments: T1; control, T2; 100 ppm iron supplementation with iron-methionine chelate (Fe-Met-100), T3; Fe-Met- 200, T4; Fe-Met-300, T5; 100 ppm iron supplementation with iron sulfate ($FeSO_4$-100), T6; $FeSO_4$-200, T7; $FeSO_4$-300, T8; 100 ppm iron supplementation with Availa-$Fe^{(R)}$ (Availa-Fe-100), T9; Availa-Fe-200 and T10; Availa-Fe-300. Results of 40 d feeding trial showed that there were no consistent responses in laying performance by source and level of iron supplementation. However, eggshell strength and color were improved by Fe supplementation. Egg iron content was maximized at 10-15 days after feeding supplemental Fe. Fe- Met was the most effective source in enriching Fe of eggs followed by Availa-Fe and $FeSO_4$. Increasing supplementary Fe level more than 100 ppm was not effective in Fe-Met and Availa-Fe treatments. Average Fe enrichment of 18% was achieved after feeding Fe-Met-100 for 15 d. In conclusion, enrichment of Fe in egg could be effectively achieved by supplementation of Fe-Met-100 for 15 d.