• 국제강구조저널
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• 제18권5호
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• pp.1541-1559
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• 2018

This paper addresses the dynamic loading characteristics of the shock tube onto sandwich steel beams as an efficient and accurate alternative to time consuming and complicated fluid structure interaction using finite element modeling. The corrugated sandwich steel beam consists of top and bottom flat substrates of steel 1018 and corrugated cores of steel 1008. The corrugated core layers are arranged with non-uniform thicknesses thus making sandwich beam graded. This sandwich beam is analogous to a steel beam with web and flanges. Substrates correspond to flanges and cores to web. The stress-strain relations of steel 1018 at high strain rates are measured using the split-Hopkinson pressure. Both carbon steels are assumed to follow bilinear strain hardening and strain rate-dependence. The present finite element modeling procedure with an improved dynamic impulse loading assumption is validated with a set of shock tube experiments, and it provides excellent correlation based on Russell error estimation with the test results. Four corrugated graded steel core arrangements are taken into account for core design parameters in order to maximize mitigation of blast load effects onto the structure. In addition, numerical study of four corrugated steel core placed in a reverse order is done using the validated finite element model. The dynamic behavior of the reversed steel core arrangement is compared with the normal core arrangement for deflections, contact force between support and specimen and plastic energy absorption.

• 한국식물병리학회지
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• 제14권5호
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• pp.413-417
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• 1998

Magnaporthe grisea, the casual agent of rice blast, requires formation of an appressorium, a dome-shaped and well melanized infection structure, to penetrate its host. Environmental cues that induce appressorium formation include hydrophobicity and hardness of contact surface and chemicals from its host. Artificial surfaces are widely used to induce appressorium formation, but frequencies of appressorium induction are not always consistent. To understand variable induction of appressorium formation in M. grisea, several factors were tested on GelBond. High levels of appressorium formation were induced over a wide range of temperature (20~3$0^{\circ}C$) and pH (4~7). spore age up to 3-week-old did not significantly affect appressorium formation, but only a few apressoria on GelBond. However, adenosine specifically inhibited appressorium formation. Adenosine inhibition of appressorium formation was restored by exogenous addition of cAMP. Germ tube tips of M. grisea maintained the ability to differentiate appressoria by chemical inducers on GelBond at least up to 16 h after conidia germination. These results suggest that environmental factors have little effect on the variable induction of appressorium formation on the artificial surface in M. grisea.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2014년도 추계학술대회 및 정기총회
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• pp.43-43
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• 2014

The rice blast disease caused by of Magnaporthe oryzae is one of the most destructive diseases of rice. By the microarray analysis, we profiled expression changes of genes during conidiation and found out many putative genes that are up-regulated. Among those, we first selected MGG_06399 encoding a dual-specificity tyrosine-regulated protein kinase (DYRK), homologous to YAK1 in yeast. To investigate functional roles of MoYAK1, We made ${\Delta}Moyak1$ mutants by homology dependent gene replacement. The deletion mutant showed a remarkable reduction in conidiation and produced abnormally shaped conidia smaller than those of wild type. The conidia form ${\Delta}Moyak1$ were able to develop a germ tube, but failed to form apppressoria on a hydrophobic coverslip. The ${\Delta}Moyak1$ formed appressria on a hydrophobic cover slip when exogenous cAMP was induced, but the appressoria shape was abnormal. The ${\Delta}Moyak1$ also formed appressoria abberent in shape on onion epidermis and rice sheaths and failed to penetrate the surface of the plants. These data indicate that MoYAK1 is associated with cAMP/PKA pathway and important for conidiation, appressorial formation and pathogenic development in Magnaporthe oryzae. Detailed characterization of MoYAK1 will be presented.

• 한국응용곤충학회지
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• 제5_6권
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• pp.19-26
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• 1968

휴지상태에 있는 성숙한 도열병균 포자 1,000개를 관찰한 결과 각 세포에 단핵을 가지는 포자비율이 $95\%$로 단연 많았고 또 미숙회자인 1세포 1핵 포자로부터 출발하여 3세포 3핵인 성숙포자가 형성됨을 관찰할 수 있었다. 분열 중인 핵은 배열된 염색체로서 관찰할 수 있었으며, 염색체수는 n=3,4,5,6개로 나타났으나, n=5개가 가장 많았다. 핵이 발아관으로 이동하는 방법은 첫째로 휴지상태에 있던 핵이 분열하여 2핵이 되고 그 중 1핵이 발아관에 이동하고 1핵은 세포내에 머무는 것과$(43\%)$, 둘째 방법은 핵분열 없이 직접 세포내의 1핵이 발아관을 따라 이동하는 것들이 $(57\%)$ 있다. 또 부착기내의 핵을 염색하여 관찰한 결과 무핵의 부착기가 상당히 많았으나 핵질을 가진 500개의 부착기 중에 단핵이 476개로서 $95.2\%$였다(Chromosome 으로 관찰된 4부착기는 제외). 이상의 결과로서 도열병균은 전생활환을 통하여 단핵을 함유한다는 것을 인정할 수 있으며, 도열병균포자가 다세포일지라도 Homokaryon이라고 생각되므로 단포자분리균을 도열병에 관한 모든 시험에 공시하는 것은 합당하다고 할 수 있다.

• 한국추진공학회지
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• 제11권2호
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• pp.26-36
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• 2007

슬랏이 있는 튜브형 그레인 형상의 알루미늄 함유 PCP계 또는 HTPB계 추진제를 충전한 고체 추진기관에 대해 노즐 내열재의 열반응 특성을 분석하였다. 노즐 내열재에서 채취한 산화알루미늄 입자의 SEM 사진을 통해 상대적으로 크기가 작고 저 함량의 산화제를 포함한 PCP계 추진제는 HTPB계 추진제보다 알루미늄 분말들이 응집될 가능성이 크다는 것을 확인할 수 있었다. PCP계 추진제를 적용한 경우에는 그레인 슬랏과 일치하는 노즐 축소부 내열재의 원주방향 4개 영역에서 큰 입자의 산화알루미늄 충돌로 인해 국부적으로 삭마가 많았지만 HTPB계 추진제는 연소가스내 $H_2O$ 및 $CO_2$의 몰분율이 상대적으로 많음으로 인해 노즐 토출관, 목삽입재 및 확대부 내열재에서 화학반응으로 인한 삭마가 많았다.

• 한국식물병리학회지
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• 제3권2호
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• pp.100-107
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• 1987

동일한 벼 품종은 수분함량이 다른 토양에서 재배했을 경우, 도열병에 대한 감수성에 차이가 생기는데 그 원인을 조사하기 위하여 잎 표피에서 분생포자발아 및 부착기형성까지의 도열병균 침입전 행동을 광학현미경과 주사전자현미경(SEM)으로 관찰하였다. 수분함량이 다른 토양에서 자란 벼 잎 사이에 표피세포의 외부형태, 잎 표피에서의 분생포자발아관의 생장 및 생장방향, 부착기의 형태 및 크기는 차이가 없었다. 부착기는 토양수분처리와 상관없이 기동세포$(35\~48\%)$위에 가　 많이 형성되었고 단세포$(19\~27\%)$, 장세포 및 공변세포$(13\~20\%)$의 순이었다. 모분상의 부착기형성은 관찰되지 않았다. 본 시험결과, 수분함량이 다른 토양에서 자란 벼 사이에 나타나는 도열병에 대한 감수성의 차이는 도열병균의 침입전 행동에서 기인하는 것이 아닌 것으로 생각된다.

• Structural Engineering and Mechanics
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• 제32권2호
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• pp.193-212
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• 2009

The resilience of a city confronted with a terrorist bomb attack is the background of the paper. The resilience strongly depends on vital infrastructure and the physical protection of people. The protection buildings provide in case of an external explosion is one of the important elements in safety assessment. Besides the aspect of protection, buildings facilitate and enable many functions, e.g., offices, data storage, -handling and -transfer, energy supply, banks, shopping malls etc. When a building is damaged, the loss of functions is directly related to the location, amount of damage and the damage level. At TNO Defence, Security and Safety methods are developed to quantify the resilience of city infrastructure systems (Weerheijm et al. 2007b). In this framework, the dynamic response, damage levels and residual bearing capacity of multi-storey RC buildings is studied. The current paper addresses the aspects of dynamic response and progressive collapse, as well as the proposed method to relate the structural damage to a volume-damage parameter, which can be linked to the loss of functionality. After a general introduction to the research programme and progressive collapse, the study of the dynamic response and damage due to blast loading for a single RC element is described. Shock tube experiments on plates are used as a reference to study the possibilities of engineering methods and an explicit finite element code to quantify the response and residual bearing capacity. Next the dynamic response and progressive collapse of a multi storey RC building is studied numerically, using a number of models. Conclusions are drawn on the ability to predict initial blast damage and progressive collapse. Finally the link between the structural damage of a building and its loss of functionality is described, which is essential input for the envisaged method to quantify the resilience of city infrastructure.

• 한국건축시공학회지
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• 제15권3호
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• pp.273-280
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• 2015

본 연구는 3시간 내화성능을 만족할 수 있는 고성능 무내화피복 CFT 기둥 개발을 위한 연구로서, 고로슬래그 미분말 치환율에 따른 성능평가를 실시한 결과 고로슬래그 미분말 치환율이 증가함에 따라 내화성능이 개선되는 것을 확인 할 수 있었으며, 내화성능을 고려한 CFT 기둥용 100MPa 충전콘크리트의 고로슬래그 미분말 적정 사용량은 40%로 나타났다. 이에 따라 고로슬래그 미분말 40% 혼입 100MPa 충전콘크리트를 적용한 CFT 실물 시험체를 대상으로 재하하중별 내화시험을 실시한 결과 재하하중이 증가함에 따라 내화성능 발현시간이 감소하는 것으로 나타났으며, 재하하중 2,000kN에서는 240분 이상의 내화성능을 나타내었고, 3,000kN에서는 184분, 4,000kN에서는 120분의 내화성능을 나타내었다.

• 한국추진공학회지
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• 제18권1호
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• pp.8-15
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• 2014

고체 로켓 추진기관의 노즐목 삽입재에 적용하는 그라파이트의 삭마율 특성을 분석하였다. 지상연소시험은 3종류의 일반적인 노즐형태를 갖는 추진기관을 사용하여 수행하였다. 즉, De-Laval 형태, 토출관 형태, 내삽 형태이다. 13종류의 서로 다른 형상의 추진기관에 다양한 추진제를 적용하였고, 노즐목 위치에 그라파이트를 적용하여 총 37회의 연소시험을 수행하였다. 분석결과 그라파이트의 삭마율은 연소실 평균압력이 상승함에 따라, 산화몰분율이 증가함에 따라 증가함을 알 수 있다.

• Asian-Australasian Journal of Animal Sciences
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• 제19권9호
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• pp.1229-1233
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• 2006

To find the candidate genes concerned with ovulation rate of sheep, Differential Display Reverse Transcription Polymerase Chain Reaction was employed to find the differently expressed cDNA controlling ovulation in the Small Tail Han sheep of polyembryony and in Tan sheep of single birth. Twenty-four primer pairs of three anchored primers and eight arbitrary primers were assembled to amplify the specialized bands from these sheep. Positive cross tests were applied to optimize the ascertainable PCR conditions in which different special bands can be identified by silver strain in one PCR tube. After eliminating the false positive PCR products by Northern hybridization, 24 differential display bands were acquired from the ovary in the Small Tail Han sheep. These EST bands were sequenced and 18 different ESTs were found in which five ESTs had several copies and 13 ESTs had only one copy. Comparing these ESTs with homologous sequences by BLAST in the GenBank, there were six ESTs with known open reading frame (ORF) and function, three ESTs with known ORF and no function, and 9 ESTs without homologous sequence. These ESTs partly represent several genes such as NOS2, tensin, TCRA, CDKN1A, ESR1 and ACTB which express especially in Small Tail Han sheep.