• 한국어병학회지
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• 제25권3호
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• pp.231-241
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• 2012

2007년~2011년 하절기에 국내 동 서 남해안과 제주의 육상양식장, 가두리 및 축제식 양식장에서 사육중인 넙치 (Paralichthys olivaceus), 조피볼락 (Sebastes schlegeli), 참돔 (Pagrus major), 흰다리새우 (Litopenaeus vannamei) 의 총 2,413마리에 대하여 세균과 기생충의 감염현황을 조사하였다. 조사기간(2007년~2011년) 사이 어종별 병원체에 대한 감염양상을 종합하면, 기생충과 세균의 단독감염이 혼합 감염에 비하여 비교적 높게 나타났으며, 단독감염 가운데 기생충이 세균보다 감염률이 높은 경향을 나타내었다. 조사기간 동안 넙치, 조피볼락, 새우에서 Vibrio spp. (V. harveyi, V. ichthyoenteri, Vibrio sp.)가 가장 우점종으로 검출된 세균의 위치를 차지하였으며, 참돔에서는 Vibrio sp.와 P. damselae가 우점종 세균으로 검출되었다. 조사기간 동안 가장 우점종으로 검출된 기생충은 넙치에서 스쿠티카충 (M. avidus)과 트리코디나충 (Trichodina sp.), 조피볼락에서 아가미흡충 (M. sebastes), 참돔에서 아가미흡충 (M. tai), 새우에서 섬모충 (Zoothamnium sp.) 으로 나타났다.

• 한국어병학회지
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• 제25권3호
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• pp.221-229
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• 2012

교잡종, 참돔 및 긴키돔은 수온이 $19.7^{\circ}C$에서 $24.0^{\circ}C$로 상승하는 7월부터 8월에 가장 높은 성장률을 나타낸 반면, 감성돔은 수온이 $23.0^{\circ}C$에서 $19.9^{\circ}C$로 하강하는 9~10월에 가장 높은 성장률을 나타내었다. 생존율은 조사기간 동안 교잡종이 가장 높은 것으로 조사되었지만 이러한 높은 생존율이 잡종 강세의 특성에 의한 것인지에 다른 요인에 의한 결과인지는 추후 자세한 연구가 필요할 것으로 생각된다. 감성돔과 긴키돔에서 녹간증이 관찰되었으나, 교잡종에서 녹간증은 나타내지 않았다. 아가미흡충 감염은 교잡종 0~90%, 참돔 20~100%, 감성돔 10% (10월), 긴키돔 20~100%로 유의적인 차이는 없었지만 교잡종에서 다소 낮은 편이었다.

• Fisheries and Aquatic Sciences
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• 제20권6호
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• pp.11.1-11.8
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• 2017

Juvenile black sea bream, Acanthopagrus schlegelii, were exposed to waterborne zinc (Zn) at concentrations of 0, 200, and $400{\mu}g/L$, at temperatures of 18 or $26^{\circ}C$ for 4 weeks. Superoxide dismutase (SOD) activities in the liver and gill of A. schlegelii significantly increased following exposure to waterborne Zn. Significant reduction in glutathione S-transferase (GST) activity in the liver and gill was observed following exposure to waterborne Zn. Glutathione (GSH) concentrations in the liver and gill also significantly decreased following exposure to waterborne Zn. Phagocytosis and lysozyme in the plasma and kidney were significantly increased following exposure to waterborne Zn. High water temperature increased alterations in the antioxidant and immune responses. The results of the present study suggest that waterborne Zn induced significant alterations in oxidative stress, increased immune responses and high temperature that trigger Zn toxicity.

• 한국양식학회지
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• 제21권1호
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• pp.7-12
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• 2008

본 연구는 시판되는 10종의 소독제(hydrogen peroxide, sodium hypochlorite, chlorine dioxide, povidon iodine, formaldehyde, glutaraldehyde, quaternary ammonium compounds(QAC), didecyl dimethyl ammonium chloride(DDAC), ortho-dichlorobenzene, and copper sulfate)에 대한 CHSE-214 cell line과 넙치 Paralichthys olivaceus, 조피볼락 Sebastes pachycephalus, 감성돔 Acanthopagrus schlegelii에 대한 24 h $LC_{50}$ 농도를 측정하였다. 시험에 사용된 시판용 소독제에 대한 CHSE-214 cell line의 24 h 독성 농도는 시판액을 기준으로 hydrogen peroxide 0.955 ppm, sodium hypochlorite 12.5 ppm, chlorine dioxide 62.5 ppm, povidon iodine 50 ppm, formaldehyde 4 ppm, glutaraldehyde 1 ppm, quaternary ammonium compounds 10 ppm, didecyl dimethyl ammonium chloride 0.4 ppm, ortho-dichlorobenzene 6.2 ppm 이었다. 10종의 소독제의 독성 농도는 어종에 뿐만 아니라 각 소독제에 따라서도 매우 다양하게 관찰되었다. Hydrogen peroxide는 소독제 중에서 가장 높은 농도에서 어류에 대한 독성을 보였는데 넙치, 조피볼락 및 감성돔에 각각 201, 269 및 139 ppm에서 $LD_{50}$을 나타내었다. DDAC는 가장 낮은 농도에서 어류에 대한 독성을 보였는데 넙치, 조피볼락 및 감성돔에서 각각 2.1, 1.0 및 1.5 ppm에서 $LD_{50}$을 나타내었다. Copper sulfate는 어종에 따라 독성을 보이는 농도가 가장 크게 나타났다.

• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 1996년도 수산관련공동학술대회발표요지집
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• pp.223-225
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• 1996

• 수산해양교육연구
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• 제27권4호
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• pp.1184-1193
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• 2015

Monitoring for megalocytivirus infection was conducted for ten months from March to December in 2013 in 15 aquatic farms culturing, red sea bream, rock bream, rock fish and black sea bream around Tongyoung coastal area in Korea, to assess spatial and temporal variability of detection prevalence, and to explore possible links with seawater temperature. In nested-PCR targeted major capsid protein (MCP) gene, asymptomatic megalocytivirus infection was detected in the externally healthy farmed fish with a significant prevalence in range from 0 to 58.3% for ten months. Higher prevalence of megalocytivirus (46.7% - 57.1%) was observed in high water temperature season from September to November than that in other months with lower prevalence of 0.0% to 20.0%. Even though an acute infection of megalocytivirus was occurred in rock bream (positive in the first PCR) with high mortality in one of fifteen farms, there was no expansion or transmission of the disease to the rock fish and red sea bream culturing in net cage just proximal to the rock bream cage in which disease outbreaked. Nucleotide sequence analysis of the cloned MCP gene isolated asymptomatically infected rock fish revealed that the megalocytivirus in this study was clustered together with the rock bream iridovirus (RBIV) under the subgroup II of the genus megalocytivirus (Iridoviridae), which is known to be the major megalocytivirus strain in Korea. The typical histopathological signs were not found in the spleen of rock fish asymptomatically infected by megalocytivirus. Experimental infection of rock bream with the spleen homogenate of the rock fish infected asymptomatically did not induce any mortality unlike the homogenate of infected rock bream with hih mortlity. However, these results may suggest that the asymptomatic infection of megalocytivirus in other fish species can be a potential risk threatening aquaculture industries as a transmission factor of megalocytivirus to susceptible fish species, especially rock bream.

• 한국수산과학회지
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• 제48권1호
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• pp.64-70
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• 2015

We investigated the branchial osmoregulatory response of black sea bream Acanthopagrus schlegelii to short-term (3-48 h) exposure to a hyposaline environment (5 psu). Gill $Na^+/K^+$-ATPase (NKA) activity was decreased after 3 h in fish transferred to 5 psu compared to salt water-acclimated (control) fish, but the level of activity returned to that observed in the control fish at 6 h after transfer. NKA activity increased significantly at 24 h after transfer, but it returned to the level observed in the control fish at 48 h after transfer. Immunohistochemical staining revealed that gill NKA was localized to chloride cells. The number of chloride cells tended to change in parallel with NKA activity. Substantial decreases in plasma $Na^+$, $Cl^-$, and osmolality were observed after 12 h of exposure to 5 psu; however, these parameters began to recover to the values detected in the controls at 24 h after transfer. In conclusion, our results suggest that black sea bream are able to adjust their osmoregulatory mechanisms to shift from hypo- to hyperosmoregulation within 6 h of exposure to a hypoosmotic environment.

• 한국양식학회:학술대회논문집
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• 한국양식학회 2005년도 춘계학술발표대회 논문요약집
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• pp.85-85
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• 2005

• 한국수산과학회지
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• 제41권1호
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• pp.20-25
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• 2008

The susceptibility of five different marine fish to iridovirus IVS-1 infection was analyzed and found a higher the cumulative mortality in the order of rock bream (Oplegnathus fasciatus), red sea bream (Pagrus major), sea perch (Lateolabrax sp.), rockfish (Sebastes schlegeli) and black porgy (Acanthopagrus schlegeli). However, the concentrations of virus in the infected spleens of these species did not differ significantly. To determine the release of iridovirus from infected fish into culturing seawater, rock bream were challenged with iridovirus IVS-1 and the concentration of virus in the water was analyzed using PCR. Over the 10 days of the analysis, the linear relationship between the number of dead fish and viral DNA concentration found in culturing seawater should be considered direct evidence of horizontal iridovirus transmission.

• Food Science and Biotechnology
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• 제17권5호
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• pp.971-975
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• 2008

This study was to examine the potential disinfection efficiencies of 10 compounds by determining their antimicrobial capacity and ichthyotoxicity. Antimicrobial effects against Vibrio sp., Edwadsiella tarda, Streptococcus sp., and Staphylococcus sp. were tested using 10 different disinfectants; hydrogen peroxide, sodium hypochlorite, chlorine dioxide, povidon iodine, formaldehyde, glutaraldehyde, quaternary ammonium compounds (QACs), didecyl dimethyl ammonium chloride (DDAC), ortho-dichlorobenzen, and copper sulfate. Chlorine dioxide ($ClO_2$) containing 5% $ClO_2$ and copper sulfate had no effects on bactericidal activity, while the other disinfectants resulted in 99.99% bactericidal activity against 4 strains of fish pathogenic bacteria. The ichthyotoxicity of the 10 disinfectants was investigated using 3 kinds of fish species; flounder (Paralichthys olivaceus), rockfish (Sebastes pachycephalus), and black sea bream (Acanthopagrus schlegelii). Median lethal concentration ($LC_{50}$) values of the 10 disinfectants were estimated to determine toxicity ranges of the doses within 24 hr. Among test disinfectant solutions, hydrogen peroxide showed the highest $LC_50$ in flounder (201.3), rockfish (269.7), and black sea bream (139.3 ppm). DDAC revealed the lowest $LC_{50}$ in flounder (2.1), rockfish (1.0), and black sea bream (1.5 ppm). These results suggest that DDAC, quaternary ammonium compounds, glutaraldehyde, and sodium hypochlorite are effective disinfectants for fish and bacterial species examined in this study.