• Title/Summary/Keyword: Bivoltine silkworm

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Estimation of Heterosis and Combining Ability in Hybrids between Resistant and Susceptible Bivoltine Breeds of Silkworm Bombyx mori to Densonucleosis Virus1 (BmDNV1)

  • Rao, P. Sudhakara;Nataraju B.;Balavenkatasubbaiah M.;Dandin S.B.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.13 no.2
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    • pp.73-77
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    • 2006
  • Combining ability and hybrid vigour analysis was carried out in hybrids between newly developed non-susceptible lines to BmDNV1 and popular bivoltine breeds for certain quantitative traits viz. Pupation rate, Cocoon yield, Cocoon weight, Cocoon shell weight and Cocoon shell ratio, Survival rate against BmIFV and BmNPV. General combining ability (GCA) effects revealed that among the lines CSR2DR was found good general combiner exhibiting significant GCA effects for six characters, out of seven traits evaluated. Among testers CSR28DR was found as good combiner exhibiting significant GCA effects for six traits. Out of 36 hybrids made between $resistant{\times}resistant,\;resistant{\times}susceptible\;and\;susceptible{\times}susceptible$ breeds, one hybrid $CSR21DR{\times}CSR28DR$ exhibited significant SCA effects for six traits. The selected hybrid $CSR21DR{\times}CSR28DR$ also exhibited significant positive heterosis and heterobeltiosis expressions for maximum traits and could be exploited as commercial silkworm hybrid resistant to important viral diseases.

Breeding Resource Materials of Silkworm Bombyx mori L., Adaptive to Tropical Climates

  • P. Sudhakara Rao;R. K. Datta;K. M, Vijaya-Kumari;M. Ramesg Babu
    • International Journal of Industrial Entomology and Biomaterials
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    • v.4 no.2
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    • pp.109-115
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    • 2002
  • With the objective of selecting suitable breeding resource material, 10 polyvoltine and 10 bivoltine breeds were drawn from the germplasm collection of Central Sericultural Research and Training Institute, Mysore, and evaluated for 3 seasons comprising one year (6 trials). Data were collected on seven traits of economic importance such as fecundity, pupation rate, cocoon yield, cocoon weight, cocoon shell weight, cocoon shell ratio and filament length, and statistically analysed with two-way classification, Joint scoring method and evaluation index. Significant seasonal variations (P < 0.01) were observed in both polyvoltine and bivoltine breeds. Polyvoltines BL27, BL36 and BL54 and bivoltines CSR2, CSR4 and Daizo scored highest ranking values in all the three methods. Hybridization was initiated based on larval markings and cocoon shapes. Seasonal variations were discussed.

Development of Fine Denier Silkworm Hybrid $CSR48{\times}CSR5$ of Bombyx mori L. for Superior Quality Silk

  • Kalpana G. V.;Kumar N. Suresh;Basavaraja H. K.;Reddy N. Mal;Palit A. K.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.10 no.2
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    • pp.147-151
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    • 2005
  • Serious attempt for the improvement of quality parameters in silkworm hybrids came rather very late in India. Realising the need for productive breeds to the field and reorientation in breeding methodology, the silkworm breeders of CSRTI, Mysore were able to develop silkworm breeds with fine denier namely, JPN7, CSR48, B63 and B65 of specific industrial requirement through directional selection. As the success of silkworm breeds mainly depends on their combining ability, the developed breeds were subjected for hybrid evaluation. Out of 42 bivoltine hybrids studied, one hybrid, $CSR48{\times}CSR5$ was identified for its superiority over the existing bivoltine hybrids namely $CSR2{\times}CSR4,\;CSR2{\times}CSR5\;and\;CSR18{\times}CSR19$ for majority of qualitative traits. Post cocoon testing of large quantity of cocoons of $CSR48{\times}CSR5$ resulted in the production of high quality '3A'grade silk. It is for the first time in the Indian Sericulture industry that high quality silk with longer filament length of 1474 m, denier of 2.47, standard size deviation of 0.983 d, maximum size deviation of 1.322, neatness of 96 p, reelability of $85\%$, tenacity of 3.87 g/d, elongation of $22.32\%$ and cohesion of 110 strokes was produced. The superiority of fine denier silkworm breeds and their hybrids are discussed.

Studies on Determination of Larval Critical Weight in Silkworm, Bombyx mori L. - An Index of Quality

  • Saha, Atul Kumar;Chaudhuri, Anath Bandhu;Moorthy, S Monthira;Roy, Subrata
    • International Journal of Industrial Entomology and Biomaterials
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    • v.19 no.2
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    • pp.243-248
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    • 2009
  • The Larval critical weight is the minimal mass at which further growth in not necessary for a normal time course to pupation. Larval critical weight (Lcw), pupal critical weight (Pcw) and adult critical weight (Acw) of different breeds of Bombyx mori L. were determined through starvation (food deprivation). The Lcw was found to be about 938.46, 2397.26, 2283.57 and 2220.97 mg in males and 1118.15, 2681.04, 2604.9 and 2455.88 mg in females of the multivoltine breed (Nistari), Bivoltine breeds (P5 & NB18) and their hybrid (P5$\times$NB18) respectively. Bivoltine breed P5 took more time (3.35 days) followed by NB18 (3.13 days) & P5$\times$NB18 (3.02 days) to attain larval critical weight (In $5^{th}$ Instar) than the multivoltine breed Nistari (2.42 days). Decrease in weight from larval maximal weight to pupal weight and to adult weight was also observed more in multivoltine than bivoltines, which may be due to more latent feeding period in bivoltines. Since Lcw is a stable character and independent of environment, it could be utilized for characterization of silkworm breeds to assess the quality of an insect.

Studies on the Manifestation of Hybrid vigour and Combining Ability in Polyvoltine X Bivoltine (cross breed) Hybrids of Mulberry Silkworm Bombyx mori L.

  • Seshagiri, S.V.;Naik, S. Sankar;Seetharamulu, J.;Goel, A.K.;Raju, P.J.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.24 no.1
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    • pp.7-16
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    • 2012
  • To meet the local requirement i.e. the tropical stress of Andhra Pradesh and marginal farmers, twenty four new hybrid combinations were tested in Line x Tester method involving eight newly developed polyvoltine oval lines viz., MSO1, MSO2, MSO3, MSO4, MSO5, MSO6, MSO7 and MSO8 and three bivoltine silkworm breeds viz., APS12, APS45 and APS8 as testers. These hybrids were reared to evaluate their performance. Data for eight economically important metric traits viz., fecundity, cocoon yield per 10,000 larvae by number, cocoon yield per 10,000 larvae by weight, survival rate, cocoon weight, cocoon shell weight, cocoon shell ratio and filament length was recorded. General combining ability (gca), specific combining ability (sca) and heterosis were analyzed. Further, the relative merit of the hybrids for all the traits under study was also assessed adopting Evaluation Index method. Based on combining ability effects, heterosis and evaluation index, five hybrid combinations viz., MSO3 $\times$ APS45, MSO1 $\times$ APS12, MSO7 $\times$ APS8, MSO2 $\times$ APS45 and MSO1 $\times$ APS45 were selected for further evaluation in different seasons.

Influence of Thyroxine on the Economic Parameters of the Bivoltine Silkworm, Bombyx Mori L.

  • Hugar, I.I.;Kaliwal, B.B.
    • Journal of Sericultural and Entomological Science
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    • v.38 no.2
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    • pp.130-137
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    • 1996
  • Effect of topical application with 5, 10 and 15${\mu}$g/ml thyroxine on the pre-cocoon, cocoon, postcocoon and silk improvement were studied in bivoltine silkworm NB18 breed of B. mori L. Thyroxine was applied independently on alternate days to silkworm of IV and V instar larvae. The treatment of all different concentrations resulted in a significant increase in commercial characters like larval weight, silkgland weight, cocooning percentage, female cocoon weight and its shell weight and male cocoon shell weight and its ratio, cocoon dimension, length of the filament and its weight and oviposition. However, there was no significant change in larvel weight in 5${\mu}$g/ml treated group and larval duration was significantly decrease. But moth emergence percentage and hatching percentage did not show any significant change when compared with that of carrier control.

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Boil-off Loss Ratio of Cocoon Shell as a Selection Criterion in the Newly Developed Bivoltine Silkworm (Bombyx mori L.) Hybrids

  • Seetharamulu, J.;Seshagiri, S.V.;Raju, P.J.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.26 no.1
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    • pp.13-21
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    • 2013
  • Boil-off loss ratio is one of the most important economic traits to be considered during the course of silkworm breeding. The boil-off loss ratio varies among the breeds and seasons. The present study was undertaken with 5 oval and 5 peanut breeds and twenty five hybrids involving these parents to select the promising hybrids with desired boil-off loss ratio. Accordingly, studies were undertaken to estimate the heterosis and heterobeltiosis. It was observed that majority of the hybrids revealed negative heterosis for boil-off loss ratio which is desirable. Based on the heterosis and heterobeltiosis, among all the hybrids, the hybrid APBRO5 ${\times}$ APBRD5 exhibited highest negative heterosis for boil-off loss ratio and also exhibited highest EI value (64.5) for the quantitative and qualitative traits. Based on the hybrids performance, boil-off loss ratio, heterosis and heterobeltiosis and evaluation index, the hybrids viz. APBRO5 ${\times}$ APBRD5, APBRO1 ${\times}$ APBRD1 and APBRO3 ${\times}$ APBRD4 were identified for commercial exploitation.

Heterosis Studies in Some Elite Multivoltine Silkworm (Bombyx mori L.) Races with Popular Bivoltine N$B_4D_2$

  • Kumaresan, P.;Sinha, R.K.;Thangavelu, K.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.7 no.2
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    • pp.221-229
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    • 2003
  • Ten multivoltine female parental lines were crossed with popular bivoltine male silkworm breed NB$_4$D$_2$. Three types of heterosis parameters viz., heterosis over mid-parental value (hybrid vigour), heterobeltiosis (useful heterosis) and standard heterosis (standard check) were estimated for 15 economically important quantitative traits. The interaction among the hybrids and parents indicated significant effect for maximum characters. The heterotic effect of new hybrid combination was compared with popular hybrids viz., Pure Mysore${\times}$NB$_4$D$_2$ and Nistari${\times}$NB$_4$D$_2$. Varied heterotic effect was observed for different traits for different hybrid combination. The results inferred that the crosses viz., BL$_{23}$${\times}$NB$_4$D$_2$ ranked top for 14 traits followed by Hosa Mysore${\times}$NB$_4$D$_2$ for 11 traits; PA$_{12}$${\times}$NB$_4$D$_2$ for 9 traits; BL$_{24}$${\times}$NB$_4$D$_2$ for 8 traits; Kolar Gold${\times}$NB$_4$D$_2$ for 7 traits; WAI$_1$${\times}$NB$_4$D$_2$ for 6 traits and MU$_{11}$${\times}$NB$_4$D$_2$ for 5 traits. Among these, the best hybrids Kolar Gold${\times}$NB$_4$D$_2$ and MU$_{11}$${\times}$NB$_4$D$_2$ were identified for longer filament length and fine denier. Similarly for higher cocoon yield and silk productivity BL$_{223}$${\times}$NB$_4$D$_2$ and BL$_{24}$${\times}$NB$_4$D$_2$ were found to be superior. These hybrid combinations are suitable for commercial exploitation at large scale.e.e.e.

Development of a Robust Polyvoltine Breed $'NP_1'$ of the Mulberry Silkworm, Bombyx mori L.

  • Singh Ravindra;Rao D. Raghavendra;Sharma S.D.;Chandrashekaran K.;Basavaraja H.K.;Kariappa B.K.;Dandin S.B.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.12 no.1
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    • pp.29-34
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    • 2006
  • A breeding programme was initiated during 2001 utilizing two polyvoltine silkworm breeds viz. $BL_{69}$, an evolved breed tolerant to high temperature and MAR, comparatively resistant to Bombyx mori nuclear polyhedrosis virus (BmNPV) with the objective to develop robust polyvoltine breeds and hybrids. The breed $NP_1$ was developed by exposing the fifth instar larvae to high temperature $(36{\pm}1^{\circ}C)$, high Relative Humidity ($85{\pm}5%$ R.H.) and inoculating third instar larvae with BmNPV inoculum. At $F_{12}$, the breed was tested for hybrid forming ability utilizing six bivoltine silkworm breeds viz. $CSR_2,\;CSR_4,\;CSR_{17},\;CSR_{18},\;CSR_{19}\;and\;NB_4D_2$. The hybrid $'NP_1{\times}CSR_{17}'$ exhibited its superiority by recording 97.2% survival, 1.892 g cocoon weight, 0.406 g cocoon shell weight, 21.5% cocoon shell ratio, 16.6% raw silk percentage and 890 m filament length whereas the control $(PM{\times}CSR_2)$ has recorded 90.2% survival, 1.599 g cocoon weight, 0.304 g cocoon shell weight, 18.9% cocoon shell ratio, 13.1 % raw silk percentage and 768 m filament length. Commercial exploitation of the new $polyvoltine{\times}bivoltine$ hybrid in sericulture industry has been discussed.