• Journal of Applied Biological Chemistry
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• v.53 no.1
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• pp.60-64
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• 2010

None of genes from Lespedeza bicolor involved in isoflavonoid biosynthesis have been biochemically characterized. An isoflavone synthase from Lespedeza bicolor was cloned. To verify its catalytic activity, a fusion protein of LbIFS with P450 reductase from rice was made. Using this construct, production of isoflavone from flavanone was confirmed.

• Journal of Korea Soil Environment Society
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• v.4 no.3
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• pp.35-43
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• 1999

Biotransformation of inorganic nitrogens, which are possible contaminants of bank filtered water, in soil of near bank filtration site was investigated based on oxygen consumption and changes of chemical parameters in respirometer. Biotransformation activities of inorganic nitrogens at different conditions of pH. water content. and added initial${NH_4}_2$$SO_4$were compared. At original low pH and 20% of water content, nearly no biotransformation activity of inorganic nitrogen was observed, in addition, control and NH$_4$-added sets did not show any significant differences of oxygen consumption. Among tested conditions, the highest activity was observed at 25% water content and pH 8. Nearly 98% nitrification activity was observed at sets amended with 400 mg $NH_4$-N/kg soil as${NH_4}_2$$SO_4$in the condition of pH 8 and 20~23% water content. However, considerable activity of subsequent denitrification was not observed.