• Title/Summary/Keyword: Bioscience

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The PKA/CREB Pathway Is Closely Involved in VEGF Expression in Mouse Macrophages

  • Jeon, Seong-Hyun;Chae, Byung-Chul;Kim, Hyun-A;Seo, Goo-Young;Seo, Dong-Wan;Chun, Gie-Taek;Yie, Se-Won;Eom, Seok-Hyun;Kim, Pyeung-Hyeun
    • Molecules and Cells
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    • v.23 no.1
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    • pp.23-29
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    • 2007

  • Cyclic AMP-responsive element binding protein (CREB) is known to be associated with angiogenesis. In the present study we investigated the possible role of CREB in the expression of vascular endothelial growth factor (VEGF) by mouse macrophages. Over-expression of CREB increased VEGF secretion by cells of the RAW264.7 mouse macrophage cell line. It also increased the promoter activity of a mouse reporter driven by the VEGF promoter, while a dominant negative CREB (DN-CREB) abrogated the activity, suggesting that CREB mediates VEGF transcription. Forskolin, an adenylyl cyclase activator, stimulated VEGF transcription, and the PKA inhibitor H89 abolished this effect. IFN-${\gamma}$, a potent cytokine, stimulated VEGF expression only in part through the PKA-CREB pathway. These results indicate that PKA phosphorylates CREB and so induces VEGF gene expression. An analysis of mutant promoters revealed that one of the putative CREB responsive elements (CREs), at -399 ~ -388 in the promoter, is critical for CREB-mediated VEGF promoter activity, and the significance of this CRE was confirmed by chromatin immunoprecipitation assays.

  • KSCI

Introduction of the Korea BioData Station (K-BDS) for sharing biological data

  • Byungwook Lee;Seungwoo Hwang;Pan-Gyu Kim;Gunwhan Ko;Kiwon Jang;Sangok Kim;Jong-Hwan Kim;Jongbum Jeon;Hyerin Kim;Jaeeun Jung;Byoung-Ha Yoon;Iksu Byeon;Insu Jang;Wangho Song;Jinhyuk Choi;Seon-Young Kim
    • Genomics & Informatics
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    • v.21 no.1
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    • pp.12.1-12.8
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    • 2023

  • A wave of new technologies has created opportunities for the cost-effective generation of high-throughput profiles of biological systems, foreshadowing a "data-driven science" era. The large variety of data available from biological research is also a rich resource that can be used for innovative endeavors. However, we are facing considerable challenges in big data deposition, integration, and translation due to the complexity of biological data and its production at unprecedented exponential rates. To address these problems, in 2020, the Korean government officially announced a national strategy to collect and manage the biological data produced through national R&D fund allocations and provide the collected data to researchers. To this end, the Korea Bioinformation Center (KOBIC) developed a new biological data repository, the Korea BioData Station (K-BDS), for sharing data from individual researchers and research programs to create a data-driven biological study environment. The K-BDS is dedicated to providing free open access to a suite of featured data resources in support of worldwide activities in both academia and industry.

  • https://doi.org/10.5808/gi.22073 인용 PDF