• Title/Summary/Keyword: Biomaterial

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Hardness of Ti alloys by mechanical processing methods (Ti 합금의 기계가공 방법에 따른 경도 변화에 관한 연구)

  • 반재삼;김규하;정상원;기강호;조규종
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2002.10a
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    • pp.792-795
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    • 2002
  • In previous researches, it is reported that Ti-10Ta-10Nb is robuster than Ti-6A1-4V which is used as a biomaterial in a experiment of cytotoxicity. Ti-10Ta-10Nb has enough hardness to be required as a biomaterial because the change of its hardness can be controlled more than 100% according to heat treatment condition and manufacturing condition. There are many hardness changing condition including Cast Homogenization, Solution treatment. Forging, Rolling in this research. The changing form and amount of new Ti-10Ta-10Nb to be developed in this researches, are measured as quantitative. Specially, the changing hardness amount of the specimen that is manufactured in single phase temperature, i.e. 80$0^{\circ}C$, are measured in case of high temperature rolling and high temperature cast condition.

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Dihydrodaidzein production from soybean hypocotyl extract by human intestinal bacterium MRG-1

  • Sirirat Prasertwasu;Jaehong Han
    • Journal of Applied Biological Chemistry
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    • v.65 no.4
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    • pp.447-451
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    • 2022
  • Phytoestrogenic S-equol production in human gut exclusively depends on the biotransformation of daidzein to dihydrodaidzein (DHD). With a growing demand for the DHD enriched biomaterials, the commercial soybean hypocotyl extract (SHE) was chosen as a substrate for the microbial DHD production by human gut bacterium MRG-1, anaerobic DHD producer. To optimize the production of DHD, anaerobic fermentation conditions, including sterilization time, growth stage of inoculum, and growth media, were investigated. Maximum DHD production (1.2 g/L) was achieved after 48 h incubation when 1% (w/v) of SHE in the 20-min-sterilized Gifu Anaeboic Medium media was inoculated with OD600 0.3-0.4 of MRG-1. This is the first report that crude soy biomaterial, instead of pure compounds, such as daidzin and daidzein, is utilized for the production of the DHD enriched biomaterial.

Effect of temperature and denaturation conditions on protein folding assisted by GroEL-GroES chaperonin (GroEL-GroES 샤페로닌에 의한 단백질 접힘에 있어서 온도와 변성조건의 영향)

  • Bae, Yu-Jin;Jang, Kyoung-Jin;Jeon, Sung-Jong;Nam, Soo-Wan;Lee, Jae-Hyung;Kim, Young-Man;Kim, Dong-Eun
    • Journal of Life Science
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    • v.17 no.2 s.82
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    • pp.211-217
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    • 2007
  • The goal of this study is to investigate effects of temperature and co-chaperonin requirement for in vitro protein refolding assisted by E. coli chaperone GroEL under permissive and nonpermissive temperature conditions. In vitro protein refolding of two denatured proteins was kinetically investigated under several conditions in the presence of GroEL. Effects of temperature and GroES-requirement on the process of prevention of protein aggregation and refolding of denatured protein were extensively monitored. We have found that E. coli GroEL chaperone system along with ATP is required for invitro refolding of unfolded polypeptide under nonpermissive temperature of $37^{\circ}C$. However, under permissive condition spontaneous refolding can occur due to lower temperature, which can competes with chaperone-mediated protein refolding via GroEL chaperone system. Thus, GroEL seemed to divert spontaneous refolding pathway of unfolded polypeptide toward chaperone-assisted refolding pathway, which is more efficient protein refolding pathway.

The Development of New Biomaterial for Restoration of Hard Tissue Defects (경조직 결손 수복을 위한 새로운 생체재료의 개발)

  • Kim, Young-Kyun
    • The Journal of the Korean dental association
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    • v.36 no.4 s.347
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    • pp.289-295
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    • 1998
  • Toothash and Plaster of Paris(Calcium sulfate) mixture have been studied for development of new biomaterial since 1992. I have performed the experimental and clinical study for evaluation of biocompatibility and possibility of bony defect reconstruction and clinical application. These materials are biocompatible, osteoconductive and resorbable. Main component of toothash is hydroxylapatite. In the future, I will perform the systematic construction of material and additional research.

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