• Title/Summary/Keyword: Biology I

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Postembryonic Development of Leucokinin I-Producing Neurons in the Brain of Insect Spldoptera litura

  • Kang, Hyu-No;Lee, Bong-Hee
    • Animal cells and systems
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    • 제1권1호
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    • pp.107-113
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    • 1997
  • Antisera against the myotropic neuropeptide leucokinin I, originally isolated from head extracts of the cockroach Leucophaea maderae, have been used to investigate the distribution of the leucokinin I-immunoreactive (LK I-IR) neurons in the brain of the common cutworm, Spodoptera Iitura, during postembryonic development. The LK I-IR neurons are found at the larval stages (excluding first instar larval stage), pupal stages, and adult stage, of which the brains have been examined in this experiment. The number of the LK I-IR neurons in the brain increases from the second instar larva to the fifth instar larva which has about 32, the largest number in all postembryonic stages. Thereafter, the LK I-IR neurons begin to decrease in number. During the pupal stages, smaller number of LK I-IR neurons persist in the brains; 6 or 4. At adult stage the brain contains 8 LK I-IR neurons. The LK I-IR cell bodies are distributed in each dorsal cortex of both cerebral hemispheres in the second instar larva and through all the neuromeres of the brain during later larval stages, despite of being a large number of the LK I-IR cell bodies in dorsolateral neuromeres. At pupal stages, most of the LK I-IR cell bodies are found in the pars intercerebralis. Extremely small number of the LK I-IR cell bodies are localized in the pars lateral is. Adult brain contains the LK I-IR cell bodies in the pars intercerebralis and the middle cortex of the posterior brain. The LK I-IR nerve processes can be easily found in the neuropils of almost all the neuromeres in the brains of third, fourth, fifth and sixth instar larvae. Most of the LK I-IR nerve fibers in those brains are originated from the LK I-IR cell bodies located in the brains. The LK I-IR cell bodies which have very weak reactivities to the antisera do not show projection of the LK I-IR nerve processes in the brains.

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베트남산 납작하루살이류 2종, Iron martinus Braasch and Sold${\acute}$n 및 Iron longitibius New Species (하루살이목: 납작하루살이과) (Two Heptageniid Mayflies, Iron martinus Braasch and Sold${\acute}$n and Iron longitibius New Species (Ephemeroptera: Heptageniidae), from Vietnam)

  • 원빈;배연재
    • 생태와환경
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    • 제37권1호통권106호
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    • pp.102-105
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    • 2004
  • 베트남산 납작하루살이류 2종 (Iron martinus Braasch and Sold${\acute}$n 및 I.longitibius new species)의 유충을 기재하였다. Iron martinus유충은 1${\sim}$9 배마디에 있는 한쌍의 등가시에 의하여 구별되고, I.longitibius 유충은 상대적으로 긴 앞다리 종아리마디에 의하여 구별된다. 그들의 기재, 진단 형질, 검색 형질의 그림, 관찰한 표본, 분포, 서식처 및 생물 자료를 제시하였다.

Purification and Characterization of Glyoxalase I from Chlamydomonas reinhardtii

  • Hwang, Sun-Jun;Chai, Young-Gyu
    • BMB Reports
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    • 제29권4호
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    • pp.294-299
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    • 1996
  • Glyoxalase I (Ee 4.4.1.5, lactoylglutathione lyase) from Chlamydomonas reinhardtii was purified to homogeneity by ammonium sulfate fractionation, anion-exchange chromatography, hydrophobic interaction chromatography, and affinity chromatography on S-hexylglutathione agarose. The purified enzyme was judged to be homogeneous on SDS-PAGE, and consisted of a single polypeptide chain with a relative molecular weight of 24,000. The enzyme was most active at $40^{\circ}C$ and pH 7.5. It was catalytically most active with methylglyoxal as substrate. A number of properties of the Chlamydomonas glyoxalase I enzyme, such as substrate specificity, molecular mass, kinetic parameters, pi, metal ion effect, have been determined and compared with those reported for preparations from other sources. It had somewhat different characteristics from mammalian enzymes.

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Preservice Biology Teachers' Learning to Teach Science through Science Methods Courses

  • Kim, Sun Young
    • 한국과학교육학회지
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    • 제32권9호
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    • pp.1427-1442
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    • 2012
  • This study tracked the changes of preservice biology teachers' pedagogical knowledge along with science teaching efficacy throughout sequentially developed science methods course I and II over two consecutive semesters. Two courses, science methods course I and II, aimed these preservice teachers to discuss the notion of science teaching with teaching and learning theories, to learn science instructional models, to design lessons utilizing science instructional models, and to eventually implement microteaching. The preservice teachers were mainly engaged in cooperative instructional planning activities through science methods course I, and engaged in cooperative microteaching activities through the science methods course II. This study revealed that preservice teachers successfully developed pedagogical knowledge and science teaching efficacy after two science methods courses. The science methods course I where cooperative instructional planning activities occurred helped the preservice teachers to improve pedagogical knowledge but not science teaching efficacy. Based on their pedagogical knowledge development, then, these preservice teachers increased science teaching efficacy belief after completion of the science methods course II.

DR (Digital Radiography) 적용을 위한 Biology 초음파 특수용매를 이용한 $PbI_2$ 합성법

  • 김성헌;윤민석;오경민;김영빈;이상훈;조규석;박혜진;남상희
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2009년도 추계학술대회 논문집
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    • pp.146-146
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    • 2009
  • 최근에 광도전체와 형광체를 기반으로 평판형 디지털 방사선 검출기의 상업적 발전가능성에 많은 관심을 가지고 있다. 본 연구는 기존의 직접변환방식에 널리 사용되었던 비정질 셀레늄 (amorphous selenium) 기반의 디지털 방사선 검출기보다 높은 전기적신호 및 동작특성을 가지는 물질층을 제작하기 위해 High Purity (99.99%)의 상용화된 $PbI_2$를 특수용매에 담가두었다가 약 1시간동안 Biology 초음파 처리한 후 농축기를 사용하여 건조된 $PbI_2$를 3Roll-milling을 사용하여 미세크기의 Powder를 얻어내었다. 합성된 $PbI_2$ Powder를 PIB(Particle-in-Binder)법을 이용하여 전도성을 가진 ITO(Indium-tin-oxide)코팅된 유리판에 제작된 필름의 상부에 Magnetron sputtering system 을 사용하여 전극을 $1cm{\times}1cm$의 크기로 증착하였다. I-V 테스트를 통하여 X선 조사시 $PbI_2$필름의 Sensitivity, Dark current, SNR(signal-to-noise ratio)을 측정하여 필름의 전기적 검출 특성을 정량적으로 평가하였고 SEM(scanning electron microscope)을 통하여 입자의 크기를 관찰하였다.

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생쥐의 지단백질과 조직단백질에 미치는 메틸수은 독성에 대한 홍삼의 영향 (Effects of Red Ginseng on Methyl Mercury Toxicities to Lipoprotein and Tissue Protein in Mouse)

  • Chung, Hee Won;Soo Kyoung Shin;Choon Koo Lee
    • The Korean Journal of Ecology
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    • 제10권4호
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    • pp.205-213
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    • 1987
  • In order to investigate the effects of red ginseng extract to methyl mercury toxicities in mice, the serum lipoproteins, tissure protein patternsm and growth rates were studied. Animals wee divided into 3 groups of the control, group I treated with methyl mercury chloride only, and group II treated together with methyl mercury chloride and red ginseng extract. In serum lipoprotein fractions of group I, beta lipoprotein fraction was increased and pre-beta lipoprotein fraction was decreased in comparision to those of the control. However, there was almost no difference in quantities of serum lipoprotein fractions between the control and group II. Total pretein contents of groups I and II were increased in liver and those of groups I and II in the kidney were decreased. However, in comparison to group I, total protein contents of group II in the liver and kidney were similar values with those of the control. Percentage of tissue protein fractions between control and group I in the liver and kindey showed considerable difference. On the other hand, the percentage of protein fractions of group II approximated to that of the control. Daily average growth rate of body weight in group II was similar to the control, but that of group I was decreased significantly in comparison to the other 2 groups.

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