• Interdisciplinary Bio Central
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• 제1권3호
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• pp.9.1-9.6
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• 2009

Introduction: In the mass spectrometry-based proteomics, biological samples are analyzed to identify proteins by mass spectrometer and database search. Database search is the process to select the best matches to the experimental mass spectra among the amino acid sequence database and we identify the protein as the matched sequence. The match score is defined to find the matches from the database and declare the highest scored hit as the most probable protein. According to the score definition, search result varies. In this study, the difference among search results of different search engines or different databases was investigated, in order to suggest a better way to identify more proteins with higher reliability. Materials and Methods: The protein extract of human mesenchymal stem cell was separated by several bands by one-dimensional electrophorysis. One-dimensional gel was excised one by one, digested by trypsin and analyzed by a mass spectrometer, FT LTQ. The tandem mass (MS/MS) spectra of peptide ions were applied to the database search of X!Tandem, Mascot and Sequest search engines with IPI human database and SwissProt database. The search result was filtered by several threshold probability values of the Trans-Proteomic Pipeline (TPP) of the Institute for Systems Biology. The analysis of the output which was generated from TPP was performed. Results and Discussion: For each MS/MS spectrum, the peptide sequences which were identified from different conditions such as search engines, threshold probability, and sequence database were compared. The main difference of peptide identification at high threshold probability was caused by not the difference of sequence database but the difference of the score. As the threshold probability decreases, the missed peptides appeared. Conversely, in the extremely high threshold level, we missed many true assignments. Conclusion and Prospects: The different identification result of the search engines was mainly caused by the different scoring algorithms. Usually in proteomics high-scored peptides are selected and low-scored peptides are discarded. Many of them are true negatives. By integrating the search results from different parameter and different search engines, the protein identification process can be improved.

• 생태와환경
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• 제53권1호
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• pp.1-10
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• 2020

본 연구는 훼손된 하천 수생태계의 훼손원인을 식별하고 그 전이 과정을 규명하기 위해 수생태계 훼손원인 진단체계를 구축하고 정책적 활용 가능성을 보고자 하였다. 수생태계 현황 조사 및 건강성 평가결과는 하천의 훼손에 대한 전반적인 상황을 알 수 있어 하천 복원 및 관리의 판단 근거로 사용된다. 그러나 하천이 훼손된 원인이나 훼손이 발생하게 된 전이 과정을 고려하지 못한다는 한계를 가진다. 따라서 본 연구에서는 과학적이고 체계적인 진단체계를 제안하여 훼손원인과 그 전이 과정을 규명하였다. 미국, 호주, 유럽의 사례를 분석하여 우리나라에 맞는 진단체계를 제안하였다. 총 8단계로 구성되며, 기초자료 조사, 하천 수생태계 훼손 판단, 훼손 구간 설정, 훼손 유형화 및 잠재적 훼손원인 도출, 본 조사, 주훼손원인 진단 및 최적 훼손 모델 도출, 종합분석 및 하천 복원방안으로 이루어져 있다. 이러한 진단체계는 하천 생태계의 복잡하고 다양한 특성을 고려하여 훼손원인을 파악할 수 있으며 하천 복원 및 관리 방안을 제시하여 효율적인 의사결정을 지원할 수 있다.

• 한국습지학회지
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• 제19권3호
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• pp.271-278
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• 2017

최근 비점오염물질 처리를 위하여 저영향개발(low impact development) 기술이 적용되고 있으며, 레인가든 기술은 생물학적 및 물리화학적 처리에 의하여 비점오염물질 저감에 기여하기에 광범위하게 적용되고 있는 LID 기술 중 하나이다. 그러나 유지관리를 지속적으로 수행하지 않아 시설 내 막힘 현상 등의 문제가 발생한다. 따라서 본 연구는 효율적인 물수지 및 오염물질 저감을 위해 레인가든 기술의 구성 요소 배치의 개발 및 평가를 위하여 수행하였으며, 서로 다른 2개의 하이브리드 레인가든 시스템 구축을 통하여 시스템의 최적화된 설계 및 구성요소의 배열을 도출하였다. 분석 결과, 시스템의 구성요소를 직렬로 배열 시 저감량은 유출량의 경우 96%, 오염물질 중 입자상 물질은 99%, 유기물질은 93% 및 중금속은 95%로 나타났다. 반면 시스템이 병렬로 배열 될 시, 유출량은 65% 저감되었으며, 평균 오염물질 저감효율은 TSS는 94%, 영양물질은 80% 및 중금속은 85%으로 평가되었다. 또한, 시스템의 구성요소가 비점오염물질 저감에는 침전, 침투도랑 및 식재부의 순서가 중요한 영향인자로 나타났다. 향후 레인가든 시스템 개발 시 최적화 설계 인자로 활용 가능할 것으로 기대된다.

• 한국환경농학회지
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• 제26권4호
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• pp.300-305
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• 2007

고랭지 밭토양의 동계 피복작물재배에 따른 밭토양의 지력증진과 증수효과를 분석하기 위하여 고랭지의 주요 재배작물인 감자와 배추를 대상으로 고랭지 농업지대에 적합한 동계 피복작물을 선발하고, 피복작물재배가 토양의 물리-화학적 특성 변화에 미치는 영향, 고랭지 작물의 생산성 및 병해발생 정도를 작부체계와 연계하여 비교 검토하였다. 고랭지에서 월동성이 가장 우수한 피복작물은 17개 시험초종 중 호밀이었으며, 호밀의 적정 파종기는8월 하순부터 9월 하순으로 10월 상순 서리가 내리기 이전까지는 파종하여야 할 것으로 판단되며, 토양의 물리-화학성은 윤작과 동계 호밀재배로 토양 공극 및 유기물 함량이 증가하는 경향으로 윤작보다 동계호밀재배에 의한 토양 물리성 개선 및 토양 화학성분의 함량증가가 현저하였다. 고랭지 감자와 배추에 대하여 매년 윤작으로 작부체계를 검토한 결과 동계호밀재배는 나지에 비하여 작부체계와 관계없이 수량은 $6{\sim}8%$증가하였다. 동계 호밀재배에 의한 토양관리 방법은 토양유실 방지효과와 더불어 감자의 수확량 증대, 감자의 병해 발생률을 감소시키므로 우량 씨감자 생산을 위한 새로운 농업기술로 적용되어야 할 것으로 생각된다.

• 환경생물
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• 제17권2호
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• pp.129-138
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• 1999

난분해성 유기화합물의 일종인 염화 방향족화합물은 냉각제, 소화제, 페인트, 용매, 플라스틱류, 유압제, 제초제, 농약, 그리고 화학합성에 필요한 전구물질 등에 널리 사용된다. 이들은 친지질 특성을 가지므로 생물체의 세포막에 쉽게 흡착되며 먹이사슬에 의한 생물학적 농축과정을 통해 인간을 포함하는 각종 생물체에 축적된다. 그 결과 생물체의 세포막 구조가 변화되고 기능이 저해될 뿐더러 암과 돌연변이를 유발하고 $\ulcorner$환경호르몬$\lrcorner$으로서 생물체의 내분비계 기능을 교란하는 등 심각한 보건학적 그리고 환경생물학적 문제를 일으키고 있다. 염화 방향족화합물들은 벤젠고리 구조와 벤젠고리에 염소가 치환된 탄소-염소 결합을 공통적으로 가지고 있으며 벤젠고리에 치환된 염소의 수와 같은 수의 염소라도 붙어있는 위치에 따라 난분해 특징이 결정된다. 염화 방향족화합물들의 분해를 위해서는 미생물에 의한 벤젠 구조의 개환과정과 함께 벤젠 고리구조로부터 염소 치환기를 제거하는 탈염소화 과정이 반드시 일어나야만 한다. 호기적 환경에서 미생물에 의한 탈염소화는 분해 초기단계에서 dehalogenase라는 효소에 의해 촉매되는 oxygenolytic, reductive, 그리고 hydrolytic catalysis에 의해 일어나거나, 분해 대사과정 중에 저절로 염소치환기가 떨어져 나가는 경우도 있다. 탈염소화 과정을 거쳐 분해하는 미생물들을 이용한 염화 방향족 오염물질의 생물학적 분해방법은 이미 사용되고 있는 물리ㆍ화학적 방법보다 경제적이며 2차 오염의 부작용 없이 그 오염물질들을 매우 효과적으로 처리할 수 있다. 따라서 탈염소화 기작을 포함한 분해과정의 이해는 생물학적 분해의 기본적인 정보를 제공할 뿐더러 난분해성 환경 오염물질의 분해처리를 위하여 보다 집중적으로 연구해야 할 과제라고 할 것이다.

• 한국식품위생안전성학회지
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• 제22권1호
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• pp.1-14
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• 2007

The term, "food safety", has traditionally been viewed as a practical science aimed at assuring the prevention acute illnesses caused by biological microorganisms, and only to a minor extent, chronic diseases cause by chronic low level exposures to natural and synthetic chemicals or pollutants. "food safety" meant to prevent microbiological agents/toxins in/on foods, due to contamination any where from "farm to Fork", from causing acute health effects, especially to the young, immune-compromised, genetically-predisposed and elderly. However, today a broader view must also include the fact that diet, perse (nutrients, vitamins/minerals, calories), as well as low level toxins and pollutant or supplemented synthetic chemicals, can alter gene expressions of stem/progenitor/terminally-differentiated cells, leading to chronic inflammation and other mal-functions that could lead to diseases such as cancer, diabetes, atherogenesis and possibly reproductive and neurological disorders. Understanding of the mechanisms by which natural or synthetic chemical toxins/toxicants, in/on food, interact with the pathogenesis of acute and chronic diseases, should lead to a "systems" approach to "food safety". Clearly, the interactions of diet/food with the genetic background, gender, and developmental state of the individual, together with (a) interactions of other endogenous/exogenous chemicals/drugs; (b) the specific biology of the cells being affected; (c) the mechanisms by which the presence or absence of toxins/toxicants and nutrients work to cause toxicities; and (d) how those mechanisms affect the pathogenesis of acute and/or chronic diseases, must be integrated into a "system" approach. Mechanisms of how toxins/toxicants cause cellular toxicities, such as mutagenesis; cytotoxicity and altered gene expression, must take into account (a) irreversible or reversal changes caused by these toxins or toxicants; (b)concepts of thresholds or no-thresholds of action; and (c) concepts of differential effects on stem cells, progenitor cells and terminally differentiated cells in different organs. This brief Commentary tries to illustrate this complex interaction between what is on/in foods with one disease, namely cancer. Since the understanding of cancer, while still incomplete, can shed light on the multiple ways that toxins/toxicants, as well as dietary modulation of nutrients/vitamins/metals/ calories, can either enhance or reduce the risk to cancer. In particular, diets that alter the embryo-fetal micro-environment might dramatically alter disease formation later in life. In effect "food safety" can not be assessed without understanding how food could be 'toxic', or how that mechanism of toxicity interacts with the pathogenesis of any disease.

• 한국수자원학회논문집
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• 제53권6호
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• pp.395-408
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• 2020

본 연구논문은 북부아프리카의 알제리에 위치한 하천유역에서 다중선행일 유출량의 예측을 위하여 진화적 최적화기법과 k-fold 교차검증을 결합한 세 개의 서로 다른 기계학습 접근법 (인공신경망, 적응 뉴로퍼지 시스템, 그리고 웨이블릿 기반 신경망)을 개발하고 적용하는 것이다. 인공신경망과 적응 뉴로퍼지 시스템은 root mean squared error (RMSE), Nash-Sutcliffe efficiency (NSE), correlation coefficient (R), 그리고 peak flow criteria (PFC) 의 네 개의 통계지표를 기반으로 하여 모형의 훈련 및 테스팅 결과 유사한 모형수행결과를 나타내었다. 웨이블릿 기반 신경망모형은 하루선행일 테스팅의 결과 RMSE = 8.590 ㎥/sec 과 PFC = 0.252로 분석되어서 인공신경망의 RMSE = 19.120 ㎥/sec, PFC = 0.446 과 적응 뉴로퍼지 시스템의 RMSE = 18.520 ㎥/sec, PFC = 0.444 보다 양호한 결과를 나타내었고, NSE와 R의 값도 웨이블릿 기반 신경망모형이 우수한 것으로 나타났다. 그러므로 웨이블릿 기반 신경망은 알제리 세이보스 하천에서 다중선행일의 예측을 위하여 효율적인 도구로 사용할 수 있다.

• 한국농공학회논문집
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• 제59권1호
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• pp.45-55
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• 2017

Climate change causes changes in rainfall patterns, temperature and drought frequency. Climate change impact influences on water management and crop production. It is critical issue in agricultural industry. Rice is a staple cereal crop in South Korea and Korea uses a ponding system for its paddy fields which requires a significant amount of water. In addition, water supply has inter-relationship with crop production which indicates water productivity. Therefore, it is important to assess overall impacts of climate change on water resource and crop production. A water footprint concept is an indicator which shows relationship between water use and crop yield. In addition, it generally composed of three components depending on water resources: green, blue, grey water. This study analyzed the change trend of water footprint of paddy rice under the climate change. The downscaled climate data from HadGEM3-RA based on RCP 8.5 scenario was applied as future periods (2020s, 2050s, 2080s), and historical climate data was set to base line (1990s). Depending on agro-climatic zones, Suwon and Jeonju were selected for study area. A yield of paddy rice was simulated by using FAO-AquaCrop 5.0, which is a water-driven crop model. Model was calibrated by adjusting parameters and was validated by Mann-Whitney U test statistically. The means of water footprint were projected increase by 55 % (2020s), 51 % (2050s) and 48 % (2080s), respectively, from the baseline value of $767m^2/ton$ in Suwon. In case of Jeonju, total water footprint was projected to increase by 46 % (2020s), 45 % (2050s), 12 % (2080s), respectively, from the baseline value of $765m^2/ton$. The results are expected to be useful for paddy water management and operation of water supply system and apply in establishing long-term policies for agricultural water resources.

• The Korean Journal of Physiology and Pharmacology
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• 제17권3호
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• pp.223-228
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• 2013

The calcium-activated $K^+$ ($BK_{Ca}$) channel is one of the potassium-selective ion channels that are present in the nervous and vascular systems. $Ca^{2+}$ is the main regulator of $BK_{Ca}$ channel activation. The $BK_{Ca}$ channel contains two high affinity $Ca^{2+}$ binding sites, namely, regulators of $K^+$ conductance, RCK1 and the $Ca^{2+}$ bowl. Lysophosphatidic acid (LPA, 1-radyl-2-hydroxy-sn-glycero-3-phosphate) is one of the neurolipids. LPA affects diverse cellular functions on many cell types through G protein-coupled LPA receptor subtypes. The activation of LPA receptors induces transient elevation of intracellular $Ca^{2+}$ levels through diverse G proteins such as $G{\alpha}_{q/11}$, $G{\alpha}_i$, $G{\alpha}_{12/13}$, and $G{\alpha}s$ and the related signal transduction pathway. In the present study, we examined LPA effects on $BK_{Ca}$ channel activity expressed in Xenopus oocytes, which are known to endogenously express the LPA receptor. Treatment with LPA induced a large outward current in a reversible and concentration-dependent manner. However, repeated treatment with LPA induced a rapid desensitization, and the LPA receptor antagonist Ki16425 blocked LPA action. LPA-mediated $BK_{Ca}$ channel activation was also attenuated by the PLC inhibitor U-73122, $IP_3$ inhibitor 2-APB, $Ca^{2+}$ chelator BAPTA, or PKC inhibitor calphostin. In addition, mutations in RCK1 and RCK2 also attenuated LPA-mediated $BK_{Ca}$ channel activation. The present study indicates that LPA-mediated activation of the $BK_{Ca}$ channel is achieved through the PLC, $IP_3$, $Ca^{2+}$, and PKC pathway and that LPA-mediated activation of the $BK_{Ca}$ channel could be one of the biological effects of LPA in the nervous and vascular systems.

• 대한치과교정학회지
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• 제18권1호
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• pp.189-207
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• 1988

In almost all biologic systems, mechanically induced electric charge separation is a fundamental phenomenon. Since the hypothesis was established that the generation of electric potentials in bone by mechanical stress including muscular force might control the activity in bone by mechanical stress including muscular force might control the activity of osseous cells and their biopolymeric byproduct, the concept of electrically mediate growth mechanism, which involves biological growth and bone remodeling by any means, in living systems has been applied clinically and experimentally to orthopedic fracture repair, the regulation of orthodontic tooth movement, epiphyseal cartilage regeneration, etc. On the other hand, recent numerous research data available show apparently that the mandibular condyle has the characteristics of growth center as well as growth site. In addition, there exists a considerable difference of opinion as to the role of external pterygoid muscle in condylar growth. In view of these evidences, this. experiment was performed to investigate the effect of the galavic current on the growth of the mandible and condyle for elucidating the nature of condylar growth. The bimetallic device was composed of silver and platinum electrode connected with resistor (3.9 Mohm), which was expected to produce galvanic current of 23.6 nA according to the galvanic principle. The 25 Sprague-Dawley rats were divided into two group, 2 week group comprising 8 animals exposed to satanic current for 2 weeks and 3 control animals not exposed for 2 weeks, 4 week group comprising 10 animals in experimental group and 4 animals in control group applied for 4 weeks respectively. The experimental rats were subjected to application of the galvanic current invasively to codylar head surface and the control groups with sham electrode. On the basis of anatomic and histologic data from the mandibular condyle of experimental and control group, the following results were obtained. 1. After 2 weeks, there was no increase of mandibular size in experimental group over that of the control group. 2. After 4 weeks, the size of the condylar head was larger in experimental group than that of the control. 3. In 2 week group, the thickness of the mitotic compartment and hypertrophic chondroblastic layer was increased in experimental group. 4. In 4 week group, the number and the size of the hypertrophic chondroblasts were increased significantly on experimental group over that of the control group. 5. The application of the satanic current caused an increase in chondrocytic hypertrophy and intercellular matrix in both groups.