통합 검색 | Korea Science

Yoo, Kyung-Hyun;Lee, Tae-Young;Yang, Moon-Hee;Park, Eun-Young;Yook, Yeon-Joo;Lee, Hyo-Soo;Park, Jong-Hoon
- BMB Reports
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- 제41권8호
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- pp.593-596
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- 2008
ADPKD (Autosomal Dominant Polycystic Kidney Disease) is characterized by the progressive expansion of multiple cystic lesions in the kidneys. ADPKD is caused by mutations in Ed-pl. consider PKD1 and PKD2. Recently a relation between c-myc and the pathogenesis of ADPKD was reported. In addition, c-Myc is a downstream effector of PKD1. To identify the gene regulated by PKD2 and c-Myc, we performed gene expression profiling in PKD2 and c-Myc overexpressing cells using a human 8K cDNA microarray. NCAM (neuronal cell adhesion molecule) levels were significantly reduced in PKD2 overexpressing systems in vitro and in vivo. These results suggest that NCAM is an important molecule in the cystogenesis induced by PKD2 overexpession.
https://doi.org/10.5483/BMBRep.2008.41.8.593 인용 PDF

Lee, Yong-Sun;Lee, June;Kim, Hak-Kyun;Kang, Sukhyun;Han, Joo-Seok;Kim, Jae-Bum;Hwang, Deog-Su
- Animal cells and systems
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- 제7권1호
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- pp.81-87
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- 2003
While screening proteins that interact with DnaA protein, the initiator protein for Escherichia coil chromosomal DNA replication, we found a 52-kD sized protein which bound to DnaA protein in a salt-dependent manner. This protein was identified as trigger factor, a ribosome-associated peptidyl-prolyl- cisltrans isomerase with chaperone activity. Trigger factor was overproduced and purified to near homogeneity, and its effect on the function of DnaA protein was examined, Enhanced binding of DnaA protein to DnaA box with no apparent supershift in the gel-shift experiments suggested that trigger factor, by virtue of its chaperone activity, exerts a change on DnaA protein thus increasing its binding affinity for DnaA box.
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