• Title/Summary/Keyword: Biological sciences

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Cloning and Sequencing of the ${\beta}-Amylase$ Gene from Paenibacillus sp. and Its Expression in Saccharomyces cerevisiae

  • Jeong, Tae-Hee;Kim, Hee-Ok;Park, Jeong-Nam;Lee, Hye-Jin;Shin, Dong-Jun;Lee, Hwang-Hee Blaise;Chun, Soon-Bai;Bai, Suk
    • Journal of Microbiology and Biotechnology
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    • v.11 no.1
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    • pp.65-71
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    • 2001

  • A gene from Paenibacillus sp. KCTC 8848P encoding ${\beta}-amylase$ was cloned and expressed in Escherichia coli. The Paenibacillus ${\beta}-amylase$ gene cosisted of a 2,409-bp open reading frame without a translational stop codon, encoding a protein of 803 amino acids. The presumed ribosime-binding site, GGAGG, was located 10 bp upstream from the TTG initiation codon. The deduced amino acid sequence of the ${\beta}-amylase$ gene had a 95% similarity to the ${\beta}-amylase$ of Bacillus firmus. The ${\beta}-amylase$ gene was introduced into wild-type strains of Saccharomyces cerevisiae using a linearized yeast integrating vector containing a geneticin resistance gene and its product was secreted into the culture medium.

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