• Journal of Korean Society of Environmental Engineers
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• v.35 no.1
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• pp.1-9
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• 2013

Long term hydrogen production was investigated in an anaerobic sequencing batch reactor (ASBR) using mixed microflora. Glucose (about 8,250 mg/L) was used as a substrate for the ASBR operation under the condition of pH 5.5 and $37^{\circ}C$ with mixing at 150 rpm. The experiment was carried out over a period of 160 days. Hydrogen yield was 0.8mol $H_2/mol$ glucose with F/M ratio 2 at initial operation period. The hydrogen yield reached to maximum 2.6 mol $H_2/mol$ glucose at 80th day operation. However decreased hydrogen yield was observed after 80 days operation and eventually no hydrogen yield. Although well-known hydrogen producer Clostridium sp. was detected in the reactor by PCR-DGGE analysis, changed reactor operation was the major reason of the decreased hydrogen production, such as low F/M ratio of 0.5 and high propionic acid concentration 2,130 mg/L. Consequently the long period operation resulted in MLSS accumulation and then low F/M ration stimulating propionic acid formation which consumes hydrogen produced in the reactor.

• KSBB Journal
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• v.20 no.6
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• pp.393-400
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• 2005

Hydrogen($H_2$) as a clean, and renewable energy carrier will be served an important role in the future energy economy. Several biological $H_2$ production processes are known and currently under development, ranging from direct bio-photolysis of water by green algae, indirect bio-photolysis by cyanobacteria including the separated two stage photolysis using the combination of green algae and photosynthetic microorganisms or green algae alone, dark anaerobic fermentation by fermentative bacteria, photo-fermentation by purple bacteria, and water gas shift reaction by photosynthetic or fermentative bacteria. In this paper, biological $H_2$ production processes, that are being explored in fundamental and applied research, are reviewed.

• Transactions of the Korean hydrogen and new energy society
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• v.26 no.6
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• pp.507-515
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• 2015

HI decomposition reaction requires a catalyst for the efficient production of hydrogen as a key reaction for hydrogen production in sulfur-iodine thermochemical water-splitting (SI) cycle. As a catalyst used in the reaction, the performance of platinum catalyst is excellent. While, the platinum catalyst is not economical. Therefore, studies of a nickel catalyst that could replace platinum have been carried out. In this study, the characteristics of the catalytic HI decomposition on the amount of loaded nickel (Ni = 0.1, 0.5, 1, 3, 5, 10 wt%) were investigated. As the supported Ni amount increased up to 3 wt%, HI decomposition was found to increase in linear proportion. However, the conversion of $Ni/Al_2O_3$ catalyst loaded above 3 wt% was not linear. It was thought that the different HI decomposition characteristics was caused in the size and metal dispersion of Ni particles of catalyst. The physical property of catalyst before and after HI decomposition reaction was characterized by BET, chemisorption, XRD and SEM analysis.

• Journal of the Korean Ceramic Society
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• v.50 no.6
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• pp.485-488
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• 2013

A SiC-$CeO_2$ composite membrane was successfully fabricated using an ally-hydridopolycarbosilane (AHPCS) binder and treated by dip-coating at 60 times with a $CeO_2$ sol solution. The dip-coated SiC membrane was calcined at 773 K and then sintered at 1173 K under an air atmosphere. The coated membrane was characterized by X-ray diffraction (XRD), field-emission scanning electron microscopy (FE-SEM) and a BET surface analysis. The difference in permeation performance between $H_2$ and CO gases was measured by varying the temperature. The permeation flux of $H_2$ on the SiC membrane with layered $CeO_2$ was obtained as $8.45{\times}10^{-6}\;mol/m^2sPa$ at room temperature. The CO permeation flux was $2.64{\times}10^{-6}\;mol/m^2sPa$ at room temperature. The reaction enthalpy (${\Delta}H^{\circ}$) for the hydrogen permeation process was calculated as -7.82 J/mol by Arrhenius plots.

• Journal of Environmental Science International
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• v.5 no.1
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• pp.51-59
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• 1996

The purpose of this study was to evaluate the partial oxidation of the biological treatment plant effluents using Fenton's reagent as a pretreatment step prior to a tertiary biological oxidation of these effluents. Fenton's reagent was evaluated as a pretreatment process for inhibitory or refractory organics. Based on the Fenton oxidation system, the petrochemical wastewater treatment plant effluent was shown to have significant improvement in toxicity after oxidation with hydrogen peroxide. For example, at ranee of 42 ∼ 184 mg/L COD of petrochemical plant effluents, the COD removal efficiencies were from 38.2% to 60.1% after reaction with hydrogen peroxide 200 mg/L and Fe2+ 100 mg/L and reaction time was 30 minutes. The total TOC reduction were about 15.8∼22.4% with same test condition and difference between the overall removal rate and BOD/COD ratio after Fenton's oxidation estabilished in the biodegradation and otherwise meets the discharge standard or reuse for cooling tower make-up water.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.08a
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• pp.272-272
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• 2013

The attractive features of photosynthetic reaction center proteins for energy application make them useful in solar energy conversion to hydrogen fuel or electrical energy. Almost unity charge separation quantum yield and its rapid speed of ~1ns, absorbance region in visible light (480~740 nm) and high proportion of photosynthetically active solar energy of 48.5% allowed photosystem1 to exploited as a bio-material for photo-energy devices. Directionality of photosystem1 in electron transfer can solve main problem in two-step water splitting process where back reaction deteriorates the overall efficiency. In the study, photosystem1 was extracted from spinach and the photo-induced excited electron in the reaction center was utilized in various field of light energy application. First, hydrogen evolving system realized by photodeposition of platinum at the end of the electron transfer chain, with combining specific semiconductor to oxidize water in the first step of Z-scheme. The evaluation by gas-chromatography demonstrated hydrogen evolution through the system. For the further application of photoelectrical material on electrode, photosystem1 have been controlled by copper ion, which is expected to assemble photosystem in specific orientation followed by maximized photoelectrical ability of film. The research proposed concrete methods for combining natural protein and artificial materials in one system and suggested possibility of designing interface between biological and inorganic materials.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.162-163
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• 2003

Through the experiment, process of producing hydrogen and organic compounds using C. reinhardtii has been researched. In dark fermentation, pH and dry cell weight as well as starch content decreased as time goes, while organic compounds and hydrogen were produced more in accordance with time. Still, organic compounds showed tendency to increase in accordance with time, but hydrogen reached the maximum on the third day.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.1
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• pp.144-154
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• 2008

The rumen microbial ecosystem produces methane as a result of anaerobic fermentation. Methanogenesis in the rumen is thought to represent a 2-12% loss of energy intake and is estimated to be about 15% of total atmospheric methane emissions. While methanogenesis in the rumen is conducted by methanogens, PCR-based techniques have recently detected many uncultured methanogens which have a broader phylogenetic range than cultured strains isolated from the rumen. Strategies for reduction of methane emissions from the rumen have been proposed. These include 1) control of components in feed, 2) application of feed additives and 3) biological control of rumen fermentation. In any case, although it could be possible that repression of hydrogen-producing reactions leads to abatement of methane production, repression of hydrogen-producing reactions means repression of the activity of rumen fermentation and leads to restrained digestibility of carbohydrates and suppression of microbial growth. Thus, in order to reduce the flow of hydrogen into methane production, hydrogen should be diverted into propionate production via lactate or fumarate.

• Horticultural Science & Technology
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• v.33 no.3
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• pp.383-389
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• 2015

Chitooligosaccharide (COS), as antioxidant, extensively applied to food and juice preservation. In the present study, influences of COS on vase life and ornamental value of cut roses were investigated. Results showed that vase life of cut roses treated by COS was longer 6.4 days than one of control and ornamental character of cut roses was improved effectively by COS. The increase of vase life and ornamental value were chiefly governed by that COS improved water absorption capacity of cut roses. Besides that, COS decreased the contents of superoxide anion and hydrogen peroxide and lowered the levels of malondialdehyde in turn during the senescence process of cut roses. That was because that COS not only enhanced activities of antioxidant enzymes glutathione reductase, but also improved reduced glutathione contents in petals of cut rose. Therefore, COS could be used in commercial preservatives to improve the longevity of cut roses.

• Proceedings of the KOSOMBE Conference
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• v.1998 no.11
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• pp.72-73
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• 1998

A new method for enzyme immobilization has been developed to remove interference by potential interferents in body fluids. Instead of using electron mediators, we chose direct hydrogen peroxide measurement route. Extremely hydrogen peroxide-selective polymer was coated as an inner membrane to exclude interferents and then glucose oxidase(GOx) was entrapped by electropolymerization of inert monomers. There was no solvent casting step throughout the whole fabrication procedure but all membranes on Pt-Ir electrode were formed by electropolymerization. Thus, membrane thickness, quantity of enzyme loaded and can be controlled by electrochemical parameters. As a result, reproducibility of biosensor characteristics becomes remarkably improved in terms of mass production.