• 한국물환경학회지
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• 제32권3호
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• pp.303-309
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• 2016

This study aimed to assay the biological removal of TDS (total dissolved solids) from RO (reverse osmosis) rejected water. Following bio-sorption of TDS with AGS (aerobic granular sludge), the effects of TDS on biological nitrogen removal were examined. The bio-sorption of TDS after AGS treatment was confirmed by checking for TDS removal efficiency and surface analysis of microorganisms with SEM and EDS. Then, the effects of TDS on biological nitrogen removal and the denitrification efficiency were evaluated using the MBR reactor. According to the results, the bio-sorption of TDS with AGS was 0.1 mg TDS/mg AGS, and we confirmed that the microorganism surfaces had adsorbed the TDS. Biological nitrogen removal efficiency was measured at inhibiting denitrification at 4,000 mg/L of TDS-injected material. Based on this study, it is necessary to pretreat TDS-containing RO rejected water and to maintain TDS concentration lower than a specific value (≤4,000 mg/L), when considering biological nitrogen removal.

• 한국물환경학회지
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• 제18권3호
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• pp.237-243
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• 2002

The effect of a salinity on the performance of a biological nutrient removal system was investigated using a model SBR(Sequencing Bach Reactor) system. The system was operated at a 12hr, 18hr, 24hr, and 36hr HRT with a salinity level of 20,000mg/L and compared with a system similarly operated with fresh water. The influent salinity level of 8,000 mg/L does not have a significant effect on BOD removal efficiency, there is a noticeable decrease in BOD removal rate from 10,000 mg Cl-/L. The Nitrogen could be removed from the saline wastewater with the same efficiency as for the fresh water because of low C/N ratio in anoxic period. The excess biological phosphorous removal is highly affected by the increase in the influent salinity. The efficiency is decreased from 96.6% to 43.4% when the influent salinity is increased from 0 to 20,000mg/L.

• 상하수도학회지
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• 제20권1호
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• pp.44-52
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• 2006

Bio-filtration processes using honeycomb tubes (process 1) and aeration and manganese-sand filtration (process 2) were evaluated for the biological manganese removal efficiency. The concentration of manganese at effluent was stabilized after 20days operation in process 1. It was estimated the required time for attaching and growing microorganisms to honeycomb tubes. In long term of operation periods, manganese removal efficiency was dropped for the excessively attached biofilm and manganese dioxide to honeycomb tubes. It took several days for normal operation in process 2, after that manganese removal efficiency was increased to 98% and stabilized for 1.5 years. Microorganisms in process 1 and 2 were isolated and cultured to characterize manganese-oxidizing bacteria. Among the four types of colony, light brown colony was turned blue color by leuco crystal violet spot test. Stenotropomonas genus, known as manganese-oxidizing bacteria, was identified by 16S rDNA partial sequencing analysis which was isolated in process 1 and 2. For the biological treatment to remove manganese, these two considerations are important. One is to choose the proper media attaching manganese oxidant, another one is to define the cultural condition of isolated manganese-oxidizing bacteria.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권4호
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• pp.288-292
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• 2006

Through statistically designed experiments, lysis agents were optimized to effectively disrupt bacterial cells in a microfluidic device. Most surfactants caused the efficient lysis of Gram-positive microbes, but not of Gram-negative bacteria. A Plackett-Burman design was used to select the components that increase the efficiency of the lysis of the Gram-negative bacteria Escherichia coli. Using this experimental design, both lysozyme and benzalkonium chloride were shown to significantly increase the cell lysis efficiency, and ATP was extracted in proportion to the lysis efficiency. Benzalkonium chloride affected the cell membrane physically, while lysozyme destroyed the cell wall, and the amount of ATP extracted increased through the synergistic interaction of these two components. The two-factor response-surface design method was used to determine the optimum concentrations of lysozyme and benzalkonium chloride, which were found to be 202 and 99 ppm, respectively. The lysis effect was further verified by microscopic observations in the microchannels. These results indicate that Gram-negative cells can be lysed efficiently in a microfluidic device, thereby allowing the rapid detection of bacterial cells using a bioluminescence-based assay of the released ATP.

• Biotechnology and Bioprocess Engineering:BBE
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• 제6권4호
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• pp.269-273
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• 2001

This study presents a new formulation method for improving DNA transfection effi-ciency using a fusogenic peptide and polyethylene glycol-grafted polyethylenimine. Succinimidyls succinate polyethylene glycol (PEG-SSA) was conjugated with polyethylenimine(PEL). PEL is well known for a good endosomal escaping and DNA condensign agent. The positively charged syn-thetic fusogenic peptide, KALA was coated on the negatively charged PEG-g-PEI/DNA and PEI/DNA complexes. The KALA/PEI/ DNA complexes exhibited aggregation behavior at higher KALA coating amount with an effective diameter of around 1,000 nm. However, the LALA/PEG-g-PEI/DNA complexes were 100-300 nm in size with a surface zeta-potential (ζ)value of about +20mV. The conjugated PEG molecules suppressed any KALA-mediated inter-particle aggregation, and thereby improved the transfection efficiency, Consequently, the transfection efficiency of the KALA/PEG-g-PEI/DNA complexes was obtained by utilizing both the fusogenic activity of KALA and the steric repulsion effect of PEC.

• Mycobiology
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• 제38권3호
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• pp.184-188
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• 2010

Calocybe indica, known as milky white mushroom, grows and cultivated in the sub-tropical and temperate zones of South Asia. We investigated the most suitable supplements and their levels for the commercial cultivation of milky white mushroom. Rice bran, maize powder, and wheat bran with their different levels (10, 20, 30, 40, and 50%) were used as supplements to evaluate the yield and yield contributing characteristics of C. indica. Primordia initiation was observed between 13.5 and 19.3 days. The results indicated that the 30% maize powder supplement was effective for producing viable fruiting bodies. The maximum diameters of the pileus and stalk were observed with 30% maize powder. The highest biological and economic yield and biological efficiency were also obtained with 30% maize powder as a supplement. The results indicate that increasing the supplement level resulted in less biological efficiency, and that 30% maize powder was the best supplement level for rice straw substrate to cultivate milky white mushrooms.

• 상하수도학회지
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• 제18권5호
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• pp.649-655
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• 2004

The behavior of biological particles in DAF (dissolved air flotation) process was analyzed by employing PBT (Population balance theory). After decline growth phase of activated sludge, the value of the initial collision-attachment efficiency was increased over than 0.35 corresponding relatively high value in the whole life cycle of microorganism. For practical application of DAF as a solid separation process. It is desirable that microbial particles should be operated to perform high solid removal efficiency in biological wastewater treatment.

• 한국태양에너지학회 논문집
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• 제28권2호
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• pp.34-41
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• 2008

A low-temperature solar thermal system assisting a biological nitrogen treatment reservoir was designed and field-tested. A large tank whose temperature was maintained at about $25-30^{\circ}C$ to enhance the performance of a biological nitrogen treatment process was heated by an array of flat plate solar collectors. Test results revealed that the overall collector efficiency was above 50% for the most cases tested. This high efficiency was possible owing to the relatively low collector temperature that can be traced back to the reservoir temperature. A substantial enhancement in nitrogen treatment was observed as a result of maintaining the reservoir temperature higher.

• 상하수도학회지
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• 제20권6호
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• pp.837-848
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• 2006

This paper presents the test results of biological aerated filtration(BAF) process to replace activated sludge process by enhancing treated effluent quality and reducing the costs. In BAF process both BOD and SS compounds in wastewater are degraded and removed by biological reaction and filtration. Upflow BAF with expanded polypropylene media and downflow BAF with ceramic media were used to investigate the effects of hydraulic and organic loads on effluent quality. As a result, in BAF processes which has different media, upflow BAF reactor shows 5% higher efficiency than downflow BAF and this phenomena caused by backwashing methods and operational conditions. The results of influence factors analyzed by Factor Analysis Method in BOD and SS treatment efficiency are the size of media, hight of media bed and type of media. The quantitative effects of media size are 5.73% in TBOD, 5.78% in SBOD and 7.65% in TSS, so we confirmed the main factor is media size.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2006년도 춘계학술대회
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• pp.559-562
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• 2006

A low-temperature solar thermal system assisting a biological nitrogen treatment reservoir was designed and field-tested. A large tank whose temperature was maintained at about $25{\sim}30^{\circ}C$ to enhance the performance of a biological nitrogen treatment process was heated by an array of flat plate solar collectors. Test results revealed that the overall collector efficiency was above 50% for the most cases tested. This high efficiency was possible owing to the relatively low collector temperature that can be traced back to the reservoir temperature. A substantial enhancement in nitrogen treatment was observed as a result of maintaining the reservoir temperature higher.