• 한국재료학회:학술대회논문집
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• 한국재료학회 2008년도 춘계학술발표대회 및 제14회 신소재 심포지엄
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• pp.6-6
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• 2008

• 한국동물학회:학술대회논문집
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• 한국동물학회 2003년도 한국생물과학협회 학술발표대회
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• pp.184.4-184
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• 2003

No Abstract, See Full Text

• 한국해양학회지:바다
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• 제12권2호
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• pp.112-120
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• 2007

본 총설에서는 연어과 어류의 계군 분석을 위한 생물학적 표식으로서의 기생충의 유용성에 관하여 다루었다. 계군의 정의는 학자에 따라 다양하지만, 대부분은 본질적으로 서로 유사한 생물학적 특징을 가지며 타 계군과의 혼합 없이도 스스로 번식이 가능하여 일정한 규모를 유지할 수 있는 일련의 개체들의 모임을 계군으로 정의하고 있다. 이 계군을 관리하는 일은 지속적인 생산 및 소비를 위하여 매우 중요하며 특히 연어과 어류의 계군은 각국이 지속적인 자원 확보를 위하여 적극적으로 치어를 방류하고 있으며, 이는 각국의 자산으로 간주되므로 공해 상에서 계군을 구분하여야 한다. 계군을 구분하는 방법은 매우 다양하다. 인공 표식, 기생충과 같은 생물학적 표식, 이석 분석, 비늘 분석, 유전정보 분석 등의 방법이 있는데, 각각 장점과 단점이 있으며, 이 중에서 기생충과 같은 생물학적 표식은 별도의 비용이 들지 않는다는 장점이 있다. 기생충이 존재하는 수역을 감수성이 있는 어류가 통과할 때 이 기생충에 감염이 된다. 이후, 감염된 어류가 이동하여 기생충이 존재하지 않는 수역에서 포획될 경우, 이 개체가 기생충이 존재하는 지역을 통과하였음을 유추해 낼 수 있다. 따라서 이 개체는 기생충에 의해 자연히 표식되는 셈이 된다. 그러나 이 지역을 통과하지 않는 개체는 기생충에 의해 표식되지 않는다. 그러므로 이 생물학적 표식을 통해 각각의 계군을 구분할 수 있으며 이동 경로도 추적이 가능하다. 여기서는 연어과 어류 연구를 목적으로 기생충을 생물학적 표식으로 사용한 각종 예를 들었으며, 이 방법의 장점 및 단점 또한 서술하였다. 연어(Oncorhyunchus keta)는 국내에 소상하는 주된 연어과 어류이며, 북태평양 전역에 분포한다. 한국산 연어는 오호츠크해를 거쳐 북서태평양 및 베링해로 이동한 후 회유하는 것으로 생각된다. 그렇지만, 한국산 연어의 공해 상에서 분포 및 회유 경로에 대해서는 확실하게 알려지지 않은 부분이 많으며 한국산 연어 계군을 타 계군과 확실하게 구분할 수 있는 표식도 아직까지는 존재하지 않는다. 여기에서는 기생충에 관한 정보를 포함한 한국산 연어의 계군 분석에 대한 최근의 연구 결과에 관하여 마지막으로 언급하였다.

• 한국환경과학회지
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• 제23권8호
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• pp.1495-1505
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• 2014

The objective of this study was to evaluate survival rate and fish movement (migration) using a tagging approach of passive integrated transponder (PIT) in Juksan Weir, which was constructed as a four major river restoration projects. For this study, survival rates of each fish species and the mobility of fish individuals were analyzed during 2 weeks by the insertion of PIT tags to various fish species in the laboratory. According to tagging tests in the laboratory, the survival rate 37.5% (30 survivals of 80 individuals) after the insertion of PIT tags. The survival rate of Carassius auratus and Hemibarbus labeo was 100% and 80% after the insertion of the tags, respectively, whereas it was only 13.3% for Zacco platypus. In the field experiments of Juksan Weir, 6 species and 157 individuals from 8 species (563 individuals) were detected in the fixed automatic data-logging system, indicating a detection rate of 27.9% in the fishway of Juksan Weir. In the meantime, some species with no or low detection rates in the fixed automatic data-logging system were turn out to be stagnant-type species, which prefer stagnant or standing water to live.

• Archives of Pharmacal Research
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• 제27권4호
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• pp.422-428
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• 2004

Expressed sequence tags (ESTs) were generated from two 3'-directed CDNA libraries constructed from quiescent and activated rat hepatic stellate cell (HSC) to analyze the expression profiles of active genes in both cells. From quiescent and activated HSC, 694 ESTs and 779 ESTs, respectively, were obtained after excluding those having shorter than 30 bp. Amonq ESTs obtained from quiescent and activated HSC, 68 and 73 kinds of ESTs (186 clones and 236 clones), respectively, appeared more than once, implying that their genes are expressed highly in each cell type. 52 among 73 ESTs appeared only in the activated HSC 47 amonq 68 ESTs only in the normal HSC, and 21 in both cells. The genes of these 52 ESTs were assumed to be expressed more highly in the activated HSC. To confirm the high expression of genes of which the ESTs appeared more than twice in the activated HSC, northern hybridization was carried out with RNAs derived from rat normal and fibrotic liver using each of 18 EST DNAs as probe. 13 ESTs showed more intense bands with RNA isolated from the fibrotic liver than normal liver. From these results, we confirm the positive correlation between abundance of transcript in activated HSCs and the expression level in fibrotic liver, The expression profile of the transcripts serves as an important tool in understanding the biological properties of HSC.

• Parasites, Hosts and Diseases
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• 제39권2호
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• pp.151-160
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• 2001

Randomly selected 435 clones from Acanthamoeba healyi cDNA library were sequenced and a total of 387 expressed sequence tags (ESTs) had been generated. Based on the results of BLAST search, 130 clones (34.4%) were identified as the genes enconding surface Proteins , enzymes for DNA, energy Production or other metabolism, kinases and phosphatases, protease, proteins for signal transduction, structural and cytoskeletal proteins, cell cycle related proteins, transcription factors, transcription and translational machineries, and transporter proteins. Most of the genes (88.5%) are newly identified in the genus Acanthamoeba. Although 15 clones matched the genes of Acanthamoeba located in the public databases, twelve clones were actin gene which was the most frequently expressed gene in this study. These ESTs of Acanthamoeba would give valuable information to study the organism as a model system for biological investigations such as cytoskeleton or cell movement, signal transduction, transcriptional and translational regulations. These results would also provide clues to elucidate factors for pathogenesis in human granulomatous amoebic encephalitis or keratitis by Acanthamoeba.

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2006년도 제47회 학술심포지움 및 추계국제학술대회
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• pp.44.1-44.1
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• 2006

• Journal of Microbiology and Biotechnology
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• 제15권5호
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• pp.1060-1066
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• 2005

Under environmental stress, such as strong irradiance or nitrogen deficiency, unicellular green algae of the genus Haematococcus accumulate secondary carotenoids, i.e. astaxanthin, in the cytosol. The induction and regulation of astaxanthin biosynthesis in microalgae has recently received considerable attention owing to the increasing use of secondary carotenoids as a source of pigmentation for fish aquacultures, and as a potential drug in cancer prevention as a free-radical quencher. Accordingly, this study generated expressed sequence tags (ESTs) from a library constructed from astaxanthin-induced Haematococcus pluvialis. Partial sequences were obtained from the 5' ends of 1,858 individual cDNAs, and then grouped into 1,025 non-overlapping sequences, among which 708 sequences were singletons, while the remainder fell into 317 clusters. Approximately $63\%$ of the EST sequences showed similarity to previously described sequences in public databases. H. pluvialis was found to consist of a relatively high percentage of genes involved in genetic information processing ($15\%$) and metabolism ($11\%$), whereas a relatively low percentage of sequences was involved in the signal transduction ($3\%$), structure ($2\%$), and environmental information process ($3\%$). In addition, a relatively large fraction of H. pluvialis sequences was classified as genes involved in photosynthesis ($9\%$) and cellular process ($9\%$). Based on this EST analysis, the full-length cDNA sequence for superoxide dismutase (SOD) of H. pluvialis was cloned, and the expression of this gene was investigated. The abundance of SOD changed substantially in response to different culture conditions, indicating the possible regulation of this gene in H. pluvialis.

• 대한수의학회지
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• 제39권4호
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• pp.797-805
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• 1999

T sergenti cDNA library were constructed to get a more broad information about the structural, functional or antigenic properties of the proteins, and analyzes for their partial cDNA sequences and expression sequences tags(ESTg). The mRNA were purified from T sergenti isolates to identify the information of antigen gene, then first and second strand cDNA was synthesized. EcoR I adaptor ligation and Xho I enzyme restriction were used to the synthesized cDNA, and ligated into a Uni-ZAP XR vector. T sergenti cDNA library was constructed with packaging and amplification in vitro. Antibody screening was performed with constructed T sergenti cDNA library using antisera against T sergenti. Among those clones, eight phagemids were rescued from the recombinant in vivo excision with f1 helper phage. Using the analysis of endonuclease restriction and PCR, the recombinant cDNA were proved having a 0.5-3.0kb of inserts. The eight of partial cDNA clones' sequences were obtained and examined for their homology using BLASTN and BLASTX. The eight of sequenced clones were classified into three groups according to the basis of database searches. A total 3,045bp of partial cDNA sequence were determined from six clones. The putatively identified clones contain a cytochrome c gene, a heat shock protein gene, a cyclophilin gene, and a ribosomal protein gene. The unidentified clones have a homology to ATP-binding protein(mtrA) gene of S argillaceus, DNA-binding protein(DBP) gene of Pseudorabies virus 85kDa merozoite protein gene of B bovis, mRNA spm1 protein of T annulata and glycine-rich RNA-binding protein mRNA of O sativa etc.

• 대한의용생체공학회:의공학회지
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• 제28권3호
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• pp.325-331
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• 2007

Personal identification is essential for the automatic measurement of biosignal information in home healthcare systems. Personal identification is usually achieved with passive radio frequency identification (RFID), which does little more than store a unique identification number. However, passive RFID is not ideal for automatic identification. We present a user identification system based on radio signal strength indication (RSSI) using ZigBee for active RFID tags. Personal identification is achieved by finding the largest RSSI value from aggregated beacon messages that are periodically transmitted by active RFID tags carried by users. Obtaining reliable person!'.! identification without restricting the orientation requires a certain distance between the closest active RFID tag from the ZED and the second closest tag. The results show that the closest active RFID tag from the ZED and the second closest tag must be at least 70 cm apart to achieve reliable personal identification.