• Title/Summary/Keyword: Biological Engineering

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Ground-based Remote Sensing Technology for Precision Farming - Calibration of Image-based Data to Reflectance -

  • Shin B.S.;Zhang Q.;Han S.;Noh H.K.
    • Agricultural and Biosystems Engineering
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    • v.6 no.1
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    • pp.1-7
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    • 2005
  • Assessing health condition of crop in the field is one of core operation in precision fanning. A sensing system was proposed to remotely detect the crop health condition in terms of SP AD readings directly related to chlorophyll contents of crop using a multispectral camera equipped on ground-based platform. Since the image taken by a camera was sensitive to changes in ambient light intensity, it was needed to convert gray scale image data into reflectance, an index to indicate the reflection characteristics of target crop. A reference reflectance panel consisting of four pieces of sub-panels with different reflectance was developed for a dynamic calibration, by which a calibration equation was updated for every crop image captured by the camera. The system performance was evaluated in a field by investigating the relationship between com canopy reflectance and SP AD values. The validation tests revealed that the com canopy reflectance induced from Green band in the multispectral camera had the most significant correlation with SPAD values $(r^2=0.75)$ and NIR band could be used to filter out unwanted non-crop features such as soil background and empty space in a crop canopy. This research confirmed that it was technically feasible to develop a ground-based remote sensing system for assessing crop health condition.

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Nitrogen Removal from a mixed Industrial Wastewater using Food-Waste Leachate and Sugar Liquid Waste as External Carbon Sources: Full-Scale Experiment (혼합 산업폐수의 질소제거를 위한 외부 탄소원 투입과 물질수지: 실증실험)

  • Lee, Monghak;Ahn, Johwan;Lee, Junghun;Bae, Wookeun;Shim, Hojae
    • Journal of Korean Society on Water Environment
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    • v.28 no.5
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    • pp.663-668
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    • 2012
  • The feasibility of enhancing biological nutrient removal from an industrial wastewater was tested with food waste leachate and sugar liquid waste as external carbon sources. Long term influences of adding external carbon sources were investigated to see how the biological nutrient removal process worked in terms of the removal efficiency. The addition of the external carbons led to a significant improvement in the removal efficiency of nutrients: from 49% to approximately 76% for nitrogen and from 64% to around 80% for phosphorus. Approximately, 20% of the removal nitrogen was synthesized into biomass, while the remaining 80% was denitrified. Though the addition of external carbon sources improved nutrient removal, it also increased the waste sludge production substantially. The optimal observed BOD/TN ratio, based on nitrogen removal and sludge production, was around 4.0 in this study.

Electrochemical Properties of LiCoO2 Prepared by Mechanochemical Process (Mechanochemical Process로 제조된 LiCoO2의 전기화학적 특성)

  • Cho, Byung-Won;Lee, Joong Kee;Lee, Jae-Ryong;Kim, Su-Jin;Lee, Kwan-Young;Na, Byung-Ki
    • Korean Chemical Engineering Research
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    • v.46 no.1
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    • pp.69-75
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    • 2008
  • Discharge capacity of $LiCoO_2$ in preparation by mechanochemical process decreased remarkably over 4.3V. However, Zr coating of $LiCoO_2$ showed very stable electrochemical properties up to 4.5V. Zr coating of $LiCoO_2$ in this experiment showed the discharge capacity of 197 mAh/g at 3.0-4.5V, and it maintained 96% of the initial discharge capacity after 50 cycle of charge/discharge.

Characteristics of Lignin Removal in Cellulosic Ethanol Production Process (셀룰로오스 에탄올 생산공정에서 리그닌의 제거특성)

  • Lee, You-Na;Lee, Seung-Bum;Lee, Jae-Dong
    • Applied Chemistry for Engineering
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    • v.22 no.1
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    • pp.77-80
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    • 2011
  • In this study, we measured changes in the lignin content of acidified lignocellulosic biomass such as rice straw, saw dust, chestnut shell and peanut hull and analyzed the conversion property to cellulosic ethanol. It turns out that the lignin content increases in chestnut shell, rice straw, saw dust, peanut hull order and the conversion property to cellulosic ethanol is superior in the reverse order. Thus, the removal of lignin by acidification is necessary. In addition, as the concentration of sulfuric acid increases, the lignin content decreases and the yield of cellulosic ethanol increased. The optimum concentration of sulfuric acid is 20 wt%.

In vitro biological activities of lotus (Nelumbo nucifera) leaves extract (연잎(Nelumbo nucifera) 추출물의 in vitro 생리활성)

  • Park, Eun Jung;Cho, Hye Won;Park, Yu Jin;In, Man-Jin;Kim, Dong Chung
    • Journal of Applied Biological Chemistry
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    • v.64 no.2
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    • pp.121-125
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    • 2021
  • Antioxidative, anticoagulant, and α-glucosidase inhibitory effects of 60% (v/v) ethanolic extract from lotus (Nelumbo nucifera) leaves were investigated in vitro. The yield and polyphenol content of the lotus leaves extract were 35.3±1.1% and 186.2±5.2 ㎍ gallic acid equivalents/mg, respectively. The lotus leaves extract effectively scavenged free and cation radicals, and nitrite in a concentration-dependent manner, and possessed potent reducing power. The lotus leaves extract had remarkable anticoagulant and α-glucosidase inhibitory activities as a function of the extract concentration.

Electrochemical Properties of LiMPO4(M = Fe, Mn) Synthesized by Sol-Gel Method (졸-겔법에 의해 제조된 LiMPO4(M = Fe, Mn) 양극 활물질의 전기화학적 특성)

  • Kim, Jae-Kwang;Baek, Dong-Ho;Shin, Yong-Jo;Ahn, Jou-Hyeon;Seo, Yang-Gon;Kim, Chi-Su;Yoon, Seok-Jun;Cho, Myung-Hun
    • Journal of the Korean Electrochemical Society
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    • v.11 no.2
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    • pp.120-124
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    • 2008
  • Carbon-coated $LiFePO_4$ and $LiMn_{0.4}Fe_{0.6}PO_4$ cathode materials for lithium batteries were synthesized by a sol-gel method. X-ray diffraction and scanning electron microscopy data showed that the cathode materials are pure crystalline and are surrounded by porous carbon. The initial discharge capacities of $LiFePO_4$ and $LiMn_{0.4}Fe_{0.6}PO_4$ with the liquid electrolyte of 1M $LiPF_6$ in EC/DMC are 132 mAh/g and 145 mAh/g, respectively, at current density of 0.1 C-rate. $LiFePO_4$ and $LiMn_{0.4}Fe_{0.6}PO_4$ with an electrospun polymer-based electrolyte exhibit initial discharge capacities of 114 and 130 mAh/g at 0.1 C-rate at room temperature, respectively.

Cloning of a $\Delta5$ desaturase from Thraustochytrium sp. 26185 and Functional Expression in Pichia Pastoris (Thraustochytrium sp. 26185 균주에서의 $\Delta5$ desaturase 유전자 클로닝 및 Pichia pastoris 내에서의 기능적 발현)

  • Chung Tae-Ho;Lee Su-Jin;Oh Hyo-Jeong;Kim Geun-Joong;Hur Byung-Ki
    • KSBB Journal
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    • v.20 no.2 s.91
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    • pp.93-99
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    • 2005
  • Polyunsaturated fatty acids, that is PUFAs, are important constituents of membranes particularly found in the retina and central nervous system. In microorganism-based PUFAs biosynthesis, the genus Thraustochytrids is well evaluated for their potential as a promising candidate in the practical production of PUFAs, such as AA and DHA. In this study, we attempted to optimize a method of total nucleic acid extraction from this microorganism as a preliminary experiment. Using the extracted nucleic acid and degenerated primers for direct PCR, we isolated a $\Delta5$ desaturase gene that contained 1320-nucleotide and encoded 439 amino acids. This gene exhibited an expected function, when expressed in P. pastoris in the presence of appropriate exogenous substrate, as an evidence for $\Delta5$ desaturase activity (conversion of DGLA to AA). These results and information could provide a basis for the construction of engineered strains suitable for the practical production of PUFAs.

Copying and Manipulating Nature: Innovation for Textile Materials

  • Rossbach, Volker;Patanathabutr, Pajaera;Wichitwechkarn, Jesdawan
    • Fibers and Polymers
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    • v.4 no.1
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    • pp.8-14
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    • 2003
  • This paper considers the potential impact of biological approaches such as bio-copying (biomimetics) and biomanipulating (e.g. genetic engineering) on future developments in the field of textiles and, in particular, fibres. If analytical tools for studying biological systems combined with those of materials science are further developed, and higher efficiency and reproducibility of genetic engineering technology can be achieved, the potential for the copying and manipulation of nature for textile innovations will be immense. The present state for both fields is described with examples such as touch and close fastener, structurally coloured fibres, the Lotus of lect (for bio-copying), as well as herbicide tolerant cotton, insecticide resistant cotton (Bt cotton), cotton polyester bicomponent fibres, genetically engineered silkworm and silk protein, and spider fibres. (for genetic engineering).

Five Most Common Prognostically Important Fusion Oncogenes are Detected in the Majority of Pakistani Pediatric Acute Lymphoblastic Leukemia Patients and are Strongly Associated with Disease Biology and Treatment Outcome

  • Awan, Tashfeen;Iqbal, Zafar;Aleem, Aamer;Sabir, Noreen;Absar, Muhammad;Rasool, Mahmood;Tahir, Ammara H.;Basit, Sulman;Khalid, Ahmad Mukhtar;Sabar, Muhammad Farooq;Asad, Sultan;Ali, Agha Shabbir;Mahmood, Amer;Akram, Muhammad;Saeed, Tariq;Saleem, Arsalan;Mohsin, Danish;Shah, Ijaz Hussain;Khalid, Muhammad;Asif, Muhammad;Haq, Riazul;Iqbal, Mudassar;Akhtar, Tanveer
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.11
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    • pp.5469-5475
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    • 2012
  • Background and Objectives: Acute lymphoblastic leukemia (ALL) is a complex genetic disease involving many fusion oncogenes (FO) having prognostic significance. The frequency of various FO can vary in different ethnic groups, with important implications for prognosis, drug selection and treatment outcome. Method: We studied fusion oncogenes in 101 pediatric ALL patients using interphase FISH and RT-PCR, and their associations with clinical features and treatment outcome. Results: Five most common fusion genes i.e. BCR-ABL t (22; 9), TCF3-PBX1 (t 1; 19), ETV6-RUNX1 (t 12; 21), MLL-AF4 (t 4; 11) and SIL-TAL1 (del 1p32) were found in 89/101 (88.1%) patients. Frequency of BCR-ABL was 44.5% (45/101). BCR-ABL positive patients had a significantly lower survival ($43.7{\pm}4.24$ weeks) and higher white cell count as compared to others, except patients with MLL-AF4. The highest relapse-free survival was documented with ETV6-RUNX1 (14.2 months) followed closely by those cases in which no gene was detected (13.100). RFS with BCR-ABL, MLL-AF4, TCF3-PBX1 and SIL-TAL1 was less than 10 months (8.0, 3.6, 5.5 and 8.1 months, respectively). Conclusions: This is the first study from Pakistan correlating molecular markers with disease biology and treatment outcome in pediatric ALL. It revealed the highest reported frequency of BCR-ABL FO in pediatric ALL, associated with poor overall survival. Our data indicate an immediate need for incorporation of tyrosine kinase inhibitors in the treatment of BCR-ABL+ pediatric ALL in this population and the development of facilities for stem cell transplantation.

Production of Inulase Using Jerusalem Artichoke Tuber Extract (돼지감자 추출물을 이용한 이눌라아제 생산)

  • Choi, Weon-Sang;Choe, Yong-Kyung;Kim, Su-Il;Byun, Si-Myung
    • Applied Biological Chemistry
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    • v.27 no.4
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    • pp.238-244
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    • 1984
  • To produce inulase from Kluyveromyces fragilis No.351 using Jerusalem artichoke tuber extract, optimization of the condition was conducted. As results, the optimal concentration of artichoke tuber extract was 3.5 % and bactocasitone showed better production than yeast extract. The optimal temperature and pH were $30^{\circ}C$ and 5.5, respectively. The addition of $NH_4H_2PO_4$ increased the enzyme production. Inulase synthesis was growth-associated and the enzyme production increased as concentration of dissolved oxygen increased. A higher quantity of industrial grade inulase was prepared by the combination of ultrafiltration and ethanol precipitation with 72% recovery.

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