• Title/Summary/Keyword: Biolog GP microplate assay

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Identification of Leuconostoc strains isolated from kimchi using carbon -source utilization patterns

  • Lee, Jung Sook;Chun, Chang Ouk;Kim, Sam Bong;Park, Bong Keun;Lee, Hun Joo;Ahn, Jong Seog;Mheen, Tae Ick
    • Journal of Microbiology
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    • v.35 no.1
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    • pp.10-14
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    • 1997
  • The database of metabolic fingerprints generated using the GIolog system of lactic acid bacteria isolated from kimchi, described by Lee et al. (8), was used for the identification of 75 Leuconostoc isolates. The test strains were isoalted using a selective isolation medium specific for the genus Leuconostoc and examined for their ability to oxidize carbon sources using the Biolog system. The results show that the 75 test strains were identified to the known Leuconostoce clusters. It is suggested that the Biolog system can be applied for rapid identification of lactic acid bacteria isolated from kimchi.

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Classification of Isolates Originating from Kimchi Using Carbon-source Utilization Patterns

  • LEE, JUNG-SOOK;CHANG OUK CHUN;MIN-CHUL JUNG;WOO-SIK KIM;HONG-JOONG KIM;MARTIN HECTOR;SAM-BONG KIM;CHAN-SUN PARK
    • Journal of Microbiology and Biotechnology
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    • v.7 no.1
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    • pp.68-74
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    • 1997
  • One hundred and eighty two lactic acid bacteria, isolated mainly from kimchi, including reference strains were examined for their ability to utilize 95 carbon sources. The test strains were assigned to 5 major, 1 minor and 12 single-membered clusters based on the $S_{SM}$, UPGMA algorithm (at similarity of $80{\%}$). These aggregate clusters were equivalent to the genus Leuconostoc (aggregate cluster M and N), the genus Lactobacillus (aggregate cluster Q and R), and the genera Lactobacillus and Leuconostoc (aggregate cluster O and P) according to the database of the Biolog system. This study demonstrates that rapid identification and classification of isolates originating from kimchi can be achieved on the basis of such carbon source utilization tests.

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