• Journal of Ginseng Research
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• 제43권3호
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• pp.385-393
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• 2019

Background: The root and rhizome are historically and officially utilized medicinal parts of Panax notoginseng (PN) (Burk.) F. H. Chen, which in raw and steamed forms are used differently in practice. Methods: To investigate the differences in chemical composition and bioactivities of PN root and rhizome between raw and steamed forms, high-performance liquid chromatography analyses and pharmacologic effects evaluated by tests of anticoagulation, antioxidation, hemostasis, antiinflammation, and hematopoiesis were combined. Results: With the duration of steaming time, the contents of ginsenosides $Rg_1$, Re, $Rb_1$, Rd, and notoginsenoside $R_1$ in PN were decreased, while those of ginsenosides $Rh_1$, $20(S)-Rg_3$, $20(R)-Rg_3$, $Rh_4$, and $Rk_3$ were increased gradually. Raw PN samples steamed for 6 h at $120^{\circ}C$ with stable levels of most constituents were used for the subsequent study of bioeffects. Raw PN showed better hemostasis, anticoagulation, and antiinflammation effects, while steamed PN exhibited stronger antioxidation and hematopoiesis activities. For different parts of PN, contents of saponins in PN rhizome were generally higher than those in the root, which could be related to the stronger bioactivities of rhizome compared with the same form of PN root. Conclusion: This study provides basic information about the chemical and bioactive comparison of PN root and rhizome in both raw and steamed forms, indicating that the change of saponins may have a key role in different properties of raw and steamed PN.

• 한국전자파학회논문지
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• 제14권12호
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• pp.1311-1320
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• 2003

본 논문에서는 RF 전자기장을 사용한 동물 및 세포 실험에서 전자기장 노출량의 정확성을 평가할 수 있는 방법을 제안하였다. 이를 위해 전자파 흡수율(SAR)을 전자기장 노출량의 단위로 사용한 동물 및 세포 연구 논문과 휴대폰 및 PDA에 대한 전자파 흡수율 시험 성적서를 대상으로 출력 전력, 전력 밀도 등과 SAR의 상관 관계 및 회귀 관계를 분석하였다. 동물 실험의 경우 전력 밀도와 SAR이, 세포 실험의 경우 출력 전력이 duty factor를 고려한 SAR과 통계적으로 유의한 상관 관계를 보였다. 회귀분석에서의 노출 불확실성을 평가하기 위해 결정계수값을 분석하였다. 각각의 실험장치 및 방법에 대한 해석 또는 측정 기법의 분석 이전에 본 연구 방법을 실시하여 대상 연구군의 특성을 고찰한다면 보다 효율적인 전자기장노출신뢰성 평가를 실시할 수 있을 것으로 판단된다.

• Advances in environmental research
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• 제4권1호
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• pp.39-48
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• 2015

Bisphenol A is widely used in plastic and other industrial consumer products. Release of bisphenol A and its analogues into the aquatic environment during manufacture, use and disposal has been a great scientific and public concern due to their toxicity and endocrine disrupting effects on aquatic wildlife and even human beings. More recent studies have shown that these alkylphenols may affect the molting processes and survival of crustacean species such as American lobster, crab and shrimp. In this study, we have developed gas chromatography with flame ionization detection (GC-FID) and gas chromatography-mass spectrometric (GC-MS) methods for the determination of bisphenol A and its analogues in shrimp Macrobrachium rosenbergii, blue crab Callinectes sapidus and American lobster Homarus americanus samples. Bisphenol A, 2,4-bis-(dimethylbenzyl)phenol and 4-cumylphenol were found in shrimp in the concentration ranges of 0.67-5.51, 0.36-1.61, and < LOD (the limit of detection)-1.96 ng/g (wet weight), and in crab of 0.10-0.44, 0.13-0.62, and 0.26-0.58 ng/g (wet weight), respectively. In lobster tissue samples, bisphenol A, 2-t-butyl-4-(dimethylbenzyl)phenol, 2,6-bis-(t-butyl)-4-(dimethylbenzyl)phenol, 2,4-bis-(dimethybenzyl)phenol, 2,4-bis-(dimethylbenzyl)-6-t-butylphenol and 4-cumylphenol were determined at the concentration ranges of 4.48-7.01, 1.23-2.63, 2.71-9.10, 0.35-0.91, 0.64-3.25, and 0.44-1.00 ng/g (wet weight), respectively. At these concentration levels, BPA and its analogs may interfere the reproduction and development of crustaceans, such as larval survival, molting, metamorphosis and shell hardening.