• 대한원격탐사학회지
• /
• 제39권5_1호
• /
• pp.655-667
• /
• 2023

전 세계적으로 녹조 대발생은 빈번하게 보고되고 있으며, 국내에서도 매년 녹조로 인한 심각한 수질 오염 문제가 발생하고 있다. 지속적인 관리와 신속한 대응을 통한 수생태계 보호가 필요하다. 녹조 발생의 지표인 chlorophyll-a (Chl-a) 농도를 예측하기 위해 위성 영상을 이용한 연구가 많이 이루어지고 있다. 하지만 수계에 따라 변하는 분광특성과 대기 보정 오류로 인해 정확한 Chl-a 산출에 어려움이 있어 최근 머신러닝 모델을 활용하고 있다. 위성 분광지수 뿐만 아니라 녹조에 영향을 미치는 인자들에 대한 복합적인 고려가 필요하다. 따라서, 본 연구는 수질, 수문 및 기상 인자와 Sentinel-2 영상을 복합적으로 고려하여 데이터셋을 구축하였다. 최근 5년간 낙동강에 위치한 8개 보 구간의 Chl-a 농도 예측에 대표적인 앙상블 모델 random forest (RF)와 extreme gradient boosting (XGBoost)을 활용하였다. 모델 평가 지표로 r-squared score (R²), root mean square errors(RMSE), mean absolute errors (MAE)를 사용하였으며, XGBoost의 R²가 0.810, RMSE가 6.612, MAE가 4.457로 유의미한 결과를 얻은 것을 확인하였다. Shapley additive explanations (SHAP) 분석을 통해 두 모델 모두 수질 인자 suspended solids (SS), biochemical oxygen demand (BOD), dissolved oxygen (DO)과 red edge 밴드를 활용한 밴드비가 높은 중요도를 보인 것을 알 수 있었다. 다양한 입력 데이터는 모델 성능 향상에 도움을 주는 것을 확인할 수 있었으며, 국내외 녹조 탐지에 적용될 수 있을 것으로 보인다.

• 한국수정란이식학회지
• /
• 제27권2호
• /
• pp.93-100
• /
• 2012

To investigate the biochemical nature of changes in vaginal physiology during estrus and pregnancy, we examined the cytology and viscosity, and monitored the protein expression profile in vaginal mucus during estrus and pregnancy. The viscosity progressively decreased from estrus to pregnancy. Cell type analysis revealed that white blood cells progressively increased from estrus to pregnancy, while red blood cells progressively decreased during pregnancy. The cornification index (CI) was higher in estrus than in pregnancy. Protein mass spectrumetry identified the presence of ribosome-binding protein 1, GRIP 1 (Glutamate receptor-interacting protein 1)-associated protein 1, DUF729 (Domain of unknown function729) domain-containing protein 1, prolactin precursor, dihydrofolatereductase, and MMP (Matrix metalloprotease)-9 in vaginal mucus. MMP-2 and MMP-9 proteins in the vaginal mucus were active throughout estrus and gestation, as measured by a gelatinase assay, but most abundant in the vaginal mucus on day 0 of estrus. Results from ELISA of MMP-2 and MMP-9 were in accordance with the gelatinase assay. In light of the crucial role of metalloproteinases in extracellular matrix remodeling, the level of MMP-9 in vaginal mucus might be useful as an indicator of estrus and pregnancy to increase the efficiency of reproduction.

• Journal of the Korean Data and Information Science Society
• /
• 제26권4호
• /
• pp.813-826
• /
• 2015

본 연구는 화학 반응 모형의 추정 문제를 다루고 있다. 화학 반응 모형이란 생화학 분야에서 종(species) 들 간의 상호작용을 통한 변화 과정을 설명하기 위한 모형으로 생화학 분야 뿐 만 아니라 질병의 확산과정을 설명하는데 적용하는 모형이다. 본 연구에서는 화학 반응 모형 안에서 종들의 움직임이 확률적이라는 가정하에 Gillespie 알고리즘을 이용하여 모형 추정을 위한 우도함수를 구축하였다. 제한적인 자료구조 하에서 베이지안 접근법에 기반하여 MCMC (Markov chain Monte Carlo)방법에 기반한 모수의 추정 방법을 제안하였다. 제안된 방법들은 생태계 포식자-피식자 관계를 설명하기 위한 Lotka-Volterra 모형과 유전자 전사 (gene transcription) 과정을 설명하기 위한 L1 retrotransposition 모형에 적용하였다. 그 결과 우수한 추정 결과를 보였다.

• 한국자기공명학회논문지
• /
• 제3권1호
• /
• pp.1-11
• /
• 1999

Localized in vivo 31P NMR spectroscopy was applied to evaluate the postmortem catabolism of high energy phosphates in rabbit skeletal muscle tissue. In the premortem processes all of the important high energy phosphate metabolites were characterized, and particularly phosphocreatine (PCr) resonance signal was the strongest. In the immediate phases of the postmortem processes the signal intensities of PCr, phosphomonoesters (PME), phosphodiesters(PDE), $\alpha$-, $\beta$- and ${\gamma}$-adenosine triphosphate (ATP) resonance began to decrease while the signal intensity of inorganic phosphorus (Pi) resonance began to increase. The present study suggests that localized in vivo 31P NMR spectroscopy may provide more precise biochemical information of the early postmortem period based on the metabolic alterations of phosphate. The unique ability of localized in vivo 31P NMR spectroscopy to offer noninvasive information about tissue biochemistry in animals as well as human may have an impact on thanatochronology and medicolegal science.

• 대한전자공학회논문지SD
• /
• 제45권12호
• /
• pp.8-14
• /
• 2008

본 논문에서는 $8{\times}8$ CNT 센서 어레이를 CMOS 공정 후 처리를 통하여 센서회로가 제작된 CMOS 칩에 집적시켜 측정장비 없이도 자체적으로 감지결과를 출력할 수 있는 센서 칩의 기본적인 플랫폼을 설계 제작한 결과를 보고한다. 센서 소자로는 알루미늄 패드 사이에 연결된 CNT network을 사용하였으며 생화학적 반응에 의하여 전기전도도가 변화하는 것을 감지한다. 표준 CMOS 공정의 감지회로는 CNT network의 저항 값 변동에 의해 ring oscillator의 주파수가 변동하는 것을 감지하는 방식을 사용한다. 제작된 CMOS 센서 칩을 활용하여 이를 대표적인 생화학물질인 glutamate을 검출하는데 실험적으로 적용하여 농도에 따른 출력결과 값을 얻는데 성공한다. 본 연구를 통하여 본 센서 칩 플랫폼을 이용한 상용화의 가능성을 확인하며, 추가적으로 개발이 필요한 기술에 대해 파악한다.

• Asian Pacific Journal of Cancer Prevention
• /
• 제15권8호
• /
• pp.3567-3570
• /
• 2014

Background: Hepatocellular carcinoma (HCC) is one of the most common cancers in men and the third most common cancer in woman in Thailand. This retrospective study was designed to assess the prevalence, clinical manifestations, treatment outcomes and prognosis of HCC in the central region of Thailand. Materials and Methods: The authors retrospectively reviewed all HCC patients aged more than 15 years old in Thammasat university hospital (TUH) during the period from January 2007 to December 2012. Clinical information, biochemical tests and radiologic findings were collected from review of medical records. Results: There were 308 patients with HCC, which accounted for the prevalence of 5.19% of all cancers diagnosed in TUH during the study period. Of these, 125 (40.5%) had complete information retrievable from their medical records and met the inclusion criteria, 99 (79.2%) were males. The mean age was 57.4 years. A quarter of HCC patients in this study presented without any symptom before diagnosis. The common clinical presentations in the remaining patients were hepatomegaly 64/125 (51.2%), abdominal pain 56/125 (44.8%) and ascites 16/125 (20.8%). Cirrhosis was seen in almost all patients (92.8%). The most common causes of HCC in this study were chronic hepatitis B (49.6%) and C (19.2%). Based on Barcelona Clinic Liver Cancer staging, 75.4% presented at intermediate or late stage. Patients receiving curative therapy with either surgical treatment or radiofrequency ablation had significantly longer survival time after the HCC diagnosis than the palliative therapy group (11.0 months vs 4.0 months, p value= 0.004). The mean survival time after the HCC diagnosis was 10.5 months. Conclusions: The common causes of HCC in central region of Thailand were chronic hepatitis B and C. Surgical therapy or RFA seemed to provide better outcomes than other treatments but only in patients with early stage lesions. Most of the patients in this study presented with advanced diseases and had grave prognosis. Appropriate screening patients at risk for HCC might be an appropriate way to achieve early diagnosis and improve the treatment outcome.

• Asian-Australasian Journal of Animal Sciences
• /
• 제18권7호
• /
• pp.922-926
• /
• 2005

The present study was aimed to investigate proteome affected by Panax ginseng extracts in chicken muscles. The whole muscle proteins from chicken fed boiled extracts of 0% (control), 1%, 3%, and 5% Panax ginseng in water were separated by two-dimensional electrophoresis (2-DE) gels using immobilized non-linear gradient (pH 3-10) strips. More than 300 protein spots were detected on silver staining gels. Among them, four protein spots were distinctively up-regulated by Panax ginseng treatments and further investigated by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). The obtained MS data were searched against SwissProt database using the Mascot search engine. The up-regulated proteins were finally identified as $\alpha$-tropomyosin (2 spots), triosephosphate isomerase, and one unknown protein. Based on the known functions of the identified proteins, they are highly related to muscle development and enhanced immunity in chickens. These proteins can give valuable information of biochemical roles for Panax ginseng in chicken meats.

• 센서학회지
• /
• 제17권5호
• /
• pp.369-374
• /
• 2008

A novel fiber optic surface plasmon resonance (SPR) sensor using cyclic olefin copolymer (COC) prism with the spectral modulation is presented. The SPR sensor chip is fabricated using the SU-8 photolithography, Ni-electroplating and COC injection molding process. The sidewall of the COC prism is partially deposited with Au/Cr (45/2.nm thickness) by e-beam evaporator, and the thermal bonding process is conducted for micro fluidic channels and optical fibers alignment. The SPR spectrum for a phosphate buffered saline (0.1.M PBS, pH.7.2) solution shows a distinctive dip at 1300.nm wavelength, which shifts toward longer wavelength with respect to the bovine serum albumin (BSA)concentrations. The sensitivity of the wavelength shift is $1.16\;nm{\cdot}{\mu}g^{-1}{\cdot}{\mu}l^{-1}$. From the wavelength of SPR dips, the refractive indices (RI) of the BSA solutions can be theoretically calculated using Kretchmann configuration, and the change rate of the RI was found to be $2.3{\times}10^{-5}RI{\cdot}{\mu}g^{-1}{\cdot}l^{-1}$. The realized fiber optic SPR sensor with a COC prism has clearly shown the feasibility of a new disposable, low cost and miniaturized SPR biosensor for biochemical molecular analyses.

• Nutrition Research and Practice
• /
• 제13권3호
• /
• pp.222-229
• /
• 2019

BACKGROUND/OBJECTIVES: Aging is an imperative problem for many countries in this century, and presents several challenges for the maintenance of good nutritional status. This study aims to assess the impact of socio-demographic factors, lifestyle and health status on the nutritional status among the elderly in Taiwan. SUBJECTS/METHODS: A cross-sectional study was carried out in Taiwan. Data were obtained from the Mei Jau Health Management Institution, which is a private health evaluation provider with multiple health screening centers in Taiwan and Asia. This study included 7947 adults aged 65 years or above. The data were extracted between 2001 to 2010. Nutritional status was assessed using anthropometric data, biochemical data and dietary intake information. RESULTS: Among the 7947 participants with mean age of 70.1 (SD = 4.5) years, 20.2%, 6.6%, 10.5% and 52.5% experienced underweight, protein malnutrition, anemia and inadequate dietary intake in the past month, respectively. Age was negatively correlated with body weight (r = -0.19, P = 0.02), body mass index (r = -0.41, P < 0.001), albumin level (r = -0.93, P < 0.001) and hemoglobin level (r = -0.30, P = 0.008). Age above 70 years, gender, unmarried status, retirement, lack of education, low family income, smoking, alcohol drinking, sleep duration of 6-8 hours, vegetarian diet, multiple medications, comorbidity and dysphagia were positively associated with malnutrition in older adults. CONCLUSIONS: Underweight and inadequate dietary intake are prevalent among the elderly in Taiwan. Vegetarian diet, multiple medications, comorbidity, dysphagia and lifestyle factors such as smoking, alcohol drinking and sleep duration of 6-8 hours are risk factors for undernutrition in older adults.

• Journal of Animal Science and Technology
• /
• 제46권6호
• /
• pp.887-896
• /
• 2004

제주말의 혈통보존을 위한 기초자료를 마련할 목적으로 국내에서 사육중인 몽고말 19두를 대상으로 적혈구항원형 및 혈액단백질형의 표현형 분포와 유전자 빈도를 조사한 결과는 다음과 같다. 적혈구항원형의 표현형은 $A^af$(42.1%), $C^a$(89.5%), $K^-$((84.2%), $U^a$(63.2%), $P^a$(42.1%) $P^-$(42.1%), $Q^c$(31.6%), $Q^-$(31.6%)에서 높은 빈도를 나타냈으며 적혈구항원형 D 시스템의 유전자형은 $D^cgm/dghm$ (15.8%), $D^dghm/dghm$(15.8%), $D^ad/dghm$(10.5%), $D^ade/dghm$ (10.5%)가 높은 빈도 순으로 관찰되었다. 혈액단백질형 7개의 좌위에서는 $AL^{AB}$(52.6%), $AIB^K$( (89.5%), $ES^I$(63.2%), $GC^F$(78.9%), $HB^{BI}$(68.4%),$PGD^F$(84.2%), $TF^{F1R}I$(21.1%), $TF^{F2R}I$(21.1%)로 높은 빈도를 보였으며, $HB^{A2B2}I$(5.3%), $PGD^{DS}I$(5.3%)가 특이하게 관찰되었다. 적혈구항원형의 유전자 빈도를 조사한 결과 $A^a$(0.4211), $C^a$(0.8947), $K^-$(0.8421), $U^a$(0.6316), $P^a$ (0.4474), $Q^c$(0.4474), $D^{dghm}$(0.4211)로 가장 높은 빈도를 보였으며, 혈액단백질형 7개 좌위에 대해서 유전자 빈도를 조사한 결과 $AL^B$(0.6579), $AIB^K$(0.9211), $ES^I$(0.7895), $GC^F$(0.8947), $HB^BI$ (0.7895), $PGD^F$(0.8421), $TF^R$(0.3421)가 높은 빈도로 관찰되었다.