• Title/Summary/Keyword: Biochemical information

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Transgenic plants with cyanobacterial genes

  • Park, Youn-Il;Choi, Sang-Bong;Liu, Jang R.
    • Plant Biotechnology Reports
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    • v.3 no.4
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    • pp.267-275
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    • 2009
  • Over the years, cyanobacteria have been regarded as ideal model systems for studying fundamental biochemical processes like oxygenic photosynthesis and carbon and nitrogen assimilation. Additionally, they have been used as human foods, sources for vitamins, proteins, fine chemicals, and bioactive compounds. Aiming to increase plant productivity as well as nutritional values, cyanobacterial genes involved in carbon metabolism, fatty acid biosynthesis, and pigment biosynthesis have been intensively exploited as alternatives to homologous gene sources. In this short review, transgenic plants with cyanobacterial genes generated over the last two decades are examined, and the future prospects for transgenic crops using cyanobacterial genes obtained from functional genomics studies of numerous cyanobacterial genomes information are discussed.

A Case Report of Severe Melosalgia with Aconiti Tuber Toxicity (하지 통증을 주요 증상으로 한 부자 중독 환자에 대한 증례 보고)

  • Kim, Jin-Hee;Kim, Jin-Mi;Jo, Jeong-Hyo;Son, Chang-Gue
    • The Journal of Korean Medicine
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    • v.32 no.5
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    • pp.126-133
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    • 2011
  • Objective: We report a case of non-specific Aconiti Tuber poison complaining only of severe peripheral neurotoxicity without cardiac dysfunction. Methods: The authors evaluated the symptom changes of a patient who was hospitalized in an Oriental hospital for fourteen days. The patient received acupuncture, herbal medicine, moxibustion and analgesics. Result: No abnormality in examination for cardiac function or biochemical parameters was present. The severity of pain and dysesthesia in lower extremities gradually receded during the period of treatment with herbal and western medicines. Conclusion: This study provides helpful information for treatment of Aconiti Tuber toxicity.

Factors Affecting and Techniques to Quantify $CH_4\;and\;N_2O$ Emissions from Stored Liquid Manure

  • Park, K.H.;Wagner-Riddle, Claudia
    • Journal of Animal Environmental Science
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    • v.13 no.1
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    • pp.1-12
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    • 2007
  • Stored animal manure is considered as a significant agricultural source of methane $(CH_4)$ and nitrous oxide $(N_2O)$ which have 23 and 297 times higher global warming effect when compared to carbon dioxide $(CO_2)$. Uncertainties caused by lack of understanding physical and biochemical environment in stored animal manure and by errors of emission measurement methods, even though many researches measuring $CH_4\;and\;N_2O$ emissions from stored manure have been conducted for a few decades. In this paper, general information of $CH_4\;and\;N_2O$ generation and emissions from stored animal manure and the measurement methods for quantifying $CH_4\;and\;N_2O$ emissions are discussed.

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Kinetic Analysis of the MAPK and PI3K/Akt Signaling Pathways

  • Suresh, Babu CV;Babar, Sheikh Md. Enayetul;Song, Eun Joo;Oh, Eulsik;Yoo, Young Sook
    • Molecules and Cells
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    • v.25 no.3
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    • pp.397-406
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    • 2008
  • Computational modeling of signal transduction is currently attracting much attention as it can promote the understanding of complex signal transduction mechanisms. Although several mathematical models have been used to examine signaling pathways, little attention has been given to crosstalk mechanisms. In this study, an attempt was made to develop a computational model for the pathways involving growth-factor-mediated mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3'-kinase/protein kinase B (PI3K/Akt). In addition, the dynamics of the protein activities were analyzed based on a set of kinetic data. The simulation approach integrates the information on several levels and predicts systems behavior. The in-silico analysis conducted revealed that the Raf and Akt pathways act independently.

Studies on the m-Toluate Degradating Plasmid in Pseudomonas (m-Tluate를 분해하는 Peudomonas의 분리 및 Dgradative Pasmid와의 연관성에 관하여)

  • 박순희;하영칠;홍순우
    • Korean Journal of Microbiology
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    • v.17 no.1
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    • pp.25-41
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    • 1979
  • A strain able to grow up m-toluate minimal medium has been isolated after selective enrichment and given the name T81X, which was later identified as pseudomonase putida according to its morphological and biochemical characteristics. After treatment with plasmied specific curing agent, mitomycin C, followed by replica plating on m-toluate and xylene minimal agar plate, T81Xstrain has been shown to harbour a curable plasmid relating to the m-toluate and xylene metabolism. Spontaneous curing frquency of this plasmid was also greatly enhanced by growing on benzoate minimal medium. After then, it was also xylene metabogrowing on benzoate minimal medium. After then, it was found to be conjugally nontransmissible. From the comparative investigation of catechol 1,2-oxygenase and catechol 2,3-oxygenase activities in wild type and cured strain on various growth substrate, it appeared that T81X strain has both of these two enzymes while cured strain has catechol 1,2-oxygenase only. Growing on m-toluate minimal medium T81X strain should carry the genetic information necessary for coding the catechol 1,2-oxygense induced by m-toluate or benzoate, on that curable plasmid.

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Studies on the Seed's Germinability test by the T.T.C. testing method. (T.T.C 검정방법에 의한 종자의 발아력검정에 관한 연구)

  • 전우방
    • Asian Journal of Turfgrass Science
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    • v.2 no.1
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    • pp.70-77
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    • 1988
  • In order to determine and to reduce the differences between biochemical test method for the seed viability with T.T.C. (2, 3, 5. Triphenyl tetrazolium Chloride) reagent and germinator method, the topographical diagram of red colored formazan was carried out in 15-16 parts differences. From this resulted information, the classification of the typical staining reaction given for 3 species were derived into 3-4 parts for the germinable in normal seedling, the same as the following results. 1. Corn (Gram inaceae) * Entier embryo stained in bright red color. * Both extremities of scutellum unstained * Both extremities of scuttlium, coleorhiza and non-critial portions of radicle unstained. 2. Soybean (Leguminosae) *Seed completely stained in red color. *Minor unstained areas on cotyledons. *Extreme tip of radicle unstained; minor unstained areas on cotyledons 3. Radish (Cruciferae) *Seed completely stained. *Minor unstained areas on cotyledons. *Outer cotyledon mostly unstained: inner cotyledon completely stained Extreme tip of radicle unstained: large portion of outer cotyledon unstained.

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Fat Cell Formation and Obesity-Related Diseases

  • Kawada, Teruo
    • Preventive Nutrition and Food Science
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    • v.8 no.1
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    • pp.105-112
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    • 2003
  • Animals possess a highly sophisticated mechanism of storing energy in adipose tissue inside their bodies. However, in humans it has been clarified that adipocyte (fat cell), which composes the body fat (adipose) tissues, development and the extent of subsequent fat accumulation are closely associated with the occurrence and advancement of various common diseases (e.g., type-2 diabetes, coronary artery disease, and hypertension) resulting from obesity. Recent exciting progress in clinical and biochemical studies of adipocytes has rapidly clarified the functions of adipocytes and adipose tissue. Interesting findings are the function of white adipocytes as "secreting cells" and the molecular mechanism undelying adipocyte differentiation at the transcriptional level in relation to nuclear receptors. Consequently, the adipose tissue is being targeted for the prevention or treatment of many common diseases. In this review, I will focus on recent information on characteristics of adipocytes and the relationship between obesity and common obesity-related diseases. diseases.

Characterization of Trueperella pyogenes isolated from caseous lymphadenitis lesions in Korean native goats

  • Aftabuzzaman, Md.;Espiritu, Hector;Kim, Seon-Ho;Mamuad, Lovelia;Jin, Su-Jeong;Lee, Sang-Suk;Cho, Yong-Il
    • Korean Journal of Veterinary Service
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    • v.44 no.4
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    • pp.321-326
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    • 2021
  • The study characterized Trueperella pyogenes (T. pyogenes) from caseous lymphadenitis (CLA) in Korean native goats (KNG). CLA samples (n=191) were obtained from abattoir-slaughtered KNG. Pus were obtained and Corynebacterium pseudotuberculosis was detected by PCR using PLD gene. Three PLD-negative samples (1.57%) from supramammary lymph node were subjected to bacterial culture. Isolates were identified using 16S rRNA and biochemically characterized by APIZYM. Phylogenetic and biochemical analyses identified the isolates as T. pyogenes, and have shown separate clusters and varied enzyme reaction, suggesting genotypic and phenotypic diversity. This first report of T. pyogenes-induced CLA in KNG provided information for further investigations.

A report of 10 unrecorded bacterial species of Korea, isolated from agricultural soil in 2022

  • Oung Bin Lim;Ji Soo Lee;Hyosun Lee;Ki Eun Lee;In Tae Cha;Won Jae Chi;Dong-Uk Kim
    • Korean Journal of Environmental Biology
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    • v.41 no.3
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    • pp.215-222
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    • 2023
  • In 2022, research for native prokaryotic species in Korea reported 10 unrecorded bacterial strains affiliated to phyla Actinomycetota, Bacillota, and Pseudomonadota. The strains formed monophyletic clades with the most closely related species (with ≥98.7% sequence similarity) in the 16S rRNA gene sequencing. Among them, four species of the phylum Actinomycetota, two species of the phylum Bacillota, and four species of the phylum Pseudomonadota have not been reported in Korea, suggesting unrecorded species in Korea. Information on strains such as Gram staining reaction, colony and cell morphology, biochemical characteristics, and isolation sources were provided in the species description.

A Nutrition Status Analysis System Based on Hierarchical Fuzzy Inference Approach (계층적인 퍼지추론 기법을 기반으로 한 영양상태 분석시스템)

  • Son, Chang-S.;Jeong, Gu-Beom
    • Journal of the Korean Institute of Intelligent Systems
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    • v.17 no.6
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    • pp.731-737
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    • 2007
  • In this paper, we propose a system for analyzing nutrition status based on hierarchical fuzzy inference approach, where the hierarchical fuzzy approach used to analyze the transition process on the nutritional status from an obesity degree, the previous nutritional status, and the eating pattern with an individual. Moreover we discussed about the selection method of fuzzy membership intervals of the next layer to improve the reliability of inference results in hierarchical fuzzy system, where their intervals are modified by using statistical information of the defuzzified results obtained from the previous layer. To show the effectiveness of this system, we evaluated the nutritional status from the information of anthropometric measurement, biochemical test, and INQ on 113 people over the age of 65, and also analyzed their nutritional status.