• 제목/요약/키워드: Biochemical alteration

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Influence of Microbial Activity on the Long-Term Alteration of Compacted Bentonite/Metal Chip Blocks

  • Lee, Seung Yeop;Lee, Jae-Kwang;Kwon, Jang-Soon
    • 방사성폐기물학회지
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    • 제19권4호
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    • pp.469-477
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    • 2021
  • Safe storage of spent nuclear fuel in deep underground repositories necessitates an understanding of the long-term alteration of metal canisters and buffer materials. A small-scale laboratory alteration test was performed on metal (Cu or Fe) chips embedded in compacted bentonite blocks placed in anaerobic water for 1 year. Lactate, sulfate, and bacteria were separately added to the water to promote biochemical reactions in the system. The bentonite blocks immersed in the water were dismantled after 1 year, showing that their alteration was insignificant. However, the Cu chip exhibited some microscopic etch pits on its surface, wherein a slight sulfur component was detected. Overall, the Fe chip was more corroded than the Cu chip under the same conditions. The secondary phase of the Fe chip was locally found as carbonate materials, such as siderite (FeCO3) and calcite ((Ca, Fe)CO3). These secondary products can imply that the local carbonate occurrence on the Fe chip may be initiated and developed by an evolution (alteration) of bentonite and a diffusive provision of biogenic CO2 gas. These laboratory scale results suggest that the actual long-term alteration of metal canisters/bentonite blocks in the engineered barrier could be possible by microbial activities.

Post Infection Physiobiochemical Alteration at Various Intensities of Leaf spot (Myrothecium roridum) in Mulberry

  • Kumar, P.M.Pratheesh;Qadri, S.M.H.;Pal, S.C.;Mishra, A.K.;Urs, S.Raje
    • International Journal of Industrial Entomology and Biomaterials
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    • 제7권2호
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    • pp.175-180
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    • 2003
  • Changes in biochemical constituents and physiological alteration were studied in various intensities (1-5%, 6-15%, 16-30%, 31-50% and > 50%) of leaf spot (Myrothecium roridum) on mulberry leaves and compared with healthy leaves. Chlorophyll, total soluble sugar and total protein were decreased (P < 0.01), but total phenol increased due to pathogen infection. Changes in biochemical constituents showed significant correlation with intensity of disease. Chlorophyll ($r^2$= 0.92), and protein (($r^2$= 0.83) possessed negative while phenol (($r^2$= 0.61) possessed positive correlation. Photosynthetic rate, transpiration rate, stomatal conductance, moisture content (%) and physiological water use efficiency (pWUE) were decreased, but stomatal resistance increased in the infected leaves. Physiological parameters also possessed significant (P < 0.01) correlation with disease intensity. Photosynthetic rate (($r^2$= 0.96), transpiration rate ($r^2$=0.88), stomatal conductance (($r^2$= = 0.65), physiological water use efficiency (($r^2$= 0.88) and moisture content (r = 0.85) were negatively but stomatal resistance (($r^2$= 0.75) was positively correlated to disease intensities.

Insecticidal Efficacy of Porteresia coarctata (Roxb.) on Bio-chemical Alteration of Spodoptera litura (Fab.)

  • Bhattacharyya Atanu;Goswami Arunava;Dihidar Shankar;Adhikari Sujit;Banerjee Sudip Kumar;Talapatra Soumendra Nath;Barik Bikas Ranjan;Bhattacharya Subir Kumar;Chandra Asit Kumar
    • International Journal of Industrial Entomology and Biomaterials
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    • 제14권1호
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    • pp.9-13
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    • 2007
  • Hexane extract of Porteresia coarctata (Roxb.) exhibits a toxic effect on the tissues of Spodoptera litura (F) while fed at the dose of 1000 and 2000 ppm thoroughly mixing with castor leaves (Ricinus communis L) after dissolving in DMSO at late fourth instar whereas only DMSO treated castor leaves were fed to control group. The larvae were put to rear at $28^{\circ}C{\pm}1^{\circ}C$, $76{\pm}4%$ R.H. under 12 L + 12 D photoperiodic regime. In test group insects substantial reduction of protein and DNA content was marked in fat body and midgut tissues compared to DMSO treated control group. The significant biochemical alterations in the midgut tissues and fat body of test group insects indicate the insecticidal property of the said plant extract that could be tested in facilitating the phenomenal stride in Integrated Pest Management.

Effects of induced heat stress on temperature response and biochemistry: alteration of biochemical constituents in Holstein calves by heat stress

  • Lim, Hyun-Joo;Ki, Kwang-Seok
    • 농업과학연구
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    • 제46권3호
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    • pp.637-643
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    • 2019
  • The aim of the present study was to evaluate the effect of the induced heat stress on physiological response and serum biochemical parameters involving glucose, cholesterol, blood urea nitrogen (BUN), non-esterified fatty acids (NEFA), and cortisol in Holstein calves. Ten calves were kept in a climate controlled room (air temperature $37^{\circ}C$ and 90% humidity from 09:00 to 19:00) for three days. Those animals were given a one-day adaptation period. During the treatment period, we measured the skin temperature six times. Following the treatment periods, blood samples were collected before the experiment began (09:00) and at the end of the stress period (19:00). To aid analysis of the biochemical parameters, also we monitored the rectal temperature. The results, exhibited that both rectal and skin temperature showed increase in the heat stress-induced animals as compared with unstressed animals. Moreover, we noticed that the levels of BUN and NEFA increased in the blood serum of heat stress induced animals when compared with un-stressed ones. From these results, we concluded that the physiological and biochemical changes in the calves were induced by heat stress. Hence, the present study findings could be employed as base line data for development of stress reduction techniques in the dairy industry.

Utilization of Food Sources Before and After the Tsunami in Nuttallia olivacea at Gamo Lagoon, Japan

  • Shin, Woo-Seok;Nishimura, Osamu
    • Environmental Engineering Research
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    • 제18권4호
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    • pp.259-265
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    • 2013
  • This study was conducted June 2011 at Gamo Lagoon, after tsunami of March 2011, to estimate food sources and utilization. The results show that the tsunami affected the sediment properties by changing the physical environmental alterations. The fatty acids of the gut content of Nuttallia olivacea mostly comprised the same organic matter found in the sediment. Fatty acids in the tissues showed mainly diatoms, bacteria, and dinoflagellates. That is, most of the food sources (i.e., diatoms, bacteria, dinoflagellates, macroalgae, and terrestrial organic matter) probably pass through the digestive system unharmed; however, terrestrial organic matter, which is refractory to biochemical degradation, indicated a different assimilation trend between the gut content and the tissue. This result suggests that input of labile organic matter from the sediment may control selective metabolism in N. olivacea. From these results, although the physical environment of sediment characteristics by tsunami changed, the food utilization of N. olivacea suggested a better assimilation of selected components from the gut content, irrespective of physical alteration.

Effect of variable degrees of jejunal resection upon different clinico-biochemical parameters in dogs

  • Dilawer, Muhammad Sohail;Khan, Muhammad Arif;Abidin, Zain ul;Azeem, Shahan;Majeed, Khalid Abdul;Shahbaz, Adeel;Khan, Aamir Riaz
    • 대한수의학회지
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    • 제51권4호
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    • pp.309-313
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    • 2011
  • Dogs are considered to be the best companions of human beings due to their loyalty, obedience and pleasant disposition. Jejunum is the largest part of small intestine mainly involved in absorption of nutrients. Jejunal resection up to 80% allows normal weight gain while resection up to 90% increased morbidity and mortality. In the present study, 20 dogs were divided into 4 groups based on the degree of jejunal resection i.e. A (70% resection), B (80% resection) and C (100% resection) while group D served as control. Dogs in the 70% and 80% jejunal resection group showed normal growth and function while 100% jejunal resection resulted in weight loss and alteration of hematological and biochemical parameters.

Cordycepin from Medicinal Fungi Cordyceps militaris Mitigates Inflammaging-Associated Testicular Damage via Regulating NF-κB/MAPKs Signaling in Naturally Aged Rats

  • Kopalli, Spandana Rajendra;Cha, Kyu-Min;Cho, Jae Youl;Kim, Si-Kwan;Koppula, Sushruta
    • Mycobiology
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    • 제50권1호
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    • pp.86-95
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    • 2022
  • Inflammaging in male reproductive organs covers a wide variety of problems, including sexual dysfunction and infertility. In this study, the beneficial effects of cordycepin (COR), isolated from potential medicinal fungi Cordyceps militaris, in aging-associated testicular inflammation and serum biochemical changes in naturally aged rats were investigated. Male Sprague Dawley rats were divided into young control (YC), aged control (AC), and COR (5, 10, and 20 mg/kg) treated aged rat groups. Aging-associated serum biochemical changes and inflammatory parameters were analyzed by biochemical assay kits, Western blotting, and real-time RT-PCR. Results showed a significant (p < 0.05) alteration in the total blood cell count, lipid metabolism, and liver functional parameters in AC group when compared with YC group. However, COR-treated aged rats ameliorated the altered biochemical parameters significantly (p < 0.05 and p < 0.01 at 5, 10, and 20 mg/kg, respectively). Furthermore, the increase in the expression of inflammatory mediators (COX-2, interleukin (IL)-6, IL-1β, and tissue necrosis factor-alpha) in aged rat testis was significant (p < 0.05) when compared with YC group. Treatment with COR at 20 mg/kg to aged rats attenuated the increased expression of inflammatory mediators significantly (p < 0.05). Mechanistic studies revealed that the potential attenuating effects exhibited by COR in aged rats was mediated by regulation of NF-κB activation and MAPKs (c-Jun N-terminal kinase, extracellular signal-regulated kinase 1/2, and p38) signaling. In conclusion, COR restored the altered serum biochemical parameters in aged rats and ameliorated the aging-associated testicular inflammation proving the therapeutic benefits of COR targeting inflammaging-associated male sexual dysfunctions.

랫드에서 옻나무 추출액(Rhus-II)의 4주 반복 투여 안정성 평가 (Four-Week Repeated Dose Safety Test of Rhus-II Orally Administrated to Rats)

  • 최창순;한동운
    • 한국식품위생안전성학회지
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    • 제20권1호
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    • pp.7-12
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    • 2005
  • The purpose of this study was to examine the four week repeated toxicity in Sprague-Dawley rats orally administrated with Rhus-II (water fraction of Rhus Veniciflua). In acute toxicity test, three groups (40 rats of both sex) were administrated different dosages of Rhus-II, 2 g/kg (high dosage group), 1 g/kg, 0.5 g/kg and one group (10 rats of both sex) were received by orally only saline according to the Regulation on Korea Food and Drug Administration, respectively. There was no difference in body weight change, feed intake and water consumption among different dose groups. There was no alteration in relative organ weight by the administration of Rhus-II. No death of abnormal clinical signs was observed during the experimental period. Between the groups orally administered Rhus-II and the control group, there was no statistical significance in urinalysis, hematological test or serum biochemical values. There were no gross findings at final sacrifice. There was no evidence of histopathological alteration mediated by four week treatment with Rhus-II. These results suggest that no observable effect level(NOEL) of the test orally administration was considered to be more than 2g/kg in rats under the conditions employed in this study.