• 제목/요약/키워드: Bioanalysis

검색결과 227건 처리시간 0.043초

Kinetic Analysis of the MAPK and PI3K/Akt Signaling Pathways

  • Suresh, Babu CV;Babar, Sheikh Md. Enayetul;Song, Eun Joo;Oh, Eulsik;Yoo, Young Sook
    • Molecules and Cells
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    • 제25권3호
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    • pp.397-406
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    • 2008

  • Computational modeling of signal transduction is currently attracting much attention as it can promote the understanding of complex signal transduction mechanisms. Although several mathematical models have been used to examine signaling pathways, little attention has been given to crosstalk mechanisms. In this study, an attempt was made to develop a computational model for the pathways involving growth-factor-mediated mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3'-kinase/protein kinase B (PI3K/Akt). In addition, the dynamics of the protein activities were analyzed based on a set of kinetic data. The simulation approach integrates the information on several levels and predicts systems behavior. The in-silico analysis conducted revealed that the Raf and Akt pathways act independently.

  • KSCI

Simultaneous determination of sildenafil and its active metabolite in human plasma using LC/MS/MS

  • Ji, Hye-Young;Kim, Sook-Jin;Lee, Hong-Il;Lee, Seung-Seok;Lee, Hye-Sook
    • 대한약학회:학술대회논문집
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    • 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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    • pp.402.1-402.1
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    • 2002

  • The LC/MS/MS method for the simultaneous determination of sildenafil and its active metabolite N-demethylsildenafil in human plama was developed. Sildenafil. its active metabolite and the internal standard. DA-8159 were extracted form human plasma by liquid-liquid partitioning. A reverse-phase HPLC separation was performed on Luna phenylhexyl column with the mixture of acetonitrile-5 mM ammonium formate (55:45. v/v) as mobile phase. (omitted)

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Release of the Pro-inflammatory Cytokines and Facilitation of Immune Response in LPS-induced Activation of Macrophage by Crude Cordycepin Containing Adenosine(CCCA) from Cordyceps militaris

  • Han, Shin-Ha;Lee, Seung-Jeong;Song, Young-Cheon;Lim, Hee-Jung;Lee, Chong-Kil;Kwon, Oh-Seung;Ha, Nam-Joo;Kim, Kyung-Jae
    • 대한약학회:학술대회논문집
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    • 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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    • pp.139.2-139.2
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    • 2003

  • The in vitro effects of extracted fractions of C. militaris on the secretion of cytokines in murine macrophage cell line, RAW 264.7 were studied. F1 (crude cordycepin containing adenosine), F2 (ethanol precipitation), F3 (ethanol soluble supernatant) and F4 (fraction of through SK-1B) significantly stimulated the production of cytokine and nitric oxide (NO) on murine macrophage cell line RAW264.7. We examined how the ethanol extract of C. militaris regulates production of interleukine 1-beta(IL-1$\beta$), tumor necrosis factor-alpha (TNF-$\alpha$), and NO in vitro. (omitted)

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New Analytical Method of Methyltestosterone in Human Serum by Gas Chromatography/Maa Spectrometry for Pharmacokinetics and Bioequivalence Studies in Human Volunteers

  • Kim, Hye-Jung;Bae, Sung-Seok;Myung, Seung-Woon;Ryu, Jae-Chun;Chung, Youn-Bok;Kwon, Oh-Seung
    • 대한약학회:학술대회논문집
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    • 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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    • pp.241.3-242
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    • 2003

  • A simple, specific and sensitive method for the determination of methyltestosterone (MT) in human serum has been developed by gas chromatography/mass spectrometry with the purpose of conducting pharmacokinetic and bioequivalence studies of MT. This method involves the use of liquid-liquid extraction with diethyl ether and derivatization with MSTFA, using 1 ml of serum obtained from volunteers orally taken 50 mg MT. MT showed good resolutions in this conditions and no significant interfering peaks were observed. (omitted)

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Bioequivalence of $Enalacc^{TM}$ Tablet to $Renitec^{TM}$ Tablet(Enalapril maleate 10 mg)

  • Cho, Sung-Hee;Ha, Yong-Hwa;Hong, Sung-Je;Seo, Seong-Hoon;Rew, Jae-Hwan;Kim, Dong-Hyun;Lee, Kyung-Tae
    • 대한약학회:학술대회논문집
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    • 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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    • pp.244.3-245
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    • 2003

  • ABSTRACT-The purpose of the present study was to evaluate the bioequivalence of two enalapril maleate tablest, $Renitec^{TM}$(MSD Korea Ltd.) and $Enalace^{TM}$(Welfide Korea Ltd.), according to the guidelines of Korea Food and Drug Administration (DFDA). Twenty-four normal male volunteers, 22.33 ${\pm}$ 2.55 year in age and 66.54 ${\pm}$ 8.30 kg in body weight, were divided into two groups and a randomized 2${\times}$2 cross-over study was employed. After two tablets containing 10 mg of enalapril maleate per tablet were orally administered, blood was taken at predetermined time intervals and concentrations of enalapril in plasma were determined using LC-MS-MS. (omitted)

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